FastQCFastQC Report
Sat 31 Dec 2022
SRR5844758.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844758.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145709
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGGACTCATCTCGTATGCCGTCT9410.6458077400846893No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCAGGACTCATCTCGTATGC7100.48727257753467523No Hit
GGTACTGGCTAGGACAGTTAACTTTTCTTCCTCGCTTAAGAAATTGCTTTAG2020.13863247980564No Hit
GTGATGGGTGGCTCTACAGGAAGAGAAGCACCAGCTTTTCCTGCAGCTCAAG2020.13863247980564No Hit
CGTTAGGGGGTTTGATGTAGTCCCAATTGTTTATTTTTGTTTCTGTTGCTCA1980.13588728218572632No Hit
TCTTAGGGGAAGATCACAATCACCAATGACCAGAATCGCCTGACACCTGAAG1960.13451468337576952No Hit
CATCAGGGGGATTGAACCTAGTAAGATAGTCTGCCCCTTTGTCCTAGTACAT1840.12627909051602854No Hit
AGTATAGGGGACAAGCAGTTGGATGAAAGAGAACACACAATTGAAGAATGGA1820.1249064917060717No Hit
TAAACCGGGCATTGTAAATATTGGTATTTTGAATGTTAAATGTCAAACAAAT1810.12422019230109327No Hit
ATAAGCGGGCGATCTGCATTTGGGCCTAACACATGAGGACTCATATAAACTT1800.12353389289611486No Hit
TTTTATGGGATGGCTTCCTAAATGTCACCTGCCCATGCTGCTGCAGCTGAGC1770.1214749946811796No Hit
TGAGCAGGGATTTCTTTGTATTTCTCTGTTAAGCAAAGAAATATTAAGGAAC1760.12078869527620119No Hit
GCAAGCTGGGCTCCCCCCTTACTCCCCAGTGTTCAAATCCTGGATTCACTGC1740.11941609646624436No Hit
GCAGCTGGGATTCTGCTGATTCTTTATCTTGAGTAGGTGAAACTGTTATCTT1740.11941609646624436No Hit
CTATTTGGGATTGGAAGATTTACAAAAGAAAAAAAATGACCTTCGGTATATT1740.11941609646624436No Hit
GAATATGGGATTACATATCTTCTCTGACCACAGTGGAACAAAACTGGAAATA1730.11872979706126593No Hit
GAGTATGGGATTTGACCCTGATCTCAAAGCATTACTATTCTTATTAAGAATT1680.11529830003637388No Hit
TGTCTTGGGAGGCACAGCTCCTCAGTCTTCTCACACAGTCCTGCCGGTGGCC1670.11461200063139546No Hit
TAGTTAGGGCACTGGCTTTGCACAGAAGTATGCTCTACTTGCGTTGCTTTAG1630.11186680301148179No Hit
GATGGTGGGGGATGGAAGGACAATATCTCACTATCTCACCTTTAGAGATGTT1620.11118050360650338No Hit
TAAAGCGGGCTGCTGGTTCTGCTGGCCGGTTACAACCATGACAAGCTCATTC1620.11118050360650338No Hit
CGATCAGGAGACTGCGACAATGATAACAAACTAGGCATTGGACATATTTTTA1610.11049420420152496No Hit
GGTGTAGGGGAAGGAGGCAGAAGGGAGAGTGATGCACGATGGGCAAGGACTT1610.11049420420152496No Hit
CAGTAGTGGGTCCCTGGTGTGATTCCGTCCTGCGCGGTTGTTCTCTGGAGCA1610.11049420420152496No Hit
ACAAGTAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1610.11049420420152496No Hit
CTCTGCCGGGAAGGAAAATCACTTTATAAAATAAAACCAAGACATGACTCTG1600.10980790479654655No Hit
GCACTAGGGGATGGATGAAATTTTGGCTATTCTTGTTAACAGTGTCTCAGTG1600.10980790479654655No Hit
CGATATGGGAGCAGCTGACAGGTCTGATCCCCAGTGACCACGCCCCTCTGGC1600.10980790479654655No Hit
TACAAAAGGGTCTCAAACCCGGTATGGTGGTCACCTTTGCTCCAGTCAACGT1590.10912160539156812No Hit
ACATTAGGGGCATCTCCTCGACGATCAAGATCTATCTCTCTTCGTAGATCAA1590.10912160539156812No Hit
GCCATGGGTGAATCATTTTCTTCATACTTCTTACTCCACATTTCTTAAGTTT1590.10912160539156812No Hit
TTACTGGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1580.10843530598658971No Hit
CTTCACCGGGCAGACTTTAAGTCAAAAACTGTAAAAAGAGACAAGGTCATTA1580.10843530598658971No Hit
GATGCTGGGCAGAAGGTAAAGCAGAATGAACATGTGAGGAATCTAAAAAACA1580.10843530598658971No Hit
CGTGTGGGGCAAGCCTCCTCCTGCTATTTCCTTTCTCTTTGGAGAACTTCCT1580.10843530598658971No Hit
ATAACCGGGGGTTGCCCATTTTTATGGTTACTTCTTTGTAATATGCTAAACA1570.1077490065816113No Hit
AAACGACGGGTATCGGAAAGAGAGCCATTTCTTAGGGGAATAAAACAGTTTC1560.10706270717663288No Hit
CGAACAGGGGCAACCTCAGTAGAATCAATGTCATTCAAAGCAACCTCAGTTT1550.10637640777165445No Hit
GTCGCAGGGTAGAAGGGGAGAAGTAGAACCACAGTGTTTTATTTATTTTTCT1540.10569010836667604No Hit
GCAGCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1540.10569010836667604No Hit
ACGCACGGGGGAGATCATCGAGGCTATCTACAAAATGGTAGGCACTGTGATC1540.10569010836667604No Hit
GTTGCCGGGGACACGTGGTCTTGGAGACGCAACAAGCAGGGCCGCGGACCAG1540.10569010836667604No Hit
GTTACAGGGAACCATGAGGAAATTAGACAGTATGTTCCTTAATGACCAATGG1530.10500380896169763No Hit
TATAAAGGGTGAGCGCGCGAGGTGAGGAGTTGTGCAGGGTATGGGGAAAGGA1530.10500380896169763No Hit
CGTTCTGGGGTAATTGTTAATTTCTAGAAGCTTGCATCATAATGATTTTTTA1530.10500380896169763No Hit
TCAATGGGAAGCTTGCATTTGATTTTTGCATATAAGTAATGTCATAAGTGAA1520.10431750955671922No Hit
CACATAGGGGGGGGTTTCAGTGGCTTCTGGTGCTCTAGGGTGAGCTCTGCCC1520.10431750955671922No Hit
TGCTGTGGGACCTACACCCACCTGTTTTCCAGTGTTCTTCTCTCTAAACAGC1520.10431750955671922No Hit
ACTCGGGGGAGACAGATGCGAGGCGGCGGTCAGCAGGTGCCGAACCCACGGC1520.10431750955671922No Hit
TCTGCTGAGGGGGCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCT1520.10431750955671922No Hit
ACAAAGGGGAGGCTTAAGGAAATAAAATAGAATTAAGTGCAAAAAATAAAAC1510.10363121015174079No Hit
ATTTGTGGGTAGCACTGCTGTACTGAATTGCATTAGGACACAGAGCATTCTG1510.10363121015174079No Hit
TGGATTGGGAAGGCCACTCCAAGACCACACTGGCAGTTGTGTGGTGAACAAT1510.10363121015174079No Hit
ATGAGGGGCTGCAGAGAAATTAGACCAAATGAAACCTGCTGGAATAGGTTTG1510.10363121015174079No Hit
GAGCAGGGGGAGAATTGAAATTTATCTTTCAGTTTGCAAAGTTCCTGTACCC1510.10363121015174079No Hit
TTGAAAGGGGGAGAGGAGGTGTGGGTTAGGAACCAAGAGGTAGCCCTGGGGG1500.10294491074676237No Hit
AATTCGGGAAGAGCAAGGAGTTCTTGCAGATCAACAGCCTGGGGAAACTGCC1500.10294491074676237No Hit
GTCCCTAATCACAGATCCTAAGGTAATATAATATAATTTTAGTGCATTTCTC1500.10294491074676237No Hit
TCATAAGGGGGACATAGTCTCGTGGTGCGCCGTTTTTTAAGCCGGTCTGAAA1490.10225861134178396No Hit
GGGTTAGGGACAGGAATGAATCCAGTGAGAACTCACTCATTACTGCAAGGTA1490.10225861134178396No Hit
ATACGTGGGCAATATAATCCAGGGTCATTCTATTTTGTGCTAGAAATTAGTG1490.10225861134178396No Hit
AATTATAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1490.10225861134178396No Hit
TGGTTAGGGAAGTTTGAAGTTGGGAAGATGAAGTGAAACGATACTTGTTGCA1490.10225861134178396No Hit
TCGCCGAGGAAGATATCCTCATCTGGGACAAAAGCCTGGAGGTTCAGATTTC1480.10157231193680555No Hit
TGCTCTGGGTGAGTTTTCAACATTGCAAAATCAGTTTTTTACCTCTTTCCTA1480.10157231193680555No Hit
CTTTGGGGGTTGTCAACCAGGACTCCACAAGGAAGTACAATTAGCATTACCT1480.10157231193680555No Hit
GCTGCCGGGGTTGATCTCATATTCATTTTTAGTTCTGAGAAAAACTCATAGT1480.10157231193680555No Hit
TTTATCGGGTTCCTTACAACTATTTTATTCTAAGGACAGCTTTCCTTTCTGA1480.10157231193680555No Hit
TTGCTAGGGAAACTTCTTAACTGGCAACAGTTGGAACAGTAATCAGTTTGCT1470.10088601253182712No Hit
CATGTTGGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAAC1470.10088601253182712No Hit
TACTGCGGGATCTGAAAGCTACCATCTTGAAAATTTTTGAGGAAGTGTTTCT1460.10019971312684871No Hit
TTGCACGGGAGTTCTTTATATATTTTAGATATAACTCCCTTATCAGATGTAT1460.10019971312684871No Hit
CGCCATGGGAGAATTATAACAATCTGCTGTGTCAACGGAATCTAAATTAGCA1460.10019971312684871No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)