FastQCFastQC Report
Sat 31 Dec 2022
SRR5844852.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844852.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189508
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGGTGGCTATCTCGTATGC6490.3424657534246575No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGTGGCTATCTCGTATGCCGTCT6430.3392996601726576RNA PCR Primer, Index 20 (95% over 22bp)
CGCAACGGGTATACTGGCAACGAAGAAGTGGAATTTGAAGTTAAAAACACAT2590.13666969204466303No Hit
AAGACAGGGCCGAAGCATCTTTTGGTGTCTGCCAGAGATTAAAGAAATCCTC2160.11397935707199697No Hit
GTGCCGGGGAGGAAAAGCTCTAAGACACTAATATATGTATCTTATAAACAAA2080.10975789940266374No Hit
CATCCCGGGGAATCTACAAGAACCAAACTCCTAAGATGAATAAGTGAGTTCA2080.10975789940266374No Hit
CTCACTGCTATAATAAATTACCACAAACCAAGAGGCTTAAAACAGCACACAT2070.10923021719399709No Hit
TCATGGGGGACCTTCCTTCTAGCAGAAATGGCGGCTGCGGCGGCTCGAGTGG2050.10817485277666379No Hit
TTTTGGCGGGGGGTGGGGAGACAGCATGCCACTGGAAAAATTCGTAGACATG2010.10606412394199717No Hit
AGCCATGGGAGCCGTGGATTTTGGTATCCATGAGATGTCCTGAAACCAATTC2000.10553644173333052No Hit
CACTGAGGGGTGTCAGACAGAATTCAACCAGCCCTGCATATTCAGAGAAGAC1990.10500875952466387No Hit
AAGAGAAGAGTTCAATATATTTTCACAGATACGGTTTTAAAACTTCAGAGAA1990.10500875952466387No Hit
GTGTCCGGGAGATAAGAGAGCAGATGTTGGAGAATTCTTCTAGATTTTCAGA1980.10448107731599722No Hit
AGCTATGGGACCTGCTAGCTGGCATGGATCCTTAAGGAAGCAGGAGGGAGTT1980.10448107731599722No Hit
ACGCTTGGGGCTCATTAGCACACTTCGGATGTAGTAATTAATCATTTGGATC1950.10289803068999726No Hit
TTCGCGGGGAGAACCATTTGGAAAAATGAAATTTAGTAGTTCCAAGTTTCAA1950.10289803068999726No Hit
TGTAGTGGGCCTCCTCTCTCCCCAAAATTATTGGATCAAAAAAGAAATTCCA1940.1023703484813306No Hit
AGAGTAGGGATCCAGAAAGAGTCCACCCTGCACCTGGTGCTCCGTCTTAGAG1940.1023703484813306No Hit
TGAAAGCGGGAAACACTGTAAACTTAGGCTACTTTAAATTTATGAGAAGATA1910.10078730185533065No Hit
CAGTTTGGGAGGGTTCAAATATGTATCTGTTTGACTCTAAAGTCTGTGCTTA1900.100259619646664No Hit
AAGTCAGGGAGCATTTTTACTTTATCTTCATGTTTTCACAAATGATATCACC1900.100259619646664No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)