FastQCFastQC Report
Sat 31 Dec 2022
SRR5844853.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844853.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189014
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGGTGGCTATCTCGTATGCCGTCT6280.3322505211254193RNA PCR Primer, Index 20 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGGTGGCTATCTCGTATGC6150.32537272371358733No Hit
CGCAACGGGTATACTGGCAACGAAGAAGTGGAATTTGAAGTTAAAAACACAT2410.12750378278857652No Hit
TTAATGCGGGACTAGTTTTTCAGCAAACTTACTTTTTCAACTATAGAAAAAC2070.10951569724993918No Hit
AAGAGAAGAGTTCAATATATTTTCACAGATACGGTTTTAAAACTTCAGAGAA2030.1073994518924524No Hit
GATGCTGGGTCATTTTCTGATGTTGGAAATAAGATCAAGGTGGGAAACAATA2020.10687039055308073No Hit
CTCACTGCTATAATAAATTACCACAAACCAAGAGGCTTAAAACAGCACACAT2020.10687039055308073No Hit
TGTAGTGGGCCTCCTCTCTCCCCAAAATTATTGGATCAAAAAAGAAATTCCA1980.10475414519559398No Hit
TGAAAGCGGGAAACACTGTAAACTTAGGCTACTTTAAATTTATGAGAAGATA1980.10475414519559398No Hit
CACTGAGGGGTGTCAGACAGAATTCAACCAGCCCTGCATATTCAGAGAAGAC1950.10316696117747891No Hit
AGCTATGGGACCTGCTAGCTGGCATGGATCCTTAAGGAAGCAGGAGGGAGTT1950.10316696117747891No Hit
AGGTTGGGGTACCGAAAGTATCTGGAGCTAAGCAAAAATGGCACTGAGCCTG1950.10316696117747891No Hit
GTACCAGGGCTCTAGTGTGTTGATAAGCATTGGAAGAGTCTGCCTACTCAGC1940.10263789983810724No Hit
AAAATTGGGGGAGGGTGTCAAGAGGTATGAACAGGAGCATGCTGCTATCCAG1920.10157977715936385No Hit
CATCCCGGGGAATCTACAAGAACCAAACTCCTAAGATGAATAAGTGAGTTCA1910.10105071581999216No Hit
GAAACCGGGCAGAGCTCCCAAGTGATGGACCCTCCCACAGCCAGGCCTACAG1910.10105071581999216No Hit
TAGTATGGGGGACCACTCTTAGAAGGGGGTCAGAGCTGGTGAAGAAATGCTG1910.10105071581999216No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)