FastQCFastQC Report
Sat 31 Dec 2022
SRR5844918.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844918.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107330
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTTCTACATCTCGTATGCCGTCT8930.8320134165657319Illumina PCR Primer Index 11 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACCGTTCTACATCTCGTATGC7720.7192769961800056No Hit
ATGCTAGGGGGGCGTCTAGAGGCTATTAAGGAAACAACTGTTCATTGGATGA1900.17702413118419827No Hit
CTTGGTGGGGTATCCTGAGACTTTGGTGAAGTTGCTTATCAGCTTAAGGAGA1790.16677536569458679No Hit
AGCGCAGGGATATGTAAACTAACGTGACATATAAAATGGATAAATACTTTAA1710.15932171806577844No Hit
TCAACCGGGACTGAGACTTGTGTATTTAATTTGGAAATGAGACTTCTGAGAC1650.1537314823441722No Hit
ACCGGGGTGGCTGACCACGTGGCCGAGGAGCTCCAGCATCTGCTGCAGTGGC1650.1537314823441722No Hit
CTGCAGGGGACTCGCGCAGACCTAGCGCGTCCAGGTGGGAGGTTAGTGTGGC1640.15279977639057113No Hit
TGACGCGGGATTGCTGGAAAGACCGTCCCAAAATTAGAGGTGCAATTAGTCA1620.15093636448336906No Hit
TTGTCCGGCTGCTGAAAGAATAGAGTCTTAAACTTTAATAATCACAGCCTTG1560.14534612876176278No Hit
CTCGACGGGTAACTGCATGATTTCTGTTTTATCTACCTCTAAAGCAAATCTG1550.14441442280816175No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTTCTACACTGTCTCTTATACAC1530.14255101090095965No Hit
AGTTAAGGGATTCCTTTGTAACTTGCTGGCATCATAAACTTGGATCAGGTTT1500.1397558930401565No Hit
TTAGTCGGGGAAGATCACCAGTGGCATATCCAGGCATGCTGTGCTCTAACTG1500.1397558930401565No Hit
AAGTCAGGGGATCCCCGTCATCATGGAATGGTGCCTGGAGAGGAGGCTTCTG1490.1388241870865555No Hit
TATAATGGGAACTAACTTGTGAGTTGATTATGTATCCTGCAACTTTACCGAA1480.13789248113295444No Hit
TAACCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1480.13789248113295444No Hit
CTAATGGTGGGAAGGGAGAAAGGAGAGAGAAAGAAAAGAGAAAGAAAGAGAA1450.1350973632721513No Hit
CAAGTAGGGGAAGGGAAGGGCTAGAAGTTCTCATGAGGCTCCTCAAGAAGTT1450.1350973632721513No Hit
AGTGCTGGGATTACAGGCGTGAGCCACTGCACCCAGCCAAAAATTTTACATC1440.13416565731855026No Hit
CAAGACGGGGGGCACCCGAGGCTCAGTAGAGGTTTAGTATGATGCCTTCCCT1440.13416565731855026No Hit
ACAGCAGGGATAATATAAAATTTTCATCTGAAAGTTTGTAGGAGTTTACATT1430.13323395136494923No Hit
TCTGCACGGGTACACCTTCCTCATGAAAATATTTTTTAAAAGAATGGAAGAA1410.13137053945774713No Hit
AACGCAGGGAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGT1410.13137053945774713No Hit
CATCCAGGGATGCCAGTATCTGCCAGCAGAATGGCATTGTGCCCATCGTGGA1410.13137053945774713No Hit
TAGACGTGGGAATAACATCAAATGTAAATGGACTAAACTCTTCAATAAAAGA1390.12950712755054505No Hit
ACCATGGGGGTAGTACTGGTTCTAACAAGTAACAAGCAAGTTTTAAAATTTT1380.12857542159694402No Hit
TCCTATGGGGGGATACTTCATGGTGAGAAACTTGAAGATTTTCTCACACTAT1380.12857542159694402No Hit
GGTAATGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1380.12857542159694402No Hit
TAAGCTGGGATCACCATAATATCCACATCTCAGGTTCCACCCACTTGGCATA1370.12764371564334295No Hit
GGACCGGATGGGCCATGGAGCCTTCCTGCAGCACGTGGTGCTGGCGGCCTGC1370.12764371564334295No Hit
GACAATGGGATATCAGAATGTTTCTATGTGCCACTTCCAAGGCATTTATCTG1350.12578030373614088No Hit
TTGCAGGGGGATTCTCAGCCCTCGGTCAAAAGAATCTGTTCCAGAATTCCCC1350.12578030373614088No Hit
TTGTTGGGGCATGTGTCACCCATCTTTTCATATCCATTTCTATGGTATTTGG1350.12578030373614088No Hit
GTATAGCAGGGAAACGACAAAAATTATTACCCAGGATGCTTAAAGATCTTCT1350.12578030373614088No Hit
GTGAAAGGGCAAGAAAGGCTTGAAAGAAGTGAAAATAAACAGCCACGGGTGA1340.12484859778253983No Hit
GCAACCGGGATTCCTAATCCTGAAGTAGAGAGACTTAATACACACTTGTAGC1340.12484859778253983No Hit
CTCATTCGGGAACGTGGTGAAACCCTGTCTCTACTAAAAATACAAAAATTGT1330.12391689182893878No Hit
TGCTCAGGGCCCTCTTTATTTCTATTTGCCTTAGTTTTGCATCTCCCCACCT1330.12391689182893878No Hit
GACCTGGGGGTGCCAAAAACACACAATGGAAGAAGGAAATCCTCTTCAATAA1330.12391689182893878No Hit
TTAGCTGGGTTATAGTAGCATAGTTACAACAACCATTCAATGCTTTGCAGTC1330.12391689182893878No Hit
TATGTAGGGGAAACAGTTAGCTTCAGTTAATTGCTAAGGATGTGGCAGAAGA1330.12391689182893878No Hit
AAAGTAGGGGTGGTTTAATATGGCTGTATGACTGGGGAAGTGGGAGTGGTAA1320.12298518587533773No Hit
CTCAGTGGGTGGCTACCTAATTGGTGTCTTTATGTTTTAGATGTTTTTGTGG1320.12298518587533773No Hit
TGGCCTGGCTGGCCCTGAGGGCTGAGAAGGAGAGGCTGCAGCAGCAAAAGCG1320.12298518587533773No Hit
AGGCCTGGGGAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCG1310.12205347992173671No Hit
GTACACGGGAAGAATGATGGTGGCTATTTCATTTTGGGGTATGGCTGTTGGT1310.12205347992173671No Hit
ATATCAGGGGAGATGTTAGATTTTTATCAAAAGTTTTTTCTGCATCTGGTGA1310.12205347992173671No Hit
AATTATGGGAATGGAACTGTGGTACACCCCAGACTTTGTATTAACAAGTTCC1300.12112177396813567No Hit
AAAATGGGGTAACCAGGTGCATATGTGGTTTTACACATGATGATGGATACAT1300.12112177396813567No Hit
AGTTGTGGGATACTTTATCACTTAACATGATATTGGCATTTTTGCCACTAAA1300.12112177396813567No Hit
CAAGATGGGTGTCAACAACTTTGTTGTTGGGAGTGAAGAAGGTTCTGTGTAC1300.12112177396813567No Hit
CGCAAGGGTAAGTATATAAAATGAGTGTCGTGAAATCCTGTGCATTTACAAG1290.12019006801453462No Hit
ACACACGGGAAAGTCTAAATGAAGAAGCTGCCTGAGATTGTGGCTTTCAGGC1290.12019006801453462No Hit
CAGTGGGGGATAGTGAACATAATTTTGTGCTCACATTTGTTATTCCAAAGGC1290.12019006801453462No Hit
CCTGACCTCGTGATCCGCCCGCCTCGGCCTCCCAAAGTGCTGGGATTACAGG1290.12019006801453462No Hit
GTACCTGGGAACCTTTTAAAGTGTAAAATCTTTGGATTTACAGTCACTGGTT1290.12019006801453462No Hit
GCCATAGGGTCATCTTGACTAAAATTATTTGAGCATTCATATTTTTTCCAAA1280.11925836206093357No Hit
CTTGTTGGGCAAATCAGATCTGGATCTATAATGAAGAGCATGAATTGGTTTT1280.11925836206093357No Hit
TGCCAAGGGGCCCTGTTAAGGGGCAGGTTTCTCTTTAGCCCTCTTCCTGCAC1280.11925836206093357No Hit
TATTAAGGGTATCGGTCTTGGGAGATCAACCCTGAACCTGGAATGTGCTGGG1280.11925836206093357No Hit
TGAACTGGGGGACCTCTCCCTGATTACATAAGCTAAGGGGTCACATATGGCT1270.11832665610733252No Hit
GTTGGCGGGATATTAATGTTGAGAGAGAGGAATGGAAGTTGGATACACTTTG1260.11739495015373148No Hit
ATTAGAGGGATGCTTCTAGTGGGCTGCTTCTGGCTACACCCCCAGATCCCTG1260.11739495015373148No Hit
TTTAGCGGGATTTAGTGACAGATCAAATGCATACTTGAACTAAGATTGGCTT1260.11739495015373148No Hit
TTCTTGGGGAGGATATAGAAGACTTAGATCACTACGAGATGAAAGCAGAGCC1260.11739495015373148No Hit
TGTGCGTGGGGGGGAGCAAAGAGGGCCTCAGAGAAGTCCTGACTCATAAAAA1250.11646324420013043No Hit
AACCCTGGGGTTCAGTGACTTCTATATTTGGAAAGCAACATAGACTTAATAA1250.11646324420013043No Hit
ACTCCAGGGATCCTCATGTAAGAGAGGATTCTCCAGTCATGTGATTTGCTCA1250.11646324420013043No Hit
CTGCTTGGGAAGGGTGAGTCTCACAGAGCTTTTAAGTTTGGCCTCTCACACT1250.11646324420013043No Hit
TCGCTTGGGGGAGAGCGGGCCGAGGAGATTGGCGACGGTGTCGCCCGTGTTT1250.11646324420013043No Hit
TGTAAGGGGTGCAGTTATGGTTATAGCACAAGGCAATTGCCTGATGTAAAAG1240.11553153824652938No Hit
AGTGACGGGAACCTTTTATTAATTCAACTTTTCTTTACTTATAAAGTGATCT1240.11553153824652938No Hit
AGTTTGGGCTTCCGATCTAAGCCTGGGACTATTTTGAATTCATTCATTTCAT1240.11553153824652938No Hit
CAATACGGGACATATCTTGTGCTGTGATATTAAAGTAGTATTCTGTTCTGGT1230.11459983229292835No Hit
ACTGGGGGGATACTGGGCAGTTCGAGACAGGTTTTTAATCATAAGTGGTCTT1230.11459983229292835No Hit
CAGCAAGGGCCACTTGGGAGGGCAGAAGCCAGGAATTCCACACCCTTGTGTT1230.11459983229292835No Hit
AACCTAGGGGAATATAATGAAATATATTTTGAACTTAAATTATATTCTATAT1220.11366812633932732No Hit
TACAATGGGGGAGTTTTAAAAAAATCTTTGTGACCTTGAATAAACAAAGGTT1220.11366812633932732No Hit
TTGTGGGGGGGTTTTGCATTCTAAGACAAGAGTAATATTGTTTTAGACTGTG1220.11366812633932732No Hit
CGGTTTGGGAATAAGGCTACATCTGAAGACTAAAATAGCCTAAGCTCTTGAG1210.11273642038572627No Hit
CTTAGCGGGAGAGGTTCAGAATTGGAAGTACAATTTCATTGCTTTTGCAATT1210.11273642038572627No Hit
CTCTATGGGGGTTCCTGGCCTGACCCCCACCAAGGCCCATACCGCAGTAGGC1210.11273642038572627No Hit
GATTATGGGAATGTGCCCTTTCTTTTCTGTCTTTCTTTCCTTCTGTCTTTCT1200.11180471443212522No Hit
ATGTATGGGGACCGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAGGAG1200.11180471443212522No Hit
TTTTAGAGGGAGGGGCAGAACAGATAGATTACGACAGGTTTGGTTTTTAAAT1200.11180471443212522No Hit
TTTTGTGGGGGATTCCAGTGGCAATGAAACACATTTTACTGGGAACGAAGTT1200.11180471443212522No Hit
CTAGTCGGGGGTTCTTTGTAGATGCATGTGTAAGAAGCTATTTTATAGCTTG1190.11087300847852419No Hit
CCATTAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1190.11087300847852419No Hit
AAAAATGGGAGCGACAGAAATCCGTACTTGCCTGCACTTTGAGGATAATTGA1190.11087300847852419No Hit
CGTGGTGGGATTAAAATTCAAGAAATACATATTTAAGCATTTGTACAAAGAT1190.11087300847852419No Hit
AGCTGGGGCTTTCCCTTTTCTTACCTTCCTACTCACGTTTCCCTGCTTCTGG1190.11087300847852419No Hit
ATTTGTGGGACATCACACCTGTGACGGAAGCACTTCTGGAAGTGAACACTCG1190.11087300847852419No Hit
TAGCCTGGGATGAAGATCCCTTGCAATGACTCTAAAATCACCAGTGCTGTTT1180.10994130252492314No Hit
CTCCAAGGGTGGTAGATCACTTGAGGTCAAGAGTTGTGACACCAGCCTGGCC1180.10994130252492314No Hit
ATAGAAGGGGGCAGTTGTTGACGTTCTTGTTCATCTTTTCAGTCACCCTCCT1180.10994130252492314No Hit
GTACACGCGGGATACTTATTTTACTTTCTAGACCTAGGCTAGATGTTTTAAG1180.10994130252492314No Hit
CTCATTGGGGCTTTTTCCCATCTTGCAAGATGGCGGGTGAAAAAGTTGAGAA1180.10994130252492314No Hit
GATTCAGGGTTTGTTACTGAAGAAGACAAGAGGATTCTTCGTGACATTGAGA1170.10900959657132209No Hit
AAGCGAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1170.10900959657132209No Hit
TATCAGGGCTTCTATCTAGAATATATAAAGAATGCTTACAACTTCATACTAA1170.10900959657132209No Hit
GCTGCCATGGGTGAGTTTGTGTTCGTGAATGCCCTGGTGACAAGAGTCTCTT1160.10807789061772104No Hit
GCGCTAGGGGAGGCAAACACCGAGCTCCTCCAGAGCCGCCCGGGAGCAGGGA1160.10807789061772104No Hit
GTGCAGGGGAGGGTTCTGTATAAATAATAAATTACAATAACGATGGAGTGTC1160.10807789061772104No Hit
TTGTCTGGGGATCTAAATTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCT1160.10807789061772104No Hit
TGCGCTGGGTACTAAAGAAAATTTCCCAGTAGAACTTTAAAAGCTGTTTTTC1160.10807789061772104No Hit
ACGGAGGGGCTTTTGGCTCTCTGACCAGCACCATGGCGGTTGGCAAGAACAA1160.10807789061772104No Hit
ATACAAGGGAGATTGATGACTTCTGTGAATAGAAATCATCCTGATCCTATTT1160.10807789061772104No Hit
GCGTTTGGGCACTTTTTCTCTCTACTCCCACCCACACCTGCCTCCCTCTTAC1150.10714618466412No Hit
GAAACTGGGATCCTATTGATTTGTAATCAATCAGGTTGTTTTCAGGATTTTT1150.10714618466412No Hit
CAGTCCGGGAGGCGTAGGCCATGACCATTTGGGGTACGAGAGCCTAATTTTG1150.10714618466412No Hit
TCCCAGGGAAGGAGAAGCAGCTCTTGCTCGGTTGGGAGACGGTGCAAGAGAA1140.10621447871051895No Hit
CCACATGGGTGCGAGGGGGGTGCTCGCTTCGGCAGCACATATACTAAAATTG1140.10621447871051895No Hit
GCAAATGGGCACATTTGTAATCTTCATTTTTAACTGTACATTTGCTTAAATG1140.10621447871051895No Hit
AGGCTAGGGGGAGGGATTTCGGCCTGAGAGCGGGCCGAGGAGATTGGCGACG1130.10528277275691793No Hit
AAAAGTGGGATTAGGTCCAAGTTCTAACAATGACATTGAAAGAAAAACTAAT1130.10528277275691793No Hit
AACAGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1130.10528277275691793No Hit
AGAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGC1130.10528277275691793No Hit
CTCCACGGGAAGGGAGAGAGATTGCTTATTGATGATATAAATTTAAAAATTG1130.10528277275691793No Hit
TCCTAAGGGCTATAATTTTTGTACCCCATATTCTTCTTCATTTTGCATTGTC1130.10528277275691793No Hit
GACGTGGGATTCGCTTTCCATTTTTCAATGATTGCTCTCTTTCATTGCCTGA1120.10435106680331688No Hit
TTTACTGGGGTAAATGGTGCCTAAAAGATATTTGAAGAATAAGTGACCAGCA1120.10435106680331688No Hit
CGATTCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1120.10435106680331688No Hit
GCCTGGGGGGCTCAGCTGGCTAAGCCTTTAACATGGAAAGAAGGCTCATAAA1120.10435106680331688No Hit
ATATCCGGGGAGGAGGCGGGGCGCCTGTGGGAGCCGTGGAGGGAACTTTCCC1110.10341936084971584No Hit
TACTATTGGGGGGAGAGCGCGGGCGGCTGGGGAATGGCTGCTGCCATGGTGC1110.10341936084971584No Hit
AGCATGGGGGTTGTCGTTCCCCCTGCTTAAAATAAAGTTGTTTCTTAACTAT1110.10341936084971584No Hit
CTCAGCCGGGCATTTAAATAAACTGTCATCTTGTTGTTGGTGATGTTTTCTG1110.10341936084971584No Hit
TGATAAGGGATAGATATTCAAAATTTCCTACAAACTATAATTTTTTCCATGA1110.10341936084971584No Hit
TTATGGGGTGGCTGAGAACGGCTTCCTCCCAATTCACCTGTTGGGCCTCCGA1110.10341936084971584No Hit
AGTACCGGGACTGCATGTAAATGTTCTGAACTAAAGGTAGATAGCAATATGT1110.10341936084971584No Hit
ACGTTTGGGGACAGCTTGGTGCTGGTAAGAGGAAGCCCGTGGTTCTGGCTAG1110.10341936084971584No Hit
GTTTCAGGGATCTCGGGTATTTCTGAGCATATAAGAACTTTGAGAAGAGTTC1100.10248765489611479No Hit
CTAAATGGGACCCTCCCCAGGGGACTCCATCTGAGATGAGGCCTCGTCCTCC1100.10248765489611479No Hit
TTATAAGGGAGAGTGAGAATCTGGCTCCTGTTTCCATGGGTCAAAGCCGGTT1100.10248765489611479No Hit
ATGCTTGGGACGAGCATATTTCACCTCCGCTACCATAATCATCGCTATCCCC1100.10248765489611479No Hit
CTCGTAGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1100.10248765489611479No Hit
CTGAAGCGGGATGTGGGAAAGCCTTCAGCCGCAAAGCTACACTTGTCCAGCA1100.10248765489611479No Hit
ATTTGGGGGATTGGGACAGACCTAGTTCAATGAATCTGAGGAGGCTACTCAG1100.10248765489611479No Hit
CTGCTAGGGGTGTGTGGATGGATGAATGAGCAGTGGGAGTGCAGCGCCAGGT1100.10248765489611479No Hit
GCTAGTGGGGGTTTGAGAAATGGGAAGATGAATATTTACTCTGTAAATATGA1090.10155594894251374No Hit
TGTATAGGGTCACCTCTCAAATAAAAACATGCTGAAAAACTAGGACAGCAAA1090.10155594894251374No Hit
GTTAGTGGGCAGCGTTGTTGCGGGAAGTCAGGGACCCTGAACCGAGGAGCCA1090.10155594894251374No Hit
TCACAAGGGATCTAGCCAAACTAAGCTTCATAAGCAAAGGAGAAATAAAATC1080.1006242429889127No Hit
GGAAATGGGGATGACAGCACCATCCCGCTCACGGGAGCAGCAGGGGAGACAC1080.1006242429889127No Hit
AAGCCAGGGGTTGGGACCTGTTTTTTGGGGGACAGGGGCCCTGACTCAACTT1080.1006242429889127No Hit
ATTGCCGGGGCAGTTTCGTTTTTTCCTGACGCTTCTCTCCTTAGCTCACAGA1080.1006242429889127No Hit
AACTATGGGACATCTAAAACATTATTCTTTAATGGGATAATACAATTCATTG1080.1006242429889127No Hit
GATGCTGGGAGGCATTCACTTTGGCTTCTGAAATAATTCTAATAGCACAGAG1080.1006242429889127No Hit
ATACATGGGGCCATTCTGGAAGGTGTGAGATAATATATCATTGTGATTTTAA1080.1006242429889127No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCGTTCTACACTGTCTCTTA1080.1006242429889127No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)