FastQCFastQC Report
Sat 31 Dec 2022
SRR5844926.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844926.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131091
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCGTTCTACATCTCGTATGC6760.5156723192286274No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTTCTACATCTCGTATGCCGTCT6550.49965291286205765Illumina PCR Primer Index 11 (95% over 21bp)
GCTTCTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1770.13502071080394534No Hit
GTGTCAGGGGTCTCTTTCAGCTAGAATTGAAATATCTATAAGACGAACTGGA1620.12357827768496694No Hit
GTGTGAGGGATCTTCCTCAAGGGATAGTACACACGCCTCAAGACGTTTTTAA1590.12128979106117124No Hit
AACGGCGGGTGAGAGTGTACTTTTACCAAAAGTTATCTAAGCCTGAGCACTT1570.11976413331197411No Hit
TAAACAGGAGAAGAAAGAAACCCATAGCTCAAAGACAAGGTCTTCAAATTAA1520.11594998893898131No Hit
CATGGCGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1490.11366150231518564No Hit
TTACAGGGAAGTTCATGATCTTGAAAGGAAGTATGCTGTTCTCTATCAGCCT1470.11213584456598852No Hit
GACTCAGGGATCAGACTGCATAAATGTATTTGTTTTAAGTAAATTATATTCA1450.11061018681679138No Hit
TCTGCGGGGATGGCTTGACTTGGAAGTTAGGGGATTTAATTTCCTTTCCCTA1430.10908452906759428No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTTCTACACTGTCTCTTATACAC1430.10908452906759428No Hit
TAGAGTGGGAAGCCGCTGCCGAAGAACTTCAGGAGGCTGCAGGCGCTGGGGA1430.10908452906759428No Hit
GTTGTAGGGGATGTATGTTAATTAAGGGGAAACCATGTGTATTCATGAAGGT1360.10374472694540435No Hit
AGCACCGGGAAAACCAAATGACTTAACACACCTTGACAACTGTTCATAAATT1360.10374472694540435No Hit
TCAAATGGGGACCCAGAAAGACATTTCTAGAAATCTTTTAGTAAGAAAATAC1330.10145624032160865No Hit
CTCGAGGGGGCGGACCCCGGGCGGGCGCAGGGCGCGGGCTCCGGCGCCGCCG1320.10069341144701008No Hit
GCCGCTGGGGCTGTTCCGGCGCACGTAATCGCCGAGGGCACGTGCATGCCCC1320.10069341144701008No Hit
AATGCAGGGCACATCTTTGCTCATGAGCCTGCCCACTTTCCCCCAATTGAGG1320.10069341144701008No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)