FastQCFastQC Report
Sat 31 Dec 2022
SRR5844963.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844963.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences205007
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCGTTCTACATCTCGTATGC10620.5180310916212617No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTTCTACATCTCGTATGCCGTCT9810.47852024565014856Illumina PCR Primer Index 11 (95% over 21bp)
TTAGCGGGTGACAGGGCAAAAATAAATAAATAAATGAAGTAGAAGAAGAACC2370.11560580858214597No Hit
TGGCACGGGAATATCCAGAAGCTACAAAGAACTCAAACAAATCAGCAAGAAA2340.1141424439165492No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTTCTACACTGTCTCTTATACAC2250.10975234991975884No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)