FastQCFastQC Report
Sat 31 Dec 2022
SRR5844863.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844863.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67146
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGGTGGCTATCTCGTATGCCGTCT4680.6969886515950316RNA PCR Primer, Index 20 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGGTGGCTATCTCGTATGC4250.6329490959997617No Hit
GTATCAGGGGACCCTGTCTAGAAAAAAAACAAAGAAAAGAAAACTTCCCCAA1350.2010544187293361No Hit
GCTCTTGGGAGTCAGTGGGCCGAAGAGGAAATCAAAAGAGAAATAAGAAAAT1280.19062937479522235No Hit
TGTTTTGGGACCTCATTCTTTGTGTCTTCAGTGTTGGCTTGTTTTAGCTGAT1210.18020433086110862No Hit
TTGCATGGGCAAGGATCAAAGTAAAGGTGAGTTTATAGAATGCTCACTCTAG1120.16680070294581956No Hit
TTTGTAGGGACATGAAAATATAAAACACATTGGTAAAGCAATCACACAAAAA1030.1533970750305305No Hit
TATATTGGGACCAAAAACCAGTGAAAGCAGTACTAAGAGGAAAGTTTATAGC1020.1519077830399428No Hit
GCTTCGGGGGTGATGATCCCGACACTATTAGAAGCAATCTTCCCCATATTAA940.13999344711524142No Hit
CTTTGAGGGCTCATCCATGTGTACATTATTCATGCATTTACTTTTCTGCCAC890.13254698716230304No Hit
CAGTTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC880.13105769517171537No Hit
TTCGAAGGGCTTTATGCAAAACCAGATGGGCATATGGTTCACAGACGGAGAT880.13105769517171537No Hit
CAAACGGGGAGAGAGATAGAGTTTAGCACTACAGTGACTTAGATGTATGTTG840.12510052720936468No Hit
GCAAGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC840.12510052720936468No Hit
ACACATGGGGAAATCAATCAGGAGGAATTTCAGAAAATTCATAAATATCCAG790.1176540672564263No Hit
AGCCGTGGGTCTGAGACTGGAGAGAAGAGAGTAAATATTTCATTCTCAAAAA780.11616477526583861No Hit
GGTCATGGGAGACACCAATTCCTCTGCTGCACAGACTTTTAAACCTGGAAAG770.11467548327525094No Hit
GCACGAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA770.11467548327525094No Hit
CTGTGAGGGCAAAAGCAAAACGAGATTTAATTAAACATAAGAGCTTCTGCAT760.11318619128466327No Hit
CTACCTGGGGACTTTCCGTCATGGCGCTGAAGGTAGCGACCGTCGCCGGCAG760.11318619128466327No Hit
AATTCCGGGACAATTTCACATGGCGCAACGATGTGGCTATTTTTGTCTCAAA740.11020760730348791No Hit
CAGCTGGGGGCTGCCATGCGGCAGACCCCCCAAAATCCCTCAGTGTCTTCCT730.10871831531290026No Hit
TACCTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA730.10871831531290026No Hit
TGTAGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC730.10871831531290026No Hit
ACACATGGGGTAAAACTGGTTTTTTAAAATCTGTATGTATGTATTACTTTGA730.10871831531290026No Hit
GGTAGCGGGAAAGGATATCAGAGATTGAAGACTACCTTACTGAAATAAGATA730.10871831531290026No Hit
GTCCGTGGGCAGATGGTGAAATGTGACCCTCGCCATGGTAAATACATGGCTT730.10871831531290026No Hit
TAACTTGGGGGCACCAGTTGTTTCTGTTTCTCATCTCCCCCCTACCTTCAGT730.10871831531290026No Hit
CTCAGTGGGCTGGACATGAATTTCTAAAATGCCTTAATAGGTTTATGTAGTT720.10722902332231257No Hit
GGTTGGGGTATTCAAATGGAAGACTCCACTCTGTGCCTAGGTCATAGGCTGT710.1057397313317249No Hit
AATTTTGGGAATAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACTTAA710.1057397313317249No Hit
TTAGGGGGGAAGTTATATTTACATATGCTTTTTTAAAGTTATGCCTTGTGAA700.10425043934113722No Hit
TCATGAGGGATATATGGTCTAGTTAGTATTGCTGGTATAAAAGTTAGTATTG690.10276114735054956No Hit
TAATGGGGACCTGCAGACATGTGTCATATGAATAAATCAGATAAGAGCAAGC690.10276114735054956No Hit
TGTAGAGGGGCTGCCCCGCAGCGCGCAGGGACCATGTCGGCGGAGACCGCGA680.10127185535996187No Hit
CTTAACGGGGCAGCAACAACGTGTTGTACACTTAAAAATTTGTTCAGAGGAT680.10127185535996187No Hit
TGGAAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA680.10127185535996187No Hit
TATAGCGGGTTGATCTTCTCTATCTTAAAATTGCTTTGTATTTTGTTGATTT680.10127185535996187No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)