FastQCFastQC Report
Sat 31 Dec 2022
SRR5844821.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844821.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149233
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTTTGCGATCTCGTATGCCGTCT6470.4335502201255754No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACTTTGCGATCTCGTATGC5660.37927268097538747No Hit
TCAAGGCGGGATGTGAGAAACTGCCGAGTAGGTTCCTTCCTCAAAACTCTGA2280.15278122131164018No Hit
TAGAGTGGGGGAGAGTCGGACTAAATGTGCGCCTTAAGTAACTGAGAATCTC1990.13334852210972103No Hit
AAGCATGGGGAACATAATTTGTTAAATTCTCAAGCAAGGTTTTCACTTTTAT1960.1313382428819363No Hit
AGAGTGGGAGACTACTGTGCTATCTGCTTAAGATGGGAGTGGCCTGGGTCTC1940.1299980567300798No Hit
GCGTAAGGGCGTCTGTATAATCGGCTTCTGGAGTGAAACAGCAAACCCCAAA1920.12865787057822334No Hit
TGTCTGGGGAATCTTCAGCAACTTTGTGGCCCCAAGTGCCGTGACCTGAAAG1920.12865787057822334No Hit
GCTCTGGGCTACCATGAACAGCTTTCTGTAGCAGAGATCACCAATGCTTGCT1910.12798777750229506No Hit
ACCGCTGGGATGGCTCTTGTTTATCAGCTAATCTTGAATTTATTCTGTGGTA1900.12731768442636682No Hit
AGGTATGGGGAGGCGACGCGCTGTCGGTGGCCGGCTGCGGCTCGTGCCTGGA1890.12664759135043857No Hit
TGAAATGGGTCTGCACGGGATGACCCCAATACGCAAAACTAACCCCCTAATA1880.12597749827451032No Hit
TTCCTAGGGATCCTCCACATGGGAGGTAAATTGTTGAAACCTTGCATGACTG1880.12597749827451032No Hit
TATCATGGGCAATGGCCCCTAAATATTCAAGTCAAAAGACGGGTCACAGGTC1860.12463731212265385No Hit
AGCCTGGGTGACAGAGTGAGACTTTGTCTCGAGTAAAAAAAGAGAATGTAAA1850.1239672190467256No Hit
GCAGCTGGGCAGAGAGCACAGGGGTGGGGAGCAGCTCTGCAGCAGAGAAACT1850.1239672190467256No Hit
GCCAATGGGCACGACGCTCTTTCGATCTGTCCTCGGGCAGCTTCAGTGACCC1850.1239672190467256No Hit
TGTTGCAGGGGCTAGAATTTGTTTTAATTTTTTGTATTTTGAGCCACTTCAC1850.1239672190467256No Hit
ATTGGTGGGTATTTGTTCACAGATAATATGATCTTATAGGTAGAAAATTCTG1850.1239672190467256No Hit
AAAGCCAGCGCTCCCGCCCGCTCCCCGACTTAGGATCCGATGCCGGCAGCGT1840.12329712597079735No Hit
CTATATGGGAATGGCACAATTGACTTCCCTGAATTTCTGACAATGATGGCAA1830.12262703289486909No Hit
CTTATAGGGACTCGTGTCTCGCTACCAGCTCCCCGCTGCCCTGCGCTCGGCG1830.12262703289486909No Hit
TCTACAGGGAAGTTGACACCTGATAACTTCTCCCTGATGGGTTTCCGAACTG1820.12195693981894085No Hit
ATAGTGGGCTATTGTTTAAAGTGATTACATTTAACGTCCTCTCCCATGACTT1810.1212868467430126No Hit
AGCAGAGGGGATATCTCAGCCGAGAGGATGTTATGAGAAACACAGATTTGGG1800.12061675366708435No Hit
CAGGCTGGGAACAAAAGTATGGCTTAACATTCCTAAAGCTTTGATTTGTTGC1790.1199466605911561No Hit
TACTACCGGGGCAATTTAAGGAGCAAAATAAGTGAGAATACATAAAACTAGA1780.11927656751522786No Hit
CAACATGGGAGATTTAACCCAAAAGAAGATTACCTCAAGGCATTTAATATTC1780.11927656751522786No Hit
GATACAGGGAACCTGGGTTGACTAGATGTTCTTTTTTGCATTTTTCTAGAAG1780.11927656751522786No Hit
GTCGACGGGTGACTAGAAATCTAAAGGCTAATTCCAAAGAGAGGATTCATTG1780.11927656751522786No Hit
CCAACCGGGTGTTGAAGAAATTGAGAATATTTAGGCTGAACAAGAAAAGATC1770.11860647443929961No Hit
TAAAAGGGATCCTATGACAAGAAAAAACAGTCTGAAGAGAGAAAGCAATTAG1770.11860647443929961No Hit
CGCCAAGGGTCTGTTGGCGGGCAGCAAAGAAATTTATTTTGAGAATATGAAT1770.11860647443929961No Hit
CACTTGGGGAAGATAGCAATCAATCCTTTAAACATACTTGTAAACCTCAGTT1760.11793638136337137No Hit
CTTTGATGGGGCCGATCTAATTCAGGGTGGACCAGACAACTGCCGGCTGGTC1760.11793638136337137No Hit
TAAGCGAGGGCAACAGAAGACAATTTTTAAAAGTGAAAAAATAAGCCACAAA1750.11726628828744312No Hit
ATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAG1750.11726628828744312No Hit
TTATATGGGATACTTAATCATATGAAAGTTGGTGAAACTTGGATTACAGATT1740.11659619521151487No Hit
TACGCAGGGGGAGCCGCGAGGGACAGCAGGCCCAGCCCTCAGCACCACCTGC1730.11592610213558663No Hit
TTTTTAGGGATAATGAAAAATATCACTAGATGCCTACTTCCTGTGGACTAGC1710.11458591598373014No Hit
GTAGGCGGGAGTGGGAGGACAGATTCCAAAGCAAAACTGGGTTGCTCCTTCC1690.11324572983187364No Hit
TGATAGAGGGATACTATCTAGTGAATCCTGATTTATGATAGGCCACACTATA1690.11324572983187364No Hit
TGTAATGGGAGCTTAACAAAAAATATAGAAAGAGAAAAAGGGAATTTTAAAA1690.11324572983187364No Hit
CTATCAGGGACGCTGCTGATAGAGATGTACCCGAGACTGGGCAATTTACAAA1680.1125756367559454No Hit
AAACATGGGAAATGAAAGCTACCAATTTAAAGTTACGGAATCTACCATTTTA1680.1125756367559454No Hit
CATCGCGGGGTTGGAGGAAGCAGCGGCAGCGGCGAGGGCGGCAGGCTAGCTG1680.1125756367559454No Hit
GCCAGGTCCTGTTGGAGCAGTTTGCTTCCGTCAGAGGGTCTTTGGATTCTCT1670.11190554368001716No Hit
TTGTATGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1670.11190554368001716No Hit
ATTGGGACTTGTATGGTGATGATCGAATTGAGCCATACATTCATGAAGGAGA1660.11123545060408892No Hit
GCCTAGGGTAGGACGCAGCTGTTGCCATGACGGCCCAGGGGGGCCTGGTGGC1660.11123545060408892No Hit
AAAATGGGGAAATGTATCTGGGGTTCCCTGAAATTTGAAAGACTGTCGGTCT1660.11123545060408892No Hit
GTCTTGGGGTAACAATTACCTCTCTACGTGATGCTGTAAGGAATCTTGCTAA1650.11056535752816067No Hit
ACAATGGGGGAGTACAAAAGCAGTAATTAAAATTTTAAATGTCACTTAACTT1650.11056535752816067No Hit
TTTTATGGGGAGTTGTAAATCAAGTAAAATTATTGTGACTGTGTGTATATGC1650.11056535752816067No Hit
TAACGCGGGGACAGGCAGGGTGATAGTAACCTTGGAGGAGAAAAGATTTCAA1650.11056535752816067No Hit
CTCTCAGGGTCTGTAATAGAATTCCTATTCATCCTGAAATCATAGCAAATTA1650.11056535752816067No Hit
GACACTGGGATTTAACAAAGTGACATTGTGGAGTCATCAGTAAGAATGTGGC1640.10989526445223242No Hit
CACTCGGGCTACCAGAAGCTATTTCATTTATTCAGAATAATAAGGAACTTGG1630.10922517137630418No Hit
CTAAAGGGGCTGCAGAGAGTATTTTGTCTCTCCCAACATTATTACAGAAGTT1630.10922517137630418No Hit
TATTTCGGGAAGACTTCAGTTTTTGACCTCCACAAAGACAGCAATTAGAGAT1630.10922517137630418No Hit
GCTTTTGGGCCAGGGTAGCATCACGCCCACAGACTACACCCTCTCCAAGTGC1630.10922517137630418No Hit
GCATAGGGGGAAATGGTACCCCTTGTTCAAAATGAACAAGAAGCCTTAGATT1620.10855507830037593No Hit
AGTTCTGGGATGGGATGCTGTGTCACAGGGAAATTTATTATAGCCACATACA1620.10855507830037593No Hit
ATGACTGGGATGCTGGGATCTCAGGGACTGAGGGAGGTGTGACACAAGTAAT1620.10855507830037593No Hit
TACATCAGGGCCACTTATAAGTGAGAACATATGGTATTTGGGTTTCTGTTCC1610.10788498522444769No Hit
AAGTGAGGGATTCAAGGTTTTACATTCTATGATTGAAAAAAATTTTTTGAAA1610.10788498522444769No Hit
AACATCGGGTTAAGAGAGAAGAATTTTTAATGAACAAAACTGTAATATGGAT1610.10788498522444769No Hit
TAGATTGGGGATATCAGCTTGAGTAATTAACTCATGATTATGCAAAGAAAAT1600.10721489214851944No Hit
ACTGGTGGGATCACAAAGCTATTGGTAATTAAAAAAAGAATTAGTATCAACT1600.10721489214851944No Hit
TGAGAAGGGTCTCATATCTGAGTCTTTCTGGAGTGTATTTGGTGTCTGTGTG1590.10654479907259119No Hit
AGAAGTGGGACAGAAAGATCACCTGCTGCATACGCCAGACACAGAAAGATCA1590.10654479907259119No Hit
AGGGTGGGGAAGGGAATTTCTTCAAAAGGTATAAGTTAGTTTGCAAATAATA1590.10654479907259119No Hit
TGTAAGGGGCCCAATTGTAAATTTAATGTGATTTTGACTGTTTTATTCACCA1590.10654479907259119No Hit
GCAAACGGGACTGGCTTTCTTTTTTCCTGTTTTCTTATGCTTCCAAAATCTT1590.10654479907259119No Hit
TAGCGCGGGATCTTTGTTTGAAACATCGCAGGATAATGATACTTATTGAAAT1590.10654479907259119No Hit
CGAGTGGGGGGTCTTGTTCTTGGTCTTGTCAAACCTTGCCTGATGCTCTTTC1580.10587470599666293No Hit
GATGCCGGGGTGCAGCAGAACAACACAGAGTCCTTTGCTGTCGCACTCTGCC1580.10587470599666293No Hit
AACTACGGGAGTGGAATGCAATCCACTCCCTGCTTTTATCTATACCAGGTTA1570.10520461292073469No Hit
CGTACAGGGCCGATCTACAGTGGGGATTTCTTTAATGTTCTAAACAGCCGTA1570.10520461292073469No Hit
TCGTTTGGGAAAATGATGTGATCATGTGCCCTTCTCAGTGCATCGTATTTCA1570.10520461292073469No Hit
CAATTTGGGGGTTTCCCTTGGGAATATTAATCTCTTCGATCAGATTGAAGAC1570.10520461292073469No Hit
AAAATTGGGGTGAGAGTTTCCTGAGGTACCTCACCCTTCCACAAGACAACAG1560.10453451984480644No Hit
GAATTGGGGAGACAAGGAACTGACTTGCGAATTCTGAGTTGCTTTCAGACTC1560.10453451984480644No Hit
ACATACGGGGGGGATATTAGAAGACACCAAAATAGCATTGATACAATCAACA1560.10453451984480644No Hit
ATTATTGGGACATGAAAGTAGATAGCTTTCTCATATCTCACAACAAAAATAA1560.10453451984480644No Hit
GCTTCGGGCTATGGAATTATTTTCAAACATTGTTCCTCGGACTGCTGAGAAC1560.10453451984480644No Hit
TAGTAAGGGGAGATACAAGCAGTAGTTGTAGTTATGTTAGGAATAGAGCTGG1560.10453451984480644No Hit
ATCGCTGGGATCAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAA1560.10453451984480644No Hit
GTGCATGGGAGATACCAGAAATCTGGGAAGGCCATAATATAGCTGATTATAT1550.10386442676887819No Hit
GATGTGGGGCATGACCTTGTGTTAGGCAATTAATTCTTAGATATGACATGAA1550.10386442676887819No Hit
TGCTATGGGCAGTGTCAAGATATACATGAGGTAAAAGTTGATAAAACTGAAA1550.10386442676887819No Hit
GATCCGGGGATCCGGGGACCTGCAGGTTACAAGCAGCAGATCCCACCTTCAG1550.10386442676887819No Hit
CAACGTGGGCTTACAAAAATCGATGGAAGATGCCCCCCAACTTGTGCCTGTC1550.10386442676887819No Hit
TGTGGGGGTGCAAACATGAAGCAAATAATCACACAAATGTCTAAAGAATTAA1540.10319433369294995No Hit
CGATAAGGGTCTTGTCTCAATGCTCATAACGGATCAGAAATACAGATTTTGA1540.10319433369294995No Hit
GCAGCGGGTTATTACATGTTTCAGATTTATGCAACTTTATTACCCTTAGGTT1540.10319433369294995No Hit
ACTTATGGGACTCAGGCTTTAACTAATTATGGAAGTGGAGAAGATGAAAATG1530.1025242406170217No Hit
AGAACTGGGAGAAGAAATTTGGGGAATCTTAGTTGACAATATTTAGGCAATT1530.1025242406170217No Hit
GCAGGCTGGGACAATGAAAAGAAAATAGCTATTTTACATGAAAATTTTACAA1530.1025242406170217No Hit
GTTAAGGGGAGAAAGAGAGAGAAGGAAAGGAGAGAAGGCAGGAAGAAGGCAA1530.1025242406170217No Hit
TTTCGCGGGGGCCCAGAATCTTGAGCAAGCTAAAGAAACCATCATAATCTAA1520.10185414754109345No Hit
CTCGCAGGGAGGAGATGATCGTGATTCATGCAGTGGGCTCCATGTAATCACA1520.10185414754109345No Hit
GACTACGGGTGTGGAAGAGCTGGGAGCTGCTGAAGGAAGGTTTTCTTTTGGC1520.10185414754109345No Hit
AGAAGGGGGATGAGGCCTGAATACCGCAGCAAAATAGATGCCAAATACAAAA1520.10185414754109345No Hit
ACTGCAGGGTGCAGTCGGGGGCAGAGGCAGGCGGGAGGACCGCCTGCCCCCA1520.10185414754109345No Hit
CTTTTAAGGGAATGTTACCAATCAGTGAGCAAGCAAGCACAGCCAAAATCGG1520.10185414754109345No Hit
TGCTACGGGAAGAAAATTAAGTTTGTTAAATTATAATTATGAGACTTTCGTT1510.10118405446516521No Hit
TCTAAAGGGAGGAAATAAGTTAAACAATGCTGCAAACAAATTTGGTAACCCT1510.10118405446516521No Hit
TCTGTCGGGGTGAGAGAAGTAAGACCTAAATAACTAACTGTTCATGGATTGG1510.10118405446516521No Hit
TCTAGCAGGGAATCAAATACTTTTGTGTATAGAAAATATGTATAGTAGGTAG1510.10118405446516521No Hit
AGTGACGGGAACACCTTGCACGACCAAACGAACGAAGATGACCAGAGTACTC1510.10118405446516521No Hit
GCTTCTGGGAGATACTGAGCAAATAAAGAGGGTATACACGTCCAGAACATTT1510.10118405446516521No Hit
AAGAATGGGGTGAGGGCTCCTCTGCATCACCTACCAGGATGTCTGGAGGAGA1510.10118405446516521No Hit
CGATCTGGGGTAAAGTTAATGAACAATGTAGACAGCTGATTAAGAAAAAAAA1510.10118405446516521No Hit
CGCTACGGGATTGCACATTTGTGACAGTAGACATTCACTTTCAACTGAAGTC1510.10118405446516521No Hit
TATACAGGGATATCACACTATAGTTATTTTTTCTTTCTTGTAGAATCTACTA1500.10051396138923696No Hit
CTAACTGGGCGATCTAAAGCAGGGATTGGATTTGGATATTTGAGGTAAAATT1500.10051396138923696No Hit
GCGCGGGCATCCGGCAAGTCGATGTCGTAATAACTTGCACAGGAAATAAGAA1500.10051396138923696No Hit
GTTTTAGGGCAACCGGGCACTCCAAGGGATGATGGCGAAATGGGGGGAAATT1500.10051396138923696No Hit
ATAGTTGGGATGGCTAAGTTGAGTTAATTAACATATTTATTGTCTCACATAC1500.10051396138923696No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)