FastQCFastQC Report
Sat 31 Dec 2022
SRR5844783.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844783.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27031
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACACTTTGCGATCTCGTATGC4451.6462580000739893No Hit
CTTATACACATCTCCGAGCCCACGAGACACTTTGCGATCTCGTATGCCGTCT4311.5944656135548074No Hit
CCTCAAGGGCGCTTTATCCTCATCCAGAACCGGGCAGGCAAGACGCGCCTGG2030.7509896045281345No Hit
GAGCCCGGGACTGGGCAACTCAGAAATACTTCGATCTCCCAAGATATAAGAG1850.6843993932891865No Hit
GCAAATGGGGTAAAAAGCCCTTAAAAATATGTGTATACTACTTTGGCTCTTG1840.6806999371092449No Hit
GTACCTGGGAGCGCTGGCCGCGTGCCGCCTCCGGAGCCGGCAGCCCCCATGG1710.6326070067700048No Hit
ACGGTAGGGCCTACCAGTGCAGTGAATGTGGCAAATGTTTCAGTCAGAGCTC1570.5808146202508232No Hit
TTTTGTGGGATGGGGATCCAAGACCTGAGATAAAGCAACAGCCTGCCCAGAT1520.5623173393511154No Hit
ATCCAAGGGGTCATGAAATTGTTGTGACCTCTACTTTTTGTCACTAATAGCC1260.46613147867263516No Hit
TCTATTGGGACACCAAATGGGTGATATTTTGAAAAAAGTTTGTGTTTTACTG1050.3884428988938626No Hit
CCTCTCGGGTGGGTCTTCATTTTGAGATCGTTTTCTATGAGCTGAACTGAGG1000.3699456179941549No Hit
TACTGCGGGGTAATGTTTTGCACTTTTTCGTACAGGGTGGGCGGGGGCGGGA960.35514779327438867No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTTTGCG900.3329510561947394No Hit
CGTCACGGGCAGAGCTGAGCCCCGGCCTGGCTGCCCCTCCCCTCCGGCCCTC730.27006030113573304No Hit
AGAAGTGGGATGTGCAAAGAAGTCCTATGAATCTATATTTTAAATTTACTGA720.2663608449557915No Hit
TAATGTGGGGGTGGCTGTGTCGTGCGCGTGAGCCCCGTAGGGCCGGGGAGGC690.25526247641596683No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACACTTTGCGA670.24786356405608378No Hit
CAACTGGGATGCCCTCCTATGAGAATCTAATGCCGCTGCTAATCTGACAGGA640.2367651955162591No Hit
GTCTCAGGGAGCCTTTTCAGATAATTACACATAATCTTCTTTAATACCACAC630.23306573933631758No Hit
GTTATCGGGAAACCAGGCCCGCTTCACAAGTTAGAGTTGAGCTCCCCTGTAG590.21826791461655137No Hit
CTAGAGGGAAAATAATCGATGCAAGGAATGGATTTAGGGAAAATGCTTCTTT530.19607117753690206No Hit
GAGACAGGGTGATTGGCCAGCCGAGCGAGAACCAGGCTGCTGCATGGCACTG520.19237172135696054No Hit
ATATGCGGGAACAGACTGAATATATTTTACCATTACAGGGCTAAAAGGAGTC450.1664755280973697No Hit
CTTATACACATCTCCGAGCCCACGAGACACTTTGCGATCCTGTCTCTTATAC440.16277607191742813No Hit
GGCTCTGGGACTTTGCAACTTTAATAAGATCCACTTAGCTGGAAACTCATTT340.12578151011801267No Hit
CGACTCGGGGGGAAGAAATAAGGGTTGAGGTGTGATAAAGATGTGCTAGGCC310.11468314157818801No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTTTGCGAT310.11468314157818801No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACTTTGCGAACTCGTATGC290.10728422921830491No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACTTTGCGATCCTGTCTCT290.10728422921830491No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)