FastQCFastQC Report
Sat 31 Dec 2022
SRR5844851.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844851.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189582
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGGTGGCTATCTCGTATGCCGTCT6390.33705731556793367RNA PCR Primer, Index 20 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGGTGGCTATCTCGTATGC5740.30277136015022527No Hit
CTCACTGCTATAATAAATTACCACAAACCAAGAGGCTTAAAACAGCACACAT2430.12817672563851No Hit
AGCTATGGGACCTGCTAGCTGGCATGGATCCTTAAGGAAGCAGGAGGGAGTT2210.11657224842020868No Hit
TGTAGTGGGCCTCCTCTCTCCCCAAAATTATTGGATCAAAAAAGAAATTCCA2200.11604477218301315No Hit
AAGACAGGGCCGAAGCATCTTTTGGTGTCTGCCAGAGATTAAAGAAATCCTC2170.11446234347142661No Hit
AATTAAGGGACCAGATTGCGTGGTCAGCTGACAGTCGGCTCCTGGTCAGCGG2160.1139348672342311No Hit
CGCAACGGGTATACTGGCAACGAAGAAGTGGAATTTGAAGTTAAAAACACAT2110.11129748604825354No Hit
CTGCGGGGGTAGATTTGTCAGTATTGAACTATCCTTGCCTTCTGGTATGAAT2080.10971505733666698No Hit
TGTCAGGGGATTTACTCTATTTATGCAGCCAAAAGACACATGAAAAAATGCT2070.10918758109947146No Hit
AAAATTGGGGGAGGGTGTCAAGAGGTATGAACAGGAGCATGCTGCTATCCAG2060.10866010486227594No Hit
ATAACAGGGCCACGAGCAGCCGAGGGGCGGGGAGGACGCTGCTGTCCAGGAG2060.10866010486227594No Hit
TCGCTAGGGAAATAAGAGAAATTTCAAAGTGATCTCAATACCTTCAGTCCCA2060.10866010486227594No Hit
TTCCACGGGAAACAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAACT2020.1065501999134939No Hit
AGCCATGGGAGCCGTGGATTTTGGTATCCATGAGATGTCCTGAAACCAATTC2010.10602272367629838No Hit
ACGTTGGGATGTTCAATATTTCTCCATTAGGTATGATCTTAGCTATAGGGTT1990.10496777120190737No Hit
CCAATTGGGATATCCAACATTTTGTTTTCTGGGGTAATTTATTTTAAAATAC1980.10444029496471184No Hit
ACAAGAGGGAATGATCTATTGCCTACAGGAAACACACTTCATTTATAAAAAC1960.1033853424903208No Hit
GTGCCGGGGAGGAAAAGCTCTAAGACACTAATATATGTATCTTATAAACAAA1960.1033853424903208No Hit
GTATGCGGGACTCAAAACCCTGAGCTATTTACTAAAGCAGGAAGATAGCCTG1960.1033853424903208No Hit
CGCTATGGGATTCTAACTGATACGTAGACACTTACTTGGAAATTTTTGGACA1900.10022048506714773No Hit
GAAACTGGGACCACAGGCTGGGTGGCTTCAACAGCAGAAATGAATTTTCTCA1900.10022048506714773No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)