FastQCFastQC Report
Sat 31 Dec 2022
SRR5844941.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844941.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151405
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTTCTACATCTCGTATGCCGTCT9490.6267956804596941Illumina PCR Primer Index 11 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACCGTTCTACATCTCGTATGC8960.5917902314982992No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTTCTACACTGTCTCTTATACAC1920.12681219246392128No Hit
GACATAGGGCAGGAGCTGCAAACCACCCAAGGCTGCAATCTTACCAGGGAGG1730.11426306925134573No Hit
AATTATGGGAAGAAAAGGCAGAGGATGGCTGGTGAAAGGGTCCACACTTTAT1600.10567682705326772No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)