FastQCFastQC Report
Sat 31 Dec 2022
SRR5844828.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844828.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176237
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTTTGCGATCTCGTATGCCGTCT7700.43691165873227533No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACTTTGCGATCTCGTATGC7110.403434012154088No Hit
GTTAGCGGGTTGATAGAGAGGGATATTGAAATCTCGAAATGTAATTGTGCAT2190.12426448475632244No Hit
AAGTTTGGGCACAAACATGAATAGATGTTCAACCACAATGACAATCAGAGAA1890.10724195259792212No Hit
CAACATGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1820.10327002842762871No Hit
CGAACGGGCTACGGCAAGAATGGCTTGGAGGGGCAGGAGTAGAGGAGAGAGA1810.10270261068901536No Hit
GTTCTTGGGATGAGAAACCAAAAAGTATAGTGGAAAAAAAATGATGATTTTT1780.10100035747317533No Hit
ACATATGGGGGAACTTTTCTTCAAATTGCAATCTGCCTTTCCTAACTGCAAC1780.10100035747317533No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)