FastQCFastQC Report
Sat 31 Dec 2022
SRR5844768.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844768.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28944
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCAGGACTCATCTCGTATGC4211.4545328911000552No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGGACTCATCTCGTATGCCGTCT3221.1124930901050303No Hit
TAAAGGAGGGAAAGAGAATTCAAAAAGGGCTGATGGTAGGCTTTGAACATGG3201.105583195135434No Hit
GCGTATGGGGAAAATGCAAAGAGCAAACAAAAGAAAAACAAATAAAAAACAG3151.0883084577114428No Hit
CATCTGGGTCAAAGGAATTTTAACAAAGAGCAATCAGTATTATTGGACCAAA3091.0675787728026533No Hit
TGTCATGGGATTGAGGGTTAAGCTTTCACATGTGAATGTGGGAAAGGACACA2901.001934770591487No Hit
TATTATGGGAGGACCAGCAGAGTTTGGGTTGGTAAAAATAATGTTTGCTCTA2770.95702045328911No Hit
CTCGAGGGGTGGAGCAGCGCTAGTTCATAATTTAAAAAAATTAAAAAAACGC2760.9535655058043118No Hit
TATTCGGGGTCTCTGGATGATAGTATCCCAAACTTCAGCATGACACAATATG2670.9224709784411277No Hit
CTGAACGGGGACTTTCAATACTTCCCCTGAAGGAAGAATGATAAGGAGAAAT2490.8602819237147596No Hit
AAGTGCGGGTGAAGCCCTGTTGTGCCACAGAGTGTAGCCAGGTCCCCCTGCA2460.8499170812603648No Hit
TATTAGGGGATCTCAGATTGACCATTTCTGTTACTTGTCATTTCTACTTTCA2370.8188225538971807No Hit
TGAATGGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA2320.8015478164731896No Hit
AATAAAGGGTCCCATACACAGAAACACAAAACACTTTGATCATAAACTTTTT2090.7220840243228303No Hit
AACTCAGGGTTTGTCCTGTATCTGTAAACACTTTTTGTTCTTAGTCTTTTTC2060.7117191818684355No Hit
GGTCTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2020.6978993919292427No Hit
TTGTCTGGGAGTGGCTAAAGTGGAGCTCTCAGACCACGTGTGTGATGTGGTG1950.6737147595356551No Hit
GCCATGGGGTGACATAATGAAATGAAATGATGAAATGGAATAATGAAATGGA1860.642620232172471No Hit
ACAATTGGGCACCCAGACATATTCTCCAACCCAGCATTGGAAATCTGAACGA1830.6322553897180763No Hit
CCATGGGGGAATAAGAGTTGGCTTAAATTTTAAAAAAGAAAAAGAAAATTGA1810.6253454947484799No Hit
TATCGCGGGATTCTAGGCATGAGAATACCTTAATACTGAGTTGGAGATTTTG1800.6218905472636816No Hit
AACCATGGGTCTCTGAATAATTAGTTAAGTGCAATTCAGAGCATAATGGTCC1790.6184355997788833No Hit
ACCAAAGGGGTGCCCCAGGGCGCCCCCTCAGGCTCTCTGAAGAACTGCTGTG1690.5838861249309011No Hit
CTTGTAGGGATAATTTTCCAATCAAAATATTCTATTCTATATTCTAAGGGTT1680.5804311774461027No Hit
GTATTTGGGATAATGGCTGAGGTTAACAGAAAGGGACAGAAAGCTTGGGACT1670.5769762299613046No Hit
TGCTAAGGGTAGGTCAATGAAAAAATTAAGAAGAAAATTGAAACTTTTCTGA1620.5597014925373134No Hit
GAGTAAGGGTAACATGGGAAGAGTAGAAGAGAAAATTAAATGTGGAAATCAT1560.538971807628524No Hit
CCAAGAGGAGGCGCTTCATCTAATTGATGAAATGGATTTGAATGGTGACAAA1560.538971807628524No Hit
ACACACGGGTATCACCAGGAAGTTTGCAAGCTTCTACGAAATAGCCAATAAT1500.5182421227197347No Hit
GATAGTGGGTGTGAGTGACAGTATAAAAGATTCACTGTGGCATCGTTTCCAG1480.5113322277501382No Hit
GTTCGCGGGTGTGAAGCAGAAGCTGAGAATTTTATGGCAAAGTAGCTATTCA1480.5113322277501382No Hit
CGCTACGGATAACAAACAAGAGATAGCCTTTGAAATGGCAATTGGGAAAAGC1470.50787728026534No Hit
TACGCCGGTACACTGTTGGTGAGAATGTAAATTAGTGTGATAATTATGGAAA1450.5009673852957435No Hit
AGGTACGGGTACTTGCAAAACTTGTAAATTAGCAGATGACTTTTTTCCATTG1440.4975124378109453No Hit
CCAATAGGGGTTCTAGCATGTGATTACTTCACTCCTGGACTGTGACTTTCAG1400.4836926478717523No Hit
ATGCTAGGGTGATGAGATGAAATGAAATGAAATAATGCAATGAAAGATGAAA1390.4802377003869541No Hit
TCATTGGGGGGTGGGGCAATGGTTATTTGGTTATTTTACTCAATTGGTTACT1360.46987285793255945No Hit
TGGCACGGAAGCTTGGTCATAATAGATCCAGTGTTTTTCAGATTCCTTTCAC1350.46641791044776115No Hit
AAAGTAGGGGCCCACCTTGAAGGCTACAATTGAAAATAAAAATTCTGTTCTG1320.45605306799336653No Hit
TTCTGCGGGTGGGCTTCCAGCTGCCTTAATAGAAGTACTCAAGTCTTTTGGG1310.4525981205085683No Hit
ATCATAGGGTAGTGAGCTCTTGACTTGATGATGGAGTATTCTTTGTGACTTC1310.4525981205085683No Hit
CGGCGGGATTCATTTTAAATGAAGTAAAAAATGCCTTGAAAGTAATTCTTTA1290.44568822553897175No Hit
TTTAGTGGGTAAATCTAATGGAATAAAACTTTGAAAAATTTATGTTTTTAAA1270.4387783305693753No Hit
TTTTCTGGGTTCCTAGAACTCACTTCCTTTGACGGCGTGTGTTGGTCCCATC1250.4318684355997789No Hit
ACTACAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1220.42150359314538416No Hit
GGCCGTGGGTGTCTGCAAAGACTTCCCATGCTTCAGTCCTTTATGACATTCT1140.3938640132669984No Hit
TTTAATGGGTTGCATGGAAATTAAACAACCTGCTCCTAAGTGACTTTTGGGT1130.39040906578220014No Hit
TCTCTTGGGAAGACGAGAAACATCTAATTCTACATGAGAAGATGCAAGAAGA1130.39040906578220014No Hit
TGCTAAGGGGGAGTGGCTGGATGATTGCATCTGGTTCCCAAGATAAGACTGT1110.38349917081260365No Hit
AACGCAGGGATCTCACACAAGTCAAAATGACTGTTATTAAAAAGTCAAAAAA1090.3765892758430072No Hit
TAGCTAGGGGACGCTCTTCCGATCTAAATAGGGGAAAATAAAAGAGAAGGAG1080.3731343283582089No Hit
TAAAAGGGATGGAAATTAAGAATGACAATCATAGGGATCAATTCATTTTATT1050.36276948590381425No Hit
TCTGAGGGGTAACGAGAATCGCTTGTGCCTGGGAGATGGAGGCTGCAGTGAG1040.35931453841901606No Hit
GCGCTGGGTAACATTGTTATTCTTAAGCCTTATATAATATTGTAACTTGCAT1020.3524046434494196No Hit
CAACCAGGTTTCAAAATTTACTGCAAAGCTACTTCAATCAAAACAGTGTTGT1000.3454947484798231No Hit
AATATAGGGAGACTCTTTAACCTGGCTTTTGCCTCTTTCATACCTTGGAACT980.33858485351022666No Hit
CTCTCGGGGGGACCTGACCAAAATAGGTTATTTTCTCTCTCTGCAAAATACT980.33858485351022666No Hit
TCCTATGGGGCTGGTGCTGTGTCTTTATGAATGTTTTAACCATTTTCATGGT950.32822001105583193No Hit
CTACACGCGGGTTGGCTGATTTTTCTCTATCTGGTTATTTTCATTTTCATTC940.32476506357103374No Hit
TCTGCAGGGTGGGGATGGGTGCTATTGCCATTTAGAAATAAGAGGCCAGGTA930.3213101160862355No Hit
ATGACAGGGTGATGAAGGGAGGTATCAAAAAGAAGGACATCACAGTCCTAAA900.3109452736318408No Hit
TATACAGGGAGTGAACCAAGGATCAGTTACGGAAACGATGCTCTCATGCCAT900.3109452736318408No Hit
AATTTTGGGGACGCTCTTCCGATCACTCCGGGGTCTGTAGCAACTAGAGAAG880.30403537866224434No Hit
GAAAATGGGGACAAAAAGGGGAATTTCAATTACATCGAGTTCACACGCATCC870.30058043117744615No Hit
TTTTTGGGGTCTTTCTCCTTCAATGTTTCAGGTGTCAGTGGCTCCTGTAGTT850.29367053620784966No Hit
TGTGCAGGGTATGGTTATGTTACAAATTCATATTCTGTTACTACAGTAAACG840.29021558872305137No Hit
GTAAGTGGGATACATTCCCATCCTGTTTATTTTTGGACCTAAGTGTAATGTT830.2867606412382532No Hit
CACCCAGGGTTTGGGCTTTCCCTCCAGTTCAGAAAGTGACACCAGGAAGAAA820.28330569375345493No Hit
GTAACAGGGTATGAAAATGACAAAAACACATAGATAAGGAAGATGTTAAAAC780.269485903814262No Hit
AATGCAGGGTGGTGAGAACCTGTCTCTACAAAAAAATATAAAAATTAGCTGT760.2625760088446656No Hit
CGTTTGGGGTGAGGCTGGGATGAGACAAGCCCCCGACACGGTTACCAACCAC720.24875621890547264No Hit
CACTAGGGCACCCGCTCGCTTTTCGTGAACTGAGCCACTCCCTGGGTCACCC710.24530127142067443No Hit
CTCACTGGGCTGGTGTGAATTATGAAAACCTTAGTTACACTTGGAAGAGTAA680.23493642896627973No Hit
GGTGCCGGGGTCTGCCTTAAGCAATCTTGGTAGAGCTGTCAATCAGTGGCAG680.23493642896627973No Hit
GAATTAGGGTATGGAGTAACTATTTCAGAAAACCCTATAAGAAGTTCATTTT670.23148148148148145No Hit
ATCGTAGGGTATATTCAGTGATGCAGTTCTGAACTTGTATATGGCATGATGT660.22802653399668327No Hit
TACTAAGGGTGCTCGATGCATTTTCGTCCTGAGAAAAATGTTCTCTACAGAA650.22457158651188502No Hit
TAAAATGGGTCCGATCCAGGGGGGTGGTATTTCTTTCATTAAATAGCTTTAT650.22457158651188502No Hit
TGCTTGGGTAAATGGTGTGGGGAAAGCTAGATAACTATATGCAGAAGAATGA650.22457158651188502No Hit
TAGACAGGGGGTTCTAATGTATAATAACATTTACCTTCAGCCTCCATCAGAA630.21766169154228857No Hit
TGAAGGGCGATCTACAGGAGGGGGAATACATGCTTCTTCTCTAGAACTGTTT620.2142067440574903No Hit
CAGGGCAATTTTTCTCTTAATACCAAGGAAGACAGTAGGGAAGAAAGAAGCC600.20729684908789386No Hit
AGTTGTGGGTGCTGCTTTGACAGTAAAGAGAAGGAAGTATTCTGATTAGCTG590.20384190160309562No Hit
TAGCCAGGGTCTGAGTAGAAATGCGTAATTTCAATTACTGTATAAAGTTTAT590.20384190160309562No Hit
CATATGGGGATGTAGAATATAGTACACGTTTAAAATAGATTTTATGGAAAAA580.2003869541182974No Hit
CGTTAAGGGGTTCATAAATGTAATGCATATGATATGTACTTTTAAGTTTTAG570.1969320066334992No Hit
AAACATGGGTAACCAGAAAGATTATCTTAGACTGTCCTTCACATTATACTTT570.1969320066334992No Hit
ATGCGTGGGTAAACTACTCATGGTCTAAATACTAGAGATAAAGTAGATTCAT550.1900221116639027No Hit
TAACTCGGGTCATTCTAGTCTGAATAGGGACAAACAAATCTTGAGGAAGCCC550.1900221116639027No Hit
CGTAAGGGGATAACAGCATCACTTTAGATTTTGTAAGTCAAAATTGAAATGA550.1900221116639027No Hit
GCATAAGGGTTGAAGGGAAATATTGATGTGCCTGTTTATTGGGGGGTCAGCT530.18311221669430625No Hit
GGTGCTGGGGAGGTACAAAAAAATGCTGCAGATCTAGAAATGCATATATTTA530.18311221669430625No Hit
CATTCGGGGTGTTCTTTCTATGTATAACTTGACTTTTCAGAAGAAGCTTTGG520.17965726920950803No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAGGACTC520.17965726920950803No Hit
AGGCATGGGTATATATATATATATACACACACAAGAACTATTTTCACTGAAA510.1762023217247098No Hit
CAAGCATGGGGCTCTTGTTACCCTCTGCAATAAGTATTTTTTATTATGTATT510.1762023217247098No Hit
GGCGGTGGGTGAGCTCTGAACGTCCGTTATAGTTAGGGTGATTGGAAGGTCT510.1762023217247098No Hit
ACTCTGGGGTATACAGAAAGTTGCTAAAGCAAAAAACATAGACACATAACTT490.16929242675511333No Hit
AGCGCAAGGTGGAGGGAGAAATTAGCAAAATCCTCCTTTTCTATAGCTAATA480.16583747927031509No Hit
TATAACGGGGGACTTCATCACATGCTTTTAGCATTTGAGAACCTGGCCAGGA480.16583747927031509No Hit
GTAGTTGGGTACTGTCTCCTAGGAGACAAAAATCATCACTGGCTGAGAACCA480.16583747927031509No Hit
AGCCAAGGGTCTGCATGGGAATCAATGATTTCTCTACTTCAATGTAGTTTTC470.16238253178551687No Hit
AGATATGGGATGTCCCTGGCTGTTGAGGCGCTGCTTCAGCCTGCACCCCTCC470.16238253178551687No Hit
AGTCAGGGGGACGGGAACAATTCTTAAACTGATTAACTAGCTGTAATATAGT470.16238253178551687No Hit
TACCTTGGGTGGAAAGGTTGTCTGCATTAGGGGATGTTTCTGGAAGCATCCC450.1554726368159204No Hit
CTAGCCGGGTACAGAAATAACTATACATTTACAACTGATAACATTTGATCAT450.1554726368159204No Hit
TCGCCAGGGTAGAAACATAAACACCACATAACTACGTGTTCTCACTTATGTA440.15201768933112217No Hit
CATTTTGGGGACGCTCTTCCGATCACTCCGGGGTCTGTAGCAACTAGAGAAG440.15201768933112217No Hit
GGTTGGGGTCAGGAAAACTAAGTTTTTCTCTGCTGTTTTTTTGCTTGAGAGA440.15201768933112217No Hit
TGTCAAGGGTCACAACTGTTCTTTGAATTTTATGAAAATCCTGGAATATAGG440.15201768933112217No Hit
GAGAGGGTTACAGGTGAATATTATGAATTGAGCTCCATTCAATGCACAGATG430.14856274184632393No Hit
TAGCCAGGGGTTCTCCTGTGCCGCTGCATCGCTCTCTCCCGGGAAAGAACAG420.14510779436152568No Hit
GGTCTAGGGGACATGGCTGATAAAGGAGTATTATCCAAAATATACAAAGAAC420.14510779436152568No Hit
TGATTCGGGAATGGAAAAAGCACTGAACTAGTATGAGAAACTGGCTCTGAAA420.14510779436152568No Hit
CATCAGGGGTGTGAGGAAAGGATAGAAATGTTCAGGACATCATCATACAGGC420.14510779436152568No Hit
GCTCGGGGTGAGAATTTAGGGGCATGGTAATTTTTACCAAAACTGAAGCTGG400.13819789939192925No Hit
GTTTATGGGTGTTACATAGGGAAACGTGTGCCTCGGTGGTTTGCTGCACCTA400.13819789939192925No Hit
TATGCAGGGTGGTCGCTTTTGCCACAAAATTGTTAATTCCTTCCTGCCGCAC400.13819789939192925No Hit
CACTCCGGGTAGAAGGGGTGGTGGTGTTTGGATTTGATTTTTTTCAACTTGC400.13819789939192925No Hit
GCATTTGGGGCAGTGGGCTGGGCGCGGTGGCTCATGCCTGTAATCCCGGCAC390.134742951907131No Hit
TTGGACGGGACTGGTGGATAATTTATGTGATGTGGATTGCTGGTGTCCAGCA390.134742951907131No Hit
TCACAAGGATCCCTGATCCTCCCTGCCCAAGTCTTCCTTCGTGAGCTGGCCT390.134742951907131No Hit
TGTAGGGGGAAACCGAAAACCCTATTAAACTCACTCACCTGCCTTTAATGCC370.12783305693753455No Hit
TCTAACAGGGTGCAGTGACTGACTAGCATTAGGTATGTTTCTTTTATATCCT370.12783305693753455No Hit
TGCTATGGGCTATTGCTTTGGAGAAAAATTTGAAAAACAATAAATTATGCTT370.12783305693753455No Hit
AGAGTGGGGTGCATTGGGAAAAGAAGTCTGTTAATAGTGATTTAGTCTCCCC370.12783305693753455No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGGACTCATCCTGTCTCTTATAC370.12783305693753455No Hit
GAATCTGGGGATCTTGCAATGGGAAACCCATGCTGGAAACAACCCAAATGTC360.12437810945273632No Hit
GTTAAAGGGGCTCAGAGTCTGTATCTAGCCTGTGTCAGGATATTGCCTGTTC350.12092316196793808No Hit
AAATGCGGGTAATCACCAAATTTAAAGAGAATACATGTATTTTCTAGCATAA350.12092316196793808No Hit
GAACGCGGGGTAACAATTTACCACACTATGGACCGCATTTAAAAATCAAGAT340.11746821448313986No Hit
TCGACAGGGGACTTACCTCCAGGAAAGAGAAGGGAAAAGCAATCTCTGGAAA340.11746821448313986No Hit
TAGGTTTGGGTACAGGAGCACCCAGATTCATAAAGCAAGTCCTGAGTGACCT330.11401326699834163No Hit
ATCATAGGGGTGGATTAAAGGTCTAAATAGAAAAAACAAAGTTTTAAAGCTG320.11055831951354339No Hit
TGCACGGGTACAGGCAAGATATGTCTCTTTTAAACAGCACATACAGACACAC320.11055831951354339No Hit
TAGCTGGGACTTCTCTCTGTATTTTTTCTTGCATTGACATTATAGACATTGA320.11055831951354339No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCAGGACTCATCCTGTCTCT320.11055831951354339No Hit
ATTACTGGGACTTTCCAAATTGAAGCACTACCGAACCAAAAATCAGAGTGAT320.11055831951354339No Hit
CGTCTAGGGACTCTTTAAATTTTGAAGTTGACTAGCATTTTCAAACCTTTAT310.10710337202874515No Hit
GTACATGGGTAGCTGCAGTGAGGAATATAGGCATCTCGTGCTTGGGATTTTG310.10710337202874515No Hit
AATTTAGGGTCCTTTCAGCTGTTTCAAACAGGTGCCAGGATACCTATGTCTT310.10710337202874515No Hit
GAGGCAGGAGAATCGCTTGAACCCGGGAGGCGGAGGTTGCAGTGAGCCGAGA310.10710337202874515No Hit
GGATATGGGTCCGAGACGAAATCTATGTTCTACCATTACGTTATGAGAATTT310.10710337202874515No Hit
TGATCAGGGTAGTGGTGCATTAGTTTAGAAAGTCAGCTATGATTTTGCCTAT310.10710337202874515No Hit
GCAACTGGGAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAAT300.10364842454394693No Hit
AACTCGGGTCCTCTTAGAAGTGAACTGATTGCACTGGATCCCTAAACCCACA290.1001934770591487No Hit
AAGCTAGGGGACGCTCTTCCGATCTAAATAGGGGAAAATAAAAGAGAAGGAG290.1001934770591487No Hit
TTCCAGGGTAAATAGACAGAGTTGACAACACACTCCTGAACAGTCTTTGGAT290.1001934770591487No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)