FastQCFastQC Report
Sat 31 Dec 2022
SRR5844769.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844769.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29598
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCAGGACTCATCTCGTATGC4511.5237516048381647No Hit
TAAAGGAGGGAAAGAGAATTCAAAAAGGGCTGATGGTAGGCTTTGAACATGG3601.216298398540442No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGGACTCATCTCGTATGCCGTCT3221.087911345361173No Hit
GCGTATGGGGAAAATGCAAAGAGCAAACAAAAGAAAAACAAATAAAAAACAG3151.0642610987228867No Hit
CATCTGGGTCAAAGGAATTTTAACAAAGAGCAATCAGTATTATTGGACCAAA3121.0541252787350497No Hit
TATTCGGGGTCTCTGGATGATAGTATCCCAAACTTCAGCATGACACAATATG2720.9189810122305562No Hit
CTGAACGGGGACTTTCAATACTTCCCCTGAAGGAAGAATGATAAGGAGAAAT2700.9122237989053315No Hit
CTCGAGGGGTGGAGCAGCGCTAGTTCATAATTTAAAAAAATTAAAAAAACGC2670.9020879789174945No Hit
AAGTGCGGGTGAAGCCCTGTTGTGCCACAGAGTGTAGCCAGGTCCCCCTGCA2650.8953307655922697No Hit
TGTCATGGGATTGAGGGTTAAGCTTTCACATGTGAATGTGGGAAAGGACACA2620.8851949456044327No Hit
GGTCTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2350.7939725657138996No Hit
AACTCAGGGTTTGTCCTGTATCTGTAAACACTTTTTGTTCTTAGTCTTTTTC2210.7466720724373268No Hit
TATTATGGGAGGACCAGCAGAGTTTGGGTTGGTAAAAATAATGTTTGCTCTA2200.7432934657747146No Hit
TATTAGGGGATCTCAGATTGACCATTTCTGTTACTTGTCATTTCTACTTTCA2160.7297790391242651No Hit
TGAATGGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA2040.6892357591729171No Hit
AATAAAGGGTCCCATACACAGAAACACAAAACACTTTGATCATAAACTTTTT1950.6588282992094061No Hit
GCCATGGGGTGACATAATGAAATGAAATGATGAAATGGAATAATGAAATGGA1930.6520710858841814No Hit
TTGTCTGGGAGTGGCTAAAGTGGAGCTCTCAGACCACGTGTGTGATGTGGTG1930.6520710858841814No Hit
CCATGGGGGAATAAGAGTTGGCTTAAATTTTAAAAAAGAAAAAGAAAATTGA1840.6216636259206704No Hit
TATCGCGGGATTCTAGGCATGAGAATACCTTAATACTGAGTTGGAGATTTTG1800.608149199270221No Hit
ACAATTGGGCACCCAGACATATTCTCCAACCCAGCATTGGAAATCTGAACGA1800.608149199270221No Hit
ACCAAAGGGGTGCCCCAGGGCGCCCCCTCAGGCTCTCTGAAGAACTGCTGTG1730.5844989526319346No Hit
CTTGTAGGGATAATTTTCCAATCAAAATATTCTATTCTATATTCTAAGGGTT1690.5709845259814853No Hit
AACCATGGGTCTCTGAATAATTAGTTAAGTGCAATTCAGAGCATAATGGTCC1680.5676059193188728No Hit
TACGCCGGTACACTGTTGGTGAGAATGTAAATTAGTGTGATAATTATGGAAA1660.5608487059936482No Hit
TCATTGGGGGGTGGGGCAATGGTTATTTGGTTATTTTACTCAATTGGTTACT1640.5540914926684235No Hit
GAGTAAGGGTAACATGGGAAGAGTAGAAGAGAAAATTAAATGTGGAAATCAT1600.5405770660179742No Hit
GTATTTGGGATAATGGCTGAGGTTAACAGAAAGGGACAGAAAGCTTGGGACT1560.5270626393675248No Hit
CGCTACGGATAACAAACAAGAGATAGCCTTTGAAATGGCAATTGGGAAAAGC1560.5270626393675248No Hit
CGGCGGGATTCATTTTAAATGAAGTAAAAAATGCCTTGAAAGTAATTCTTTA1540.5203054260423001No Hit
AGGTACGGGTACTTGCAAAACTTGTAAATTAGCAGATGACTTTTTTCCATTG1510.5101696060544632No Hit
TTCTGCGGGTGGGCTTCCAGCTGCCTTAATAGAAGTACTCAAGTCTTTTGGG1490.5034123927292385No Hit
ACACACGGGTATCACCAGGAAGTTTGCAAGCTTCTACGAAATAGCCAATAAT1480.5000337860666261No Hit
CCAATAGGGGTTCTAGCATGTGATTACTTCACTCCTGGACTGTGACTTTCAG1460.49327657274140146No Hit
AAAGTAGGGGCCCACCTTGAAGGCTACAATTGAAAATAAAAATTCTGTTCTG1460.49327657274140146No Hit
TTTTCTGGGTTCCTAGAACTCACTTCCTTTGACGGCGTGTGTTGGTCCCATC1410.4763835394283398No Hit
ATGCTAGGGTGATGAGATGAAATGAAATGAAATAATGCAATGAAAGATGAAA1350.4561118994526657No Hit
CAACCAGGTTTCAAAATTTACTGCAAAGCTACTTCAATCAAAACAGTGTTGT1320.44597607946482876No Hit
TGCTAAGGGTAGGTCAATGAAAAAATTAAGAAGAAAATTGAAACTTTTCTGA1320.44597607946482876No Hit
GTTCGCGGGTGTGAAGCAGAAGCTGAGAATTTTATGGCAAAGTAGCTATTCA1300.43921886613960404No Hit
GATAGTGGGTGTGAGTGACAGTATAAAAGATTCACTGTGGCATCGTTTCCAG1280.4324616528143793No Hit
AACGCAGGGATCTCACACAAGTCAAAATGACTGTTATTAAAAAGTCAAAAAA1270.429083046151767No Hit
TGGCACGGAAGCTTGGTCATAATAGATCCAGTGTTTTTCAGATTCCTTTCAC1270.429083046151767No Hit
TTTAATGGGTTGCATGGAAATTAAACAACCTGCTCCTAAGTGACTTTTGGGT1230.4155686195013176No Hit
ATCATAGGGTAGTGAGCTCTTGACTTGATGATGGAGTATTCTTTGTGACTTC1230.4155686195013176No Hit
ACTACAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1220.4121900128387053No Hit
CTCTCGGGGGGACCTGACCAAAATAGGTTATTTTCTCTCTCTGCAAAATACT1220.4121900128387053No Hit
TTTAGTGGGTAAATCTAATGGAATAAAACTTTGAAAAATTTATGTTTTTAAA1210.408811406176093No Hit
TCTCTTGGGAAGACGAGAAACATCTAATTCTACATGAGAAGATGCAAGAAGA1200.4054327995134806No Hit
TCCTATGGGGCTGGTGCTGTGTCTTTATGAATGTTTTAACCATTTTCATGGT1190.4020541928508683No Hit
CCAAGAGGAGGCGCTTCATCTAATTGATGAAATGGATTTGAATGGTGACAAA1180.398675586188256No Hit
GCGCTGGGTAACATTGTTATTCTTAAGCCTTATATAATATTGTAACTTGCAT1100.3716467328873573No Hit
GGCCGTGGGTGTCTGCAAAGACTTCCCATGCTTCAGTCCTTTATGACATTCT1080.36488951956213256No Hit
GTAACAGGGTATGAAAATGACAAAAACACATAGATAAGGAAGATGTTAAAAC1080.36488951956213256No Hit
AATATAGGGAGACTCTTTAACCTGGCTTTTGCCTCTTTCATACCTTGGAACT1060.3581323062369079No Hit
TCTGCAGGGTGGGGATGGGTGCTATTGCCATTTAGAAATAAGAGGCCAGGTA970.3277248462733968No Hit
ATGACAGGGTGATGAAGGGAGGTATCAAAAAGAAGGACATCACAGTCCTAAA960.3243462396107845No Hit
AATTTTGGGGACGCTCTTCCGATCACTCCGGGGTCTGTAGCAACTAGAGAAG930.3142104196229475No Hit
CTACACGCGGGTTGGCTGATTTTTCTCTATCTGGTTATTTTCATTTTCATTC930.3142104196229475No Hit
TTTTTGGGGTCTTTCTCCTTCAATGTTTCAGGTGTCAGTGGCTCCTGTAGTT920.3108318129603352No Hit
GTAAGTGGGATACATTCCCATCCTGTTTATTTTTGGACCTAAGTGTAATGTT910.3074532062977228No Hit
TAAAAGGGATGGAAATTAAGAATGACAATCATAGGGATCAATTCATTTTATT910.3074532062977228No Hit
CACCCAGGGTTTGGGCTTTCCCTCCAGTTCAGAAAGTGACACCAGGAAGAAA900.3040745996351105No Hit
CGTTTGGGGTGAGGCTGGGATGAGACAAGCCCCCGACACGGTTACCAACCAC880.2973173863098858No Hit
TAGACAGGGGGTTCTAATGTATAATAACATTTACCTTCAGCCTCCATCAGAA880.2973173863098858No Hit
CTCACTGGGCTGGTGTGAATTATGAAAACCTTAGTTACACTTGGAAGAGTAA870.29393877964727344No Hit
TAGCTAGGGGACGCTCTTCCGATCTAAATAGGGGAAAATAAAAGAGAAGGAG870.29393877964727344No Hit
CAGGGCAATTTTTCTCTTAATACCAAGGAAGACAGTAGGGAAGAAAGAAGCC860.29056017298466114No Hit
TCTGAGGGGTAACGAGAATCGCTTGTGCCTGGGAGATGGAGGCTGCAGTGAG860.29056017298466114No Hit
AATGCAGGGTGGTGAGAACCTGTCTCTACAAAAAAATATAAAAATTAGCTGT850.2871815663220488No Hit
TGCTAAGGGGGAGTGGCTGGATGATTGCATCTGGTTCCCAAGATAAGACTGT850.2871815663220488No Hit
TATACAGGGAGTGAACCAAGGATCAGTTACGGAAACGATGCTCTCATGCCAT820.27704574633421175No Hit
TGTGCAGGGTATGGTTATGTTACAAATTCATATTCTGTTACTACAGTAAACG780.2635313196837624No Hit
TAAAATGGGTCCGATCCAGGGGGGTGGTATTTCTTTCATTAAATAGCTTTAT770.26015271302115006No Hit
GAAAATGGGGACAAAAAGGGGAATTTCAATTACATCGAGTTCACACGCATCC760.25677410635853776No Hit
TGAAGGGCGATCTACAGGAGGGGGAATACATGCTTCTTCTCTAGAACTGTTT730.24663828637070073No Hit
GTAGTTGGGTACTGTCTCCTAGGAGACAAAAATCATCACTGGCTGAGAACCA720.2432596797080884No Hit
CACTAGGGCACCCGCTCGCTTTTCGTGAACTGAGCCACTCCCTGGGTCACCC700.23650246638286368No Hit
CGTTAAGGGGTTCATAAATGTAATGCATATGATATGTACTTTTAAGTTTTAG690.23312385972025138No Hit
ATCGTAGGGTATATTCAGTGATGCAGTTCTGAACTTGTATATGGCATGATGT690.23312385972025138No Hit
TACCTTGGGTGGAAAGGTTGTCTGCATTAGGGGATGTTTCTGGAAGCATCCC670.22636664639502668No Hit
AGATATGGGATGTCCCTGGCTGTTGAGGCGCTGCTTCAGCCTGCACCCCTCC650.21960943306980202No Hit
AGGCATGGGTATATATATATATATACACACACAAGAACTATTTTCACTGAAA630.21285221974457733No Hit
AGTCAGGGGGACGGGAACAATTCTTAAACTGATTAACTAGCTGTAATATAGT630.21285221974457733No Hit
GCTCGGGGTGAGAATTTAGGGGCATGGTAATTTTTACCAAAACTGAAGCTGG610.20609500641935266No Hit
TAGCCAGGGTCTGAGTAGAAATGCGTAATTTCAATTACTGTATAAAGTTTAT600.2027163997567403No Hit
AGTTGTGGGTGCTGCTTTGACAGTAAAGAGAAGGAAGTATTCTGATTAGCTG590.199337793094128No Hit
GGATATGGGTCCGAGACGAAATCTATGTTCTACCATTACGTTATGAGAATTT590.199337793094128No Hit
TAACTCGGGTCATTCTAGTCTGAATAGGGACAAACAAATCTTGAGGAAGCCC590.199337793094128No Hit
GCATAAGGGTTGAAGGGAAATATTGATGTGCCTGTTTATTGGGGGGTCAGCT580.19595918643151564No Hit
GAGAGGGTTACAGGTGAATATTATGAATTGAGCTCCATTCAATGCACAGATG580.19595918643151564No Hit
TGCTTGGGTAAATGGTGTGGGGAAAGCTAGATAACTATATGCAGAAGAATGA580.19595918643151564No Hit
GGTGCCGGGGTCTGCCTTAAGCAATCTTGGTAGAGCTGTCAATCAGTGGCAG580.19595918643151564No Hit
AAACATGGGTAACCAGAAAGATTATCTTAGACTGTCCTTCACATTATACTTT570.1925805797689033No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAGGACTC570.1925805797689033No Hit
GAATTAGGGTATGGAGTAACTATTTCAGAAAACCCTATAAGAAGTTCATTTT550.18582336644367864No Hit
CAAGCATGGGGCTCTTGTTACCCTCTGCAATAAGTATTTTTTATTATGTATT540.18244475978106628No Hit
TAGCCAGGGGTTCTCCTGTGCCGCTGCATCGCTCTCTCCCGGGAAAGAACAG530.17906615311845395No Hit
TACTAAGGGTGCTCGATGCATTTTCGTCCTGAGAAAAATGTTCTCTACAGAA530.17906615311845395No Hit
CATTCGGGGTGTTCTTTCTATGTATAACTTGACTTTTCAGAAGAAGCTTTGG520.17568754645584161No Hit
ATGCGTGGGTAAACTACTCATGGTCTAAATACTAGAGATAAAGTAGATTCAT520.17568754645584161No Hit
AGCGCAAGGTGGAGGGAGAAATTAGCAAAATCCTCCTTTTCTATAGCTAATA520.17568754645584161No Hit
AGAGTGGGGTGCATTGGGAAAAGAAGTCTGTTAATAGTGATTTAGTCTCCCC520.17568754645584161No Hit
GGTGCTGGGGAGGTACAAAAAAATGCTGCAGATCTAGAAATGCATATATTTA520.17568754645584161No Hit
CATATGGGGATGTAGAATATAGTACACGTTTAAAATAGATTTTATGGAAAAA520.17568754645584161No Hit
TTGGACGGGACTGGTGGATAATTTATGTGATGTGGATTGCTGGTGTCCAGCA510.17230893979322928No Hit
TCGCCAGGGTAGAAACATAAACACCACATAACTACGTGTTCTCACTTATGTA500.16893033313061695No Hit
TCTAACAGGGTGCAGTGACTGACTAGCATTAGGTATGTTTCTTTTATATCCT500.16893033313061695No Hit
CGTAAGGGGATAACAGCATCACTTTAGATTTTGTAAGTCAAAATTGAAATGA500.16893033313061695No Hit
TGCTATGGGCTATTGCTTTGGAGAAAAATTTGAAAAACAATAAATTATGCTT490.1655517264680046No Hit
GAACGCGGGGTAACAATTTACCACACTATGGACCGCATTTAAAAATCAAGAT480.16217311980539226No Hit
CACTCCGGGTAGAAGGGGTGGTGGTGTTTGGATTTGATTTTTTTCAACTTGC470.1587945131427799No Hit
AATTTAGGGTCCTTTCAGCTGTTTCAAACAGGTGCCAGGATACCTATGTCTT460.1554159064801676No Hit
TATGCAGGGTGGTCGCTTTTGCCACAAAATTGTTAATTCCTTCCTGCCGCAC460.1554159064801676No Hit
TCTCTGGGAGTGACATTTCTATTCAGTATTCTGCTGTAAAGAATGTATAACT440.1486586931549429No Hit
ATTACTGGGACTTTCCAAATTGAAGCACTACCGAACCAAAAATCAGAGTGAT440.1486586931549429No Hit
TATAACGGGGGACTTCATCACATGCTTTTAGCATTTGAGAACCTGGCCAGGA440.1486586931549429No Hit
AATGTCGGGGATCTCACTAGGGCTGTATAAATTTGCCTGTTCTAACACCTCA430.14528008649233057No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGGACTCATCCTGTCTCTTATAC430.14528008649233057No Hit
TGTCAAGGGTCACAACTGTTCTTTGAATTTTATGAAAATCCTGGAATATAGG430.14528008649233057No Hit
GGTTGGGGTCAGGAAAACTAAGTTTTTCTCTGCTGTTTTTTTGCTTGAGAGA410.13852287316710588No Hit
TCACAAGGATCCCTGATCCTCCCTGCCCAAGTCTTCCTTCGTGAGCTGGCCT410.13852287316710588No Hit
CGTCTAGGGACTCTTTAAATTTTGAAGTTGACTAGCATTTTCAAACCTTTAT400.13514426650449354No Hit
TAGGTTTGGGTACAGGAGCACCCAGATTCATAAAGCAAGTCCTGAGTGACCT400.13514426650449354No Hit
TTCCAGGGTAAATAGACAGAGTTGACAACACACTCCTGAACAGTCTTTGGAT400.13514426650449354No Hit
TGCACGGGTACAGGCAAGATATGTCTCTTTTAAACAGCACATACAGACACAC390.1317656598418812No Hit
TCGACAGGGGACTTACCTCCAGGAAAGAGAAGGGAAAAGCAATCTCTGGAAA390.1317656598418812No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCAGGACTCATCCTGTCTCT380.12838705317926888No Hit
CATTTTGGGGACGCTCTTCCGATCACTCCGGGGTCTGTAGCAACTAGAGAAG380.12838705317926888No Hit
GGCGGTGGGTGAGCTCTGAACGTCCGTTATAGTTAGGGTGATTGGAAGGTCT370.12500844651665652No Hit
ATCATAGGGGTGGATTAAAGGTCTAAATAGAAAAAACAAAGTTTTAAAGCTG360.1216298398540442No Hit
CATTAAGGGTCTGAGGAGGGTCCTAGAACCAGTGCCCCACAGGTACCAAGGG360.1216298398540442No Hit
TGTAGGGGGAAACCGAAAACCCTATTAAACTCACTCACCTGCCTTTAATGCC360.1216298398540442No Hit
CTAGCCGGGTACAGAAATAACTATACATTTACAACTGATAACATTTGATCAT360.1216298398540442No Hit
GGTCTAGGGGACATGGCTGATAAAGGAGTATTATCCAAAATATACAAAGAAC360.1216298398540442No Hit
GCAAGCTGGGAAATGTATTAAATTATCATAAAAATAAGAAACAAAACAATAA360.1216298398540442No Hit
AGCCAAGGGTCTGCATGGGAATCAATGATTTCTCTACTTCAATGTAGTTTTC350.11825123319143184No Hit
GTTAAAGGGGCTCAGAGTCTGTATCTAGCCTGTGTCAGGATATTGCCTGTTC350.11825123319143184No Hit
GTACATGGGTAGCTGCAGTGAGGAATATAGGCATCTCGTGCTTGGGATTTTG350.11825123319143184No Hit
ACGCATGGGTGAGCTCCCTTTCCTTAGCTTAGAAGAATGTGATTATATCCAG340.11487262652881952No Hit
TTTAGCGGGTGGGCTCTGTCTAGAAACTACAGGTCACAAGAAGCTGGTTTAG330.11149401986620719No Hit
TTTGGAGGGAGCTCCCAGCCTAAGGTCCTATAACCCGAAGCTTGAAGGCAAT330.11149401986620719No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCAGGACTCA320.10811541320359483No Hit
ATATTGGGGTACCATAAACCATATCACATGCCTACATTTTACAAATGATTTC320.10811541320359483No Hit
TGATTCGGGAATGGAAAAAGCACTGAACTAGTATGAGAAACTGGCTCTGAAA320.10811541320359483No Hit
CATCAGGGGTGTGAGGAAAGGATAGAAATGTTCAGGACATCATCATACAGGC310.1047368065409825No Hit
ATAAGCGGGTGGACCTTGTAGTTCCAGCAGTGATGCTGGGCTGCTAAAATAA300.10135819987837015No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)