FastQCFastQC Report
Sat 31 Dec 2022
SRR5844782.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844782.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26384
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACACTTTGCGATCTCGTATGC4281.6221952698605218No Hit
CTTATACACATCTCCGAGCCCACGAGACACTTTGCGATCTCGTATGCCGTCT3621.3720436628259551No Hit
GAGCCCGGGACTGGGCAACTCAGAAATACTTCGATCTCCCAAGATATAAGAG1890.7163432383262583No Hit
CCTCAAGGGCGCTTTATCCTCATCCAGAACCGGGCAGGCAAGACGCGCCTGG1890.7163432383262583No Hit
GCAAATGGGGTAAAAAGCCCTTAAAAATATGTGTATACTACTTTGGCTCTTG1620.6140084899939358No Hit
GTACCTGGGAGCGCTGGCCGCGTGCCGCCTCCGGAGCCGGCAGCCCCCATGG1540.583687083080655No Hit
ACGGTAGGGCCTACCAGTGCAGTGAATGTGGCAAATGTTTCAGTCAGAGCTC1510.5723165554881746No Hit
TCTATTGGGACACCAAATGGGTGATATTTTGAAAAAAGTTTGTGTTTTACTG1310.49651303820497267No Hit
TTTTGTGGGATGGGGATCCAAGACCTGAGATAAAGCAACAGCCTGCCCAGAT1180.4472407519708914No Hit
CCTCTCGGGTGGGTCTTCATTTTGAGATCGTTTTCTATGAGCTGAACTGAGG980.37143723468768947No Hit
CGTCACGGGCAGAGCTGAGCCCCGGCCTGGCTGCCCCTCCCCTCCGGCCCTC960.3638568829593693No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTTTGCG940.35627653123104913No Hit
AGAAGTGGGATGTGCAAAGAAGTCCTATGAATCTATATTTTAAATTTACTGA880.3335354760460885No Hit
ATCCAAGGGGTCATGAAATTGTTGTGACCTCTACTTTTTGTCACTAATAGCC860.32595512431776835No Hit
CAACTGGGATGCCCTCCTATGAGAATCTAATGCCGCTGCTAATCTGACAGGA810.3070042449969679No Hit
GTTATCGGGAAACCAGGCCCGCTTCACAAGTTAGAGTTGAGCTCCCCTGTAG780.2956337174044876No Hit
TACTGCGGGGTAATGTTTTGCACTTTTTCGTACAGGGTGGGCGGGGGCGGGA760.2880533656761674No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACACTTTGCGA750.28426318981200727No Hit
GTCTCAGGGAGCCTTTTCAGATAATTACACATAATCTTCTTTAATACCACAC710.2691024863553669No Hit
TAATGTGGGGGTGGCTGTGTCGTGCGCGTGAGCCCCGTAGGGCCGGGGAGGC590.2236203759854457No Hit
GAGACAGGGTGATTGGCCAGCCGAGCGAGAACCAGGCTGCTGCATGGCACTG510.19329896907216496No Hit
ATATGCGGGAACAGACTGAATATATTTTACCATTACAGGGCTAAAAGGAGTC430.16297756215888418No Hit
GGCTCTGGGACTTTGCAACTTTAATAAGATCCACTTAGCTGGAAACTCATTT410.15539721043056398No Hit
CTAGAGGGAAAATAATCGATGCAAGGAATGGATTTAGGGAAAATGCTTCTTT390.1478168587022438No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTTTGCGAT360.13644633110976348No Hit
CTTATACACATCTCCGAGCCCACGAGACACTTTGCGATCCTGTCTCTTATAC340.12886597938144329No Hit
AGTGCAGGGCAAGACAGGCTAGTTCATAAACAAACTGAATGTGTAACTTCTC320.1212856276531231No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACTTTGCGAACTCGTATGC310.117495451788963No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACTTTGCGATCCTGTCTCT300.11370527592480292No Hit
TCACAAGGGAGTGGTTGAGTGATAGAGTTGTTGAATAAAGGATAAAGTAGCA270.10233474833232263No Hit
CGACTCGGGGGGAAGAAATAAGGGTTGAGGTGTGATAAAGATGTGCTAGGCC270.10233474833232263No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)