FastQCFastQC Report
Sat 31 Dec 2022
SRR5844830.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844830.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences192085
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACACTTTGCGATCTCGTATGC4530.23583309472369005No Hit
CTTATACACATCTCCGAGCCCACGAGACACTTTGCGATCTCGTATGCCGTCT4080.21240596610875395No Hit
GGAATGAGGGGTAGTAATTTATGTGTTTTCTGGCCTCCAAACTGCTACTGCT2300.11973865736522894No Hit
CCTTTTGGGATCTCCTAAAAAGTCACCTTCGATTTGCAACTACTTAGCATTT2220.11557383450035141No Hit
TCTAACGGGGCAGCATGGCTGACCAACTGACTGAAGAGCAGATTGCAGAATT2160.11245021735169326No Hit
GGGTGAGGGCTTGGGAGTGGGCGGGCTCGAGGGCTGGGAGGAAGCCGAAAGC2160.11245021735169326No Hit
AAAGCAGGGTAACCGAATGTTCATTCATATTCATTTATAAAAAGTACCTGTA2150.11192961449358357No Hit
TAGGCTGGGAGGAAGGAGGAGAAAAAGCTTCCTTAAAACTGATTATTTTGAT2080.10828539448681573No Hit
GTCATTGGGATTGACTTCCGAGAGTTCATCTGCGCTCTGTCCATCACCTCCA2070.10776479162870604No Hit
AGCCTCGGGAAACTCCAGATATATATTTCCAACTGCCTGTGCTGTCCGCCAT2000.1041205716219382No Hit
AGCACTGGGATCTTCATCTGATGGCACTGAAAAGTTACCTGAGCGAGAAGAA1990.10359996876382851No Hit
GTTGCCGGGTAAATAAATGACAAAAGAAAGAAAAAAAGGCCAAAAAGTCTAC1990.10359996876382851No Hit
TTTCATGGGAAATACAAGGGAAAAATATGAATAATGAGTGATTTTTTCCATC1980.10307936590571883No Hit
TGTATAGGGAGAGTCTCTTTTCTTAAATATTTTAATATCATTAAGATTTCAC1950.10151755733138976No Hit
TTAAGAGGGATGATGCTCAAAGGAAATACTGGAGTATTTTGCATTTTGGATT1950.10151755733138976No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)