FastQCFastQC Report
Sat 31 Dec 2022
SRR5844764.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844764.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165048
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGGACTCATCTCGTATGCCGTCT5100.30900101788570594No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCAGGACTCATCTCGTATGC4480.27143618826038485No Hit
CAGATAGGGTAAACTCAAATCCAAAAATATACAATGCATACACAAAAAGTAG2110.1278415975958509No Hit
ATCAAAGGGGGAAGTTATCAGAAGAAACATAGTGGCATAAAAACTGTGCAAG2060.1248121758518734No Hit
TAGCTTGGGATTCAGGTAAAACAATGAATGAGCCACCTTAGCAGGAGCAGAT2050.1242062915030779No Hit
TTGCTAGGGGTATGCTTTTCAAATGCTATCTGCCGTGAATTTTTTGCTTTTA2020.12238863845669139No Hit
GCTAAAGGGGTGTCTACTTAGCTCGCATTTCTCAGTGTTTGTCAGTTTCTCT2020.12238863845669139No Hit
CCACAAGGGATTCACTAATGACAAATTCCAGGTCAGCACAAATAAATTGACG2010.12178275410789588No Hit
CATTACGGGATTGAAAGTTATGTTTCTTTGTTTACTTCATTTTTTCCTCTAA2000.12117686975910039No Hit
AGCCAAGGGCATCCCTTGTGATATCATGGGCCACTTTTAGTCTTTTATTTGG1970.11935921671271388No Hit
GTACATGGGATGCTTTATAAATGAATTTGATGAATGGCAGTTTTTATATTTT1950.11814744801512288No Hit
AAGCTGGGGACTCTTGAGGTAGAACAGTGAACAATTACTCAAAAATCAGAAA1940.11754156366632737No Hit
CAGGTGGGGGCCTCGCGGATGGCGGCGCAGGGCGTAGGGCCTGGGCCGGGGT1930.11693567931753188No Hit
GCGGCCGGGGCAAATTTAAAATGATTGACTTTTATTTTGCAGTTAGCAAATA1910.11572391061994086No Hit
GAGAATGGGCAATTTAGAAATACCTACTTCTAAATAAATATTTGTAATGTGC1900.11511802627114537No Hit
GACACCGGGATTCAGCCTCAGTAGTAGCGAACTGCACTGCTGTTTGGTTTGG1890.11451214192234986No Hit
CTGCTTGGGACGACCAGTGAAATCCAGCATCTTTCAAGTGTTTATTGGCCCT1880.11390625757355435No Hit
CACATTGGGGGATCACTACCGCTACTGAGAGCCAGGTCTGCGTTTCACCCTC1860.11269448887596335No Hit
ATTGCGGGGCCAGGCTCCGTTGTAGGGGTTACAGAACAGTCGTTCCCGCCTC1860.11269448887596335No Hit
TAAAACCCCAGCTAAAATCAGAAAGCTCTACCTCTGCATTCTCTACACCTAC1850.11208860452716785No Hit
CCGGGTGGGGTTACAGATTAGTCTTAAAGAATTGCATTAACTTAAAAAAAAT1840.11148272017837235No Hit
AGTCCAGGGAAGGTGAGGACAATAAAATGCTATCTCTTAGCAATTGATTAGT1830.11087683582957684No Hit
TAAGCCGGGTGCTAGAAAGTGGCAGTTCAGTGGGAGACGAGACCGATCTTGC1830.11087683582957684No Hit
TCAGCAGGGAATCTAAGCATGAGGAAGCCATCGGTCAGGTCCTCAACGGAGG1830.11087683582957684No Hit
TTTGAGGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAACA1820.11027095148078135No Hit
GTGCTGGGGGAATAAAGCAAAAATATTAACATAAAATGACTGAGTTACTGGA1820.11027095148078135No Hit
GTCATAGGGGAGGTTTCGTCTTCCCGGAAGCGTTGGAGGACATTCCCTGTTG1820.11027095148078135No Hit
CAAACAGGGGATCTTTACCAGGGTGGAAAACAGAATAAAAACAAACTTGGTA1820.11027095148078135No Hit
CGTATGGGGGAATAGTTTTTGAACTTCTGAAGAAATAAAAGTTAAGCTGGTG1810.10966506713198584No Hit
TCGTTAGGGGTTCAGATTGGCAGTCTCTTTCGCTTATCTGCTGTTTTAAAGA1810.10966506713198584No Hit
TGGTTTGGTAACAGGCATTGCTATTATTTTACTTTTCACTATGAAACAGATA1810.10966506713198584No Hit
AAAGTAGGGAGGCGGAGGGGAACCAGCAGCTTTGGACGTTATTGATTGTCTG1790.10845329843439484No Hit
TCTCACGGATGACAATATCTATTTACTATATCTTTGTTAGCCCTGAGTACTA1790.10845329843439484No Hit
GGTCGCGGATGACAAAGATTCTCTGAGTGAAGTTAACACCACGTATTTTACC1790.10845329843439484No Hit
GTAAACGGGGGGGAAAAATAAAGGAAATGTGTCTAGTGAATCTGGTTTTAAT1790.10845329843439484No Hit
AATACGGGGAACCCGGACCTCTGACGTTGTTGGGAGGTGGCTGTTACCCACA1790.10845329843439484No Hit
GGTTCTGGGCCGGCAGAGATGGGGTTTATGTATGTTCAGCCAGATAGTAAAT1780.10784741408559934No Hit
GCTTTTGGGGAAGACTTAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGT1780.10784741408559934No Hit
GCTGGGGTGGAAGCTACCTCTGCAGCATTCCTGGTATTCCTTGAAAATCTCC1770.10724152973680384No Hit
ACACATGGGAATCAATTTTATACATAAGAATAGCTTCAGCTTCCTTAGAAAT1770.10724152973680384No Hit
CAACAACGGATGCCCAGTGTGGATAAAATGTCACATTTCTGTAACTTTTGAC1760.10663564538800833No Hit
TTCAGCGGGCAAACTTTGGAGAAAGTTGTCATCTTCAGAGTATATAACTGCT1760.10663564538800833No Hit
GGCTTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1760.10663564538800833No Hit
CTGTCTAGAACTGTCAAAACAGCCTCAGGCAGGTTCTTCAGGAGCTATTCTA1750.10602976103921283No Hit
CGGTTCGGGGAAAATAACATTTTTGTTTTTCTCAGCTTCCAAAGCAGTTCGT1750.10602976103921283No Hit
AAAAGGGCTTTGGAAAAAAACAACTTTTAGTTTCACTGATATTTTTCCATTG1740.10542387669041733No Hit
TCAGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1740.10542387669041733No Hit
AGCGTGGGAAAGTACAATTTCAAAGGTCATTACCAACAGCAGGGTTTTTTTT1730.10481799234162183No Hit
GTGCAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1730.10481799234162183No Hit
ACTAATGGGCTGGAATACCAATACATTCTACAAAGCATTCAGTTGATTTTCC1730.10481799234162183No Hit
CAGCCTGGGCAACAGAGCAAGATTGTCTCAAAAAGAAAAACAGTATTGATAA1720.10421210799282632No Hit
GGTTTATGGGCAGTTTAGAAAAAGCAGAACTAGACACAGGATGCTCATGTAC1720.10421210799282632No Hit
TTATGTGGGGTTTTCTATACTTTTTTCCATAATGGCTGTACCAATTTACATT1710.10360622364403083No Hit
TTCCGGGAGTAGAATAATGTAAATGTCAGAACCTCCTGGGTTGTGTTTATCT1710.10360622364403083No Hit
GCGCGCGGGACAATTGTTAAAACTATAATTTTAATTCTCCTATTTAGAGCAT1710.10360622364403083No Hit
GCATATGGGTAAGTGGAAAAGGATCACTATTATGAATCATAGGTGTTACATA1710.10360622364403083No Hit
TAGCCGGGCAACTGGAAGAGGGTCCTAGCAGTCAATCCGGGCCTTGGGCACA1710.10360622364403083No Hit
GTTCCCCAAAGCGGCAGAGCTGTACGAGCGCGTGGCCTCCAACCGTGAGCAC1710.10360622364403083No Hit
TAGTCGCGGGCTATACATTTCCTTCTGAGTGCTGATTTTCTGTCCAGAAGCC1710.10360622364403083No Hit
AAGATGGGGTAATCGATGAGATGTCTCGATCTAAGGCATCTTAGCACCAATA1700.10300033929523532No Hit
TAGCCAAGGGGAAGTGAGCGGCGGGGTCGACTGACGGTAACGGGGCAGAGAG1700.10300033929523532No Hit
TAGATAGGGGAGCCCGAAGCACCTGACATGAGGGCGGGGACCCCGAAATGCA1700.10300033929523532No Hit
AGGCATGGGAGATGTCTCTGGGAAAACTGCTTTAAATCAAGCTATATTGCAA1700.10300033929523532No Hit
CTCAGCGGGGCGGGCTGTGTGCAGGAAACATGAACTTATGCAGTATGACTTG1700.10300033929523532No Hit
ACCGTGGGGCAGAGACCAGTCTCCATGCTTTTTCTACCACTTAGGGAAAAAC1700.10300033929523532No Hit
AGATTAGGGCAGGTGGCCAGATACCGCCCACGTGCCCCCATCATTGCTGTGA1680.10178857059764432No Hit
CCACATGGGATTGAGTACAGAGCTCACATCACAAACATTTAACCATTTTCGT1680.10178857059764432No Hit
GCTTTTTTGGTTTCCACATTTAAGTGAGAATGTGCAATAGTTGTCTTTCAGG1680.10178857059764432No Hit
TAGTTAGGGCATACAACTACAGAAATTTTCACATATCTTCTTTTCCCAAAAA1680.10178857059764432No Hit
GCAAACGGGAGTAACCATACATGAATAATCTAAAATAAAATAATGACAAACG1680.10178857059764432No Hit
GAGGCAGGAGAATCGCTTGAATCCGGGAGGTGGAGGTTGCAGTGAGCCAAAA1670.10118268624884881No Hit
ATTGTTGGGCAGGATGGGGAGTCGCTCCAGATGTTCCAGGCCCTGAAAAGAC1660.10057680190005332No Hit
GTTGTCGGGGGGAATGATTTTGAATATACAGCAAAAATTCGGACCCTAGCTG1660.10057680190005332No Hit
TAACTTGGGCTGAACCTTGAATTTAATCATGTTGGATTAATCTGGGGTTCAC1660.10057680190005332No Hit
TACTTAGGGCAAATATGATTCGGTGCTATAAAATGTATGTATAAAAGGTTAA1660.10057680190005332No Hit
AATAACGGGATTAAGGTATATTCCATCTATACCTAATTTGTTGAGAGTTGTT1660.10057680190005332No Hit
AAGCTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1660.10057680190005332No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)