FastQCFastQC Report
Sat 31 Dec 2022
SRR5844865.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844865.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67630
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGGTGGCTATCTCGTATGCCGTCT5320.7866331509685052RNA PCR Primer, Index 20 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGGTGGCTATCTCGTATGC4630.684607422741387No Hit
TGTTTTGGGACCTCATTCTTTGTGTCTTCAGTGTTGGCTTGTTTTAGCTGAT1220.18039331657548424No Hit
GCTCTTGGGAGTCAGTGGGCCGAAGAGGAAATCAAAAGAGAAATAAGAAAAT1070.15821381043915422No Hit
GTATCAGGGGACCCTGTCTAGAAAAAAAACAAAGAAAAGAAAACTTCCCCAA1030.15229927546946623No Hit
CAGTTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC950.1404702055300902No Hit
TTCGAAGGGCTTTATGCAAAACCAGATGGGCATATGGTTCACAGACGGAGAT950.1404702055300902No Hit
GTTAGGATTAGTCATAAGACTGTCAGTAATCCTGAAGATGGATGAGATAATC920.1360343043028242No Hit
TATATTGGGACCAAAAACCAGTGAAAGCAGTACTAAGAGGAAAGTTTATAGC910.13455567056040219No Hit
GTTTGGGACATGTAGGGGGACAACTAAGAGATCATTTGCTTCTTTCGGCGTA900.1330770368179802No Hit
TTGCATGGGCAAGGATCAAAGTAAAGGTGAGTTTATAGAATGCTCACTCTAG860.12716250184829217No Hit
TTTGTAGGGACATGAAAATATAAAACACATTGGTAAAGCAATCACACAAAAA850.12568386810587018No Hit
TGTAGAGGGGCTGCCCCGCAGCGCGCAGGGACCATGTCGGCGGAGACCGCGA840.12420523436344817No Hit
ACCCTAGGGATTGTTGTGATGGCCATCTGCTGGAAACTCCTGAAGAGCATTA840.12420523436344817No Hit
CTTCCGGGATGCTTATTAAAGTTTGAGAAGCAAATTTTGTTTTGGTGCCATG820.12124796687860417No Hit
CTGTGAGGGATGTTAAATTAACCAAACACTGGATTGTTGAAAAATATTTAGT800.11829069939376016No Hit
CTTTTTGGGAGACTACAAAACAACAGGATGAATGAAGCAAAAGTTGATTTTT790.11681206565133816No Hit
GGTAGCGGGAAAGGATATCAGAGATTGAAGACTACCTTACTGAAATAAGATA780.11533343190891615No Hit
GTGCTGGGGAGGCTGTGGTAGGAGGGTTGTTTGAGCTTGGAATATTGAGGCT780.11533343190891615No Hit
GGTTGGGGTATTCAAATGGAAGACTCCACTCTGTGCCTAGGTCATAGGCTGT780.11533343190891615No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGTGGCTATCCTGTCTCTTATAC780.11533343190891615No Hit
CTTTGAGGGCTCATCCATGTGTACATTATTCATGCATTTACTTTTCTGCCAC770.11385479816649416No Hit
TTAGGGGGGAAGTTATATTTACATATGCTTTTTTAAAGTTATGCCTTGTGAA770.11385479816649416No Hit
TGGCTGGGAGTCACTATGCAGTCTATGCATGTAGCAAAATTGCACACATACT760.11237616442407215No Hit
CTATTAGTGGGATATGATATGTATACCAAACTCCAGCCAACTTACTGCCATT760.11237616442407215No Hit
TGCGCAGGGACAGACAAAACGATAAAGTTGCAGATCTCTGGAAATGAATTCA750.11089753068165016No Hit
GGCATGGGGATGCAAATTTCTGCAGGAGGCTTGAGTTTCTCCCCAGAAAATG750.11089753068165016No Hit
TCGACAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA750.11089753068165016No Hit
AAACAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA750.11089753068165016No Hit
GATGGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC740.10941889693922816No Hit
AGCCGTGGGTCTGAGACTGGAGAGAAGAGAGTAAATATTTCATTCTCAAAAA740.10941889693922816No Hit
TCATGAGGGATATATGGTCTAGTTAGTATTGCTGGTATAAAAGTTAGTATTG730.10794026319680616No Hit
GCAGCTGGGATTACTCAAATGGTTGCTCTAGAATTTAAGACCAGTTTTATCT730.10794026319680616No Hit
CTACCTGGGGACTTTCCGTCATGGCGCTGAAGGTAGCGACCGTCGCCGGCAG730.10794026319680616No Hit
TTGCCTGGGGATAACAAAAAATTCTTAGCAAACTAGGAATATAAGAGAGTTA720.10646162945438416No Hit
GTTTATGGGAGAAAGATTTCCAGGATATTATCACCCATAAAGATATATTAAA710.10498299571196215No Hit
CTGAAAGGGCAGTTGATGGACATTTGGGTTGTTTCCACTGCTTTTTTAAAGA710.10498299571196215No Hit
ATTGTCAGGGCACCACTAGGAGAAATGCAGAGTTAAAGGTCACCATCAAAAA710.10498299571196215No Hit
CTTTAAGGGAGCAAAAAAGTGGGGGGCATATTGTAGTCCTGTCATTTAAGTT700.10350436196954013No Hit
GAAGTAGGGCTAGCTATGTAATTGGCTAAAATGCAGGAGTTCTGCTGTTAAA690.10202572822711814No Hit
CAAACGGGGAGAGAGATAGAGTTTAGCACTACAGTGACTTAGATGTATGTTG690.10202572822711814No Hit
ACACATGGGGTAAAACTGGTTTTTTAAAATCTGTATGTATGTATTACTTTGA690.10202572822711814No Hit
TAAGCTGGGAGGAGAGAAAAGAGTCTAAACTTTCATTCCATGGATGACAAAA690.10202572822711814No Hit
GCACGAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA690.10202572822711814No Hit
AATAAGGGATTTGAGAAAGGGAGTGAGAAGAAGGAGGAGGAAGAAGGAGGGT680.10054709448469613No Hit
CTAGTCAGGGGGAGAATTCAGTGACATGTCAAATCACTATTGCAAATTAAGT680.10054709448469613No Hit
TTGGCAGGGAAGAAATCCAAGCTGGAGTTCTCCATTTACCCGGCACCCCAGG680.10054709448469613No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)