FastQCFastQC Report
Sat 31 Dec 2022
SRR5844917.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844917.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106177
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTTCTACATCTCGTATGCCGTCT8310.7826553773416088Illumina PCR Primer Index 11 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACCGTTCTACATCTCGTATGC7040.6630437853772474No Hit
ATGCTAGGGGGGCGTCTAGAGGCTATTAAGGAAACAACTGTTCATTGGATGA2210.20814300648916434No Hit
AGTTAAGGGATTCCTTTGTAACTTGCTGGCATCATAAACTTGGATCAGGTTT1830.17235371125573334No Hit
TGACGCGGGATTGCTGGAAAGACCGTCCCAAAATTAGAGGTGCAATTAGTCA1700.16011000499166486No Hit
AGCGCAGGGATATGTAAACTAACGTGACATATAAAATGGATAAATACTTTAA1640.15445906363901785No Hit
AAAGTAGGGGTGGTTTAATATGGCTGTATGACTGGGGAAGTGGGAGTGGTAA1610.15163359296269438No Hit
ACCGGGGTGGCTGACCACGTGGCCGAGGAGCTCCAGCATCTGCTGCAGTGGC1610.15163359296269438No Hit
ACAGCAGGGATAATATAAAATTTTCATCTGAAAGTTTGTAGGAGTTTACATT1590.14974994584514537No Hit
TTGAAAGGGACGGTGGCAGAGGGAGGAAGTAACTCATACTAGGAAGAACAAA1570.14786629872759635No Hit
CTTGGTGGGGTATCCTGAGACTTTGGTGAAGTTGCTTATCAGCTTAAGGAGA1550.14598265161004736No Hit
TCAACCGGGACTGAGACTTGTGTATTTAATTTGGAAATGAGACTTCTGAGAC1530.14409900449249838No Hit
AAGTCAGGGGATCCCCGTCATCATGGAATGGTGCCTGGAGAGGAGGCTTCTG1530.14409900449249838No Hit
TGTGCGTGGGGGGGAGCAAAGAGGGCCTCAGAGAAGTCCTGACTCATAAAAA1510.14221535737494936No Hit
GTGAAAGGGCAAGAAAGGCTTGAAAGAAGTGAAAATAAACAGCCACGGGTGA1460.13750623958107688No Hit
ACTGGGGGGATACTGGGCAGTTCGAGACAGGTTTTTAATCATAAGTGGTCTT1460.13750623958107688No Hit
CTGCAGGGGACTCGCGCAGACCTAGCGCGTCCAGGTGGGAGGTTAGTGTGGC1450.1365644160223024No Hit
TTAGTCGGGGAAGATCACCAGTGGCATATCCAGGCATGCTGTGCTCTAACTG1440.1356225924635279No Hit
AGTTTGGGCTTCCGATCTAAGCCTGGGACTATTTTGAATTCATTCATTTCAT1430.1346807689047534No Hit
ATATCAGGGGAGATGTTAGATTTTTATCAAAAGTTTTTTCTGCATCTGGTGA1430.1346807689047534No Hit
CGTGGTGGGATTAAAATTCAAGAAATACATATTTAAGCATTTGTACAAAGAT1430.1346807689047534No Hit
GTACACGGGAAGAATGATGGTGGCTATTTCATTTTGGGGTATGGCTGTTGGT1420.13373894534597888No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTTCTACACTGTCTCTTATACAC1400.13185529822842987No Hit
CAAGATGGGTGTCAACAACTTTGTTGTTGGGAGTGAAGAAGGTTCTGTGTAC1400.13185529822842987No Hit
TTCTTGGGGAGGATATAGAAGACTTAGATCACTACGAGATGAAAGCAGAGCC1400.13185529822842987No Hit
TTGTGGGGGGGTTTTGCATTCTAAGACAAGAGTAATATTGTTTTAGACTGTG1400.13185529822842987No Hit
ATTTGTGGGACATCACACCTGTGACGGAAGCACTTCTGGAAGTGAACACTCG1370.1290298275521064No Hit
GGTAATGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1370.1290298275521064No Hit
CTGCTTGGGAAGGGTGAGTCTCACAGAGCTTTTAAGTTTGGCCTCTCACACT1360.1280880039933319No Hit
GTGCAGGGGAGGGTTCTGTATAAATAATAAATTACAATAACGATGGAGTGTC1340.12620435687578288No Hit
TATAATGGGAACTAACTTGTGAGTTGATTATGTATCCTGCAACTTTACCGAA1340.12620435687578288No Hit
CCTGACCTCGTGATCCGCCCGCCTCGGCCTCCCAAAGTGCTGGGATTACAGG1340.12620435687578288No Hit
ATGCTTGGGACGAGCATATTTCACCTCCGCTACCATAATCATCGCTATCCCC1320.1243207097582339No Hit
CAGCAAGGGCCACTTGGGAGGGCAGAAGCCAGGAATTCCACACCCTTGTGTT1320.1243207097582339No Hit
TTGTACGGGGGCTCTCGTCTGGACTTTTGAACTCCTGGCCACCATTCAACTC1310.1233788861994594No Hit
CTTAGCGGGAGAGGTTCAGAATTGGAAGTACAATTTCATTGCTTTTGCAATT1310.1233788861994594No Hit
GTACCTGGGAACCTTTTAAAGTGTAAAATCTTTGGATTTACAGTCACTGGTT1290.12149523908191039No Hit
GCCATAGGGTCATCTTGACTAAAATTATTTGAGCATTCATATTTTTTCCAAA1280.12055341552313589No Hit
GGACCGGATGGGCCATGGAGCCTTCCTGCAGCACGTGGTGCTGGCGGCCTGC1280.12055341552313589No Hit
TATCAGGGCTTCTATCTAGAATATATAAAGAATGCTTACAACTTCATACTAA1280.12055341552313589No Hit
GATACAGGGGTATTCATTCCCTTTAAAATGAAACATTCCTGGTTTATTTGTC1280.12055341552313589No Hit
AATTATGGGAATGGAACTGTGGTACACCCCAGACTTTGTATTAACAAGTTCC1260.1186697684055869No Hit
CACACCGGGTCTCTGTAGACTCAGTTATTGAAAATAGTCAAACACTTCAGAA1260.1186697684055869No Hit
TGGCCTGGCTGGCCCTGAGGGCTGAGAAGGAGAGGCTGCAGCAGCAAAAGCG1260.1186697684055869No Hit
TTGTCTGGGGATCTAAATTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCT1260.1186697684055869No Hit
GTTGGCGGGATATTAATGTTGAGAGAGAGGAATGGAAGTTGGATACACTTTG1250.1177279448468124No Hit
AAGCCTGGGCAGCGGAGTGAGACTCTGTCTCAAGGAAAGAAAGAAAAAGAAA1250.1177279448468124No Hit
GCAACCGGGATTCCTAATCCTGAAGTAGAGAGACTTAATACACACTTGTAGC1250.1177279448468124No Hit
TACAATGGGGGAGTTTTAAAAAAATCTTTGTGACCTTGAATAAACAAAGGTT1250.1177279448468124No Hit
GCCTAGGGGAAGTGAATATCGAGTTGTAGCACTGGATTTGAGAGGTTATGGA1250.1177279448468124No Hit
CAAGCTGGGGTCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAA1250.1177279448468124No Hit
GACAATGGGATATCAGAATGTTTCTATGTGCCACTTCCAAGGCATTTATCTG1240.11678612128803789No Hit
GTTTTAGGGATTGTCCTTTTAGTGCCTTAATTTGAGATAATTATTTTACTGC1240.11678612128803789No Hit
TGTATAGGGTCACCTCTCAAATAAAAACATGCTGAAAAACTAGGACAGCAAA1240.11678612128803789No Hit
TGAACTGGGGGACCTCTCCCTGATTACATAAGCTAAGGGGTCACATATGGCT1240.11678612128803789No Hit
CAATACGGGACATATCTTGTGCTGTGATATTAAAGTAGTATTCTGTTCTGGT1230.1158442977292634No Hit
ACCATGGGGGTAGTACTGGTTCTAACAAGTAACAAGCAAGTTTTAAAATTTT1230.1158442977292634No Hit
TACCAAGGGTTACGTTCCCTGAGAGGAATTTTGCCAATGAAGTTTAGGTACT1230.1158442977292634No Hit
GTATAGCAGGGAAACGACAAAAATTATTACCCAGGATGCTTAAAGATCTTCT1230.1158442977292634No Hit
CTCATTCGGGAACGTGGTGAAACCCTGTCTCTACTAAAAATACAAAAATTGT1220.11490247417048889No Hit
CTCAGCCGGGCATTTAAATAAACTGTCATCTTGTTGTTGGTGATGTTTTCTG1220.11490247417048889No Hit
CCATTAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1210.11396065061171441No Hit
TAGACGTGGGAATAACATCAAATGTAAATGGACTAAACTCTTCAATAAAAGA1210.11396065061171441No Hit
CAAGACGGGGGGCACCCGAGGCTCAGTAGAGGTTTAGTATGATGCCTTCCCT1210.11396065061171441No Hit
GTCGACGGGAGGACTTTTTCAAAGGTTTTTCAGAAACTTTGGAATTGGCAGA1210.11396065061171441No Hit
CTACAGGGGAAGCGCACAAACGGAAAGAGCGGGCGCGGTGCGCAGGGGCGGG1200.1130188270529399No Hit
CTTGTTGGGCAAATCAGATCTGGATCTATAATGAAGAGCATGAATTGGTTTT1200.1130188270529399No Hit
TAACCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1200.1130188270529399No Hit
TCATATGGGATCCACATCAGTTTGTGAGAAAAAAGTTAGTTCATGGCGTAAT1190.11207700349416541No Hit
AGAGCAGGGATACTACCCTACCATGCTTTTAATTCAGTAGTTGAATTCCTCA1190.11207700349416541No Hit
AGTGCTGGGATTACAGGCGTGAGCCACTGCACCCAGCCAAAAATTTTACATC1190.11207700349416541No Hit
GAGCCTAGACTCAGATTCATTTGGAGCAGCTAACTGCTCACCAAGAGCTTAT1190.11207700349416541No Hit
AAAATGGGGTAACCAGGTGCATATGTGGTTTTACACATGATGATGGATACAT1190.11207700349416541No Hit
TGCAGGGTCATCGCTATCCCCACCGGCGTCAAAGTATTTAGCTGACTCGCCA1190.11207700349416541No Hit
ATGTATGGGGACCGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAGGAG1190.11207700349416541No Hit
TGGTCAGGGGGGGCGCGAGGCGGCCACCGTGGAGAGCAGAGCGCGGCGGCTG1180.1111351799353909No Hit
CGATGGGGGAATCAAAGACATTTCATCCACCAATATCATGTGTAGATATTAT1180.1111351799353909No Hit
TTAGCTGGGTTATAGTAGCATAGTTACAACAACCATTCAATGCTTTGCAGTC1180.1111351799353909No Hit
CATTCAGGGTAAATAAATTGTAAATAATTTAGATTGGAGTGCTCTTTGTAAA1180.1111351799353909No Hit
TGTAAGGGGTGCAGTTATGGTTATAGCACAAGGCAATTGCCTGATGTAAAAG1170.1101933563766164No Hit
CTAATGGTGGGAAGGGAGAAAGGAGAGAGAAAGAAAAGAGAAAGAAAGAGAA1170.1101933563766164No Hit
GAGGTGGGAAGATTGCTTGGATTTGGGAGTTCAGGGCTGTGGTGGGCTGTGA1170.1101933563766164No Hit
CAAGTAGGGGAAGGGAAGGGCTAGAAGTTCTCATGAGGCTCCTCAAGAAGTT1170.1101933563766164No Hit
TGCGCTGGGTACTAAAGAAAATTTCCCAGTAGAACTTTAAAAGCTGTTTTTC1170.1101933563766164No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCGTTCTACACTGTCTCTTA1170.1101933563766164No Hit
TTTTGTGGGGGATTCCAGTGGCAATGAAACACATTTTACTGGGAACGAAGTT1170.1101933563766164No Hit
AGGCTAGGGGGAGGGATTTCGGCCTGAGAGCGGGCCGAGGAGATTGGCGACG1160.10925153281784189No Hit
CTAGTCGGGGGTTCTTTGTAGATGCATGTGTAAGAAGCTATTTTATAGCTTG1160.10925153281784189No Hit
TCTTTAGGGGCTGAATAGGCGATCACAACGTGCATTGTGCTTCTTTCTTTGG1160.10925153281784189No Hit
CTATCAGGGACGGGCTGCACTTTGGAATCACCTGAGGACCTTTCGGAATCTT1160.10925153281784189No Hit
TATGTAGGGGAAACAGTTAGCTTCAGTTAATTGCTAAGGATGTGGCAGAAGA1160.10925153281784189No Hit
CGCAAGGGTAAGTATATAAAATGAGTGTCGTGAAATCCTGTGCATTTACAAG1150.10830970925906741No Hit
ATTAGAGGGATGCTTCTAGTGGGCTGCTTCTGGCTACACCCCCAGATCCCTG1150.10830970925906741No Hit
AAAAATGGGAGCGACAGAAATCCGTACTTGCCTGCACTTTGAGGATAATTGA1150.10830970925906741No Hit
AAATGTGGGAACCCAATGAGAACCAGATCTCTGGTGGAGAGCCATCCTCTAC1150.10830970925906741No Hit
GTAGAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1150.10830970925906741No Hit
TAGTTTGGGCTAACTTCCCAGGTGACAGTAGCAGGAACAAGGAAGGGTAATG1150.10830970925906741No Hit
CGGTTTGGGAATAAGGCTACATCTGAAGACTAAAATAGCCTAAGCTCTTGAG1150.10830970925906741No Hit
ACAATGGGGACCCATTGACCCACCTAATTATAAAATTTAGGAATATAATTGT1150.10830970925906741No Hit
AGACGGGGGAAAGAATCTGTACTGCAAGTAAAACCTACTCCCCAAAAATGTG1140.10736788570029292No Hit
TTGTTGGGGCATGTGTCACCCATCTTTTCATATCCATTTCTATGGTATTTGG1140.10736788570029292No Hit
TGAATAGGGACCTCTATTTTCTGTGGTTTTTGCTTTGTTTTCATGGGAAGCT1140.10736788570029292No Hit
GAGCATGGGCAGATTTCAGTGATACGAGAAAGGGGACTGGTCATCTATAGAA1140.10736788570029292No Hit
TTGTCCGGCTGCTGAAAGAATAGAGTCTTAAACTTTAATAATCACAGCCTTG1140.10736788570029292No Hit
AGTAATGGGATAATCGGTAAATTAAGAATTGCTACTCGAAGGTGCCAGAATG1140.10736788570029292No Hit
GCTGCCATGGGTGAGTTTGTGTTCGTGAATGCCCTGGTGACAAGAGTCTCTT1130.10642606214151841No Hit
TTATAAGGGAGAGTGAGAATCTGGCTCCTGTTTCCATGGGTCAAAGCCGGTT1130.10642606214151841No Hit
CTCATTGGGGCTTTTTCCCATCTTGCAAGATGGCGGGTGAAAAAGTTGAGAA1130.10642606214151841No Hit
CAGTCCGGGAGGCGTAGGCCATGACCATTTGGGGTACGAGAGCCTAATTTTG1130.10642606214151841No Hit
TCACAAGGGATCTAGCCAAACTAAGCTTCATAAGCAAAGGAGAAATAAAATC1120.1054842385827439No Hit
AGGCCTGGGGAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCG1120.1054842385827439No Hit
CTCAGTGGGTGGCTACCTAATTGGTGTCTTTATGTTTTAGATGTTTTTGTGG1120.1054842385827439No Hit
AACTAAGGGTTACTGAAAACCAACTGACTACTGATGTCTCATGTTGGTGTAT1120.1054842385827439No Hit
CTGCCCGGGCCGCGGAGGAGGCGCCGCTGGTGTGTCCCTTGGTGGAGAGGGC1120.1054842385827439No Hit
TTTTAGAGGGAGGGGCAGAACAGATAGATTACGACAGGTTTGGTTTTTAAAT1120.1054842385827439No Hit
GCGCTAGGGGAGGCAAACACCGAGCTCCTCCAGAGCCGCCCGGGAGCAGGGA1110.10454241502396941No Hit
TCTAGCGGGGGAGGCCCCCAGGGAGGGGCCCAGCGCCTCCCGCGGGCCGCCT1110.10454241502396941No Hit
AGTTGTGGGATACTTTATCACTTAACATGATATTGGCATTTTTGCCACTAAA1110.10454241502396941No Hit
TTGCAGGGGGATTCTCAGCCCTCGGTCAAAAGAATCTGTTCCAGAATTCCCC1110.10454241502396941No Hit
GAAACTGGGATCCTATTGATTTGTAATCAATCAGGTTGTTTTCAGGATTTTT1110.10454241502396941No Hit
AACGCAGGGAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGT1110.10454241502396941No Hit
AAGTTTGGGCTAAATGGCCACTTCATACACAGAGTGGCTGAAATCAACATCA1100.1036005914651949No Hit
GATACTGGGGAGATTCAGAGATACAAAAAGAATCACAGAAAAGAAAGGCAAT1100.1036005914651949No Hit
ACTCCAGGGATCCTCATGTAAGAGAGGATTCTCCAGTCATGTGATTTGCTCA1100.1036005914651949No Hit
CTCCAAGGGTGGTAGATCACTTGAGGTCAAGAGTTGTGACACCAGCCTGGCC1100.1036005914651949No Hit
TTTACTGGGGTAAATGGTGCCTAAAAGATATTTGAAGAATAAGTGACCAGCA1100.1036005914651949No Hit
GATGCTGGGAGGCATTCACTTTGGCTTCTGAAATAATTCTAATAGCACAGAG1100.1036005914651949No Hit
GGTTTTGGGCTCTTTGATCCTATTTTCTATGTCCTTGAATGCTTTTGCTCCT1100.1036005914651949No Hit
ATACTCGGGGAGGCTTTCAACAGCGGGATGGATCAGGCGGAGGAAGGAATCA1090.10265876790642042No Hit
TGTGCTGGGTTTGCAGGTGTGAACCATCACGCCTGGCCATTGATAAGCATTT1090.10265876790642042No Hit
ACGTTTGGGGACAGCTTGGTGCTGGTAAGAGGAAGCCCGTGGTTCTGGCTAG1090.10265876790642042No Hit
CATCCAGGGATGCCAGTATCTGCCAGCAGAATGGCATTGTGCCCATCGTGGA1090.10265876790642042No Hit
AAATATGGGGGTATCATCAAGCTCAACGTTCTGGAGTGGCTGCAGCTCACCA1080.10171694434764592No Hit
GCGTGAAGGGCCCTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGG1080.10171694434764592No Hit
ACACACGGGAAAGTCTAAATGAAGAAGCTGCCTGAGATTGTGGCTTTCAGGC1080.10171694434764592No Hit
TAAGAGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1080.10171694434764592No Hit
GAGCCTGGAGATTTACGGTTCCGAATCAAAGTTGTCAAGCACCCAATATTTG1080.10171694434764592No Hit
TCTGCACGGGTACACCTTCCTCATGAAAATATTTTTTAAAAGAATGGAAGAA1080.10171694434764592No Hit
TATTAAGGGTATCGGTCTTGGGAGATCAACCCTGAACCTGGAATGTGCTGGG1080.10171694434764592No Hit
TCTCGGAGGGCATGTGACCAGTTAGAGTGTAAATCTTCATTTACTACATATT1080.10171694434764592No Hit
ATATGTGGGATGCGGCAAGAGGCAACTGGCTCCACAGCCTGGGATGCTGCCG1070.10077512078887141No Hit
ACGCAGGGGGACAAAAAAGAATAAAAAGCAGTGAAGATGACCTACAGGATGT1070.10077512078887141No Hit
GACGTGGGATTCGCTTTCCATTTTTCAATGATTGCTCTCTTTCATTGCCTGA1070.10077512078887141No Hit
GCTAAGGGAGATCGTGGTCCTTCTGCAGCGCTTGAAGCCTGAGATCAAGGAT1070.10077512078887141No Hit
TGATAAGGGATAGATATTCAAAATTTCCTACAAACTATAATTTTTTCCATGA1070.10077512078887141No Hit
CTCGACGGGTAACTGCATGATTTCTGTTTTATCTACCTCTAAAGCAAATCTG1070.10077512078887141No Hit
CGATTCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1070.10077512078887141No Hit
ACGGAGGGGCTTTTGGCTCTCTGACCAGCACCATGGCGGTTGGCAAGAACAA1070.10077512078887141No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)