FastQCFastQC Report
Sat 31 Dec 2022
SRR5844823.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844823.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150904
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTTTGCGATCTCGTATGCCGTCT6440.4267613847214123No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACTTTGCGATCTCGTATGC6010.3982664475428087No Hit
AGCCTGGGTGACAGAGTGAGACTTTGTCTCGAGTAAAAAAAGAGAATGTAAA2300.15241478025764726No Hit
TCAAGGCGGGATGTGAGAAACTGCCGAGTAGGTTCCTTCCTCAAAACTCTGA2140.14181201293537612No Hit
GACACTGGGATTTAACAAAGTGACATTGTGGAGTCATCAGTAAGAATGTGGC2090.13849864814716642No Hit
GCCAATGGGCACGACGCTCTTTCGATCTGTCCTCGGGCAGCTTCAGTGACCC1990.13187191857074698No Hit
AGGTATGGGGAGGCGACGCGCTGTCGGTGGCCGGCTGCGGCTCGTGCCTGGA1960.12988389969782113No Hit
CAACATGGGAGATTTAACCCAAAAGAAGATTACCTCAAGGCATTTAATATTC1960.12988389969782113No Hit
AAACATGGGAAATGAAAGCTACCAATTTAAAGTTACGGAATCTACCATTTTA1960.12988389969782113No Hit
TGAAATGGGTCTGCACGGGATGACCCCAATACGCAAAACTAACCCCCTAATA1950.1292212267401792No Hit
GATCCGGGGATCCGGGGACCTGCAGGTTACAAGCAGCAGATCCCACCTTCAG1900.12590786195196949No Hit
AAAATTGGGGTGAGAGTTTCCTGAGGTACCTCACCCTTCCACAAGACAACAG1890.12524518899432752No Hit
TCGTTTGGGAAAATGATGTGATCATGTGCCCTTCTCAGTGCATCGTATTTCA1880.12458251603668558No Hit
TATTTCGGGAAGACTTCAGTTTTTGACCTCCACAAAGACAGCAATTAGAGAT1860.12325717012140168No Hit
ATTGGTGGGTATTTGTTCACAGATAATATGATCTTATAGGTAGAAAATTCTG1860.12325717012140168No Hit
ATCGCTGGGATCAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAA1850.12259449716375975No Hit
CTTATAGGGACTCGTGTCTCGCTACCAGCTCCCCGCTGCCCTGCGCTCGGCG1810.11994380533319196No Hit
GTCGACGGGTGACTAGAAATCTAAAGGCTAATTCCAAAGAGAGGATTCATTG1800.11928113237555003No Hit
GCCTAGGGTAGGACGCAGCTGTTGCCATGACGGCCCAGGGGGGCCTGGTGGC1790.11861845941790808No Hit
TGTTGCAGGGGCTAGAATTTGTTTTAATTTTTTGTATTTTGAGCCACTTCAC1790.11861845941790808No Hit
AGAGTGGGAGACTACTGTGCTATCTGCTTAAGATGGGAGTGGCCTGGGTCTC1780.11795578646026612No Hit
ATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAG1780.11795578646026612No Hit
CTAAAGGGGCTGCAGAGAGTATTTTGTCTCTCCCAACATTATTACAGAAGTT1770.11729311350262418No Hit
TGTCTGGGGAATCTTCAGCAACTTTGTGGCCCCAAGTGCCGTGACCTGAAAG1770.11729311350262418No Hit
TACTACCGGGGCAATTTAAGGAGCAAAATAAGTGAGAATACATAAAACTAGA1750.1159677675873403No Hit
AAGTGAGGGATTCAAGGTTTTACATTCTATGATTGAAAAAAATTTTTTGAAA1750.1159677675873403No Hit
CGAGTGGGGGGTCTTGTTCTTGGTCTTGTCAAACCTTGCCTGATGCTCTTTC1730.1146424216720564No Hit
ACCGCTGGGATGGCTCTTGTTTATCAGCTAATCTTGAATTTATTCTGTGGTA1720.11397974871441446No Hit
AAAGCCAGCGCTCCCGCCCGCTCCCCGACTTAGGATCCGATGCCGGCAGCGT1710.11331707575677251No Hit
AGCAGAGGGGATATCTCAGCCGAGAGGATGTTATGAGAAACACAGATTTGGG1710.11331707575677251No Hit
TAGTAAGGGGAGATACAAGCAGTAGTTGTAGTTATGTTAGGAATAGAGCTGG1710.11331707575677251No Hit
CTCCAGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1700.11265440279913058No Hit
CGCCAAGGGTCTGTTGGCGGGCAGCAAAGAAATTTATTTTGAGAATATGAAT1700.11265440279913058No Hit
AACGCAGGGGAGGAACAGTCGCCAAGTATTTCAGGTTGCTTCTTAAGTTCTG1690.11199172984148864No Hit
TGAGAAGGGTCTCATATCTGAGTCTTTCTGGAGTGTATTTGGTGTCTGTGTG1680.11132905688384669No Hit
TGATAGAGGGATACTATCTAGTGAATCCTGATTTATGATAGGCCACACTATA1680.11132905688384669No Hit
TGTGCTCGGGAATGCACCCAAGAGGCCTCCTTCGGCCTTCTTCCTCTTCTGC1680.11132905688384669No Hit
CCAACCGGGTGTTGAAGAAATTGAGAATATTTAGGCTGAACAAGAAAAGATC1670.11066638392620473No Hit
TTATATGGGATACTTAATCATATGAAAGTTGGTGAAACTTGGATTACAGATT1670.11066638392620473No Hit
TGTGGGGGTGCAAACATGAAGCAAATAATCACACAAATGTCTAAAGAATTAA1670.11066638392620473No Hit
AGAAGGGGGATGAGGCCTGAATACCGCAGCAAAATAGATGCCAAATACAAAA1670.11066638392620473No Hit
TTCCTAGGGATCCTCCACATGGGAGGTAAATTGTTGAAACCTTGCATGACTG1670.11066638392620473No Hit
TTGTATGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1660.11000371096856279No Hit
GTATTGGGGGGAACTTGGCTCAGCTGCCGGGCTGCTCCGGTTGGAAACGCCA1650.10934103801092086No Hit
TAGATTGGGGATATCAGCTTGAGTAATTAACTCATGATTATGCAAAGAAAAT1650.10934103801092086No Hit
GCTCTGGGCTACCATGAACAGCTTTCTGTAGCAGAGATCACCAATGCTTGCT1640.10867836505327891No Hit
AACACAGGGATGTCAGGTAGGGTTCAGGGACTGATTGGTCCCATTTGCCCTC1640.10867836505327891No Hit
GATGTGGGGCATGACCTTGTGTTAGGCAATTAATTCTTAGATATGACATGAA1640.10867836505327891No Hit
TCTAGCAGGGAATCAAATACTTTTGTGTATAGAAAATATGTATAGTAGGTAG1640.10867836505327891No Hit
CTCTCAGGGTCTGTAATAGAATTCCTATTCATCCTGAAATCATAGCAAATTA1640.10867836505327891No Hit
CACACACAAACTGAGAAAAGATTTGTATCAAACAGAATCAGAGGCATATGAA1640.10867836505327891No Hit
CGTACAGGGCCGATCTACAGTGGGGATTTCTTTAATGTTCTAAACAGCCGTA1630.10801569209563697No Hit
ATTATTGGGACATGAAAGTAGATAGCTTTCTCATATCTCACAACAAAAATAA1630.10801569209563697No Hit
AAGCATGGGGAACATAATTTGTTAAATTCTCAAGCAAGGTTTTCACTTTTAT1630.10801569209563697No Hit
GCAGCTGGGCAGAGAGCACAGGGGTGGGGAGCAGCTCTGCAGCAGAGAAACT1620.10735301913799501No Hit
GATCTGGGGCCAGAGGGATGGCAACTTTGAAAGATATCACCAGGAGACTAAA1610.10669034618035307No Hit
ATTGGGACTTGTATGGTGATGATCGAATTGAGCCATACATTCATGAAGGAGA1600.10602767322271114No Hit
GATACAGGGAACCTGGGTTGACTAGATGTTCTTTTTTGCATTTTTCTAGAAG1600.10602767322271114No Hit
ATACCGGGATCTTTGAAGCGGGGAAGTACAGCTGCTGGGATCAGCCAAGACT1600.10602767322271114No Hit
TCTAAAGGGAGGAAATAAGTTAAACAATGCTGCAAACAAATTTGGTAACCCT1590.10536500026506919No Hit
CGCGCAGGGCGAAGCGTGGGAGGCACCCCCATTGCTCTCCTCCAGGGGCAGA1590.10536500026506919No Hit
GGGTGGGGAAGGTCAGGGTAGAAGGAAACATGTTTTCCCCAGCTCCCAGGAG1590.10536500026506919No Hit
ATAGTTGGGATGGCTAAGTTGAGTTAATTAACATATTTATTGTCTCACATAC1590.10536500026506919No Hit
TAGAGTGGGGGAGAGTCGGACTAAATGTGCGCCTTAAGTAACTGAGAATCTC1590.10536500026506919No Hit
GTTAAGGGGAGAAAGAGAGAGAAGGAAAGGAGAGAAGGCAGGAAGAAGGCAA1590.10536500026506919No Hit
CTTTGATGGGGCCGATCTAATTCAGGGTGGACCAGACAACTGCCGGCTGGTC1590.10536500026506919No Hit
ACTGGTGGGATCACAAAGCTATTGGTAATTAAAAAAAGAATTAGTATCAACT1590.10536500026506919No Hit
GCCTTAGGGGGCAGACACTGACCTTCAGCGCCTCGGCTCCAGCGCCATGGCG1580.10470232730742723No Hit
GCTTCTGGGAGATACTGAGCAAATAAAGAGGGTATACACGTCCAGAACATTT1580.10470232730742723No Hit
GCAGGCTGGGACAATGAAAAGAAAATAGCTATTTTACATGAAAATTTTACAA1580.10470232730742723No Hit
GATGCCGGGGTGCAGCAGAACAACACAGAGTCCTTTGCTGTCGCACTCTGCC1570.1040396543497853No Hit
CATCGCGGGGTTGGAGGAAGCAGCGGCAGCGGCGAGGGCGGCAGGCTAGCTG1570.1040396543497853No Hit
TTTTTAGGGATAATGAAAAATATCACTAGATGCCTACTTCCTGTGGACTAGC1570.1040396543497853No Hit
CACTCGGGCTACCAGAAGCTATTTCATTTATTCAGAATAATAAGGAACTTGG1560.10337698139214334No Hit
GCGTAAGGGCGTCTGTATAATCGGCTTCTGGAGTGAAACAGCAAACCCCAAA1560.10337698139214334No Hit
TGATGTGGGAACTGTGCCCAAACTGATCTATAGATTTAATATAATTCCTATT1560.10337698139214334No Hit
TTTTATGGGGAGTTGTAAATCAAGTAAAATTATTGTGACTGTGTGTATATGC1560.10337698139214334No Hit
GTAGGCGGGAGTGGGAGGACAGATTCCAAAGCAAAACTGGGTTGCTCCTTCC1560.10337698139214334No Hit
GTGTCAGGGGCCTGACCAATATGATGAAACCCCACCTCTGCTAAAAATACAA1560.10337698139214334No Hit
GCAAACGGGACTGGCTTTCTTTTTTCCTGTTTTCTTATGCTTCCAAAATCTT1560.10337698139214334No Hit
AAAGGTGGGTTGCTCTGGCAAGTAATGAAAGCGTTCTCGCTTCCTGAGTGTG1560.10337698139214334No Hit
GTAATAGGGCAGGATGATAAAAAAGAAGTCCATCCCCAAGACACATAATCAT1560.10337698139214334No Hit
GTGCATGGGAGATACCAGAAATCTGGGAAGGCCATAATATAGCTGATTATAT1550.10271430843450141No Hit
CTAACTGGGCGATCTAAAGCAGGGATTGGATTTGGATATTTGAGGTAAAATT1550.10271430843450141No Hit
CAGGCTGGGAACAAAAGTATGGCTTAACATTCCTAAAGCTTTGATTTGTTGC1550.10271430843450141No Hit
CGATCTGGGGTAAAGTTAATGAACAATGTAGACAGCTGATTAAGAAAAAAAA1550.10271430843450141No Hit
CGAAACGGGCACGTCACTGCACTCTAGCCTGGGCAACAGAGTAAGACAAAAA1540.10205163547685947No Hit
TAACGCGGGGACAGGCAGGGTGATAGTAACCTTGGAGGAGAAAAGATTTCAA1540.10205163547685947No Hit
TCAAAAGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAA1540.10205163547685947No Hit
ATTTGGGGGAGTAGACCTTTAATGGAGACATGCATTTGCTAACTCATGGAAC1540.10205163547685947No Hit
TAGCGCGGGATCTTTGTTTGAAACATCGCAGGATAATGATACTTATTGAAAT1540.10205163547685947No Hit
AGAACTGGGAGAAGAAATTTGGGGAATCTTAGTTGACAATATTTAGGCAATT1540.10205163547685947No Hit
AATGCAGGGTCCTCTTGCTTCCCAAAGATTTTAAGCATTTTGTAAATGTATA1530.10138896251921752No Hit
GAATTGGGGAGACAAGGAACTGACTTGCGAATTCTGAGTTGCTTTCAGACTC1530.10138896251921752No Hit
TTACTGGGAGGTATATACAACTGTGTTATATATGCAGGTGGATACTCACGTG1530.10138896251921752No Hit
CATTTTGGGAGTACTAAGTATTTTTCTTGATTGGTCTTACAGCTGGGGAGAA1530.10138896251921752No Hit
TCAGTAGGGGAACAGTAATAAGCCGTCCTGCACCTGTCCTTGGCTCCAAGGC1530.10138896251921752No Hit
CAATTTGGGGGTTTCCCTTGGGAATATTAATCTCTTCGATCAGATTGAAGAC1530.10138896251921752No Hit
TTTGACGGGAAAACAAGTTGCCTGTGTGTTTTTTTATTTTTTACTTATCAAA1520.10072628956157557No Hit
GCATAGGGGAGAGCATTTGCCTAACTGTGAAAGGGTTGTATGGAAAAATAAT1520.10072628956157557No Hit
TCGCATGGGAAACTGACCTCTGTGTCTTTTTCACATGTCCCCATTAGACTTT1520.10072628956157557No Hit
AGTACTGGGACATCTAAGAGAAAAATGGAAAAACACACATGGCAGGGAAGCC1520.10072628956157557No Hit
AGTTCTGGGATGGGATGCTGTGTCACAGGGAAATTTATTATAGCCACATACA1520.10072628956157557No Hit
ATAGCTGGGTATAATACGCCTCACACTCATTCTCAACCCCCTGACAAAACAC1520.10072628956157557No Hit
TTCGAGGGGATGGTAAACTTTGTGTTGCTCTTTATGAAATGATCCATATAAC1520.10072628956157557No Hit
AAGCATGGGATTGGTTTTTGGAAGCACTACTAATTTTTAAATGATGTGGTAA1520.10072628956157557No Hit
TAAGCAGGGGATGGCGCGATAAGTGTAGCAGAGATGAGGTAAAAGACGGTGA1520.10072628956157557No Hit
ATCAGAGGGGTTTTTGAAGAGATAAACAAATTTGCAGATGAAAAATGAAATT1510.10006361660393362No Hit
CTATATGGGAATGGCACAATTGACTTCCCTGAATTTCTGACAATGATGGCAA1510.10006361660393362No Hit
GCCAGGTCCTGTTGGAGCAGTTTGCTTCCGTCAGAGGGTCTTTGGATTCTCT1510.10006361660393362No Hit
CGAGAAGGGGCAATTCCTTGGATTTATCTGACAAAAATAAATCTAATTTTTA1510.10006361660393362No Hit

[OK]Adapter Content

Adapter graph