FastQCFastQC Report
Sat 31 Dec 2022
SRR5844831.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844831.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193049
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACACTTTGCGATCTCGTATGC4590.23776346937824075No Hit
CTTATACACATCTCCGAGCCCACGAGACACTTTGCGATCTCGTATGCCGTCT4060.21030929971147225No Hit
TGCTTCGGGACACGAGGCTCAGCAGGCCATGGACATCCACTTCCACAGCCCG2220.11499671067967199No Hit
AGCACTGGGATCTTCATCTGATGGCACTGAAAAGTTACCTGAGCGAGAAGAA2220.11499671067967199No Hit
TGCTCTGGGGAATGAAGTAGATCAGGAAATACTTACTTTGGTATGGTCACAA2120.10981667866707416No Hit
CAACGTAGGGAAGCACTCTGTGTCAACCACCAGATTAGTATAACTATTAATT2120.10981667866707416No Hit
TTTACTGGGAGCTCATTCAGAAGCAGCTCTGAGATTGCAGTTCTGCTACTCC2080.10774466586203502No Hit
TGCACTGGGCTAAAGAGGAGGGAGAAAGTACTTATTTAGCAATATGACTTTT2070.10722666266077524No Hit
TTAACCCGGGCTACGCCGAGTGACAGAGACGCTCAGGCTGTGTTCTCAGGAT2050.10619065625825568No Hit
TGTTAAGGGGTTGGAGCCGGTTAGCGAACCCCAAGAGTGCAGAGTGTGGAGC2030.10515464985573612No Hit
AAAGCAGGGTAACCGAATGTTCATTCATATTCATTTATAAAAAGTACCTGTA2030.10515464985573612No Hit
TTTCATGGGAAATACAAGGGAAAAATATGAATAATGAGTGATTTTTTCCATC2020.10463664665447633No Hit
TCCGAAGGGGATGCCTCCAGTTTTGTTCTTTTTGCTTAGGATTGTCTTGGCT2020.10463664665447633No Hit
TCTAACGGGATAGTGAAGGAGATTCCTAAGCTTTATTTTGAAATGTTAAAAT2020.10463664665447633No Hit
AGCCTCGGGAAACTCCAGATATATATTTCCAACTGCCTGTGCTGTCCGCCAT2000.10360064025195675No Hit
TATGTGGGGCATTGGGTTTACCTACTTATTTGTTTATTTGTTTCTAGATACT1990.10308263705069698No Hit
ACAAATGGGAGATGAAGGCATCCTTGGCATCCCTGCCCCCTCTGCATGTTGC1970.1020466306481774No Hit
AGCTGCGGGTGTGACTTGCTACCTATTGTGGCTACTCCATTAAAAGCAGAAA1970.1020466306481774No Hit
GTCGCAGGGGATGAAAGCTCAAAAGAAGAAACTGAGCCTCAAGTGCTGAAGT1950.10101062424565785No Hit
GTACCTGGGGTCAACCAGGTCCAGATGTGCTATAATCTGTTTTTACGTATGT1940.10049262104439806No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)