FastQCFastQC Report
Sat 31 Dec 2022
SRR5844927.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844927.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132782
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCGTTCTACATCTCGTATGC7220.5437483996324803No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTTCTACATCTCGTATGCCGTCT6530.49178352487535965Illumina PCR Primer Index 11 (95% over 21bp)
GCTTCTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1780.1340543145908331No Hit
AACGGCGGGTGAGAGTGTACTTTTACCAAAAGTTATCTAAGCCTGAGCACTT1650.12426383094094079No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTTCTACACTGTCTCTTATACAC1560.11748580379870766No Hit
GTGTGAGGGATCTTCCTCAAGGGATAGTACACACGCCTCAAGACGTTTTTAA1560.11748580379870766No Hit
GTGTCAGGGGTCTCTTTCAGCTAGAATTGAAATATCTATAAGACGAACTGGA1520.11447334729104848No Hit
TTTCTAGGGAGACCAGATCCACTCCTCCATCTCTCTAGCCCTGAGGGGAGCC1480.11146089078338932No Hit
AATGCAGGGCACATCTTTGCTCATGAGCCTGCCCACTTTCCCCCAATTGAGG1480.11146089078338932No Hit
TTTTTGGGGGATGGTCTCCCGAACCGCCTGGCTCTGCCGGCGCCCTCCTCAC1460.10995466252955972No Hit
TTACAGGGAAGTTCATGATCTTGAAAGGAAGTATGCTGTTCTCTATCAGCCT1430.10769532014881535No Hit
GAAACGGGTATTTGTACATAGTTTGTACATTTCACTGTGACAGGAGATAAAA1390.10468286364115617No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCGTTCTACACTGTCTCTTA1390.10468286364115617No Hit
TGCTCTGGGAGGTCAAAAAATACATAAATAAAACAAGTGAAGTAATACAACA1380.10392974951424139No Hit
ACAGAAGGGCAAATCTAAGTGAAAAACCTTTGTGTAAAACAGGTGAGTGGAG1370.10317663538732659No Hit
AAGCAAGGGGCGAGCAACCAATGTAGGATTGCCCACAGTTTTTCTTTTTAAA1360.10242352126041179No Hit
CATGGCGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1360.10242352126041179No Hit
CTCGAGGGGGCGGACCCCGGGCGGGCGCAGGGCGCGGGCTCCGGCGCCGCCG1360.10242352126041179No Hit
TGCAATGGGAAGTGAGATATTTCAAGTTAATACAGTGAGAAATAGAAAATAT1360.10242352126041179No Hit
TAGCAAGGGACCTGTAACTAGCTTTTTTAATTTATTATTTGAATTTTAGGAT1350.10167040713349701No Hit
AGCATGGGAATGTCGTCTCTTTCGTGGAAGATTGGGTGGTCTCATGTTGAGG1350.10167040713349701No Hit
AAGCTTGGGTCTCTAACTTCAATATCTGTTTGTCAGACTGCACTGCAGAATT1340.10091729300658221No Hit
ACCGGGGGGAGGCCGAACGTCAGGAGCGGGCGCGGGCCGAGGCGCAGGAGGC1340.10091729300658221No Hit
CGACGGGGTTACTCACCAATACACTTCCCAGTCATATAAAATTAGATACCTT1330.10016417887966741No Hit
TCTCACTGGGACAGCTTTGATTAGTTATTCATAATGAAAAAGGTATTCAGAG1330.10016417887966741No Hit
TGCAACGGGGCAAGGCCTCCTGTCTACAGCAGCTTCCTCAGTTTCCTACTGC1330.10016417887966741No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)