FastQCFastQC Report
Sat 31 Dec 2022
SRR5844772.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844772.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184386
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCAGGACTCATCTCGTATGC9210.4994956233119651No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGGACTCATCTCGTATGCCGTCT8330.4517696571323202No Hit
TCGTACGGGAGGCCATGGGCTGGCGGGAAGGCTCTGGCTTGGGACGAAAGTG2880.15619407113338324No Hit
TTACTTGGGGAAGTCAGGATGCCATAAAAAGGAGCAAAAGAGAATGAAAAAA2790.15131300641046502No Hit
ACGACCGGGATCATTTCCATCTTTAGTATGTTACATTTCATTAGAACTTGAG2600.14100853643985986No Hit
CGCTCAGGGATGGAGGTGATTTGTCCAGGTTTCTTTTTTTTTTTTTTTTTTT2530.13721215276647902No Hit
ACGACAGGGAGCCCAGTATTGCCTTAAATAAAATCACATTTCATTTCTTGTT2490.13504279066740424No Hit
TGTTTAGGGGTGGCCTGGTGCCTCTCCCAGCCAGCACAGGACCTGGATGAAT2350.12745002332064256No Hit
CTGTCTGGGCACTGGATGGGAGGGCAATGAGTGTGCACATACAAATGGGGCA2320.12582300174633648No Hit
GAACAACGGGGTCACCTGCGTGGTCCATTCCATAAACACCAGATTCCATTTC2300.1247383206967991No Hit
CGCGCAGGGGGAAGGTGGTTTATTACAAATAAGCACACTAACCCCTCGGAAG2270.12311129912249304No Hit
ACAACTGGGCCATGAAAACTGCACCTAATTTACCACACTTAAGATTGGCAAT2260.12256895859772433No Hit
TATGTGGGGGGGTAAATAACAAAATTAAGGGAGAAATAAATAAGTTCTTTGA2230.12094193702341827No Hit
TTTTTAGGGGTCTCTAATTTCATTGATTTTCGAGGGCACATGCTTTAGTTGA2220.12039959649864956No Hit
GACGAGGGTATGCATTGTTAGCTGACAAGAAAGATCAAGCACAATTAGATGT2210.11985725597388089No Hit
CGCTACGGGTATCAATACCTGGCAAACTGACCAGAATTACCTTCCTCATGGC2210.11985725597388089No Hit
TAGCGTGGGGTCCAATTGTATACCTAGAATACTTACTTAAAACTTAGTTTAC2190.1187725749243435No Hit
CATCAGGGGTGACTTCCTGCCATGATAGGTGAGGATTTAGCAAACTCACTCC2170.11768789387480612No Hit
ACAGACGGGACTATAAACTCTTTTCTCATAAGACTTGCCAAAATTTAATTTT2150.11660321282526874No Hit
TAGTCGGGGATCCTTGTCGGGGGCCTTGGTTTGGCAATGCTGGTGGCTTTGA2150.11660321282526874No Hit
CTTTATGGGATTTTAAAAAAGTGATTTTTCTTGAGAGCTTTGTTATTCTTAA2130.11551853177573135No Hit
AAGATGGGGAATAACAACTGAGCCAGACCTGCACACGCCGAGGGCCCCGGGA2130.11551853177573135No Hit
CAAGCAGGAAGTGAAATTGTCCCTGTTTGCAGATGACATGATTGTATATATA2130.11551853177573135No Hit
TGCCTAGGGCTGAGATTTAAGTCTCTCTGGCCCTAAGAGGTTTTTCCTCTTT2120.11497619125096264No Hit
TCTAGGGGATCAGTGGAAATCCAGCGAGATATTAGAAATAAAGGCCAGAAAA2120.11497619125096264No Hit
CTAGAGTAGGGGTGTTATGTGAAGTCACATAATGTAGGTTAGTTTACAGTAA2120.11497619125096264No Hit
TTGATGGGGAGCTCCTGAGATCCTGCAGGAGTTGGAGTATGGCCCCTCCGTG2090.11334916967665658No Hit
TTTGGCGGGGTGGTATGTTAACTTCTAACATTTTAAAAAATTTCTTCAGAGG2080.11280682915188787No Hit
GTACAGGGGCCTTTTCCCACCCCCTAGCGCCGCTGGGCCTGCAGGTCTCTGT2070.1122644886271192No Hit
TGGCACGGGGTGCTGGGACCCGAGTAGGAGCTAGGACTAGCCAGGACCCGCA2070.1122644886271192No Hit
TTTTTAGGGGGTGATGATGGGCAGCCTGGTGTACCTGCGGCTGGGCTTGGAG2060.1117221481023505No Hit
TGCGCTGGGCTAGGAACTGTGTTGGAACTCTTTAAAATAAAGAAAAGAGGAG2060.1117221481023505No Hit
CACGTGGGGGTGAATAACCTTGAATCCTTTCAAAGAGTGGAGCTTCTGAAGA2060.1117221481023505No Hit
TGTAGAGGGGAAATCCCTATTACATACAATTGGAAAATAAACAGTGTTGGAG2050.11117980757758181No Hit
ATTTAGGGATACCCGGACCGGGTGCCGGTGATAGTAGAAAAGGCTCCCAAAG2040.1106374670528131No Hit
AGTTATGGGGAGGGCATTTTAGCTTCCTTTCTGAGTAATCCTTGCTTTAGCA2040.1106374670528131No Hit
TGGCCTGGGATGAAGGAGGGATATTAAATGGGCCCTTATTTGATCATTTTGC2040.1106374670528131No Hit
TAATTTGGGGGGGGAGAAGGGACGAGGAGGGGCGGACAGCGGAAGGTCCGGG2040.1106374670528131No Hit
CAGATAGGGTTTCCAAGTCAGGAGCTTGAAAGGGGTGAAAAGGAAAGGCCAC2040.1106374670528131No Hit
TGGCACGGGATGTAAAATGAGTGAAATCTGGGTGTTGGTAGATTGTATCAAT2030.11009512652804443No Hit
AATCATGGGGAAAAAATAAATTTTTAAATGACTAGATGAGAAACTTGAATAT2020.10955278600327575No Hit
GCTGGGGGTAGGAAAGGAAAACACAAAGATCGATAGAATTTTATTCTCTGGT2020.10955278600327575No Hit
TCTGAGAGGGAGGCGTGGGGCTCTCTCCTTGTCAGTCGGCGCCGCGTGCGGG2020.10955278600327575No Hit
GGGCATGGGGCACCTTTCTGGAATTATTTTGCCAAAATATTTAACCTGAATC2020.10955278600327575No Hit
GCATTGGGATCACTTTAAATTTTATGTTTTTCTAAGGAAACTTTCCACAGAA2010.10901044547850705No Hit
TCTAATGGGTCGATGTTTTCAGACCATTGATGTTAAAGTGCTTATTGACAGT2010.10901044547850705No Hit
GTGAACGGGTAAAACATACACTAATCTACTATAAAATAAAAATTTGTCAAAA2000.10846810495373836No Hit
TTCTGTGGGTGCTGGTAATTTCTTTGTGTCAAAATTTTGAGACTAGATCACT2000.10846810495373836No Hit
GCATAGCGGGCTGGATCTGTGGGCCACAAGCATATCGACAACTGCCAGCTAA2000.10846810495373836No Hit
TAAGCCGGGGTGGAGTTTTCTGTTATATTTTGTAATTGGTTATTAGTTGTGT1990.10792576442896966No Hit
CCTAATGGGACGCTCTTCCGATCTGCAACTGGGAGACAGTCCCACCCTCACA1990.10792576442896966No Hit
GCTTTGGGGGACTTTTCAGAAATTAGAAAATTGTTGTGATAGATAAGGGTAA1980.10738342390420098No Hit
AGGTTGGGTACCAGTGTAACTAAGCATTTGTGTTCTGATATCTGAGGCCAGT1980.10738342390420098No Hit
TATTATGGGAAGGAACCTTAGAACATAATCTTACCTGGCTCAGTACAAGTAG1970.10684108337943228No Hit
ATTGTGGGGAGGTGAGTGGAGAAGACGTCCTGGTTTCTCCAAATAACTAAGA1970.10684108337943228No Hit
TTAGGTGGGATAGAGACCAAAGTAAATAGGATTATTATTGCATTGTGTAATT1970.10684108337943228No Hit
AAACAAGGGATACAAAGATACTACACAAAAATAGAACAATTAGTCTGAATTA1970.10684108337943228No Hit
TTACCTGGGGTCCGGTGGGCTAGGTACTGAGCGCGCGAGGTGAGGAGTTGTG1960.10629874285466359No Hit
CATGGGGGGAGAATAAACTGACAGAAAAGCTTTTGTTGAAGTATGATTGATT1960.10629874285466359No Hit
ATGCATGGGCAAAAGAAACAAGGAATTCTAATATACCAGCAGAAGGTAGAAA1960.10629874285466359No Hit
AGCACTGAAATTAAAATACACCATTTGCAATCCCTAAACAAACAAACAAAAC1960.10629874285466359No Hit
TATTATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1950.10575640232989489No Hit
ATTCTTTCATTTTTTATACCTGAATTGTTACTGTGACTTAACTGCTGCATAC1950.10575640232989489No Hit
TCCACAGGGGGAGCACCTCTGCCCGGCCACCCCGTCTGGGAAGTGAGGAGCG1950.10575640232989489No Hit
GCCAGAGGGGGGCTCCACAATGTTAAGCAATATTTTAGAAAAGGGTACAATT1950.10575640232989489No Hit
GAACCAGGGCAGTAAACCTGTGATAGATAATTTATTTAACTGGAAAACCTAG1940.10521406180512621No Hit
CACCGGGGCGGGGTGGCAGAAGCAGTACACTTGGTGTAACTGTATGGAAATA1940.10521406180512621No Hit
AACAACGGGGTGACTTTTCTTGTGGGAATGTGTTGTATTTTCTTGGTTCTTC1940.10521406180512621No Hit
TAGCAAGGGATAGATTAAGCTTTCAGGGATTTGGAAATCTTTTTTTCTTTCT1940.10521406180512621No Hit
TTTACCGGGGTAGCTATAAAGTGACAAGCTGTTTTTAATTTATCACATACAC1930.10467172128035751No Hit
GCCAGAGGGATCTGATTACATGATCTCTGATTTAAACAAACAAAACACTTAT1930.10467172128035751No Hit
TTGACAGGAACCAAAATAAACAAAGCAAGACAAATTAAAAATATGTGGGGGA1930.10467172128035751No Hit
ACATTTGGGGTTGGCCTGAACTTTAGGAAATGAGGTCGTAAGTCGAAAGAAC1930.10467172128035751No Hit
GAATAGGGGGCCAGGTGATTAAGTACGTGCTCCAGGATCCCATTTGGCTGCT1930.10467172128035751No Hit
GCTAATGGGACAGAGTCTTTTGAAACAAAATTGGTTTCAAGTGAAACTTACA1930.10467172128035751No Hit
TATGTCGGGAGGTCAGATTGTCAAGCATGGAGATATCAAGTGTGTACTAAAT1920.10412938075558882No Hit
GCATGAGGGGATAGATATGCTAACTAGCGTAACTGAGGTAATTTAAAGACTA1920.10412938075558882No Hit
CTTTAAGGGTAAAAAAATGAAAGTCAAATGGTGTCAGTTACAATTGCAAAAT1910.10358704023082012No Hit
ACGCAGGGTGTTCATAAAGAGTTTCAACAGCAGACTCAATCAAGCAGATTTA1910.10358704023082012No Hit
AGTTCAGGGACCTGGAAGGCACCTTGCTGAAGCCCAACATGGTCACCCCAGG1900.10304469970605144No Hit
CTAAGAGGGGAGACGGCTGGGCGCCGAGTGGGACAGCGCTGGTGCGGAGACT1900.10304469970605144No Hit
TATGCAGGGAACTGATGCATCCATACTCAGGGTGTAGGGAGAATACTTTGCA1900.10304469970605144No Hit
CAAAAAGGGATTTAGGAAAGACTAAGCAACCAAATAGCAAAGATCCCTCAGG1900.10304469970605144No Hit
ATAAAAGGGAATACAAACTGGATTATGTATTTTTTTATGATGTTGATTGGTT1890.10250235918128274No Hit
TTAGAAGGGTTACAGGTACAAACTCACTAACACTTGATGTTATGCAGAAATA1890.10250235918128274No Hit
AGAAATGGGGTTACTTGACTATTAGAAAATTCATTGTATATTTAAAAAGCGA1880.10196001865651405No Hit
GTTTTGGGGTCCTACCTTGAGATGTAGCCACAGAAAAATGATCTCTCTCTCT1870.10141767813174535No Hit
ATCGGGCATTACCACCCATGTTATTATTTATTATTTTTCAGAAATCTGGTCT1870.10141767813174535No Hit
TCAGAGGGGAGCCTAAGTGCAATTGATGTATGGTAATGCTACTGGTTTTTAT1870.10141767813174535No Hit
ATATGGGGGATCATAACAAATGGTTACAAAATGGCAGCATTGGACCAAGGAC1870.10141767813174535No Hit
CGCATAGGGGGGCGGCGGTGGCGGCTGTGGCAGCGGGAGCAGCTGCGCCGCC1870.10141767813174535No Hit
GCAATCGGGTCATATGAAGGTGGGAGTGATTTCTAGATTGGGCCCCTACTGG1860.10087533760697667No Hit
ACCTCCGGGTATTAAAATGCATTTAATTATAATAAAACATTTAACACAAGTA1860.10087533760697667No Hit
GCTATTGGGCAGGTTTGGCTGTTTGGCTGTCTGGTAAGAGCTGCATTCTCCA1850.10033299708220797No Hit
GAAAGTGGGAAGACATCATATTTTGCCCCATAAATATGTAATTATTTGTCAA1850.10033299708220797No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)