FastQCFastQC Report
Sat 31 Dec 2022
SRR5844845.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844845.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140766
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTTTGCGATCTCGTATGCCGTCT5340.37935296875665997No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACTTTGCGATCTCGTATGC5170.36727618885242175No Hit
GAGCAGGGCTCCTCAGTCTTTTGGCTGTATTTTGCCCATTGGCTGTTAATGC1610.11437420968131509No Hit
TCGAAAGGGATCTTGAAGAATTTGGAGTTTTTCAGGGCAATTCAAATGACCT1580.11224301322762598No Hit
GTATTGGGGACTGTCACAATGAAAATCTTGACAATTTACTTAACAAGTAACC1580.11224301322762598No Hit
GAAATGGGGACTCTGAATTCAGTGCTGCTTTGGGACATATACTTGACCTAGC1570.11153261440972961No Hit
CAAGTGGGAAGGCTGCAATGAGCCGTGTTCATGTCTGTCTCTGAATAAATAA1570.11153261440972961No Hit
CATCTGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1550.11011181677393689No Hit
ATGCTGGGGTGGAAAAACAGACTATCTCCATTGTGGAATAGTTAAATTAATC1530.10869101913814415No Hit
AACACCGGGGGCGGCCACCGTGGAGAGCAGAGCGCGGCGGCTGGAAGCTGCT1520.10798062032024779No Hit
CGTAAAGGGACCATTACCCAATGTGCCTTTAAATATAGTTTTATTATTTAAT1510.10727022150235142No Hit
TGCCGAGGGTGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1510.10727022150235142No Hit
GCCTAAGGGCGATCTTTCTAGGGGAGGCGGAGCATGTGGTGAGTTGAGCCGC1510.10727022150235142No Hit
CTAAAAGGGGTCCTTTGCCCCTTTTTAATCAATTCCTTTTAATTTGTTATTG1470.10442862623076594No Hit
TTGTCCGGGATTTATACAAAAGTCCCCTAAAAAGGAAACAAGATCCTTTTAA1470.10442862623076594No Hit
AGGGCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAAG1470.10442862623076594No Hit
CTGACCGGGAGAATAATTTGTAAAATTGTAAAATGAGAACAATTTGTGAAAT1440.10229742977707686No Hit
AAATGTGGGGTTCTGATATCTGAGGCCAGTAACTATTAATATCTAGTTCTCA1430.10158703095918048No Hit
CATACAGGGAGCAGCTTGGGAGCTCATTAATGTTCTTGGCAGCCAGGTCCTA1410.10016623332338775No Hit
GCTTCTGGGCGATCTGAGACTGGGGGATAATAAAACCCATTGATCTGTTGCC1410.10016623332338775No Hit
TGTAAGGGGCAACAAAGTAAAGTAGTATTGGATGGTTAACCCAAACTATAAA1410.10016623332338775No Hit
TCTACTGGGGAATCCAGAGATTAAAAACAGAAATATGGAAAAGCTATGTACT1410.10016623332338775No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)