FastQCFastQC Report
Sat 31 Dec 2022
SRR5844923.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844923.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102300
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTTCTACATCTCGTATGCCGTCT6210.6070381231671554Illumina PCR Primer Index 11 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACCGTTCTACATCTCGTATGC5710.5581622678396873No Hit
ATGGTTGGGATCTCTACTAAAAATACAAAATCAGCCAAGCGTGGTGGTGGGC1480.14467253176930597No Hit
TTCAGCGGGGAATGACCAATCCCACTAAACATCTTTGAAGTCGGCCTAGAGA1480.14467253176930597No Hit
TCTCTAAGGGCGGTTGGAAACGCCAAGCCAGCTGCGTCCTAATCCAAAAGCC1470.1436950146627566No Hit
TATAGAGGGTATTTTCTACCCCATATTTTACAGAAAAATGTATCACAGGTGG1450.14173998044965788No Hit
ATTGTCGGGCTGGTAGCGGGCATAAGCCTGCGTCAGATTAAAACACTGAACT1430.13978494623655913No Hit
GAACTAGGGCTATATAAATATTGGGCTCAGCTATTCAAAACAATGTTTGAAT1410.1378299120234604No Hit
ATCAGCGGGACAGGAAAGAATGAAGAAAAGAACAAAGTAAACTGTATGGAAA1400.13685239491691104No Hit
CATTGAGGGAACAAGGCATCAGTAAAACATGCATGAGTGAAGAGACCAGTAA1390.13587487781036167No Hit
CAAACTGGGACTAAACGCAGACGAAAATGGAAAGATTAATTGGGAGTGGTAG1390.13587487781036167No Hit
GTCAAAGGGATAATGAAACTATCTACAATTCTTGTTTTAGCACATCTGTTAT1380.1348973607038123No Hit
TTCAGCAGGGAGTTCAGCAGTGTTTTCTTATAGAAATTCTTTCTGTTCCATA1360.13294232649071358No Hit
GGGATGGAATAGGGTTCCTAACCTGACAACAATGAAAGAACCAAACTAACTT1350.13196480938416422No Hit
GATCGGCGGGATTTCAGTGAACGAATGTTCTTCAAGGAAACACCTAACAAAC1330.1300097751710655No Hit
TTAGCTGGGTATTGGATGTCATTGAGCCATCTGGGCTCATTCAGCAGGAAAT1320.12903225806451613No Hit
GTAGTAGGGGGGCACGCTGTGGGCAGGTGGGAGGGATAGAGGGGGGCTGCCT1320.12903225806451613No Hit
GAGTAGGCTTTGCAGAGAATGAGTAGCCACTGCTGTTTGCCCTGGCCCATCA1300.1270772238514174No Hit
TGAAATGGGCATGCCCATCAGGGGTAGACTAGATAAAGAAAATGTAGTACGT1300.1270772238514174No Hit
TACACTGGGATCTTCCAGGAGCGAGATCCCTCCAAAATCAAGTGGGGCGATG1290.12609970674486803No Hit
GTTAGTGGGGTGTGGATGAGTATTGTGTAATATTTATTTTGCTTTATGAAAA1290.12609970674486803No Hit
AATAAAGGGGAATTAGAAATGGAAATGCCTGGCACAGCCAGGAAGGAGCTGC1280.12512218963831867No Hit
GAATGTGGGTAAAATAAGATTTTAAAGAATGTCATAATCATATTCAGAAAGT1270.1241446725317693No Hit
GTTGCCGGGGAGGAGAAAGATGGCGGCTACCTTACTAGCTGCTCGGGGAGCC1270.1241446725317693No Hit
CCGCTGGGGATTGTCTTTTATAGCAAAAATAATGAGAAAAATAATATTAGCC1270.1241446725317693No Hit
TATTACGGGACTCTCACAAACCAGGGTGAGCCGGGTGACAACGACATTGGGG1260.12316715542521994No Hit
TCATTGCAGAAAGTCTGGAGTAAGTGACCACTGGGCTTTGGATGATCATCAT1250.12218963831867058No Hit
GTTCCAGGGTGTAGTGATGTTTTCACAGTTGAAACAAACATATTGCTGTGCG1250.12218963831867058No Hit
ACTCTTGGGTGGAATCAGCACATTTAGCCTTATTTATAAACCTCACTTCTGG1240.12121212121212122No Hit
AGTTGCGGGATATTACTATTGTTATTGTTTCCCTGAATAATTGAACTGAATT1240.12121212121212122No Hit
GATCTGGGGGGACTAACAGACATGTCCAACTTTCTCTCCAGTTCTTAGCTCA1240.12121212121212122No Hit
CTATATGGGACTCAAGATGGCGGCTCCGGGCGGGCGTGGCCAGTGACTAGAA1230.12023460410557185No Hit
ACTCTTGGGTGATAAAGTAAATTATTAAAGTATTTTTTTCCCCAGTAGCCAA1220.11925708699902249No Hit
AGGCCTGGAGTTTTGTTTTGTTTTGTATAAAGATTTTAACTGCAAATACAAT1220.11925708699902249No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTTCTACACTGTCTCTTATACAC1210.11827956989247312No Hit
TTTGCTGGGTCATAGCAGGGAAGGATAGAATAAAATAATCCATTGTCCTGTG1210.11827956989247312No Hit
ACAAATGGTGAGCTCCCTTAGTGAATTTTGTCTCCTGCTTGGAAAGTCTCTC1210.11827956989247312No Hit
AAGCGTAGGGAGACTGGGTAATTCACTGATAATGAGTCATTACCTTTTTAAT1210.11827956989247312No Hit
GCAAAAGGGTCCATGGGACTTATATTCTTATTCTAGAACTCTTCATTGCAGT1200.11730205278592376No Hit
TACACTGGGATACTGACAGCAGATTGAACACTGACATTTAATTTCAGGGTTT1200.11730205278592376No Hit
GTTCCTGGGATGAGTATTCCCATTAATAGTCACTGATTGGAAATTATTCTGT1190.11632453567937438No Hit
GGCATAGGGAGAAATGGGCTAATGCTATGAATAATAATAACAAACACTGCCT1180.11534701857282502No Hit
TTTACAGGGGGGAAAGGCAGGAGGAATAAAAGATGTTTGGAACTTAAAAGAT1180.11534701857282502No Hit
ACGCTTGGGAAGAGGTTATTGATAAACTTCTGAGGCAGACATTTGTCTCGCT1180.11534701857282502No Hit
AATCGCGGACCGGTGGGACCAAAAGGGAACAGGTGCCTCAGCCATCCCAACA1180.11534701857282502No Hit
GGTTGGGTAATATCACAGGATCAATCATCTCAGAATCTTACTTGATGCATTA1170.11436950146627566No Hit
GAATGGGGGCTGGAGTGCAATGGCGTGATCTCAGCTCACCGCAACCTCTGCC1160.11339198435972629No Hit
CAGCGAAGGAAGGCATCAGCATATCTAAACAAAGATTGTGCCACCGAAGTGT1160.11339198435972629No Hit
AATGACGGGCAAGTGTTCAGTAGTACAATAGGTAATCAATAATTGGCCTCTT1160.11339198435972629No Hit
GTTGCCGGGGAATCAAAAATAGCCACTTTTTCCTTGGTCGTAGAGAGGTGTT1160.11339198435972629No Hit
CTCCTGGGATAACATTTCTTCTTTCAACAGAGTATATGTTTCCCATTTAACC1150.11241446725317693No Hit
TTAAGAGGGGAGTGCTCAGGCAGGCGCTGCGGCAGAAGCACCAGGAAGCCCA1150.11241446725317693No Hit
AACAGGGAATTATCTCTGAGTAAAAAGCTTAACATAAACAAGATTTAGGGCT1150.11241446725317693No Hit
AAGACTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1150.11241446725317693No Hit
TGAAAGGGGGTACTTCCCTTGCCGCATAGTTTGAAAATGTCTCCAGGAAGAA1150.11241446725317693No Hit
CACGATGGGGCAGCGTCAAGATTCAAACTCTGGCTTTCTTTTTTCTGAGTCT1140.11143695014662756No Hit
AAATATGGGGAGGACCTTTGTACTGCTAAATCCAGTAGCCTATTGTCAGTTC1140.11143695014662756No Hit
TGTGAAGGGGACAAACTGTGATCTGCCTTCACACCTTTAGTCAGTCTTTGAA1140.11143695014662756No Hit
TACACTGGGCTTTAAAAATAGTATTTTTTTCTGATTATAAAGTAACACATTT1140.11143695014662756No Hit
ACTAAAGGGTTTATCTCTAAATATAGGTTAGCTGTAATTATTTCATGAATTT1130.1104594330400782No Hit
CAAGTAGGGGTGAAGACCAAGAAGGATGATTCTCACTCAGCAGAGGATAGTG1130.1104594330400782No Hit
TTTTCAGGGACTGCTGCTGCCACTAATATCATTATCTTTTAATTTTTAAGCA1130.1104594330400782No Hit
AGCTGATGATCTGGATGATAAAACTTGAGAAAATGTCCCAGGAAACAAAAAG1130.1104594330400782No Hit
TCTACTGGGATGTTCTCTGAATTTCTTACAACTTTATTATTATTGATACTAT1130.1104594330400782No Hit
GTAACAAGGGTCACTTGCTATAAAGGTAATTGTAAAAATAAATACAGTGGAC1130.1104594330400782No Hit
GCAATAGGGAGTTAGAAATGTGATAATGGTTGTTTTAAAATCTTTACCTCTG1120.10948191593352884No Hit
GTTAGGGGGTTTTATCTATTTTTTGAGGTTTTTTTGTTTGCTTATTTTTCAA1120.10948191593352884No Hit
CGTCTGGGGTCTGGAGTGGGGACTGCACCGAGCGGCGGCAGCGGCAAGCTTG1120.10948191593352884No Hit
AGCATTGGGTCATGCAGTCAAAGAAATGGTGAGAGCCTTGAAGATACACGAG1120.10948191593352884No Hit
TGTCCGGGGTCTCCCAAGATATAAGAGGCAGCAGCAAACGTGCCTATTGACG1110.10850439882697947No Hit
TTAAGCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG1110.10850439882697947No Hit
CACATCGGGAGCCTCATAGTCTTCTACAAATGAAGGTATATTTGCAATATTG1110.10850439882697947No Hit
ATTGCTGGGATTACAGGCGTGAGCCACCATGTTCGGCCAAAAAAACCCCTAT1110.10850439882697947No Hit
CTCCGCGGGATAGGATCTCATCGAGCCTCACGTCTACTCCCTTCTGCAGATG1100.10752688172043011No Hit
TCTTTAGGGGAGTCACATGACACGTTTCCCGTCAAGATGGCGGATACTCTCC1100.10752688172043011No Hit
CGCAAGGGGATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCT1100.10752688172043011No Hit
AATGCAGGGCATAAATGTTTTTAATGACAATATTCTGTTGAACGGTTGTACC1100.10752688172043011No Hit
GTCTGAGGGATTGATGAAAACATCTAATCACTATCTATTAACTTTCCTGTGA1090.10654936461388075No Hit
TCACTGGGGCACTTCTTATTATATATCTAAAATTTTGATAGGCTGCATTTTC1090.10654936461388075No Hit
TGAACTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1090.10654936461388075No Hit
AGACAAGGGGGGTCTGCCAATCTCAAGATGTGCATTGTCAGCATGTGGGAAT1090.10654936461388075No Hit
TGACGAGGGAATAAAGCATTGGCTAAATCTTAAAGAATCTCAGGAAGAACAG1090.10654936461388075No Hit
TTGCTGGGATCCGCTTCTGGCTTTTCTGGAAGTACCCCCAGGAGCAAGACAG1080.10557184750733138No Hit
CTGCAGGGTCCATATGAATTTTAAAGTAGTTTTTTCCAATTCTGTGAAGAAA1080.10557184750733138No Hit
TGCCTCGGGCAGTGGCTTGTAGTAATGTTTTTTATTTATATATCATTTTTTC1080.10557184750733138No Hit
TTCAGCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1070.10459433040078202No Hit
CCGGACAGGGCCGTGAGCTATGATTGTGCCACAGGTCTCCAGTCTGGGTGAC1070.10459433040078202No Hit
GTAGAGGGGGAGAGTTGGCTGTATTTAGATTTATCACTTTTTAATAGGGTGA1070.10459433040078202No Hit
GCTGTTGGGGCTTAAACATAAACACGCAATTATAGAATTTTTTCCCTGCAAA1070.10459433040078202No Hit
GTCGCGGGTTATCTGATCTTCACGTTTCTTGGAAGCAGAATCATCAAGGCTT1070.10459433040078202No Hit
GCTTGAGGGAATGTTTCATATGTCAGTACAGTTTCCACACCTATCTTTTCAA1070.10459433040078202No Hit
TATGATGGGAGCTCCCAGATTCGTCAGAATTATTCCACCGACGTGGAGGCAG1070.10459433040078202No Hit
CACATAGGGGACGCTCTTCCGATCTATAACGGGGCCCTCCTCCCAGTCGCGC1060.10361681329423265No Hit
TTCAACGGGAGATAATAAAGAAAAACAGCACTGGGCCTATAATTGGGAGTCT1060.10361681329423265No Hit
AGAATAGGGAGACCAACCCACATCAAGAGACATTCTGCAAAACAACAACCAG1060.10361681329423265No Hit
AAAGGTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1060.10361681329423265No Hit
TTTAAGGGGCACCACACTGAGCCCCCACTTGCGTGTTCTTGAAAGCAGTATG1060.10361681329423265No Hit
AAGTTTGGGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAAT1050.10263929618768329No Hit
GTACATGGGGGAGGTTGATGGGGGTGGATCACTTGAGGTCAGGAGTTTGAGA1050.10263929618768329No Hit
CTCGCAGGGTTGTGGTTAAGCGCATAAACTTTGGGGCCAGACTGGTTGGTTT1050.10263929618768329No Hit
TGGTCAGGGTCTGGGTTTATTCCAGCTTTTTATCTTGACGTGTCCCATTCAC1050.10263929618768329No Hit
TAGCAGCGGGGAAGTGTTGAATGTGATACAAGGTAAAGTACATGTCACAAGG1050.10263929618768329No Hit
TAAGGCGGGGTTAGTGGTGGCTTATACCTATTTATATTTGTATTAATTGCTT1050.10263929618768329No Hit
GGCAATGGGTGCAGTGAAGGTGAGTGTTCTGTGCTAAGTGACAGCTGGGGCA1050.10263929618768329No Hit
GCATTAGGGTGCCACCTACTCATCTGACAAAGGGCTAATATCCAGAATCTAC1050.10263929618768329No Hit
CTACCAGGGCAAAACATTTTAAAAATTAGAAAATTTATAAGGTAAAATGTTA1040.10166177908113391No Hit
TACGGAAGGGCACCCTGCAAGACTGGGATAGAGGGCAATGGATATTCCCACA1040.10166177908113391No Hit
GGGCGGGGGCTAAATAAACGGGTTCAGATGCAGCCTGGAAAAGCCTGTGTAA1040.10166177908113391No Hit
CATGCAGGGAGGACCAATCTGTGCAACATGGTGAGACCCCTGTCTCTATTAA1040.10166177908113391No Hit
CGATGTGGGGAGGTTGCAGTGAGCCAGCGTCGTGCCACTGCATGCCAGCCTG1040.10166177908113391No Hit
ATCTCTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1040.10166177908113391No Hit
AATTCAGGGTGATGCCAGGATTTTGAATGACTTGGGAGCTGGTTATTGCCGC1030.10068426197458455No Hit
CACTAAGGGCACCTTTTGGGGTCCCCAGTGTTGTTTCCTTCCTTATGTTCAT1030.10068426197458455No Hit
GCTTGTCTTCCTTTCTGATTCTTCATGTAGTTCTTCCTGTGTTTCTTAGCAT1030.10068426197458455No Hit
TATAACGGGGTTTATAGTGAGGTAAATGTCCTAAGGGCTTGGAAATTTAAAG1030.10068426197458455No Hit
CTTAAGGGGCACTAAGTACGTTTTTAGGCTCCTCTCAAATACTGCGTATTGA1030.10068426197458455No Hit
TCGTATGGGCAAAAAATAAAATGTTTAATATATAAAAGCATGTTCTGAATAG1030.10068426197458455No Hit
TAAGCTGGGATTACACGTGTGAGCCACCACACCAGGCCCAAAGAAACATTTC1030.10068426197458455No Hit
AAAGAGGGCATCTTTTGTAACAGGAGATAGCAGAGCATCTGAATAGTGATGC1030.10068426197458455No Hit
ACCGCGGGGCCTCGCACCGTGCCCGGCTCTACCATGAAGATGGGCTCCCTGG1030.10068426197458455No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)