FastQCFastQC Report
Sat 31 Dec 2022
SRR5844784.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844784.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26610
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACACTTTGCGATCTCGTATGC4651.7474633596392335No Hit
CTTATACACATCTCCGAGCCCACGAGACACTTTGCGATCTCGTATGCCGTCT4391.6497557309282225No Hit
GAGCCCGGGACTGGGCAACTCAGAAATACTTCGATCTCCCAAGATATAAGAG1810.680195415257422No Hit
TTTTGTGGGATGGGGATCCAAGACCTGAGATAAAGCAACAGCCTGCCCAGAT1670.6275836151822622No Hit
GCAAATGGGGTAAAAAGCCCTTAAAAATATGTGTATACTACTTTGGCTCTTG1670.6275836151822622No Hit
CCTCAAGGGCGCTTTATCCTCATCCAGAACCGGGCAGGCAAGACGCGCCTGG1620.608793686583991No Hit
GTACCTGGGAGCGCTGGCCGCGTGCCGCCTCCGGAGCCGGCAGCCCCCATGG1580.5937617437053739No Hit
ACGGTAGGGCCTACCAGTGCAGTGAATGTGGCAAATGTTTCAGTCAGAGCTC1580.5937617437053739No Hit
ATCCAAGGGGTCATGAAATTGTTGTGACCTCTACTTTTTGTCACTAATAGCC950.3570086433671552No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTTTGCG950.3570086433671552No Hit
CCTCTCGGGTGGGTCTTCATTTTGAGATCGTTTTCTATGAGCTGAACTGAGG940.35325065764750097No Hit
CGTCACGGGCAGAGCTGAGCCCCGGCCTGGCTGCCCCTCCCCTCCGGCCCTC910.3419767004885381No Hit
AGAAGTGGGATGTGCAAAGAAGTCCTATGAATCTATATTTTAAATTTACTGA890.33446072904922963No Hit
TACTGCGGGGTAATGTTTTGCACTTTTTCGTACAGGGTGGGCGGGGGCGGGA820.30815482901164976No Hit
GTCTCAGGGAGCCTTTTCAGATAATTACACATAATCTTCTTTAATACCACAC750.2818489289740699No Hit
TCTATTGGGACACCAAATGGGTGATATTTTGAAAAAAGTTTGTGTTTTACTG730.2743329575347614No Hit
CAACTGGGATGCCCTCCTATGAGAATCTAATGCCGCTGCTAATCTGACAGGA690.2593010146561443No Hit
ATATGCGGGAACAGACTGAATATATTTTACCATTACAGGGCTAAAAGGAGTC680.25554302893649006No Hit
TAATGTGGGGGTGGCTGTGTCGTGCGCGTGAGCCCCGTAGGGCCGGGGAGGC630.23675310033821872No Hit
GTTATCGGGAAACCAGGCCCGCTTCACAAGTTAGAGTTGAGCTCCCCTGTAG610.22923712889891018No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACACTTTGCGA460.1728673431040962No Hit
GAGACAGGGTGATTGGCCAGCCGAGCGAGAACCAGGCTGCTGCATGGCACTG390.14656144306651636No Hit
CTAGAGGGAAAATAATCGATGCAAGGAATGGATTTAGGGAAAATGCTTCTTT390.14656144306651636No Hit
GGCTCTGGGACTTTGCAACTTTAATAAGATCCACTTAGCTGGAAACTCATTT350.13152950018789927No Hit
TCACAAGGGAGTGGTTGAGTGATAGAGTTGTTGAATAAAGGATAAAGTAGCA330.12401352874859076No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTTTGCGAT320.12025554302893648No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACTTTGCGATCCTGTCTCT310.11649755730928223No Hit
CTTATACACATCTCCGAGCCCACGAGACACTTTGCGATCCTGTCTCTTATAC290.1089815858699737No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)