FastQCFastQC Report
Sat 31 Dec 2022
SRR5844797.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844797.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92325
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACACTTTGCGATCTCGTATGC4370.47332791768210125No Hit
CTTATACACATCTCCGAGCCCACGAGACACTTTGCGATCTCGTATGCCGTCT4050.4386677497969131No Hit
ACATGCGGGAAATAGTCAGTGGGCCGAAGAGGAAATCAAAAGAGAAATAAGA1710.1852152721364744No Hit
CGTCTTGGGACCTCAGATTCTTCATTGAGGATTGGTATAGCCATGATTTCAG1480.1603032764689954No Hit
CTACGTGGGTTAGAAATGATTTTTTGATTTCTCTTTTCTCCAAATCCCTTGA1470.15922014622258326No Hit
TGCTCAGGGAGACACGGCCCGAGCGCCGCCGCGGACGCGGACAGAGACCCGC1460.15813701597617114No Hit
CTGCAAACGGGCGAAGCTTAAGGGAAATTACAAAGGAAAAGATTGATAGGTT1450.157053885729759No Hit
CGAAACGGGTTCCACACAGCCCTTGGTCTTAGGCGAGATGATACATTTTCTG1410.15272136474411047No Hit
GTTGCAGGAGGATTCTGGATGTATACAGATGCTCTTCATCTTAGGAAGGGGT1370.14838884375846195No Hit
ATGTTTGGGACTAAAAAAGATGTACACAAAGTATCTTACAGAAAAAGGAAAA1360.14730571351204982No Hit
CATCTTGGGAGCCTTTTCAACGAATAGTATTGGGACTACTGGATATCCACAT1340.14513945301922557No Hit
GCTTGCGGGATGAGCAGAGGGAGCGCTTCTTGCTCTTCTGCGTCCACGGAGA1310.14189006227998915No Hit
AGCCTCGGGGATGCTGATGCATTTTGATGCTTTGTGCATTCATTTCTTTCTG1310.14189006227998915No Hit
TACCCCATGCTTAGAAATTAAATTGGTCCCCAGGCACACCCTTTCATGCCTC1290.13972380178716493No Hit
TATTCTGGGGACGCTCTTCCGATCTTGCAAGCTGGCCATCCGTGAATCCATC1260.1364744110479285No Hit
CCTCAAGGGACATTTTCATTGCCTGAAAAATTAGCCAACGCTTGGGGGGAAA1260.1364744110479285No Hit
GGGCACGGGCCAATAAAGCTAAAACTCACCTGAGTTGTAAAAAACTCCAGTT1250.13539128080151638No Hit
CTTGCGGGCTTTGGAAATGAGTGCACAAATCCAATGAAGCAAAAAGAGCCTT1250.13539128080151638No Hit
TCATCGGGCCACAAGCCAAAGGATGGACTGGGGTTACCTGAAGCTGGAAGAG1250.13539128080151638No Hit
GTACGAGGGTTCATAATTTGCACCAAGTACAGAATAGGATAATGTAGCTTTA1250.13539128080151638No Hit
GACTCTGGGAGCGTGGATTCCCCGGAACAGCTGTCTGGAATAAGGATGATTA1240.13430815055510426No Hit
TCACTCTGGGCAAACATGCTCAGAACACTTACCTCAGCCTACAGTTGGGCAA1240.13430815055510426No Hit
TCTCTTGGGTACTTACTGCCTAAAAACTACAAAACCAGGCTAAGAAATACCA1240.13430815055510426No Hit
CACATAGGGAACGTGCTCCACAACACTCAGACTCTCCTAGAACACACAGAAT1220.13214189006228No Hit
AATTTTGGGGTGAGGAACTGCTGGTCTTGGATTTTTTTTCCATTAAATTCAG1210.13105875981586787No Hit
ACCAATGGGACATCTTTTTATAAATCTTTTTTCTTCCAGTTTTTTTCTTAGA1210.13105875981586787No Hit
GCTAAAGGGAGGGATTGGGTTTAACATACGACTTTTGAGGGAACACATCTTC1200.12997562956945571No Hit
TGTAACGGGGATTTTTGACTCTACATTCTTTATCTACCATACTGCTCTTTTC1200.12997562956945571No Hit
TGTCTTGGGATGAAGTTCAAGGCAAAATAGTATTTTCTATTACTGTGCAGGG1190.12889249932304359No Hit
TTCGCAGGGATATGATATCAAACAAAATAGACTTTAAGTCAAAACTTGTTAC1190.12889249932304359No Hit
GCCCTGGGGTAAAAATTCAAAACAATTGAAATTATATCAAGTATCATCTTTG1190.12889249932304359No Hit
TGAGCGGGAGAATATTGGAGCTATGTCATCTTATCTTTTAATTATTAAAACA1180.12780936907663146No Hit
AAGTTGGGATGCTTTTCCTATGTTGTAAATTTACAGCAAAAATAAAGAAGGC1170.12672623883021933No Hit
GGCTATGGGCTTCCTGTAGCCTAGCTACTGGCTTCTGCCCTTACTGAGGGCA1170.12672623883021933No Hit
ATGGCTGGGGTAACATCAGTCCTAACACCTAAACCAAGTTTTGGGTAAGGGG1170.12672623883021933No Hit
TGGACGGGACACCAAGAAGTGGGCAAAGAGCAACAAAAGTGTTAGGAGCGGA1170.12672623883021933No Hit
CAAGCGGGGGGCTATATAAAATAATGGCTTCCTGGATAACTGTGTATGTATT1160.1256431085838072No Hit
TACATAGGGCAGTCTGAAGTTTTTTGTTAGAAAACTACTATTTTAAACCTCA1150.12455997833739507No Hit
AATATAGGGATGTCTACCCCAGTTGGAAGTATCTCTGCAATGACTGGAAAGT1140.12347684809098293No Hit
TTTTGTGGGAGAGGATTCAGACTCAGAAGTAAGTGAATTTTTTGATAGTTTT1140.12347684809098293No Hit
GCATATGGGGAGTCGTGGACTCGTGCAGCTGGGGCGTCCGCAGCCGCTCGTC1130.12239371784457082No Hit
GTGCATGGGAAGGACAGACACAACGGCAACATTATGCTGGACAAGAAGGGTC1130.12239371784457082No Hit
CATCCCGGGCGACGGCGCCGCTCGCAGGTCGGGTCCGGCTTCCGCCCAGACT1130.12239371784457082No Hit
TCAGTGGGGATTGGATACAAAGGCTAAGGTGACGTGGGTAACTGAAATTCCA1120.12131058759815869No Hit
TTTACGTGGGGTGCTTTCTTCCTTTGCATGATTTACACCTCCGCCTGTTTTG1120.12131058759815869No Hit
TGACGTGGGCTTGGGCTCTCAATGCAATAGAACATTGACATGAGGCCAAAAG1110.12022745735174654No Hit
ATCAGGGAACCTTTTCCTCAAAAAGAACCAAGAAGGGAAACAAGTCCACTTG1110.12022745735174654No Hit
TTTCTGGGATTATGGGGAAAGTGATGCCAGCAGTTCCTTTTCATTATTCTAT1110.12022745735174654No Hit
TAGGTTGGGATCGAGCTAAAGGAAAGCCTGATGCAGCAAAAAAGGGAGTTGT1110.12022745735174654No Hit
GTATACGGGCTCTCTCTCTTTCACTGCAAGGCGGCGGCAGGAGAGGTTGTGG1110.12022745735174654No Hit
ATGAACGGGACATTTCTAAGACATTAAATCCCATTTAAATTCTGTGTGAACA1100.11914432710533442No Hit
TCCGTTGGGCAGTGCCTGCCACCATGCTTGGCTAATTTGTTTTATTTTTATT1100.11914432710533442No Hit
TTTGTAGGGATTACATTCTGACAGGGTGTGTGGCATTGTGTTCTATGCCAGA1080.11697806661251015No Hit
TGAGCATGGGAGGACTAAGACGTAGTGGACAAATTGAAGGTGTACGGCAAAT1080.11697806661251015No Hit
ATTGCAGGGATTCACAATTGCTTCAAAGAGAATAAAATACCTAGGAATCCAA1080.11697806661251015No Hit
TCAAGGCGGGATAGGTATGTTGTGTGTTCTGTGTTCTTGCAGCCTATACGAT1070.11589493636609803No Hit
ATGCGGGGTCACCGCAGGATTTCCGGTAGGTTGGATTGTGGGGTTGGTGTTT1070.11589493636609803No Hit
ACTACGGGGAAAGTTAGTGTGTGTTATGTTTAAGGGAAAGGACAATGATTTC1060.11481180611968589No Hit
GTTGAGGGGTAATTCAAAGAGCAAATTATAGAAACTACAATGACATTTAATG1060.11481180611968589No Hit
TATGCTGGGACCAACCCACTGCCCATGGCATGTCTTTGGGAGGTGTCTGTGA1060.11481180611968589No Hit
GAAAATGGGAGACTTCAGAGAGAGAGCAGGTTTGTAAAATGTTTCTCATCAG1060.11481180611968589No Hit
GTAAAGGGGGAAACGCTGAACTATGCTAATCTGGAATTTGTTTTTATTTGCC1060.11481180611968589No Hit
ATTTAAGGCGATCTTACGGGGGGACTCTTAAGTATGCAGGCTCTAATTTTCC1050.11372867587327376No Hit
TTTTCCGGGTGGCCAGTTAACCACTGGGAGAGCATCCGGACAGACGTTTCGC1050.11372867587327376No Hit
TGATGTGGGCATGAAGAGTAGTCTTTCTATGAATAAATGAGAAGATGCACAC1050.11372867587327376No Hit
CCAACTGGGGAGATATTGGCATTCTGTATTATAGCTGAATTATATTGGATTT1050.11372867587327376No Hit
TATGTCGGGCATTTATAGCACTTATCACATTCTGTCTTTGAATTTTGTATTA1050.11372867587327376No Hit
CATACGGGAGAGTTGTAGAAAATCTCAGATGAGCTTTAAAAAATTTGCTTTT1040.11264554562686163No Hit
CTCTGTGGGATGACGTGTATGTTTTACATGATGTGTGCCTCTTCACGCAGTA1040.11264554562686163No Hit
CCCCCAGCCTCAGAGTCCCCTCGTTTCTCCTTGGAGGCGCTGACGGGTCCAG1040.11264554562686163No Hit
TTCTGGGGGATCACTTAAAAGTTGGACCAGAAAAGTATAGAAAGAGCATAAT1040.11264554562686163No Hit
GTCATTGGGAAGTAGAGACGGGGTCTCACCGTGTTGGCCAGGCTGGTCTCGA1030.11156241538044949No Hit
TCAGTTGGGATTTTACAGGTGAGCAAACTGAGTCTTAAATGGGCTCCACAGC1030.11156241538044949No Hit
GCACTAGGGAGCCTCCTGCTATTTGGCTTTGCAGAGCAGAAGCAGCTGCTGG1030.11156241538044949No Hit
TCGCCAGGGATCGACGTGCAGGTGGGGCTCCTGTACGAAGAGGGTGTTCGCA1020.11047928513403737No Hit
ATCAGAGGGAGAAATGTAATTAATAAACAATGCTCACTACACAGTTTAGAAT1010.10939615488762525No Hit
CAGTAGGGGATTGTGGATTGTGAAAATGGCCCTCTGGAGCAATTGTGCTTTC1010.10939615488762525No Hit
CTTAGAGGGAGATATTTGTGAACTAGTCAGTATTTTAAAAAGCATACATTCT1010.10939615488762525No Hit
TAATTAGGGCGATCTAAACTGGGGCATAACCACACCAATAACGAAATAGAAG1010.10939615488762525No Hit
AAGTTTGGGCACAAATTGTGGTGTTGTTAGGTGACATTTAATATTTCTTGAG1010.10939615488762525No Hit
TGCTAGGGGGATCTACTTATTTACTTCCTTTCTCTTCTCAAAGATAATACAT1000.1083130246412131No Hit
ATTAGCGGGAGAAAGAGCATTTGAAGAGCAAAAGGCAAAGGTTCCATCACCA1000.1083130246412131No Hit
ATTAGTGGGGAAGTTTGAGGAGGTAGGTATGGAGGCACCTTAACACCAGTTT1000.1083130246412131No Hit
CGTACAAGGGAGAGTTTCGTAGGATGGAATATCAAAGAAGAGAGACCTGTAT1000.1083130246412131No Hit
TATTGAGGGATTGTCAGAAATTTTACAAGCCAAAACACAATAATGATGTCTT990.10722989439480098No Hit
ACCAGTGGGCTAGTCAGTGCCTTATCTCCTTCTGTCAAAGTTCGAGTCACTG990.10722989439480098No Hit
CACTGTGGGATGCTCAGCCTCTTTGACATAATTTTTGCAAATCAATGAATTG990.10722989439480098No Hit
CATACAGGGACTGTCTTCCCTTGGTCAGTGTTGTCTGTGTCCTCGTCTCCTC990.10722989439480098No Hit
GTATATGGGATTTCATTTAACACAGGAACTGAGACTTTTGAAGGAAGAAATC990.10722989439480098No Hit
GAGCAGGGGACGATGAAATATACCATAATTATTTCATTATAGCCTCTGCAAA990.10722989439480098No Hit
TTTTAAGGGCGGCATTCAGGTGTCTTGTAATTCCATAAAATTTTGGAGTTAG980.10614676414838886No Hit
ACCAGGGGGGAAAATATACATCTAGAATTGAAGAAATATAGTAATCAATGTA980.10614676414838886No Hit
ATTTGGGGGATCCAATTTAAGGTCATCTGAAAAATACAGGAAGGCAACGATG980.10614676414838886No Hit
TTACACCGGGGGGACAAAAGTATAAATATTATTTATTTCAGGTAGAATTTTT980.10614676414838886No Hit
TTCATTGGGAAGGGAGAGTGGTATTTGCAGACTAGAATTCTAGTGCTGCTGA980.10614676414838886No Hit
CATTTGGGGAAGCAACTGGATGATAGAGAAAGAAGCAGAAGTGGAAGAGGAA970.1050636339019767No Hit
CTGCATGGGACATCTCAATCATTTGTTCACATCGAATGAGAGAAAATAAAAA970.1050636339019767No Hit
GGTAATGGGATTCTTGGGAGTTCTAGGGCCACACTCACCACCTGTTCCTCCC970.1050636339019767No Hit
GGAAATGGGTGAAGTTGGACACTACTTGCTTGTCCCCACCCAGGCAGTCTTT970.1050636339019767No Hit
ATATTTGGGCTACATCAAAAAACAAGAAACATTTCAAATAAATAACAATGCA970.1050636339019767No Hit
ATTTTGGGGCCTATTGCCCAAGTATTGTCAAACTATGGTATTATTTTAATGT970.1050636339019767No Hit
GCATAGGGGGACGATCACCTTCTCCTTATTATAGTCGATATAGATCACGATC960.10398050365556459No Hit
TTCCGAGGGCACAGACTTTCCAACTCTCCAGTTTTACTGAATCAAATTTGTC960.10398050365556459No Hit
TAGCAAGGGCACTTTGGGGAGTACAATGCTTATTCTCAAAATGCTCACAACA960.10398050365556459No Hit
AGCGGCTGGGTGTGCCACCAATGAGAGCATTGGGTTCAAGTTTGCATCAAAC960.10398050365556459No Hit
ATCGGCGGGAAGACAGATTTTGTCAAACAAGCTGGTATCATGAGGAAGTCAG950.10289737340915245No Hit
TCCGATGGGATATACTTGGATCTACATAAAGAAAGATGGAGCCCAAGAAAAG950.10289737340915245No Hit
TAGGCTGGGGATCCGCAGGGCGCGGCAAGATGGAGGTGACGGGGGTGTCGGC950.10289737340915245No Hit
TTCACCGGGAATGCAAAGTTGGGAAGAACTGAAAAATGTTTCCAATTCATGT950.10289737340915245No Hit
CGATGCGGGAAGGAACTTAGCCTTGGATTCCTGAATAGAAAGGTAAGAATTC950.10289737340915245No Hit
ACAGCGAGGGAGATCAAAAATGGCGGTAGTTGGTGTGTCCTCGGTTTCTCGG950.10289737340915245No Hit
GAATTAGGGCCTTGCCTTATGTCCCACATTCCCCAGGGGATTGTCAGCTCCC950.10289737340915245No Hit
ACTTAAGGGAAGAAATAAAAGCATTATTGGGTGCCTTTGTTTGTAAACCAAA950.10289737340915245No Hit
CTCTGCAGGGATAATGGGAGTGAACATTCTCTGTGAATTATCAGAGTCCCTG950.10289737340915245No Hit
TCACTCGGGAACTGCATGTGTGTCTGTAGGTCTCAGTTTCCACATTCATACA940.10181424316274032No Hit
ACACATGGGATCTTCACCTCAAACCCTGTGAGGCAGGCATTGTTATCCTCTC940.10181424316274032No Hit
TAGCCGGGGCATACAGGGCATCACTTCTATTCAACATAGTACTTGGAAGTCC940.10181424316274032No Hit
TTTAATGGGAGCCTGGCTTGGGACTAAATGCCCTGCTCTGAACACGAAGCTT940.10181424316274032No Hit
TCACCTGGGCTTTCGGTGATTTGAGGTATGAAGTCTGTATCCAACAACATTC940.10181424316274032No Hit
CTGTTTGGGATGTTCATGGGCGTTTACACAGTGGAGTTACTGTTCATCATGG930.10073111291632819No Hit
AGTTCTGGGTGAAACTACTGCCACCTGCTCACTGCGAAACCCTCCGGTACCT930.10073111291632819No Hit
TGACTGGGGACCTCGCTTTAAACGACTCGCGGACATGTATGGGACTGGCCAA930.10073111291632819No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)