FastQCFastQC Report
Sat 31 Dec 2022
SRR5844925.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844925.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103194
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTTCTACATCTCGTATGCCGTCT6430.6230982421458612Illumina PCR Primer Index 11 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACCGTTCTACATCTCGTATGC6210.6017791732077447No Hit
TATAGAGGGTATTTTCTACCCCATATTTTACAGAAAAATGTATCACAGGTGG1790.17345969726922109No Hit
TGAAATGGGCATGCCCATCAGGGGTAGACTAGATAAAGAAAATGTAGTACGT1530.1482644339787197No Hit
GAGTAGGCTTTGCAGAGAATGAGTAGCCACTGCTGTTTGCCCTGGCCCATCA1520.1472953853906235No Hit
TGTGAAGGGGACAAACTGTGATCTGCCTTCACACCTTTAGTCAGTCTTTGAA1500.14535728821443106No Hit
ATCAGCGGGACAGGAAAGAATGAAGAAAAGAACAAAGTAAACTGTATGGAAA1480.14341919103823864No Hit
TTATATGGGAGGGCAGTAGCCCCAGGACTCCTAGTCGCCGGCTTCAGGTCAC1470.14245014245014245No Hit
GGGATGGAATAGGGTTCCTAACCTGACAACAATGAAAGAACCAAACTAACTT1440.13954299668585382No Hit
TTAAGAGGGGAGTGCTCAGGCAGGCGCTGCGGCAGAAGCACCAGGAAGCCCA1430.1385739480977576No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTTCTACACTGTCTCTTATACAC1430.1385739480977576No Hit
GATCGGCGGGATTTCAGTGAACGAATGTTCTTCAAGGAAACACCTAACAAAC1390.1346977537453728No Hit
GTTGCCGGGGAGGAGAAAGATGGCGGCTACCTTACTAGCTGCTCGGGGAGCC1390.1346977537453728No Hit
CCGCTGGGGATTGTCTTTTATAGCAAAAATAATGAGAAAAATAATATTAGCC1380.13372870515727658No Hit
TTAGCTGGGTATTGGATGTCATTGAGCCATCTGGGCTCATTCAGCAGGAAAT1350.13082155939298795No Hit
ACTCTTGGGTGATAAAGTAAATTATTAAAGTATTTTTTTCCCCAGTAGCCAA1350.13082155939298795No Hit
GTCAAAGGGATAATGAAACTATCTACAATTCTTGTTTTAGCACATCTGTTAT1350.13082155939298795No Hit
TCATTGCAGAAAGTCTGGAGTAAGTGACCACTGGGCTTTGGATGATCATCAT1330.12888346221679556No Hit
GAATGTGGGTAAAATAAGATTTTAAAGAATGTCATAATCATATTCAGAAAGT1310.12694536504060314No Hit
GTAGTAGGGGGGCACGCTGTGGGCAGGTGGGAGGGATAGAGGGGGGCTGCCT1310.12694536504060314No Hit
CATTGAGGGAACAAGGCATCAGTAAAACATGCATGAGTGAAGAGACCAGTAA1310.12694536504060314No Hit
GTTCCAGGGTGTAGTGATGTTTTCACAGTTGAAACAAACATATTGCTGTGCG1310.12694536504060314No Hit
GTTAGTGGGGTGTGGATGAGTATTGTGTAATATTTATTTTGCTTTATGAAAA1310.12694536504060314No Hit
CAGCGAAGGAAGGCATCAGCATATCTAAACAAAGATTGTGCCACCGAAGTGT1300.12597631645250693No Hit
CACATCGGGAGCCTCATAGTCTTCTACAAATGAAGGTATATTTGCAATATTG1300.12597631645250693No Hit
TTCAGCGGGGAATGACCAATCCCACTAAACATCTTTGAAGTCGGCCTAGAGA1300.12597631645250693No Hit
TCTCTAAGGGCGGTTGGAAACGCCAAGCCAGCTGCGTCCTAATCCAAAAGCC1290.12500726786441071No Hit
TACACTGGGATACTGACAGCAGATTGAACACTGACATTTAATTTCAGGGTTT1290.12500726786441071No Hit
TCACTGGGGCACTTCTTATTATATATCTAAAATTTTGATAGGCTGCATTTTC1270.12306917068821831No Hit
TTCAATGGGAAGTTTTTCAGATTTTCTTCAAAACTGTGTCTTTACAGTTGGA1270.12306917068821831No Hit
GGTTGGGTAATATCACAGGATCAATCATCTCAGAATCTTACTTGATGCATTA1260.1221001221001221No Hit
GAACTAGGGCTATATAAATATTGGGCTCAGCTATTCAAAACAATGTTTGAAT1240.12016202492392968No Hit
ATGGTTGGGATCTCTACTAAAAATACAAAATCAGCCAAGCGTGGTGGTGGGC1240.12016202492392968No Hit
GGCAATGGGTGCAGTGAAGGTGAGTGTTCTGTGCTAAGTGACAGCTGGGGCA1230.11919297633583349No Hit
GCTTGAGGGAATGTTTCATATGTCAGTACAGTTTCCACACCTATCTTTTCAA1230.11919297633583349No Hit
TTTGCTGGGTCATAGCAGGGAAGGATAGAATAAAATAATCCATTGTCCTGTG1220.11822392774773728No Hit
AATGACGGGCAAGTGTTCAGTAGTACAATAGGTAATCAATAATTGGCCTCTT1210.11725487915964106No Hit
CTATATGGGACTCAAGATGGCGGCTCCGGGCGGGCGTGGCCAGTGACTAGAA1210.11725487915964106No Hit
ACTCTTGGGTGGAATCAGCACATTTAGCCTTATTTATAAACCTCACTTCTGG1200.11628583057154485No Hit
GAATGGGGGCTGGAGTGCAATGGCGTGATCTCAGCTCACCGCAACCTCTGCC1200.11628583057154485No Hit
TGAAAGGGGGTACTTCCCTTGCCGCATAGTTTGAAAATGTCTCCAGGAAGAA1200.11628583057154485No Hit
ACCGCGGGGCCTCGCACCGTGCCCGGCTCTACCATGAAGATGGGCTCCCTGG1200.11628583057154485No Hit
GGCATAGGGAGAAATGGGCTAATGCTATGAATAATAATAACAAACACTGCCT1180.11434773339535245No Hit
ATTGCTGGGATTACAGGCGTGAGCCACCATGTTCGGCCAAAAAAACCCCTAT1180.11434773339535245No Hit
TACACTGGGCTTTAAAAATAGTATTTTTTTCTGATTATAAAGTAACACATTT1180.11434773339535245No Hit
GCATTAGGGTGCCACCTACTCATCTGACAAAGGGCTAATATCCAGAATCTAC1180.11434773339535245No Hit
GTGTTAGGGTAACAACATAGTTTGTTCATAAAAGCAATATTGAACTTTAGTT1170.11337868480725624No Hit
GCAAAAGGGTCCATGGGACTTATATTCTTATTCTAGAACTCTTCATTGCAGT1170.11337868480725624No Hit
CGCAAGGGGATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCT1170.11337868480725624No Hit
TATTACGGGACTCTCACAAACCAGGGTGAGCCGGGTGACAACGACATTGGGG1160.11240963621916003No Hit
CTGTTTGGGACCTCAGTTTGAGCTTTGTAAATAGGAATAAGGTTTGCCTTTT1160.11240963621916003No Hit
AAACAGGGGTGGTGGCTGCAGTGTATCCAGGATTACTGTAAAATGGTTCCAA1160.11240963621916003No Hit
TAAGCTGGGATTACACGTGTGAGCCACCACACCAGGCCCAAAGAAACATTTC1160.11240963621916003No Hit
TACACTGGGATCTTCCAGGAGCGAGATCCCTCCAAAATCAAGTGGGGCGATG1160.11240963621916003No Hit
ATTGTCGGGCTGGTAGCGGGCATAAGCCTGCGTCAGATTAAAACACTGAACT1160.11240963621916003No Hit
CAAACAGGGTATGCCTGTAAACTCTCTGAACAGAAAGAGCAGCTTGTTGAGA1150.11144058763106382No Hit
TGCGAAGGGCACAGAAGAAATAAGTGAGGCCATTGAAATGGATGAAGTCTTT1150.11144058763106382No Hit
GGGTTAAAAAAGCACCGTGGAGAATGGCCCTCTTCAGGAAAGAAAAATAAGC1150.11144058763106382No Hit
TGTGATGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGG1150.11144058763106382No Hit
GACCAAGGGCAGGGCTTTTTTTTTAAATTGAGTGTTATTGGACTTTGATGTT1150.11144058763106382No Hit
TTGAATGGGGACCTTTGAACTGGTATGGTTTGGCAGAATAGTTTTAAATTTT1150.11144058763106382No Hit
CACGATGGGGCAGCGTCAAGATTCAAACTCTGGCTTTCTTTTTTCTGAGTCT1140.11047153904296761No Hit
TTCCAGGGGGCGGGGAGGCGGCTGCCTTCGACCTCCGCCAGGAGAGTGGGAA1140.11047153904296761No Hit
CGATGTGGGGAGGTTGCAGTGAGCCAGCGTCGTGCCACTGCATGCCAGCCTG1140.11047153904296761No Hit
TTCAGCAGGGAGTTCAGCAGTGTTTTCTTATAGAAATTCTTTCTGTTCCATA1140.11047153904296761No Hit
GTGAAGGGGCTTAATTTTTCTACATCTGAATATATCCTTACACGTTTTTAAA1130.10950249045487141No Hit
AACAGGGAATTATCTCTGAGTAAAAAGCTTAACATAAACAAGATTTAGGGCT1130.10950249045487141No Hit
CAAGTAGGGGTGAAGACCAAGAAGGATGATTCTCACTCAGCAGAGGATAGTG1130.10950249045487141No Hit
TCGTATGGGCAAAAAATAAAATGTTTAATATATAAAAGCATGTTCTGAATAG1130.10950249045487141No Hit
AATAAAGGGGAATTAGAAATGGAAATGCCTGGCACAGCCAGGAAGGAGCTGC1130.10950249045487141No Hit
TCAGCAGGGTCTTATGTCTGGAAGATGTATCAAGAAAGGTGTTGGGAATTTT1130.10950249045487141No Hit
TTGCTGGGATCCGCTTCTGGCTTTTCTGGAAGTACCCCCAGGAGCAAGACAG1120.1085334418667752No Hit
CTACCAGGGCAAAACATTTTAAAAATTAGAAAATTTATAAGGTAAAATGTTA1120.1085334418667752No Hit
CCAGCTGGGTATCATATATAGTTAGGAAATAACCAGGCTTTGACTTGACCAA1120.1085334418667752No Hit
TGAACTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1120.1085334418667752No Hit
GTTGCCGGGGAATCAAAAATAGCCACTTTTTCCTTGGTCGTAGAGAGGTGTT1120.1085334418667752No Hit
GTTCCTGGGATGAGTATTCCCATTAATAGTCACTGATTGGAAATTATTCTGT1120.1085334418667752No Hit
TCATACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1120.1085334418667752No Hit
TGTCCGGGGTCTCCCAAGATATAAGAGGCAGCAGCAAACGTGCCTATTGACG1110.10756439327867899No Hit
GCTACAGGGACTTTCAGTCAAGTTTGAACTCTGTAAAACCTCATAAATAAGT1110.10756439327867899No Hit
AATTCAGGGTGATGCCAGGATTTTGAATGACTTGGGAGCTGGTTATTGCCGC1100.10659534469058278No Hit
TTCAACGGGAGATAATAAAGAAAAACAGCACTGGGCCTATAATTGGGAGTCT1100.10659534469058278No Hit
CTCGCAGGGTTGTGGTTAAGCGCATAAACTTTGGGGCCAGACTGGTTGGTTT1100.10659534469058278No Hit
GTAGAGGGGGAGAGTTGGCTGTATTTAGATTTATCACTTTTTAATAGGGTGA1100.10659534469058278No Hit
GGTTGCGGGATGAAATAGTTGAGATACACTGAATTATAAGCCAGAAATAATA1100.10659534469058278No Hit
AGACCCGGGAACCATAAATTAGCTTCACAAAAAGTTTGTGTACATAAAAGTG1090.10562629610248658No Hit
CGACTGGGGTGGTGATAGCTGCTGTCCAGTTGCCCCTCCATGGGCAGAAACC1090.10562629610248658No Hit
AAAGAGGGCATCTTTTGTAACAGGAGATAGCAGAGCATCTGAATAGTGATGC1090.10562629610248658No Hit
CACGACGGGAAGCTCACAAATAGTGATTATTTTCAAAAGATCTTTTTGTAAA1080.10465724751439037No Hit
TGTTCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1080.10465724751439037No Hit
TAGCCTCGGGGAACTTTTGGGAAGTCTAATTTATTGATTTCTTCACCTACAG1080.10465724751439037No Hit
TCCATCGGGCTCCACCCCAAATCAACAGAATATACATTCTTCTCAGCACCAC1080.10465724751439037No Hit
TCAACGGGGACGGCCGCGCAGCATCTGTCTTGCTGGAAGCTTTTTCCTAGAG1080.10465724751439037No Hit
ATATGAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1070.10368819892629416No Hit
CTATTTGAGGGGGAACTCGGCAAATCTTACCCCGCCTGTTTACCAAAAACAT1070.10368819892629416No Hit
TCGAAGGGGAGGGCTGGGTGAGGCAAGTTCTTAGTGAGGAGACTGCAGAGAC1070.10368819892629416No Hit
GGTTTGGGGGAAGGATGCCCTGTGTAGATTATTCAGTGCCACAAATTGAAAG1070.10368819892629416No Hit
CATAATTGGGATAACCTTAAGCCTCCAACTATTTCCGACTTTCACTAGAGGA1070.10368819892629416No Hit
AAAGGTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1070.10368819892629416No Hit
GTATTACGGGATTCATGCCATAAGTAAATATAGTGAAACTTAAAGCATTGAT1070.10368819892629416No Hit
TTAGTTGGGTGACAGAAGCGTCCCTGCGTTCTTCATGCTTGGACACAGCAAC1060.10271915033819795No Hit
AGCATTGGGTCATGCAGTCAAAGAAATGGTGAGAGCCTTGAAGATACACGAG1060.10271915033819795No Hit
CTGCAGGGTCCATATGAATTTTAAAGTAGTTTTTTCCAATTCTGTGAAGAAA1060.10271915033819795No Hit
TTTACAGGGGGGAAAGGCAGGAGGAATAAAAGATGTTTGGAACTTAAAAGAT1060.10271915033819795No Hit
GACTAAGGGTATTATTAAAGTAATAATGTGTTCCTTGAGGATAACTTGTCAA1060.10271915033819795No Hit
CTCCGCGGGATAGGATCTCATCGAGCCTCACGTCTACTCCCTTCTGCAGATG1050.10175010175010174No Hit
AAGACTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1050.10175010175010174No Hit
TATGGCTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG1050.10175010175010174No Hit
CGTCTGGGGTCTGGAGTGGGGACTGCACCGAGCGGCGGCAGCGGCAAGCTTG1050.10175010175010174No Hit
AATCGCGGACCGGTGGGACCAAAAGGGAACAGGTGCCTCAGCCATCCCAACA1050.10175010175010174No Hit
AGACTGGGGCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACA1050.10175010175010174No Hit
CCGGACAGGGCCGTGAGCTATGATTGTGCCACAGGTCTCCAGTCTGGGTGAC1040.10078105316200556No Hit
GAATGTGGGCCATGGAGTATTTGACAAGGTCATTGCATCAAATAGCTTCTTG1040.10078105316200556No Hit
GTTAGGGGGTTTTATCTATTTTTTGAGGTTTTTTTGTTTGCTTATTTTTCAA1040.10078105316200556No Hit
AGACAAGGGGGGTCTGCCAATCTCAAGATGTGCATTGTCAGCATGTGGGAAT1040.10078105316200556No Hit
CTAAAGGGGGTTGGTGGGAATGTAAATGAATATACCCATTATGAAAAACAGT1040.10078105316200556No Hit
ATCTCTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1040.10078105316200556No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCGTTCTACACTGTCTCTTA1040.10078105316200556No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)