FastQCFastQC Report
Sat 31 Dec 2022
SRR5844770.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844770.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184619
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCAGGACTCATCTCGTATGC8510.46094930640941617No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGGACTCATCTCGTATGCCGTCT7890.4273666307368147No Hit
TCGTACGGGAGGCCATGGGCTGGCGGGAAGGCTCTGGCTTGGGACGAAAGTG2720.1473304481120578No Hit
GAACCAGGGCAGTAAACCTGTGATAGATAATTTATTTAACTGGAAAACCTAG2500.13541401480887666No Hit
ACAGACGGGACTATAAACTCTTTTCTCATAAGACTTGCCAAAATTTAATTTT2380.12891414209805058No Hit
CGCTACGGGTATCAATACCTGGCAAACTGACCAGAATTACCTTCCTCATGGC2370.12837248603881507No Hit
TGTTTAGGGGTGGCCTGGTGCCTCTCCCAGCCAGCACAGGACCTGGATGAAT2340.12674751786110855No Hit
TTACTTGGGGAAGTCAGGATGCCATAAAAAGGAGCAAAAGAGAATGAAAAAA2320.12566420574263754No Hit
CTAAATGGGCCATAGTATTTTTAGGCATTCCTCTAAATTTTTCAGAGTCAAG2240.12133095726875348No Hit
ACAACTGGGCCATGAAAACTGCACCTAATTTACCACACTTAAGATTGGCAAT2240.12133095726875348No Hit
ACGACCGGGATCATTTCCATCTTTAGTATGTTACATTTCATTAGAACTTGAG2230.12078930120951797No Hit
TATGTGGGGGGGTAAATAACAAAATTAAGGGAGAAATAAATAAGTTCTTTGA2200.11916433303181147No Hit
TAGTCGGGGATCCTTGTCGGGGGCCTTGGTTTGGCAATGCTGGTGGCTTTGA2180.11808102091334044No Hit
AAGGCTGGGAAGACTTGTCAGACCATCAATCTCTTGGGGTGATTAATCAAAT2170.11753936485410495No Hit
GCATTGGGATCACTTTAAATTTTATGTTTTTCTAAGGAAACTTTCCACAGAA2170.11753936485410495No Hit
TTTTTAGGGGTCTCTAATTTCATTGATTTTCGAGGGCACATGCTTTAGTTGA2160.11699770879486943No Hit
TTCCCTGGGACCCAGGCTGGAGTGTAGCGTGGGGTCACGGCTCACTGCAGCT2140.11591439667639843No Hit
ATGCATGGGCAAAAGAAACAAGGAATTCTAATATACCAGCAGAAGGTAGAAA2120.11483108455792741No Hit
CTAGAGTAGGGGTGTTATGTGAAGTCACATAATGTAGGTTAGTTTACAGTAA2110.1142894284986919No Hit
CGCGCAGGGGGAAGGTGGTTTATTACAAATAAGCACACTAACCCCTCGGAAG2110.1142894284986919No Hit
GAATAGGGGGCCAGGTGATTAAGTACGTGCTCCAGGATCCCATTTGGCTGCT2110.1142894284986919No Hit
CTGTCTGGGCACTGGATGGGAGGGCAATGAGTGTGCACATACAAATGGGGCA2110.1142894284986919No Hit
GCTTTGGGGGACTTTTCAGAAATTAGAAAATTGTTGTGATAGATAAGGGTAA2100.11374777243945638No Hit
GACGAGGGTATGCATTGTTAGCTGACAAGAAAGATCAAGCACAATTAGATGT2100.11374777243945638No Hit
TAGCGTGGGGTCCAATTGTATACCTAGAATACTTACTTAAAACTTAGTTTAC2100.11374777243945638No Hit
CATCAGGGGTGACTTCCTGCCATGATAGGTGAGGATTTAGCAAACTCACTCC2080.11266446032098539No Hit
CAATATAGGGCTAAGAATTCAGAAGAGTAACCCTTTGTCAGAAACATCAAAG2080.11266446032098539No Hit
GCTAATGGGACAGAGTCTTTTGAAACAAAATTGGTTTCAAGTGAAACTTACA2080.11266446032098539No Hit
GAACAACGGGGTCACCTGCGTGGTCCATTCCATAAACACCAGATTCCATTTC2070.11212280426174986No Hit
TAGCAAGGGATAGATTAAGCTTTCAGGGATTTGGAAATCTTTTTTTCTTTCT2070.11212280426174986No Hit
TTCTGTGGGTGCTGGTAATTTCTTTGTGTCAAAATTTTGAGACTAGATCACT2060.11158114820251437No Hit
CAGATAGGGTTTCCAAGTCAGGAGCTTGAAAGGGGTGAAAAGGAAAGGCCAC2060.11158114820251437No Hit
TTTACCGGGGTAGCTATAAAGTGACAAGCTGTTTTTAATTTATCACATACAC2050.11103949214327886No Hit
TGGCCTGGGATGAAGGAGGGATATTAAATGGGCCCTTATTTGATCATTTTGC2050.11103949214327886No Hit
AACAACGGGGTGACTTTTCTTGTGGGAATGTGTTGTATTTTCTTGGTTCTTC2050.11103949214327886No Hit
GTTTTGGGGTCCTACCTTGAGATGTAGCCACAGAAAAATGATCTCTCTCTCT2040.11049783608404334No Hit
AGCGCAGGGATGAAAGAAGAATAATAATGTGACATACCAATTGTTTATTATG2030.10995618002480785No Hit
AGAAATGGGGTTACTTGACTATTAGAAAATTCATTGTATATTTAAAAAGCGA2030.10995618002480785No Hit
AGCACTGAAATTAAAATACACCATTTGCAATCCCTAAACAAACAAACAAAAC2020.10941452396557233No Hit
ACGACAGGGAGCCCAGTATTGCCTTAAATAAAATCACATTTCATTTCTTGTT2010.10887286790633684No Hit
AACATAGGGGCTTATACACTATTGGTGGGAATGTGAATTAGCACGATCTCTA2010.10887286790633684No Hit
GCAGCGGGGTGAGACCCTGTCTCTTAAAAAGATAAAAAATGAGAAGATTAGC1990.10778955578786581No Hit
ACATTTGGGGTTGGCCTGAACTTTAGGAAATGAGGTCGTAAGTCGAAAGAAC1990.10778955578786581No Hit
CAAAATGGGTTCCCAATGGTCAAAGCTGGGATAATTTGAGCAACAAAATAAA1980.10724789972863032No Hit
TGCGCTGGGCTAGGAACTGTGTTGGAACTCTTTAAAATAAAGAAAAGAGGAG1970.10670624366939481No Hit
CACGTGGGGGTGAATAACCTTGAATCCTTTCAAAGAGTGGAGCTTCTGAAGA1970.10670624366939481No Hit
TATGTCGGGAGGTCAGATTGTCAAGCATGGAGATATCAAGTGTGTACTAAAT1970.10670624366939481No Hit
ATCGGGCATTACCACCCATGTTATTATTTATTATTTTTCAGAAATCTGGTCT1970.10670624366939481No Hit
TCGTGTGGGTCTTCAAAATGATATATGTATATAAATATCTGTATTTTGTTCA1960.10616458761015929No Hit
GAAAGTGGGAAGACATCATATTTTGCCCCATAAATATGTAATTATTTGTCAA1960.10616458761015929No Hit
AGCCTGGGCGACAGAGCGAGACTCCACCTCAAAAAATAAAATCAAGATAACC1950.1056229315509238No Hit
ACAGTGGGGAGTGTATATTGTTTGAGGTATAAACATGATTATAATGTACAAA1950.1056229315509238No Hit
GTACAGGGGCCTTTTCCCACCCCCTAGCGCCGCTGGGCCTGCAGGTCTCTGT1940.10508127549168829No Hit
TGTCCAGGGCATTAGACAAAATCCGGCATCTCTTTATGATTAAAACTCTCAG1940.10508127549168829No Hit
TTGGCTGGGGATACCTGTCACCCAGGGTATCTTAAAAGCAAGTTGTTCTCCC1940.10508127549168829No Hit
ACGCAGGGTGTTCATAAAGAGTTTCAACAGCAGACTCAATCAAGCAGATTTA1940.10508127549168829No Hit
ACAGTAGGGCGATCTTATACTGGGATTAACAGACAATGGGAAAACTAGCTCA1940.10508127549168829No Hit
CATGGGGGGAGAATAAACTGACAGAAAAGCTTTTGTTGAAGTATGATTGATT1940.10508127549168829No Hit
GCGCATGGGCCATCACACTGGGCATTAGGGCCTCAACATATGAATCAGGAGG1930.1045396194324528No Hit
TCTAGGGGATCAGTGGAAATCCAGCGAGATATTAGAAATAAAGGCCAGAAAA1930.1045396194324528No Hit
TACCAGGGTAATTTATGTTTAATACAAAAGGATGATTTAATGGTATCACCAA1930.1045396194324528No Hit
CAATTTGGGATATAGAGAGATCAATAGATAGATAATTAGAATATTATTTAGC1930.1045396194324528No Hit
GGGTGTGGGAATAAAACTTGGTTGGCTTGATATTTTAAGGAATTGAAACCTA1930.1045396194324528No Hit
TACAGTGGGACAAAGGTGCTGGGTAGACTAGTGGCAGCTCTCATGTGCTGCA1920.10399796337321728No Hit
TCAGGTGGGCAAGATATAAATAATAATAAAATTTGAAATTACATTAATTATT1910.10345630731398175No Hit
TTTGGCGGGGTGGTATGTTAACTTCTAACATTTTAAAAAATTTCTTCAGAGG1910.10345630731398175No Hit
CTTTAAGGGTAAAAAAATGAAAGTCAAATGGTGTCAGTTACAATTGCAAAAT1890.10237299519551075No Hit
GTCATTGGGAAGTTTTTTTCTCTTTGAAAGATAGAGATTAATACAACTACTT1890.10237299519551075No Hit
TCTAATGGGTCGATGTTTTCAGACCATTGATGTTAAAGTGCTTATTGACAGT1890.10237299519551075No Hit
GCATAGCGGGCTGGATCTGTGGGCCACAAGCATATCGACAACTGCCAGCTAA1890.10237299519551075No Hit
AATGATGGGCCTCCTTTAAGATGAGTTCCTTTCCCCAAAGTGACTTTCCTTC1890.10237299519551075No Hit
GTGAACGGGTAAAACATACACTAATCTACTATAAAATAAAAATTTGTCAAAA1880.10183133913627523No Hit
TGCTCAAGGGTAACAAACACTACAGAATAAGCAGCTTAAACAACAGAAATTT1880.10183133913627523No Hit
TAAGCCGGGGTGGAGTTTTCTGTTATATTTTGTAATTGGTTATTAGTTGTGT1880.10183133913627523No Hit
TTAGAAGGGTTACAGGTACAAACTCACTAACACTTGATGTTATGCAGAAATA1880.10183133913627523No Hit
AATTATGGGACAAGAAGACATACTGAAATTTCCTTAAGAGCAGGGCTCTCAT1880.10183133913627523No Hit
TATTATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1870.10128968307703974No Hit
TTGAAATGGGAATAATCACTATTCTCTTATTCTTTAGTTTTCTTATTGTGTT1870.10128968307703974No Hit
TGACCTGGGTATCCAGTGTTTGTCAGGGAGTGGGAAACCTATTTGCACACAG1870.10128968307703974No Hit
CATACTGGGTAGTAATGTCTTGCTATAATTTTGTATTTGTGGCACAAAATCT1870.10128968307703974No Hit
TACATTGGGGACTGACGCGCAGTGCCAGACCTTACCCCTCACGGTCCTTAAG1870.10128968307703974No Hit
TCTGGTGGGAGAGTTTAGAATTGATCATTTTCTCCCATGATAGCAAAGAAAA1870.10128968307703974No Hit
GCAATCGGGTCATATGAAGGTGGGAGTGATTTCTAGATTGGGCCCCTACTGG1860.10074802701780423No Hit
AACAACGGGACCACTGAAAATCGAAGCTGGGGAATTACATGTTCCTTTCACT1860.10074802701780423No Hit
GGTATAGGGCAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAAC1860.10074802701780423No Hit
GTAAGCGGGCACTTCCGCTTCCGCTGGGGAGGTCCTCCATGCGCAGTCATGA1860.10074802701780423No Hit
AATCATGGGGAAAAAATAAATTTTTAAATGACTAGATGAGAAACTTGAATAT1860.10074802701780423No Hit
TTACCTGGGGTCCGGTGGGCTAGGTACTGAGCGCGCGAGGTGAGGAGTTGTG1860.10074802701780423No Hit
GCTGATGGGGATATAGAATCAGAAGGCAAAAGCCTTAATAAAAATACAAATT1860.10074802701780423No Hit
CTTACAGGGTGTGAAAAGAGTATGTAGTGGCTTCTTTTGAACTGTTTGATAC1860.10074802701780423No Hit
CATACTGGGAAGTGAGAGATGGAAAGAAATAGGGACAACTCATCGAAATCAA1850.10020637095856874No Hit
TTTTTAGGGGGTGATGATGGGCAGCCTGGTGTACCTGCGGCTGGGCTTGGAG1850.10020637095856874No Hit
ACGAAGGGATTGCTCAGTAATGAGGCTGCAATTCTTCTCTGCGGAATCCAGG1850.10020637095856874No Hit
TGTACTGGGTCCAGCAGGTATTTTTCTGGAGTCTTTAGGGTTTTCTACATAT1850.10020637095856874No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)