FastQCFastQC Report
Sat 31 Dec 2022
SRR5844928.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844928.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133582
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCGTTCTACATCTCGTATGC7850.587654025242922No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTTCTACATCTCGTATGCCGTCT6480.4850952972705903Illumina PCR Primer Index 11 (95% over 21bp)
GCTTCTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1830.136994505247713No Hit
CTCGAGGGGGCGGACCCCGGGCGGGCGCAGGGCGCGGGCTCCGGCGCCGCCG1750.13100567441721192No Hit
GTGTGAGGGATCTTCCTCAAGGGATAGTACACACGCCTCAAGACGTTTTTAA1710.12801125900196134No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCGTTCTACACTGTCTCTTA1640.12277103202527287No Hit
GTGTCAGGGGTCTCTTTCAGCTAGAATTGAAATATCTATAAGACGAACTGGA1630.12202242817146022No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTTCTACACTGTCTCTTATACAC1510.11303918192570855No Hit
AACGGCGGGTGAGAGTGTACTTTTACCAAAAGTTATCTAAGCCTGAGCACTT1440.10779895494902009No Hit
CAACATGGGCAAGACCAATCTGACTAACACGGTGAAACCCCATCTCTACTAA1420.1063017472413948No Hit
GGCTCTGGGCATACCTCGAGATAAAGTGGCATGTTAGTGAGGAGTTCTGATA1400.10480453953376952No Hit
AATGCAGGGCACATCTTTGCTCATGAGCCTGCCCACTTTCCCCCAATTGAGG1400.10480453953376952No Hit
TATGACGGGATCAAATACATATTATGAATATGTGTTTCAATTTTCTGCATTT1390.10405593567995687No Hit
TATTGTGGGAGCTGATATAACCTTGGGCAAATAGTTTCTCTAAGCTCAACTG1390.10405593567995687No Hit
AAGGCGGGCCGATCTGCTAGTGGGAAGGGGAAGCATTTGAGAAATATGTTCA1370.1025587279723316No Hit
TTACAGGGAAGTTCATGATCTTGAAAGGAAGTATGCTGTTCTCTATCAGCCT1350.10106152026470633No Hit
ATATGTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1350.10106152026470633No Hit
GCCATTGGGGTATTCATCAAGCTCTGAGTCTATCTCCAAGAAAATAGAGACT1340.1003129164108937No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)