FastQCFastQC Report
Sat 31 Dec 2022
SRR5844919.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844919.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107767
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTTCTACATCTCGTATGCCGTCT8940.8295674928317573Illumina PCR Primer Index 11 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACCGTTCTACATCTCGTATGC8270.7673963272615922No Hit
ATGCTAGGGGGGCGTCTAGAGGCTATTAAGGAAACAACTGTTCATTGGATGA1800.16702701197954847No Hit
ACCGGGGTGGCTGACCACGTGGCCGAGGAGCTCCAGCATCTGCTGCAGTGGC1690.1568198056919094No Hit
CTTGGTGGGGTATCCTGAGACTTTGGTGAAGTTGCTTATCAGCTTAAGGAGA1670.15496395000324775No Hit
TGACGCGGGATTGCTGGAAAGACCGTCCCAAAATTAGAGGTGCAATTAGTCA1660.15403602215891693No Hit
CTAATGGTGGGAAGGGAGAAAGGAGAGAGAAAGAAAAGAGAAAGAAAGAGAA1640.15218016647025526No Hit
TCAACCGGGACTGAGACTTGTGTATTTAATTTGGAAATGAGACTTCTGAGAC1640.15218016647025526No Hit
CTTAGCGGGAGAGGTTCAGAATTGGAAGTACAATTTCATTGCTTTTGCAATT1610.1493963829372628No Hit
CTGCAGGGGACTCGCGCAGACCTAGCGCGTCCAGGTGGGAGGTTAGTGTGGC1550.14382881587127783No Hit
GACCTGGGGGTGCCAAAAACACACAATGGAAGAAGGAAATCCTCTTCAATAA1510.14011710449395454No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTTCTACACTGTCTCTTATACAC1510.14011710449395454No Hit
AATTATGGGAATGGAACTGTGGTACACCCCAGACTTTGTATTAACAAGTTCC1480.13733332096096207No Hit
AGTTAAGGGATTCCTTTGTAACTTGCTGGCATCATAAACTTGGATCAGGTTT1480.13733332096096207No Hit
CTGCTTGGGAAGGGTGAGTCTCACAGAGCTTTTAAGTTTGGCCTCTCACACT1450.1345495374279696No Hit
ATATCAGGGGAGATGTTAGATTTTTATCAAAAGTTTTTTCTGCATCTGGTGA1440.13362160958363878No Hit
CTCAGCCGGGCATTTAAATAAACTGTCATCTTGTTGTTGGTGATGTTTTCTG1440.13362160958363878No Hit
TTGTCCGGCTGCTGAAAGAATAGAGTCTTAAACTTTAATAATCACAGCCTTG1440.13362160958363878No Hit
AGGCCTGGGGAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCG1430.13269368173930796No Hit
CAATACGGGACATATCTTGTGCTGTGATATTAAAGTAGTATTCTGTTCTGGT1420.1317657538949771No Hit
CTCAGTGGGTGGCTACCTAATTGGTGTCTTTATGTTTTAGATGTTTTTGTGG1410.1308378260506463No Hit
TTCTTGGGGAGGATATAGAAGACTTAGATCACTACGAGATGAAAGCAGAGCC1410.1308378260506463No Hit
ATATCCGGGGAGGAGGCGGGGCGCCTGTGGGAGCCGTGGAGGGAACTTTCCC1400.1299098982063155No Hit
ACAGCAGGGATAATATAAAATTTTCATCTGAAAGTTTGTAGGAGTTTACATT1400.1299098982063155No Hit
AAGTCAGGGGATCCCCGTCATCATGGAATGGTGCCTGGAGAGGAGGCTTCTG1400.1299098982063155No Hit
TGTGCGTGGGGGGGAGCAAAGAGGGCCTCAGAGAAGTCCTGACTCATAAAAA1380.12805404251765382No Hit
CAAAAAGGGGTATTTCAGTTTTGTCTTTTGATCTGGCATTGTACCAGAAATT1380.12805404251765382No Hit
GTATAGCAGGGAAACGACAAAAATTATTACCCAGGATGCTTAAAGATCTTCT1380.12805404251765382No Hit
GACAATGGGATATCAGAATGTTTCTATGTGCCACTTCCAAGGCATTTATCTG1370.127126114673323No Hit
ACTGGGGGGATACTGGGCAGTTCGAGACAGGTTTTTAATCATAAGTGGTCTT1370.127126114673323No Hit
GTACACGGGAAGAATGATGGTGGCTATTTCATTTTGGGGTATGGCTGTTGGT1360.1261981868289922No Hit
TATAATGGGAACTAACTTGTGAGTTGATTATGTATCCTGCAACTTTACCGAA1360.1261981868289922No Hit
GTACCTGGGAACCTTTTAAAGTGTAAAATCTTTGGATTTACAGTCACTGGTT1360.1261981868289922No Hit
CAAGCTGGGGTCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAA1360.1261981868289922No Hit
CAAGACGGGGGGCACCCGAGGCTCAGTAGAGGTTTAGTATGATGCCTTCCCT1350.12527025898466135No Hit
AGCGCAGGGATATGTAAACTAACGTGACATATAAAATGGATAAATACTTTAA1340.12434233114033053No Hit
GCCATAGGGTCATCTTGACTAAAATTATTTGAGCATTCATATTTTTTCCAAA1330.1234144032959997No Hit
GCCTAGGGGAAGTGAATATCGAGTTGTAGCACTGGATTTGAGAGGTTATGGA1330.1234144032959997No Hit
CATCCAGGGATGCCAGTATCTGCCAGCAGAATGGCATTGTGCCCATCGTGGA1330.1234144032959997No Hit
CCATTAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1310.12155854760733806No Hit
GCGTTTGGGCACTTTTTCTCTCTACTCCCACCCACACCTGCCTCCCTCTTAC1310.12155854760733806No Hit
CAAGATGGGTGTCAACAACTTTGTTGTTGGGAGTGAAGAAGGTTCTGTGTAC1310.12155854760733806No Hit
GTGCAGGGGAGGGTTCTGTATAAATAATAAATTACAATAACGATGGAGTGTC1300.12063061976300724No Hit
ATGCTTGGGACGAGCATATTTCACCTCCGCTACCATAATCATCGCTATCCCC1300.12063061976300724No Hit
CAAGTAGGGGAAGGGAAGGGCTAGAAGTTCTCATGAGGCTCCTCAAGAAGTT1300.12063061976300724No Hit
AGTTTGGGCTTCCGATCTAAGCCTGGGACTATTTTGAATTCATTCATTTCAT1290.1197026919186764No Hit
GCTAGTGGGGGTTTGAGAAATGGGAAGATGAATATTTACTCTGTAAATATGA1290.1197026919186764No Hit
TTGTTGGGGCATGTGTCACCCATCTTTTCATATCCATTTCTATGGTATTTGG1290.1197026919186764No Hit
TGTAAGGGGTGCAGTTATGGTTATAGCACAAGGCAATTGCCTGATGTAAAAG1280.11877476407434558No Hit
GTGAAAGGGCAAGAAAGGCTTGAAAGAAGTGAAAATAAACAGCCACGGGTGA1280.11877476407434558No Hit
TTGCAGGGGGATTCTCAGCCCTCGGTCAAAAGAATCTGTTCCAGAATTCCCC1280.11877476407434558No Hit
GACAGTGGGTCTTAACTTTTGTCTTTGAAAACATCTGTATCCATGATCTCTA1280.11877476407434558No Hit
CGATGGGGGAATCAAAGACATTTCATCCACCAATATCATGTGTAGATATTAT1270.11784683623001475No Hit
ATTTGGGGGATTGGGACAGACCTAGTTCAATGAATCTGAGGAGGCTACTCAG1270.11784683623001475No Hit
TATTAAGGGTATCGGTCTTGGGAGATCAACCCTGAACCTGGAATGTGCTGGG1270.11784683623001475No Hit
TTTTGTGGGGGATTCCAGTGGCAATGAAACACATTTTACTGGGAACGAAGTT1270.11784683623001475No Hit
TGGCCTGGCTGGCCCTGAGGGCTGAGAAGGAGAGGCTGCAGCAGCAAAAGCG1260.11691890838568395No Hit
CTCATTCGGGAACGTGGTGAAACCCTGTCTCTACTAAAAATACAAAAATTGT1250.1159909805413531No Hit
GACGTGGGATTCGCTTTCCATTTTTCAATGATTGCTCTCTTTCATTGCCTGA1250.1159909805413531No Hit
AAGCCTGGGCAGCGGAGTGAGACTCTGTCTCAAGGAAAGAAAGAAAAAGAAA1240.11506305269702229No Hit
AGACGGGGGAAAGAATCTGTACTGCAAGTAAAACCTACTCCCCAAAAATGTG1240.11506305269702229No Hit
TAACCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1240.11506305269702229No Hit
CTCGACGGGTAACTGCATGATTTCTGTTTTATCTACCTCTAAAGCAAATCTG1240.11506305269702229No Hit
ACGTTTGGGGACAGCTTGGTGCTGGTAAGAGGAAGCCCGTGGTTCTGGCTAG1240.11506305269702229No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCGTTCTACACTGTCTCTTA1240.11506305269702229No Hit
ATTAGAGGGATGCTTCTAGTGGGCTGCTTCTGGCTACACCCCCAGATCCCTG1230.11413512485269146No Hit
TGCTCAGGGCCCTCTTTATTTCTATTTGCCTTAGTTTTGCATCTCCCCACCT1230.11413512485269146No Hit
TATTTCGGGACAACCCATCCTTCCACTTGGAATATTTTTACCACCTATCCTG1230.11413512485269146No Hit
TACAATGGGGGAGTTTTAAAAAAATCTTTGTGACCTTGAATAAACAAAGGTT1230.11413512485269146No Hit
TTGTACGGGGGCTCTCGTCTGGACTTTTGAACTCCTGGCCACCATTCAACTC1230.11413512485269146No Hit
TAAGCTGGGATCACCATAATATCCACATCTCAGGTTCCACCCACTTGGCATA1220.11320719700836063No Hit
GGACCGGATGGGCCATGGAGCCTTCCTGCAGCACGTGGTGCTGGCGGCCTGC1220.11320719700836063No Hit
GTACACGCGGGATACTTATTTTACTTTCTAGACCTAGGCTAGATGTTTTAAG1220.11320719700836063No Hit
GCGATTGGGCATTGGCACCCAGATACCAATAACAGACAAAGACATAACAAGA1220.11320719700836063No Hit
AGTGACGGGAACCTTTTATTAATTCAACTTTTCTTTACTTATAAAGTGATCT1210.1122792691640298No Hit
AAATGTGGGAACCCAATGAGAACCAGATCTCTGGTGGAGAGCCATCCTCTAC1210.1122792691640298No Hit
CTGCTAGGGGTGTGTGGATGGATGAATGAGCAGTGGGAGTGCAGCGCCAGGT1210.1122792691640298No Hit
TGAACTGGGGGACCTCTCCCTGATTACATAAGCTAAGGGGTCACATATGGCT1200.11135134131969898No Hit
TAGACGTGGGAATAACATCAAATGTAAATGGACTAAACTCTTCAATAAAAGA1190.11042341347536815No Hit
TTAGCTGGGTTATAGTAGCATAGTTACAACAACCATTCAATGCTTTGCAGTC1190.11042341347536815No Hit
TTGTGGGGGGGTTTTGCATTCTAAGACAAGAGTAATATTGTTTTAGACTGTG1190.11042341347536815No Hit
TTTTAGAGGGAGGGGCAGAACAGATAGATTACGACAGGTTTGGTTTTTAAAT1190.11042341347536815No Hit
GCTGCCATGGGTGAGTTTGTGTTCGTGAATGCCCTGGTGACAAGAGTCTCTT1180.10949548563103732No Hit
GCAACCGGGATTCCTAATCCTGAAGTAGAGAGACTTAATACACACTTGTAGC1180.10949548563103732No Hit
TATGTAGGGGAAACAGTTAGCTTCAGTTAATTGCTAAGGATGTGGCAGAAGA1180.10949548563103732No Hit
TTAGTCGGGGAAGATCACCAGTGGCATATCCAGGCATGCTGTGCTCTAACTG1180.10949548563103732No Hit
AAAGTAGGGGTGGTTTAATATGGCTGTATGACTGGGGAAGTGGGAGTGGTAA1170.10856755778670651No Hit
ACACACGGGAAAGTCTAAATGAAGAAGCTGCCTGAGATTGTGGCTTTCAGGC1170.10856755778670651No Hit
TGCCAAGGGGCCCTGTTAAGGGGCAGGTTTCTCTTTAGCCCTCTTCCTGCAC1170.10856755778670651No Hit
GCGCTAGGGGAGGCAAACACCGAGCTCCTCCAGAGCCGCCCGGGAGCAGGGA1160.10763962994237566No Hit
GTTGGCGGGATATTAATGTTGAGAGAGAGGAATGGAAGTTGGATACACTTTG1160.10763962994237566No Hit
TAGTTTGGGCTAACTTCCCAGGTGACAGTAGCAGGAACAAGGAAGGGTAATG1160.10763962994237566No Hit
CGATTCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1160.10763962994237566No Hit
GGTAATGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1160.10763962994237566No Hit
TCCCAGGGAAGGAGAAGCAGCTCTTGCTCGGTTGGGAGACGGTGCAAGAGAA1140.10578377425371403No Hit
AAATATGGGGGTATCATCAAGCTCAACGTTCTGGAGTGGCTGCAGCTCACCA1140.10578377425371403No Hit
AACCTAGGGGAATATAATGAAATATATTTTGAACTTAAATTATATTCTATAT1140.10578377425371403No Hit
ACTCCAGGGATCCTCATGTAAGAGAGGATTCTCCAGTCATGTGATTTGCTCA1140.10578377425371403No Hit
AAACCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1140.10578377425371403No Hit
TTGTCTGGGGATCTAAATTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCT1140.10578377425371403No Hit
GTTAGTGGGCAGCGTTGTTGCGGGAAGTCAGGGACCCTGAACCGAGGAGCCA1140.10578377425371403No Hit
ATTTGTGGGACATCACACCTGTGACGGAAGCACTTCTGGAAGTGAACACTCG1140.10578377425371403No Hit
GATTCAGGGTTTGTTACTGAAGAAGACAAGAGGATTCTTCGTGACATTGAGA1130.10485584640938321No Hit
AACAGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1130.10485584640938321No Hit
CAGTGGGGGATAGTGAACATAATTTTGTGCTCACATTTGTTATTCCAAAGGC1130.10485584640938321No Hit
CTTGTTGGGCAAATCAGATCTGGATCTATAATGAAGAGCATGAATTGGTTTT1130.10485584640938321No Hit
TCCTATGGGGGGATACTTCATGGTGAGAAACTTGAAGATTTTCTCACACTAT1130.10485584640938321No Hit
GATACAGGGGTATTCATTCCCTTTAAAATGAAACATTCCTGGTTTATTTGTC1130.10485584640938321No Hit
CTAGTCGGGGGTTCTTTGTAGATGCATGTGTAAGAAGCTATTTTATAGCTTG1120.10392791856505237No Hit
TTGAAAGGGACGGTGGCAGAGGGAGGAAGTAACTCATACTAGGAAGAACAAA1120.10392791856505237No Hit
TCTCATGGGTGTGCATCATGCTGTGGTGAATAACCTTGTTGGCACCTCACTG1120.10392791856505237No Hit
GTCGACGGGCAGAAAAGTTAAAAGAATAGTACCATGAATACCCACATAGCTT1120.10392791856505237No Hit
GCCACTGGGAGACATTCCCTATTTAAGAACCATTTGGTTACAGTGGGTTAAG1120.10392791856505237No Hit
TCTGCACGGGTACACCTTCCTCATGAAAATATTTTTTAAAAGAATGGAAGAA1120.10392791856505237No Hit
GCATAAGGGGAAATACGATGCATCCAAATCAGAAAACAAGGAGTAAAATTCT1120.10392791856505237No Hit
TAGCCTGGGATGAAGATCCCTTGCAATGACTCTAAAATCACCAGTGCTGTTT1110.10299999072072155No Hit
TTCGATGGGGTGCTGGGCACTTTTGTGTTGTGGCAATGGCATGAGTTTGCAA1110.10299999072072155No Hit
AGTTGTGGGATACTTTATCACTTAACATGATATTGGCATTTTTGCCACTAAA1110.10299999072072155No Hit
AGAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGC1110.10299999072072155No Hit
TGTATAGGGTCACCTCTCAAATAAAAACATGCTGAAAAACTAGGACAGCAAA1110.10299999072072155No Hit
GAAACTGGGATCCTATTGATTTGTAATCAATCAGGTTGTTTTCAGGATTTTT1110.10299999072072155No Hit
CTACAACAGGGTCCCCAAAATATCTACTAGCCAGCCAATTTAACATCAACCG1110.10299999072072155No Hit
AGTACCGGGACTGCATGTAAATGTTCTGAACTAAAGGTAGATAGCAATATGT1110.10299999072072155No Hit
AACTAAGGGTTACTGAAAACCAACTGACTACTGATGTCTCATGTTGGTGTAT1110.10299999072072155No Hit
TTCGCGGGGATTCTACACCCTTTACCTTCCTAGATAATTATGTCTAAGTAGT1100.10207206287639074No Hit
GATTATGGGAATGTGCCCTTTCTTTTCTGTCTTTCTTTCCTTCTGTCTTTCT1100.10207206287639074No Hit
GTTGTAGGGGATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGAT1100.10207206287639074No Hit
TTTACTGGGGTAAATGGTGCCTAAAAGATATTTGAAGAATAAGTGACCAGCA1100.10207206287639074No Hit
CTCCACGGGAAGGGAGAGAGATTGCTTATTGATGATATAAATTTAAAAATTG1100.10207206287639074No Hit
CTCGTAGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1100.10207206287639074No Hit
TGCGCTGGGTACTAAAGAAAATTTCCCAGTAGAACTTTAAAAGCTGTTTTTC1100.10207206287639074No Hit
CTAGAACGGGGAGGGGTATGTGACTGTAACAAATGATGATGAAATGAAAGTG1100.10207206287639074No Hit
CAGCAAGGGCCACTTGGGAGGGCAGAAGCCAGGAATTCCACACCCTTGTGTT1100.10207206287639074No Hit
GTTTCAGGGATCTCGGGTATTTCTGAGCATATAAGAACTTTGAGAAGAGTTC1090.1011441350320599No Hit
CCACATGGGTGCGAGGGGGGTGCTCGCTTCGGCAGCACATATACTAAAATTG1090.1011441350320599No Hit
CTACAGGGGAAGCGCACAAACGGAAAGAGCGGGCGCGGTGCGCAGGGGCGGG1090.1011441350320599No Hit
GTCGCAGGATTCTTCTGGTGTTATCCTTCAGGATTGTTCCACTAAAATTTAT1080.10021620718772908No Hit
ATACAAGGGAGATTGATGACTTCTGTGAATAGAAATCATCCTGATCCTATTT1080.10021620718772908No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)