FastQCFastQC Report
Sat 31 Dec 2022
SRR5844767.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844767.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29245
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCAGGACTCATCTCGTATGC4221.442981706274577No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGGACTCATCTCGTATGCCGTCT3771.2891092494443495No Hit
TAAAGGAGGGAAAGAGAATTCAAAAAGGGCTGATGGTAGGCTTTGAACATGG3241.107881689177637No Hit
CTGAACGGGGACTTTCAATACTTCCCCTGAAGGAAGAATGATAAGGAGAAAT3111.0634296460933492No Hit
GCGTATGGGGAAAATGCAAAGAGCAAACAAAAGAAAAACAAATAAAAAACAG2981.0189776030090614No Hit
CATCTGGGTCAAAGGAATTTTAACAAAGAGCAATCAGTATTATTGGACCAAA2981.0189776030090614No Hit
TATTATGGGAGGACCAGCAGAGTTTGGGTTGGTAAAAATAATGTTTGCTCTA2780.9505898444178491No Hit
CTCGAGGGGTGGAGCAGCGCTAGTTCATAATTTAAAAAAATTAAAAAAACGC2780.9505898444178491No Hit
TGTCATGGGATTGAGGGTTAAGCTTTCACATGTGAATGTGGGAAAGGACACA2630.8992990254744401No Hit
TATTCGGGGTCTCTGGATGATAGTATCCCAAACTTCAGCATGACACAATATG2300.78645922379894No Hit
AACTCAGGGTTTGTCCTGTATCTGTAAACACTTTTTGTTCTTAGTCTTTTTC2270.7762010600102582No Hit
TATTAGGGGATCTCAGATTGACCATTTCTGTTACTTGTCATTTCTACTTTCA2140.7317490169259703No Hit
GGTCTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2130.7283296289964096No Hit
AAGTGCGGGTGAAGCCCTGTTGTGCCACAGAGTGTAGCCAGGTCCCCCTGCA2010.6872969738416824No Hit
AATAAAGGGTCCCATACACAGAAACACAAAACACTTTGATCATAAACTTTTT2000.6838775859121217No Hit
ACAATTGGGCACCCAGACATATTCTCCAACCCAGCATTGGAAATCTGAACGA1990.6804581979825611No Hit
CCATGGGGGAATAAGAGTTGGCTTAAATTTTAAAAAAGAAAAAGAAAATTGA1920.6565224824756368No Hit
TGAATGGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1910.6531030945460763No Hit
GCCATGGGGTGACATAATGAAATGAAATGATGAAATGGAATAATGAAATGGA1900.6496837066165156No Hit
TATCGCGGGATTCTAGGCATGAGAATACCTTAATACTGAGTTGGAGATTTTG1850.6325867669687126No Hit
ACACACGGGTATCACCAGGAAGTTTGCAAGCTTCTACGAAATAGCCAATAAT1790.612070439391349No Hit
AACCATGGGTCTCTGAATAATTAGTTAAGTGCAATTCAGAGCATAATGGTCC1730.5915541118139853No Hit
TTCTGCGGGTGGGCTTCCAGCTGCCTTAATAGAAGTACTCAAGTCTTTTGGG1720.5881347238844247No Hit
ACCAAAGGGGTGCCCCAGGGCGCCCCCTCAGGCTCTCTGAAGAACTGCTGTG1670.5710377842366217No Hit
GAGTAAGGGTAACATGGGAAGAGTAGAAGAGAAAATTAAATGTGGAAATCAT1660.5676183963070611No Hit
TTGTCTGGGAGTGGCTAAAGTGGAGCTCTCAGACCACGTGTGTGATGTGGTG1660.5676183963070611No Hit
CTTGTAGGGATAATTTTCCAATCAAAATATTCTATTCTATATTCTAAGGGTT1650.5641990083775005No Hit
CGGCGGGATTCATTTTAAATGAAGTAAAAAATGCCTTGAAAGTAATTCTTTA1610.550521456659258No Hit
TCATTGGGGGGTGGGGCAATGGTTATTTGGTTATTTTACTCAATTGGTTACT1590.5436826808001368No Hit
GTATTTGGGATAATGGCTGAGGTTAACAGAAAGGGACAGAAAGCTTGGGACT1590.5436826808001368No Hit
TACGCCGGTACACTGTTGGTGAGAATGTAAATTAGTGTGATAATTATGGAAA1540.5265857411523338No Hit
TGCTAAGGGTAGGTCAATGAAAAAATTAAGAAGAAAATTGAAACTTTTCTGA1530.5231663532227732No Hit
CGCTACGGATAACAAACAAGAGATAGCCTTTGAAATGGCAATTGGGAAAAGC1520.5197469652932125No Hit
TTTTCTGGGTTCCTAGAACTCACTTCCTTTGACGGCGTGTGTTGGTCCCATC1520.5197469652932125No Hit
CCAAGAGGAGGCGCTTCATCTAATTGATGAAATGGATTTGAATGGTGACAAA1490.5094888015045307No Hit
ATGCTAGGGTGATGAGATGAAATGAAATGAAATAATGCAATGAAAGATGAAA1480.50606941357497No Hit
ATCATAGGGTAGTGAGCTCTTGACTTGATGATGGAGTATTCTTTGTGACTTC1460.49923063771584886No Hit
AGGTACGGGTACTTGCAAAACTTGTAAATTAGCAGATGACTTTTTTCCATTG1420.48555308599760644No Hit
AAAGTAGGGGCCCACCTTGAAGGCTACAATTGAAAATAAAAATTCTGTTCTG1400.47871431013848514No Hit
GATAGTGGGTGTGAGTGACAGTATAAAAGATTCACTGTGGCATCGTTTCCAG1360.4650367584202428No Hit
GTTCGCGGGTGTGAAGCAGAAGCTGAGAATTTTATGGCAAAGTAGCTATTCA1360.4650367584202428No Hit
ACTACAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1280.43768165498375794No Hit
CCAATAGGGGTTCTAGCATGTGATTACTTCACTCCTGGACTGTGACTTTCAG1250.42742349119507606No Hit
CTCTCGGGGGGACCTGACCAAAATAGGTTATTTTCTCTCTCTGCAAAATACT1230.4205847153359549No Hit
TCTCTTGGGAAGACGAGAAACATCTAATTCTACATGAGAAGATGCAAGAAGA1220.4171653274063942No Hit
TGCTAAGGGGGAGTGGCTGGATGATTGCATCTGGTTCCCAAGATAAGACTGT1210.41374593947683364No Hit
CAACCAGGTTTCAAAATTTACTGCAAAGCTACTTCAATCAAAACAGTGTTGT1170.40006838775859127No Hit
GCGCTGGGTAACATTGTTATTCTTAAGCCTTATATAATATTGTAACTTGCAT1150.39322961189947No Hit
CTACACGCGGGTTGGCTGATTTTTCTCTATCTGGTTATTTTCATTTTCATTC1140.3898102239699094No Hit
TCTGAGGGGTAACGAGAATCGCTTGTGCCTGGGAGATGGAGGCTGCAGTGAG1110.37955206018122756No Hit
TGGCACGGAAGCTTGGTCATAATAGATCCAGTGTTTTTCAGATTCCTTTCAC1080.36929389639254573No Hit
AATATAGGGAGACTCTTTAACCTGGCTTTTGCCTCTTTCATACCTTGGAACT1060.3624551205334245No Hit
TGTGCAGGGTATGGTTATGTTACAAATTCATATTCTGTTACTACAGTAAACG1050.3590357326038639No Hit
TAAAAGGGATGGAAATTAAGAATGACAATCATAGGGATCAATTCATTTTATT1050.3590357326038639No Hit
TCCTATGGGGCTGGTGCTGTGTCTTTATGAATGTTTTAACCATTTTCATGGT1050.3590357326038639No Hit
GGCCGTGGGTGTCTGCAAAGACTTCCCATGCTTCAGTCCTTTATGACATTCT1040.3556163446743033No Hit
AACGCAGGGATCTCACACAAGTCAAAATGACTGTTATTAAAAAGTCAAAAAA1040.3556163446743033No Hit
TTTAGTGGGTAAATCTAATGGAATAAAACTTTGAAAAATTTATGTTTTTAAA1030.3521969567447427No Hit
GTAAGTGGGATACATTCCCATCCTGTTTATTTTTGGACCTAAGTGTAATGTT970.33168062916737906No Hit
TAGACAGGGGGTTCTAATGTATAATAACATTTACCTTCAGCCTCCATCAGAA970.33168062916737906No Hit
CTCACTGGGCTGGTGTGAATTATGAAAACCTTAGTTACACTTGGAAGAGTAA970.33168062916737906No Hit
TCTGCAGGGTGGGGATGGGTGCTATTGCCATTTAGAAATAAGAGGCCAGGTA960.3282612412378184No Hit
TTTAATGGGTTGCATGGAAATTAAACAACCTGCTCCTAAGTGACTTTTGGGT950.3248418533082578No Hit
ATGACAGGGTGATGAAGGGAGGTATCAAAAAGAAGGACATCACAGTCCTAAA950.3248418533082578No Hit
GAAAATGGGGACAAAAAGGGGAATTTCAATTACATCGAGTTCACACGCATCC950.3248418533082578No Hit
CACTAGGGCACCCGCTCGCTTTTCGTGAACTGAGCCACTCCCTGGGTCACCC930.3180030774491366No Hit
GTAACAGGGTATGAAAATGACAAAAACACATAGATAAGGAAGATGTTAAAAC900.3077449136604548No Hit
TATACAGGGAGTGAACCAAGGATCAGTTACGGAAACGATGCTCTCATGCCAT880.3009061378013335No Hit
CACCCAGGGTTTGGGCTTTCCCTCCAGTTCAGAAAGTGACACCAGGAAGAAA870.2974867498717729No Hit
TAGCTAGGGGACGCTCTTCCGATCTAAATAGGGGAAAATAAAAGAGAAGGAG850.29064797401265174No Hit
AATTTTGGGGACGCTCTTCCGATCACTCCGGGGTCTGTAGCAACTAGAGAAG840.28722858608309115No Hit
TACCTTGGGTGGAAAGGTTGTCTGCATTAGGGGATGTTTCTGGAAGCATCCC780.2667122585057275No Hit
TGAAGGGCGATCTACAGGAGGGGGAATACATGCTTCTTCTCTAGAACTGTTT770.2632928705761669No Hit
TGCTTGGGTAAATGGTGTGGGGAAAGCTAGATAACTATATGCAGAAGAATGA750.2564540947170456No Hit
GTAGTTGGGTACTGTCTCCTAGGAGACAAAAATCATCACTGGCTGAGAACCA750.2564540947170456No Hit
CGTTTGGGGTGAGGCTGGGATGAGACAAGCCCCCGACACGGTTACCAACCAC740.253034706787485No Hit
TTTTTGGGGTCTTTCTCCTTCAATGTTTCAGGTGTCAGTGGCTCCTGTAGTT730.24961531885792443No Hit
GAATTAGGGTATGGAGTAACTATTTCAGAAAACCCTATAAGAAGTTCATTTT730.24961531885792443No Hit
AATGCAGGGTGGTGAGAACCTGTCTCTACAAAAAAATATAAAAATTAGCTGT710.24277654299880322No Hit
TAGCCAGGGTCTGAGTAGAAATGCGTAATTTCAATTACTGTATAAAGTTTAT710.24277654299880322No Hit
CAGGGCAATTTTTCTCTTAATACCAAGGAAGACAGTAGGGAAGAAAGAAGCC670.22909899128056077No Hit
ATCGTAGGGTATATTCAGTGATGCAGTTCTGAACTTGTATATGGCATGATGT650.2222602154214396No Hit
TAAAATGGGTCCGATCCAGGGGGGTGGTATTTCTTTCATTAAATAGCTTTAT640.21884082749187897No Hit
CGTTAAGGGGTTCATAAATGTAATGCATATGATATGTACTTTTAAGTTTTAG630.21542143956231832No Hit
AAACATGGGTAACCAGAAAGATTATCTTAGACTGTCCTTCACATTATACTTT630.21542143956231832No Hit
GCATAAGGGTTGAAGGGAAATATTGATGTGCCTGTTTATTGGGGGGTCAGCT610.2085826637031971No Hit
TCACAAGGATCCCTGATCCTCCCTGCCCAAGTCTTCCTTCGTGAGCTGGCCT610.2085826637031971No Hit
GGATATGGGTCCGAGACGAAATCTATGTTCTACCATTACGTTATGAGAATTT600.20516327577363652No Hit
AGCGCAAGGTGGAGGGAGAAATTAGCAAAATCCTCCTTTTCTATAGCTAATA580.1983244999145153No Hit
AGAGTGGGGTGCATTGGGAAAAGAAGTCTGTTAATAGTGATTTAGTCTCCCC580.1983244999145153No Hit
GGTGCCGGGGTCTGCCTTAAGCAATCTTGGTAGAGCTGTCAATCAGTGGCAG580.1983244999145153No Hit
TAACTCGGGTCATTCTAGTCTGAATAGGGACAAACAAATCTTGAGGAAGCCC570.1949051119849547No Hit
CAAGCATGGGGCTCTTGTTACCCTCTGCAATAAGTATTTTTTATTATGTATT570.1949051119849547No Hit
CATATGGGGATGTAGAATATAGTACACGTTTAAAATAGATTTTATGGAAAAA570.1949051119849547No Hit
TTGGACGGGACTGGTGGATAATTTATGTGATGTGGATTGCTGGTGTCCAGCA550.18806633612583348No Hit
ATGCGTGGGTAAACTACTCATGGTCTAAATACTAGAGATAAAGTAGATTCAT540.18464694819627286No Hit
AGATATGGGATGTCCCTGGCTGTTGAGGCGCTGCTTCAGCCTGCACCCCTCC540.18464694819627286No Hit
GGTGCTGGGGAGGTACAAAAAAATGCTGCAGATCTAGAAATGCATATATTTA520.17780817233715165No Hit
CACTCCGGGTAGAAGGGGTGGTGGTGTTTGGATTTGATTTTTTTCAACTTGC520.17780817233715165No Hit
GAGAGGGTTACAGGTGAATATTATGAATTGAGCTCCATTCAATGCACAGATG510.17438878440759104No Hit
AATTTAGGGTCCTTTCAGCTGTTTCAAACAGGTGCCAGGATACCTATGTCTT510.17438878440759104No Hit
ATTACTGGGACTTTCCAAATTGAAGCACTACCGAACCAAAAATCAGAGTGAT510.17438878440759104No Hit
AGGCATGGGTATATATATATATATACACACACAAGAACTATTTTCACTGAAA500.17096939647803042No Hit
GCTCGGGGTGAGAATTTAGGGGCATGGTAATTTTTACCAAAACTGAAGCTGG490.16755000854846983No Hit
AGTTGTGGGTGCTGCTTTGACAGTAAAGAGAAGGAAGTATTCTGATTAGCTG490.16755000854846983No Hit
TAGGTTTGGGTACAGGAGCACCCAGATTCATAAAGCAAGTCCTGAGTGACCT480.1641306206189092No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAGGACTC480.1641306206189092No Hit
TAGCCAGGGGTTCTCCTGTGCCGCTGCATCGCTCTCTCCCGGGAAAGAACAG470.1607112326893486No Hit
AGTCAGGGGGACGGGAACAATTCTTAAACTGATTAACTAGCTGTAATATAGT460.157291844759788No Hit
TATGCAGGGTGGTCGCTTTTGCCACAAAATTGTTAATTCCTTCCTGCCGCAC450.1538724568302274No Hit
TATAACGGGGGACTTCATCACATGCTTTTAGCATTTGAGAACCTGGCCAGGA450.1538724568302274No Hit
CACAGGGGGCGATCTGTGAATGGGTCAATACATAACATTGTTTATGTTTACA440.15045306890066676No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCAGGACTCATCCTGTCTCT440.15045306890066676No Hit
GGTTGGGGTCAGGAAAACTAAGTTTTTCTCTGCTGTTTTTTTGCTTGAGAGA440.15045306890066676No Hit
CGTCTAGGGACTCTTTAAATTTTGAAGTTGACTAGCATTTTCAAACCTTTAT430.14703368097110617No Hit
TACTAAGGGTGCTCGATGCATTTTCGTCCTGAGAAAAATGTTCTCTACAGAA430.14703368097110617No Hit
GAGGCAGGAGAATCGCTTGAACCCGGGAGGCGGAGGTTGCAGTGAGCCGAGA420.14361429304154558No Hit
TGTCAAGGGTCACAACTGTTCTTTGAATTTTATGAAAATCCTGGAATATAGG420.14361429304154558No Hit
AAATGCGGGTAATCACCAAATTTAAAGAGAATACATGTATTTTCTAGCATAA410.14019490511198496No Hit
GGCGGTGGGTGAGCTCTGAACGTCCGTTATAGTTAGGGTGATTGGAAGGTCT410.14019490511198496No Hit
TCTAACAGGGTGCAGTGACTGACTAGCATTAGGTATGTTTCTTTTATATCCT400.13677551718242434No Hit
TCTCTGGGAGTGACATTTCTATTCAGTATTCTGCTGTAAAGAATGTATAACT390.13335612925286375No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGGACTCATCCTGTCTCTTATAC390.13335612925286375No Hit
GTTAAAGGGGCTCAGAGTCTGTATCTAGCCTGTGTCAGGATATTGCCTGTTC380.12993674132330313No Hit
CATTCGGGGTGTTCTTTCTATGTATAACTTGACTTTTCAGAAGAAGCTTTGG380.12993674132330313No Hit
TTCCAGGGTAAATAGACAGAGTTGACAACACACTCCTGAACAGTCTTTGGAT380.12993674132330313No Hit
CGTAAGGGGATAACAGCATCACTTTAGATTTTGTAAGTCAAAATTGAAATGA380.12993674132330313No Hit
ACTCTGGGGTATACAGAAAGTTGCTAAAGCAAAAAACATAGACACATAACTT370.1265173533937425No Hit
CATTTTGGGGACGCTCTTCCGATCACTCCGGGGTCTGTAGCAACTAGAGAAG370.1265173533937425No Hit
CATTAAGGGTCTGAGGAGGGTCCTAGAACCAGTGCCCCACAGGTACCAAGGG360.12309796546418192No Hit
ACGCATGGGTGAGCTCCCTTTCCTTAGCTTAGAAGAATGTGATTATATCCAG360.12309796546418192No Hit
ATCATAGGGGTGGATTAAAGGTCTAAATAGAAAAAACAAAGTTTTAAAGCTG350.11967857753462129No Hit
GGTCTAGGGGACATGGCTGATAAAGGAGTATTATCCAAAATATACAAAGAAC350.11967857753462129No Hit
TGCTATGGGCTATTGCTTTGGAGAAAAATTTGAAAAACAATAAATTATGCTT350.11967857753462129No Hit
TGATTCGGGAATGGAAAAAGCACTGAACTAGTATGAGAAACTGGCTCTGAAA350.11967857753462129No Hit
AATGTCGGGGATCTCACTAGGGCTGTATAAATTTGCCTGTTCTAACACCTCA340.1162591896050607No Hit
TCGCCAGGGTAGAAACATAAACACCACATAACTACGTGTTCTCACTTATGTA340.1162591896050607No Hit
TGCAGAGGGCGGAGAGCCGGGATCACGTCACTGCACTCCCCCCTGGGTTATA340.1162591896050607No Hit
TGCACGGGTACAGGCAAGATATGTCTCTTTTAAACAGCACATACAGACACAC330.11283980167550009No Hit
GCAAGCTGGGAAATGTATTAAATTATCATAAAAATAAGAAACAAAACAATAA330.11283980167550009No Hit
TTTGGAGGGAGCTCCCAGCCTAAGGTCCTATAACCCGAAGCTTGAAGGCAAT330.11283980167550009No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCAGGACTCA320.10942041374593948No Hit
CTAGCCGGGTACAGAAATAACTATACATTTACAACTGATAACATTTGATCAT320.10942041374593948No Hit
TCGACAGGGGACTTACCTCCAGGAAAGAGAAGGGAAAAGCAATCTCTGGAAA320.10942041374593948No Hit
AGCCAAGGGTCTGCATGGGAATCAATGATTTCTCTACTTCAATGTAGTTTTC300.10258163788681826No Hit
TTCTCAGGGGAATTTTATGACGTGAAGTATATCTCAATAAAGATGCTAAAAA300.10258163788681826No Hit
TGATCAGGGTAGTGGTGCATTAGTTTAGAAAGTCAGCTATGATTTTGCCTAT300.10258163788681826No Hit
ATGTACGGGTGTCTCTCCTTTTCTGAACCATTAAATATGACTGTTTGCCAAT300.10258163788681826No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)