FastQCFastQC Report
Sat 31 Dec 2022
SRR5844846.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844846.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142991
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACACTTTGCGATCTCGTATGC5270.36855466427957007No Hit
CTTATACACATCTCCGAGCCCACGAGACACTTTGCGATCTCGTATGCCGTCT5090.3559664594275164No Hit
GAAATGGGGACTCTGAATTCAGTGCTGCTTTGGGACATATACTTGACCTAGC1780.12448335909253029No Hit
TATCGAGGGAGCTGTTTTTCGTCTTCCCTAGGCTATTTCTGCCGGGCGCTCC1680.1174899119525005No Hit
TCGCTCGGGCAATAAGACTTTATTTACAAAAACAGCAGGCACAGACAAGATT1620.11329384366848264No Hit
TGCCGAGGGTGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1590.1111958095264737No Hit
TCTACTGGGGAATCCAGAGATTAAAAACAGAAATATGGAAAAGCTATGTACT1570.10979712009846773No Hit
TCTATGGGAACTTCACAAGGGAGGTACTGGCATTATCTCACTTCATAGATGA1550.10839843067046177No Hit
GCCTAAGGGCGATCTTTCTAGGGGAGGCGGAGCATGTGGTGAGTTGAGCCGC1550.10839843067046177No Hit
AACACCGGGGGCGGCCACCGTGGAGAGCAGAGCGCGGCGGCTGGAAGCTGCT1540.1076990859564588No Hit
CTTTAGGGGCCCAGGCATGAGCCACTGCACCTGGCCAGCTCTTAATTAACTA1530.10699974124245581No Hit
AGGGCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAAG1520.10630039652845284No Hit
GTGTGGGGGGGGAGATGTGGTGAAAATTGCAAGAGAACTAGAATTGGAAGTG1510.10560105181444987No Hit
GACCTTGGGAAAGAGATTGATTAGTCACATATCAGCATTGCCCCAGGTGCTG1500.10490170710044687No Hit
TACAGACGGGCTTGAAAAGGCGTTGGTATCTTCATTTTACAGTTCATTAACC1500.10490170710044687No Hit
GCGAAGGGATCCCAACAAAATGATTTACATTATAAATTCACATTATGAACAT1500.10490170710044687No Hit
AACTATGGGAGTCTTTCAAAGCCAGCCAGGCTACACACAGAAAATGTTTACT1500.10490170710044687No Hit
TGTAGTGGGGAGGGTTCGCTCGCCGGCTCCGACGCAGACATGGTGGACTGAT1490.10420236238644391No Hit
GATTTGGGGGAATCTGTGAACAAATACTTGAAAATGGGTTCAATGTAGACTG1480.10350301767244091No Hit
GACTCAGGGTAATAGCCCTCCAAATGGGTGTGACGTATTTTGATTCTATGTC1470.10280367295843794No Hit
TACGCAGGGGCTTCTGCTGAGGGGGCAGGCGGAGCTTGAGGAAACCGCAGAT1460.10210432824443497No Hit
AGATGGGAGTCCTCGAAAAAGAAAAAGGGGCTGCTTTCCAAAGGCAAGAAAC1460.10210432824443497No Hit
CGCGCTGGGATTGCCTAAGAATCTGTAAATTTTGTTGCTCAACTATAAAATC1440.10070563881642901No Hit
AAATTGGGGGATGACCTTAAGAAACAAGTAAGCCAGAAGTATAAGTTATATT1440.10070563881642901No Hit
CGACTTGGGATTGGCTGAGAACATAATCTTGCTGCTTTCAAAAGAATCATAA1430.10000629410242602No Hit
CGTTTTGGGGATCTAATATGGGGAAGAATTGTCTTGGGTCACACATAATATA1430.10000629410242602No Hit
CTAGCCGGGAATCCAGTCCAGGTAACTAAAGAAAAAAACTTTTTATATTAAT1430.10000629410242602No Hit
AGCAGCGGGTGGTAGCGCTCAGGGCGTGAGGCTACACTCCTGCCCTGCCTTT1430.10000629410242602No Hit
CTAATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1430.10000629410242602No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)