FastQCFastQC Report
Sat 31 Dec 2022
SRR5844924.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844924.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102787
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCGTTCTACATCTCGTATGC6260.6090264333038224No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTTCTACATCTCGTATGCCGTCT6060.5895687197797387Illumina PCR Primer Index 11 (95% over 21bp)
TATAGAGGGTATTTTCTACCCCATATTTTACAGAAAAATGTATCACAGGTGG1850.179983850097775No Hit
GAGTAGGCTTTGCAGAGAATGAGTAGCCACTGCTGTTTGCCCTGGCCCATCA1610.15663459386887446No Hit
TACACTGGGATCTTCCAGGAGCGAGATCCCTCCAAAATCAAGTGGGGCGATG1560.15177016548785352No Hit
TGAAATGGGCATGCCCATCAGGGGTAGACTAGATAAAGAAAATGTAGTACGT1560.15177016548785352No Hit
GTTAGTGGGGTGTGGATGAGTATTGTGTAATATTTATTTTGCTTTATGAAAA1550.15079727981164934No Hit
ATCAGCGGGACAGGAAAGAATGAAGAAAAGAACAAAGTAAACTGTATGGAAA1460.1420413087258116No Hit
TCTCTAAGGGCGGTTGGAAACGCCAAGCCAGCTGCGTCCTAATCCAAAAGCC1420.13814976602099488No Hit
ATGGTTGGGATCTCTACTAAAAATACAAAATCAGCCAAGCGTGGTGGTGGGC1420.13814976602099488No Hit
CCGCTGGGGATTGTCTTTTATAGCAAAAATAATGAGAAAAATAATATTAGCC1420.13814976602099488No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTTCTACACTGTCTCTTATACAC1380.13425822331617812No Hit
TTAAGCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG1380.13425822331617812No Hit
CGTCTGGGGTCTGGAGTGGGGACTGCACCGAGCGGCGGCAGCGGCAAGCTTG1370.1332853376399739No Hit
TTTGCTGGGTCATAGCAGGGAAGGATAGAATAAAATAATCCATTGTCCTGTG1370.1332853376399739No Hit
GTTGCCGGGGAATCAAAAATAGCCACTTTTTCCTTGGTCGTAGAGAGGTGTT1350.13133956628756555No Hit
GGGATGGAATAGGGTTCCTAACCTGACAACAATGAAAGAACCAAACTAACTT1330.12939379493515718No Hit
TTAAGAGGGGAGTGCTCAGGCAGGCGCTGCGGCAGAAGCACCAGGAAGCCCA1320.12842090925895297No Hit
CATTGAGGGAACAAGGCATCAGTAAAACATGCATGAGTGAAGAGACCAGTAA1320.12842090925895297No Hit
GATCGGCGGGATTTCAGTGAACGAATGTTCTTCAAGGAAACACCTAACAAAC1300.1264751379065446No Hit
GAATGTGGGTAAAATAAGATTTTAAAGAATGTCATAATCATATTCAGAAAGT1300.1264751379065446No Hit
GCTTGAGGGAATGTTTCATATGTCAGTACAGTTTCCACACCTATCTTTTCAA1300.1264751379065446No Hit
GAACTAGGGCTATATAAATATTGGGCTCAGCTATTCAAAACAATGTTTGAAT1270.12355648087793204No Hit
GCAAAAGGGTCCATGGGACTTATATTCTTATTCTAGAACTCTTCATTGCAGT1260.12258359520172783No Hit
TTCAGCAGGGAGTTCAGCAGTGTTTTCTTATAGAAATTCTTTCTGTTCCATA1260.12258359520172783No Hit
TTAGCTGGGTATTGGATGTCATTGAGCCATCTGGGCTCATTCAGCAGGAAAT1250.12161070952552365No Hit
ATTGTCGGGCTGGTAGCGGGCATAAGCCTGCGTCAGATTAAAACACTGAACT1240.12063782384931947No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCGTTCTACACTGTCTCTTA1240.12063782384931947No Hit
AGAATAGGGAGACCAACCCACATCAAGAGACATTCTGCAAAACAACAACCAG1230.11966493817311527No Hit
CCGATAGGGAAGGAATTTGTTGGTCAAATAATGACAATGCTTAACAAGGGCT1230.11966493817311527No Hit
TTCAGCGGGGAATGACCAATCCCACTAAACATCTTTGAAGTCGGCCTAGAGA1230.11966493817311527No Hit
TACACTGGGATACTGACAGCAGATTGAACACTGACATTTAATTTCAGGGTTT1220.11869205249691109No Hit
CACATCGGGAGCCTCATAGTCTTCTACAAATGAAGGTATATTTGCAATATTG1220.11869205249691109No Hit
GTCAAAGGGATAATGAAACTATCTACAATTCTTGTTTTAGCACATCTGTTAT1220.11869205249691109No Hit
ACTCTTGGGTGGAATCAGCACATTTAGCCTTATTTATAAACCTCACTTCTGG1210.1177191668207069No Hit
TATTACGGGACTCTCACAAACCAGGGTGAGCCGGGTGACAACGACATTGGGG1210.1177191668207069No Hit
GGGTTAAAAAAGCACCGTGGAGAATGGCCCTCTTCAGGAAAGAAAAATAAGC1210.1177191668207069No Hit
TTGCTGGGATCCGCTTCTGGCTTTTCTGGAAGTACCCCCAGGAGCAAGACAG1200.11674628114450271No Hit
GTACATGGGGGAGGTTGATGGGGGTGGATCACTTGAGGTCAGGAGTTTGAGA1200.11674628114450271No Hit
CATAAAGGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGG1200.11674628114450271No Hit
TGTGAAGGGGACAAACTGTGATCTGCCTTCACACCTTTAGTCAGTCTTTGAA1200.11674628114450271No Hit
CGATGTGGGGAGGTTGCAGTGAGCCAGCGTCGTGCCACTGCATGCCAGCCTG1200.11674628114450271No Hit
CAAACTGGGACTAAACGCAGACGAAAATGGAAAGATTAATTGGGAGTGGTAG1200.11674628114450271No Hit
ACCGCGGGGCCTCGCACCGTGCCCGGCTCTACCATGAAGATGGGCTCCCTGG1190.11577339546829853No Hit
TCTACTGGGATGTTCTCTGAATTTCTTACAACTTTATTATTATTGATACTAT1180.11480050979209434No Hit
CGACTGGGGTGGTGATAGCTGCTGTCCAGTTGCCCCTCCATGGGCAGAAACC1180.11480050979209434No Hit
TAAGCTGGGATTACACGTGTGAGCCACCACACCAGGCCCAAAGAAACATTTC1180.11480050979209434No Hit
AATTCAGGGTGATGCCAGGATTTTGAATGACTTGGGAGCTGGTTATTGCCGC1170.11382762411589013No Hit
ACTCTTGGGTGATAAAGTAAATTATTAAAGTATTTTTTTCCCCAGTAGCCAA1170.11382762411589013No Hit
CCAGCTGGGTATCATATATAGTTAGGAAATAACCAGGCTTTGACTTGACCAA1170.11382762411589013No Hit
TCCATCGGGCTCCACCCCAAATCAACAGAATATACATTCTTCTCAGCACCAC1170.11382762411589013No Hit
TCATTGCAGAAAGTCTGGAGTAAGTGACCACTGGGCTTTGGATGATCATCAT1170.11382762411589013No Hit
GGCACAGGGGTCAGCTTCCCAACAATACTTTGTGTCTTTTGTTCTCTCTTGG1170.11382762411589013No Hit
CAAACAGGGTATGCCTGTAAACTCTCTGAACAGAAAGAGCAGCTTGTTGAGA1160.11285473843968595No Hit
AAAGGTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1160.11285473843968595No Hit
GTATTACGGGATTCATGCCATAAGTAAATATAGTGAAACTTAAAGCATTGAT1160.11285473843968595No Hit
AACAGGGAATTATCTCTGAGTAAAAAGCTTAACATAAACAAGATTTAGGGCT1150.11188185276348177No Hit
GTTGCCGGGGAGGAGAAAGATGGCGGCTACCTTACTAGCTGCTCGGGGAGCC1150.11188185276348177No Hit
GAATGGGGGCTGGAGTGCAATGGCGTGATCTCAGCTCACCGCAACCTCTGCC1140.11090896708727757No Hit
CTATTTGAGGGGGAACTCGGCAAATCTTACCCCGCCTGTTTACCAAAAACAT1140.11090896708727757No Hit
CCGGACAGGGCCGTGAGCTATGATTGTGCCACAGGTCTCCAGTCTGGGTGAC1140.11090896708727757No Hit
GGCATAGGGAGAAATGGGCTAATGCTATGAATAATAATAACAAACACTGCCT1140.11090896708727757No Hit
GTTCCAGGGTGTAGTGATGTTTTCACAGTTGAAACAAACATATTGCTGTGCG1140.11090896708727757No Hit
CTAACTGGGGGGGGGGCGGAACGCCGCCCGGCCGCGGGTCGGGCTCTGTGTC1130.10993608141107339No Hit
TTTAAAGGGCAGTAATATTCTTTCTTTTTAAATTTTCTTAAACTTATACATT1130.10993608141107339No Hit
TGTTCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1130.10993608141107339No Hit
CGATATGGGAAGGTATTGAATAAGGTATTCTTGTCAGTTTAGAAATGGACTG1130.10993608141107339No Hit
TATGGCTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG1130.10993608141107339No Hit
GGTTTGGGGGAAGGATGCCCTGTGTAGATTATTCAGTGCCACAAATTGAAAG1130.10993608141107339No Hit
AATAAAGGGGAATTAGAAATGGAAATGCCTGGCACAGCCAGGAAGGAGCTGC1130.10993608141107339No Hit
GTTAATGGGATAATCAAGCTAATTAATATCTTCATTACCTCTACATAATTAC1130.10993608141107339No Hit
GCATTAGGGTGCCACCTACTCATCTGACAAAGGGCTAATATCCAGAATCTAC1130.10993608141107339No Hit
TCATACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1120.1089631957348692No Hit
TATGATGGGAGCTCCCAGATTCGTCAGAATTATTCCACCGACGTGGAGGCAG1120.1089631957348692No Hit
GCATCGCGGGTATGGGGGAGTGCCATCAATAAATAAAAATAATACCATTGCC1110.107990310058665No Hit
CACGATGGGGCAGCGTCAAGATTCAAACTCTGGCTTTCTTTTTTCTGAGTCT1110.107990310058665No Hit
TACGGAAGGGCACCCTGCAAGACTGGGATAGAGGGCAATGGATATTCCCACA1110.107990310058665No Hit
GCAATAGGGATTCCTCAGGTCATTCAGGTGGTCATGGCCCAGCCAGGCTTTG1110.107990310058665No Hit
GCTACAGGGACTTTCAGTCAAGTTTGAACTCTGTAAAACCTCATAAATAAGT1110.107990310058665No Hit
CAGCGAAGGAAGGCATCAGCATATCTAAACAAAGATTGTGCCACCGAAGTGT1110.107990310058665No Hit
GATCTGGGGGGACTAACAGACATGTCCAACTTTCTCTCCAGTTCTTAGCTCA1110.107990310058665No Hit
CTACCAGGGCAAAACATTTTAAAAATTAGAAAATTTATAAGGTAAAATGTTA1100.10701742438246081No Hit
ACGCTTGGGAAGAGGTTATTGATAAACTTCTGAGGCAGACATTTGTCTCGCT1100.10701742438246081No Hit
TACACTGGGCTTTAAAAATAGTATTTTTTTCTGATTATAAAGTAACACATTT1100.10701742438246081No Hit
GGCAATGGGTGCAGTGAAGGTGAGTGTTCTGTGCTAAGTGACAGCTGGGGCA1100.10701742438246081No Hit
GTGAAGGGGCTTAATTTTTCTACATCTGAATATATCCTTACACGTTTTTAAA1090.10604453870625664No Hit
AGTTGCGGGATATTACTATTGTTATTGTTTCCCTGAATAATTGAACTGAATT1090.10604453870625664No Hit
GTAGTAGGGGGGCACGCTGTGGGCAGGTGGGAGGGATAGAGGGGGGCTGCCT1090.10604453870625664No Hit
AACCCCGGGTATCCCCATTGGCCACTGGTGGAGGTGCACTGGAAGGTCAGGG1090.10604453870625664No Hit
GGTTGCGGGATGAAATAGTTGAGATACACTGAATTATAAGCCAGAAATAATA1090.10604453870625664No Hit
TGCCTCGGGCAGTGGCTTGTAGTAATGTTTTTTATTTATATATCATTTTTTC1090.10604453870625664No Hit
GCTTGTCTTCCTTTCTGATTCTTCATGTAGTTCTTCCTGTGTTTCTTAGCAT1080.10507165303005243No Hit
TTATATGGGAGGGCAGTAGCCCCAGGACTCCTAGTCGCCGGCTTCAGGTCAC1080.10507165303005243No Hit
GTAGTGGGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGG1080.10507165303005243No Hit
TCGAAGGGGAGGGCTGGGTGAGGCAAGTTCTTAGTGAGGAGACTGCAGAGAC1080.10507165303005243No Hit
AAGCGTAGGGAGACTGGGTAATTCACTGATAATGAGTCATTACCTTTTTAAT1080.10507165303005243No Hit
AATCGCGGACCGGTGGGACCAAAAGGGAACAGGTGCCTCAGCCATCCCAACA1080.10507165303005243No Hit
GACTCAGGGTGTAGTTGCAGCTGATGCCTGAATGTATCCTAGCTGACAAATT1070.10409876735384825No Hit
TAGCAGGGGGGGCGACCGCGGCGGCCTGACGAGCCGGGCCGGGCGGGCCGGC1070.10409876735384825No Hit
GAATCCGGGTGTGTTTCCTTTGGCTGTTCCCTAATACAAAACACGGACATAT1070.10409876735384825No Hit
TTTACAGGGGGGAAAGGCAGGAGGAATAAAAGATGTTTGGAACTTAAAAGAT1070.10409876735384825No Hit
TCTCAGGGGATTGGATCTGAGCTTGTACAGAAATACGTCGGTGAGGGGGCTC1070.10409876735384825No Hit
TACTGAGGGAATTAATGTGAGGAAGCTCCTCCAATCCTCTGGCTATTTAAGG1070.10409876735384825No Hit
GACATAAGGGGCCTAAAATTGGGCAAAATCATCTAACATTAAGCCTATTTCA1070.10409876735384825No Hit
ACGCCTGGGATGCTTCTTTATACTTTACCCACATGACAGGACATACCCTCAA1060.10312588167764405No Hit
CTCGCAGGGTTGTGGTTAAGCGCATAAACTTTGGGGCCAGACTGGTTGGTTT1060.10312588167764405No Hit
TTCATTGGGATTGTTGAAAAGCCCAGAGTGATCTATAGATTCAGTGCGATCT1050.10215299600143987No Hit
GGTTGGGTAATATCACAGGATCAATCATCTCAGAATCTTACTTGATGCATTA1050.10215299600143987No Hit
CTATATGGGCATGAGAGATGATGTGGCAACACAGTTTGGCCAGATGACCCTG1050.10215299600143987No Hit
GTTCCTGGGATGAGTATTCCCATTAATAGTCACTGATTGGAAATTATTCTGT1050.10215299600143987No Hit
CGCTCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1040.10118011032523569No Hit
AAGTATGGGGATAAATTGCTAAAGTACTAGGGAGCTTGTAAATCTTAAAAAT1040.10118011032523569No Hit
GTAACAAGGGTCACTTGCTATAAAGGTAATTGTAAAAATAAATACAGTGGAC1040.10118011032523569No Hit
AATGTAGGGGGGTGGAAAAAGATATTCCAGGCAAATGGACACCAAAAGCAAG1040.10118011032523569No Hit
TGTGATGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGG1040.10118011032523569No Hit
AGACAAGGGGGGTCTGCCAATCTCAAGATGTGCATTGTCAGCATGTGGGAAT1040.10118011032523569No Hit
AATGACGGGCAAGTGTTCAGTAGTACAATAGGTAATCAATAATTGGCCTCTT1040.10118011032523569No Hit
GCCTATGGGCCGATCTGCGTAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGC1040.10118011032523569No Hit
CCTACAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1040.10118011032523569No Hit
TCAGCAGGGTCTTATGTCTGGAAGATGTATCAAGAAAGGTGTTGGGAATTTT1040.10118011032523569No Hit
ACTAAAGGGTTTATCTCTAAATATAGGTTAGCTGTAATTATTTCATGAATTT1030.10020722464903148No Hit
TATAGAGGGATTCTAATGGTAGCTTCCTGTAGTTTTAAGGTACTGCATATTT1030.10020722464903148No Hit
AAGACGGGTAGATATCCAAAGCTAAGTTCAGCTTCTTTGTTCAAATTCAAGC1030.10020722464903148No Hit
TAAAACGGGCGATCTTATAGAGGGTGGTGCCCACCTTTCGAGGCATTGCGAG1030.10020722464903148No Hit
TCGTATGGGCAAAAAATAAAATGTTTAATATATAAAAGCATGTTCTGAATAG1030.10020722464903148No Hit
CGCAAGGGGATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCT1030.10020722464903148No Hit
TGAAAGGGGGTACTTCCCTTGCCGCATAGTTTGAAAATGTCTCCAGGAAGAA1030.10020722464903148No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)