FastQCFastQC Report
Sat 31 Dec 2022
SRR5844864.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844864.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68257
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGGTGGCTATCTCGTATGCCGTCT4770.6988294240883719RNA PCR Primer, Index 20 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGGTGGCTATCTCGTATGC4110.6021360446547607No Hit
TGTTTTGGGACCTCATTCTTTGTGTCTTCAGTGTTGGCTTGTTTTAGCTGAT1420.20803727090261803No Hit
GCTCTTGGGAGTCAGTGGGCCGAAGAGGAAATCAAAAGAGAAATAAGAAAAT1330.19485181007076197No Hit
GTATCAGGGGACCCTGTCTAGAAAAAAAACAAAGAAAAGAAAACTTCCCCAA1160.1699459396105894No Hit
TTTGTAGGGACATGAAAATATAAAACACATTGGTAAAGCAATCACACAAAAA1070.1567604787787333No Hit
TTGCATGGGCAAGGATCAAAGTAAAGGTGAGTTTATAGAATGCTCACTCTAG880.1289245059114816No Hit
TTGCCTGGGGATAACAAAAAATTCTTAGCAAACTAGGAATATAAGAGAGTTA850.12452935230086293No Hit
TTAGGGGGGAAGTTATATTTACATATGCTTTTTTAAAGTTATGCCTTGTGAA850.12452935230086293No Hit
GTATTTGGGATTCTGTATCTGAAAACTATTAAATGATCTGATTCAGCAACAA840.12306430109732336No Hit
GGCATGGGGATGCAAATTTCTGCAGGAGGCTTGAGTTTCTCCCCAGAAAATG840.12306430109732336No Hit
TGTAGAGGGGCTGCCCCGCAGCGCGCAGGGACCATGTCGGCGGAGACCGCGA840.12306430109732336No Hit
GCACGAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA840.12306430109732336No Hit
TTCGAAGGGCTTTATGCAAAACCAGATGGGCATATGGTTCACAGACGGAGAT830.12159924989378378No Hit
CTTTGAGGGCTCATCCATGTGTACATTATTCATGCATTTACTTTTCTGCCAC810.11866914748670467No Hit
ACATTTGGGGTCTGAGGTGGTAGCAGCCAAAGCGGTGAGAAGCATTCAGATT800.11720409628316511No Hit
CTTAACGGGGCAGCAACAACGTGTTGTACACTTAAAAATTTGTTCAGAGGAT790.11573904507962553No Hit
TCAGTAGGGATGACATCCTTGATCTTCATTTTTTGGACTCTCAATGAAACAA790.11573904507962553No Hit
CTTCCGGGATGCTTATTAAAGTTTGAGAAGCAAATTTTGTTTTGGTGCCATG770.1128089426725464No Hit
TATATTGGGACCAAAAACCAGTGAAAGCAGTACTAAGAGGAAAGTTTATAGC770.1128089426725464No Hit
CTGTGAGGGCAAAAGCAAAACGAGATTTAATTAAACATAAGAGCTTCTGCAT760.11134389146900685No Hit
GGTAGCGGGAAAGGATATCAGAGATTGAAGACTACCTTACTGAAATAAGATA760.11134389146900685No Hit
GCAAGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC760.11134389146900685No Hit
CACTAGGGGTGGATGGCAGAGATGGTTTTTTAATGAAATCCCACCATCTATC750.10987884026546726No Hit
GAGCATGGGACTGTGAGAAAAAAAATTGGGAAAGCAGTACATCATTTCTGAA750.10987884026546726No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGTGGCTATCCTGTCTCTTATAC750.10987884026546726No Hit
TGCGCAGGGACAGACAAAACGATAAAGTTGCAGATCTCTGGAAATGAATTCA740.10841378906192771No Hit
ACTTGTGGGAGCTCTAAAATTCAGTGCCTTGGTAATAAAATATCACTTCTTT740.10841378906192771No Hit
TTGGCAGGGAAGAAATCCAAGCTGGAGTTCTCCATTTACCCGGCACCCCAGG740.10841378906192771No Hit
GTAACAGGATTGCTTCTTTATCGAACTTGAGTGTGGCAATAAAACTGGAAAC730.10694873785838815No Hit
TTTATAGGGGGAAGGGAAGAGATCTTAATAAGAGAGTGTCTGAACTCAGTTT730.10694873785838815No Hit
ACAGCGGGATCTGGTGAGGGCCCGTTCCTCATTGACAGTCATCTTGCTGTAT730.10694873785838815No Hit
TATAGCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA730.10694873785838815No Hit
GCTTCGGGGGTGATGATCCCGACACTATTAGAAGCAATCTTCCCCATATTAA730.10694873785838815No Hit
AATTTTGGGAATAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACTTAA730.10694873785838815No Hit
CTACCTGGGGACTTTCCGTCATGGCGCTGAAGGTAGCGACCGTCGCCGGCAG730.10694873785838815No Hit
CTCACCGGGATTCAGTCTTAAGAAAAGTTGAGAAATGTCCATCTTTAAGTGT720.1054836866548486No Hit
TGTGACGGGTCCTCATGGCTGTTATCTGTCTTTATGATTTCATGATTAGACA720.1054836866548486No Hit
CTCATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA720.1054836866548486No Hit
CGGTCAGGGAAGATTTGAATGGCTTTTGTTGTTAGGGGGCTTTTTTATGAAG720.1054836866548486No Hit
CTGTGAGGGATGTTAAATTAACCAAACACTGGATTGTTGAAAAATATTTAGT710.10401863545130902No Hit
CAGTTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC710.10401863545130902No Hit
TCGTATGGGTACAACTCTTCCTTTCACTTGAACATTTAGAGGCCATAGTAGG700.10255358424776945No Hit
ACCCTAGGGATTGTTGTGATGGCCATCTGCTGGAAACTCCTGAAGAGCATTA700.10255358424776945No Hit
GGTCATGGGAGACACCAATTCCTCTGCTGCACAGACTTTTAAACCTGGAAAG690.10108853304422989No Hit
GCGGATGGGGATTATCCCCAAAGATATTACAGGGTAGACACGTTTTAACTGA690.10108853304422989No Hit
TTAACCGGGGATGCCAAGTCACATCATATTTTAAGTGAATGCAGAGCTTGGT690.10108853304422989No Hit
TGTTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA690.10108853304422989No Hit
ACACATGGGGAAATCAATCAGGAGGAATTTCAGAAAATTCATAAATATCCAG690.10108853304422989No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)