FastQCFastQC Report
Sat 31 Dec 2022
SRR5844759.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844759.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147359
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGGACTCATCTCGTATGCCGTCT9350.6345048487028278No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCAGGACTCATCTCGTATGC7560.5130327974538372No Hit
GTGATGGGTGGCTCTACAGGAAGAGAAGCACCAGCTTTTCCTGCAGCTCAAG2190.14861664370686556No Hit
GCAGCTGGGATTCTGCTGATTCTTTATCTTGAGTAGGTGAAACTGTTATCTT2110.14318772521529055No Hit
GGTCATGGGGGGGACTCCTTGGGAATCTGTCACCTTCACGGCCCTGGGCACT2060.13979465115805617No Hit
TAAACCGGGCATTGTAAATATTGGTATTTTGAATGTTAAATGTCAAACAAAT2030.13775880672371554No Hit
CTATTTGGGATTGGAAGATTTACAAAAGAAAAAAAATGACCTTCGGTATATT1990.13504434747792807No Hit
GGTACTGGCTAGGACAGTTAACTTTTCTTCCTCGCTTAAGAAATTGCTTTAG1960.1330085030435874No Hit
TCTTAGGGGAAGATCACAATCACCAATGACCAGAATCGCCTGACACCTGAAG1940.13165127342069366No Hit
GTTGCCGGGGACACGTGGTCTTGGAGACGCAACAAGCAGGGCCGCGGACCAG1910.12961542898635306No Hit
TTTTATGGGATGGCTTCCTAAATGTCACCTGCCCATGCTGCTGCAGCTGAGC1880.12757958455201243No Hit
GGTGTAGGGGAAGGAGGCAGAAGGGAGAGTGATGCACGATGGGCAAGGACTT1870.12690096974056556No Hit
AATTATAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1860.12622235492911868No Hit
AAACGACGGGTATCGGAAAGAGAGCCATTTCTTAGGGGAATAAAACAGTTTC1850.1255437401176718No Hit
GCAAGCTGGGCTCCCCCCTTACTCCCCAGTGTTCAAATCCTGGATTCACTGC1840.12486512530622493No Hit
TGTCTTGGGAGGCACAGCTCCTCAGTCTTCTCACACAGTCCTGCCGGTGGCC1830.12418651049477805No Hit
CACGACGGGAGCTTAAAATGGTTTGTTCTATCTCAGGAATATTGTAAAAAAC1820.12350789568333119No Hit
GATGCTGGGCAGAAGGTAAAGCAGAATGAACATGTGAGGAATCTAAAAAACA1780.12079343643754369No Hit
ACTCGGGGGAGACAGATGCGAGGCGGCGGTCAGCAGGTGCCGAACCCACGGC1780.12079343643754369No Hit
AATAATGGGAGTTGACTGCTGAGAAAAGGACAATGAAATGATAGCAATTTCC1770.12011482162609681No Hit
CATCAGGGGGATTGAACCTAGTAAGATAGTCTGCCCCTTTGTCCTAGTACAT1750.11875759200320306No Hit
CGTTAGGGGGTTTGATGTAGTCCCAATTGTTTATTTTTGTTTCTGTTGCTCA1720.11672174756886244No Hit
CGCCATGGGAGAATTATAACAATCTGCTGTGTCAACGGAATCTAAATTAGCA1710.11604313275741557No Hit
CAGTAGTGGGTCCCTGGTGTGATTCCGTCCTGCGCGGTTGTTCTCTGGAGCA1710.11604313275741557No Hit
TTACTGGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1700.11536451794596869No Hit
GCAGCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1700.11536451794596869No Hit
TTGCACGGGGATGCTAGGGGCTGGGGCTCTGGTGTAGACCATGGACCAAAAG1700.11536451794596869No Hit
CGATCAGGAGACTGCGACAATGATAACAAACTAGGCATTGGACATATTTTTA1690.11468590313452182No Hit
TTCACGGGGAAGGCTCCAAGTGTCATCAGTCGTGTCCTTAAGAAAAACAATC1690.11468590313452182No Hit
TAAGCAGGGCAACTGGCAATACAAAGGAAAATATGGTACAGGAGTTAGTTAG1690.11468590313452182No Hit
CGTGTGGGGCAAGCCTCCTCCTGCTATTTCCTTTCTCTTTGGAGAACTTCCT1690.11468590313452182No Hit
ATAAGCGGGCGATCTGCATTTGGGCCTAACACATGAGGACTCATATAAACTT1680.11400728832307494No Hit
GCTGCCGGGGTTGATCTCATATTCATTTTTAGTTCTGAGAAAAACTCATAGT1660.11265005870018119No Hit
ACAAAGGGGAGGCTTAAGGAAATAAAATAGAATTAAGTGCAAAAAATAAAAC1650.11197144388873431No Hit
GGGTTAGGGACAGGAATGAATCCAGTGAGAACTCACTCATTACTGCAAGGTA1650.11197144388873431No Hit
CCAGAAGGGAGCTGAAATCTGTAATTCAATCACTTTTTCTCTTTTATCCTCT1640.11129282907728744No Hit
ATAACCGGGGGTTGCCCATTTTTATGGTTACTTCTTTGTAATATGCTAAACA1620.1099355994543937No Hit
TTTTTAGGGGTACTTCCCAATCAAATCATGTGATCATACAGTTATTCCCATG1610.10925698464294682No Hit
ACTAATGGGGAAAAAATTGCATCAAGAAGTCAGAATATAATTGAAATCAACA1610.10925698464294682No Hit
TTGCTAGGGAAACTTCTTAACTGGCAACAGTTGGAACAGTAATCAGTTTGCT1600.10857836983149995No Hit
AATTCGGGAAGAGCAAGGAGTTCTTGCAGATCAACAGCCTGGGGAAACTGCC1600.10857836983149995No Hit
ATTTGTGGGTAGCACTGCTGTACTGAATTGCATTAGGACACAGAGCATTCTG1590.10789975502005307No Hit
GTTACAGGGAACCATGAGGAAATTAGACAGTATGTTCCTTAATGACCAATGG1590.10789975502005307No Hit
ACGCACGGGGGAGATCATCGAGGCTATCTACAAAATGGTAGGCACTGTGATC1580.1072211402086062No Hit
GAGTATGGGATTTGACCCTGATCTCAAAGCATTACTATTCTTATTAAGAATT1570.10654252539715932No Hit
TCATAAGGGGGACATAGTCTCGTGGTGCGCCGTTTTTTAAGCCGGTCTGAAA1560.10586391058571244No Hit
ACATTAGGGGCATCTCCTCGACGATCAAGATCTATCTCTCTTCGTAGATCAA1560.10586391058571244No Hit
AGTATAGGGGACAAGCAGTTGGATGAAAGAGAACACACAATTGAAGAATGGA1560.10586391058571244No Hit
CGATATGGGAGCAGCTGACAGGTCTGATCCCCAGTGACCACGCCCCTCTGGC1560.10586391058571244No Hit
TGCTCTGGGTGAGTTTTCAACATTGCAAAATCAGTTTTTTACCTCTTTCCTA1550.10518529577426557No Hit
CTTCACCGGGCAGACTTTAAGTCAAAAACTGTAAAAAGAGACAAGGTCATTA1550.10518529577426557No Hit
TTGCACGGGAGTTCTTTATATATTTTAGATATAACTCCCTTATCAGATGTAT1550.10518529577426557No Hit
ATACGTGGGCAATATAATCCAGGGTCATTCTATTTTGTGCTAGAAATTAGTG1550.10518529577426557No Hit
CTCCAACAGGGGAACCTGCACTTCTCTAAAAAATAAAATTAGTTAAAATTAA1550.10518529577426557No Hit
CGAGATGGGGCCAGTTTCCTCATAAGTAAATTTAAATACTGTCTATTTCTAA1550.10518529577426557No Hit
CTTTGGGGGTTGTCAACCAGGACTCCACAAGGAAGTACAATTAGCATTACCT1540.10450668096281869No Hit
GTTATAGGGTTGAGAAAGTCACCTTGACAGTTCTTTGCACACAAGTTTTACA1530.10382806615137183No Hit
GCCATGGGTGAATCATTTTCTTCATACTTCTTACTCCACATTTCTTAAGTTT1520.10314945133992495No Hit
CCAACCTCCTCCAGTGAAGATACCGGGATGCTACCTTAATGGCACCTCTAGA1510.10247083652847808No Hit
AGCGCATGGGTAGCCAGAGCAATCAGACAAGAGAAAGAAATCAAGGGCATCC1510.10247083652847808No Hit
TTACTAGGGTCCCCACTTTCCAGCCGTTCCAGAGGAGCATATCTGCTGATGA1510.10247083652847808No Hit
TATAAAGGGTGAGCGCGCGAGGTGAGGAGTTGTGCAGGGTATGGGGAAAGGA1500.1017922217170312No Hit
TCATTTGGGCTGTTTGAACCAGTAAGCCACTTCTTTGACATTAGAAGACATT1500.1017922217170312No Hit
AGCCATGGGTTAATAATCCTGCCAACTCTCGTAGTGGAACTAATATGGGAGA1500.1017922217170312No Hit
CGAACAGGGGCAACCTCAGTAGAATCAATGTCATTCAAAGCAACCTCAGTTT1490.10111360690558432No Hit
TCAATGGGAAGCTTGCATTTGATTTTTGCATATAAGTAATGTCATAAGTGAA1490.10111360690558432No Hit
AATTGAGGGATTTCTGTATTCTCTATTCTGTTCTATTGGTCTGTGTGTCTCT1490.10111360690558432No Hit
AATCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG1480.10043499209413745No Hit
ATCGTAGGGGCCTCCCAAATTGCTGGAATTACAGGCATGAGCCACTGTGCCC1480.10043499209413745No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)