FastQCFastQC Report
Sat 31 Dec 2022
SRR5844751.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844751.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143472
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCAGGACTCATCTCGTATGC7020.48929407828705257No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGGACTCATCTCGTATGCCGTCT6610.4607170737147318No Hit
CCAAAGGGGAAAGCAGAAATAGACAAATCCACAACTGTAGTTGGAGACTCCA2090.14567302330768375No Hit
GCAAGTGGGATCTCTTTGGACAATCAACCAGCTAAAGACCAGGAAAATTTTT2040.14218802275008366No Hit
ACTTTTGGGATCCTGGATGATTCGCCAAAGGAAATAGCAAAGTTTATCTTCT1910.13312702130032342No Hit
CCTTCTAGGTAAGGATGGAGCATTTGATCATACTGGTTTCATTATATTCCTC1870.13033902085424334No Hit
GCGCCAGGCTCTTGGGAGTTCTCTTTATGACTTAGCATTTTCTTCTACTTGA1850.1289450206312033No Hit
GTTTCAGGGAACTTTATATTATTCCTCAGAAGCATTAGTTAAAAGTCTACTA1820.12685402029664325No Hit
TAAAATGGGGTCAATCACAATTTCACCCTCAGGACTAGGAAAGCACTTATCA1810.12615702018512323No Hit
CGTGAGGGGCCAGCTGCACAAAGCTGATTTTTTCCAAAGTCTAAAGACTGAG1770.12336901973904317No Hit
CTTACAGGGGGAAATACGAAATTGCAAATCTAAGTGAAAAACCTTTGTGTAA1770.12336901973904317No Hit
AATCCAGGGGACTGCTGACTTGGAGTTTCCAGCAGGGTGGTGTGCACCACTG1770.12336901973904317No Hit
CGTTTGGGGCTTGCTCGGGGATTGCAGTGCTTGTGTTCAAATTTTTCTTATT1770.12336901973904317No Hit
GTCTCCGGGGGCATTTAGGGGCGGTTGGCTTTGTTGGGTGAGCTTGTTTGTG1760.12267201962752315No Hit
ACATCAGGGCTGAAATTCAGTCCTGCCAATGCTCTGAGAGTGGGAAACCCTC1760.12267201962752315No Hit
ACATAAGGGAACCTAATTCATTGAAGTAATAACCAAATAATTTTCAATCTTG1740.12127801940448311No Hit
CAAGAAGAGGGAACAGCAGATTATGTAAATAATTGGCAGAAAAATTCTACTT1730.1205810192929631No Hit
TATAAAGGGAACCTCATTACTTGGTTTTTGTTTGTTTGTTTTTGAGACCCTG1730.1205810192929631No Hit
CTCCACGGGACCACGGTGTATCAAAAGTAGAAATGATAAATATATCTCATTT1710.11918701906992306No Hit
AAGCAAGGGAGGACAGGTTGAAATTGGGTCTTCAAAACCAGATATGGAAAAA1700.11849001895840304No Hit
GTAAAAGGGCGGCTGTTTTGTGTCATCTGTCTCCCTTCTTTTCCCCCCTCTG1700.11849001895840304No Hit
TTTATTAGGGGAGAGAGCAAGCCACATCAGTGCTCCTGGCAGATCCCTGCTT1690.11779301884688302No Hit
CAACATGGGATGACCTTTCAGGATGTCGTCAAGTGATGCTTTCAGTTGTAAC1690.11779301884688302No Hit
TCACATGGGGTTCTCTAGAAGAGCCTGGAGATCAGGAAGACTGAACTGCTGC1690.11779301884688302No Hit
ATCGTAGGGATGTTTTGTCTCGCTATACTGCACTTACGCTATCCAGTTGCAG1680.117096018735363No Hit
GTAAACGGGTACTTCCCACTCCTGACGAGTTCCCTCGCTTTGAAGGAGGGCG1680.117096018735363No Hit
GTAGCAGGGATGGGTACATATAAAATACTCCCAAATATCAAATTTTAATCTA1680.117096018735363No Hit
TTTTGCGGGGCACATCCACATCTAGAAAGGGCTGCTCACTGCACCAGAAAGA1660.11570201851232297No Hit
ATTGTAGGGACTTAAGCAAATTTACAAGAAAAAAACAACTCCATCAAAAAGT1660.11570201851232297No Hit
CAGTGCGGGATGTGGCACGTGGCTGGTCCTGTCTCTTTACTCCTGGCTCTTC1660.11570201851232297No Hit
ACAGGAGGGTTTTTCTAATATCTTAACATATCCTCCCCTTTAGGAGGAAGAA1650.11500501840080295No Hit
TGGGAGTGGGAAACCAAGAAGGAGAATGAAGAAGACAAACTCACTGACTGGA1650.11500501840080295No Hit
TATCTAGGGGATGTAAGACATGAAACTATAACACGACTGCAAGAAACATAGG1640.11430801828928293No Hit
AAAAATGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGG1640.11430801828928293No Hit
AAATTTGGGATAAAAAGTCAAACATTGTGATCTATAAATGCACAGAAAATAC1640.11430801828928293No Hit
CTCACGACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG1640.11430801828928293No Hit
TCTGGTGGGACACCTAGAAAGGAACTGCAATAATATCAAAACAAGTACTGGT1640.11430801828928293No Hit
ATACGAGGGCTGGGCATTCTGTCAAAGCACTGGTGGATTGTACCTAACAAGT1640.11430801828928293No Hit
TGCTTTGGGTAAATTAGTCAGTTATTTTATTTTCCCACTGTGCTCTTTGAAA1640.11430801828928293No Hit
ATTTAAAGAGCTTCTGCATGGGAAAAGAAACTATCAACAGAGTAAACAGACA1630.11361101817776291No Hit
GCTACCGGGATTTTATTGAGGATTTTTGCATCAATGTTCATCAAGGATATTG1630.11361101817776291No Hit
TAGGCAGGAATTGGAGGAAAAGACTGAGCAGTTGTTGGATTGTAAACAAGAA1630.11361101817776291No Hit
CCAAATGGGGTACTATAAAAATAAAAATCGTGTTCATAAGAAAATGTGAAAA1620.11291401806624289No Hit
TTTAAAGGGTAACCTCAACAAAGCACCTAAAAACAGTATTTTCTTATGTAAG1620.11291401806624289No Hit
ACGTATGGGCATCCCCAGCAGCCCTGCTTCTTCACAGTTCTCTGTCTGTAGT1610.11221701795472287No Hit
GATTTTGGGATGGTGTCTGAATGATTGTTTTCTCTCTTTGTATATTTGTACA1610.11221701795472287No Hit
CGTTTTGGGGTTTTGCACAACCAAGGCCAACTTAAATAGGAATATTCATAGT1610.11221701795472287No Hit
AATGTAGGGGACAAAATGTTGAACAGAGTCCAACTTTCTGTTTAAGCCTTTA1610.11221701795472287No Hit
GAATTGGGGAGTGTCGTCTGATGCATATTCAGCCTCCCAAGAAGAAGAATAA1600.11152001784320285No Hit
CTCGCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1600.11152001784320285No Hit
ACTGCAGGGACTTCTTGAAATCCTTAGGGCATCTTTGGACAAGGAAATCAGA1600.11152001784320285No Hit
GATAACGGGGCGAATCGGATCAACGAAAGTCTGCGTTGGGATGGAATTCTTG1600.11152001784320285No Hit
GTACGAGGGAAGGATTTTCTCACTTATCATGATGTAAACTTTGAAAAAGTTT1590.11082301773168284No Hit
TACCTTGGGAACATTCTGTGACCAATAATATTTTAGTTAACAGAGATTCTGG1590.11082301773168284No Hit
TTGAGAGGGGTTTAAATAATTCCTACAACCTTATTTTAAAATGCAAGCAATT1580.11012601762016282No Hit
GCCTGCCTCAGCCTCCCAAGGTGCTGGGATTACAGGTGTGAGCCACCTCGCC1580.11012601762016282No Hit
GAAGTTGGGAGCACTCTTCCCTCTCAGTTTTCTTTTCATCCCAGCCATGTTT1580.11012601762016282No Hit
TCTTGCGGGAGATGTGGATCAGCAAGCAGGAGTATGACGAGTCCGGCCCCTC1580.11012601762016282No Hit
TTAGGAACGGGTTACTACTCCGGAAAAAAAGAACCATTTGGATACATAGGTA1560.10873201739712278No Hit
TACAATGGGGGATAAATGAAGAAATAAAAATGTTTGTCTAGAATGTAGCATC1560.10873201739712278No Hit
CATGTAGGGATCTAAATAAAGTTCATCTTGTTTGTTTTCTATTATTTCTTTT1550.10803501728560276No Hit
TGTGGTGGGAATGGCTTTTTATTTTATTAACAGACAATGGGAAAACTAGCTC1550.10803501728560276No Hit
TCCGTTGGGGGCATAAGAAAGAGGATTAATTGTTACATTTCCACTTTTCTGG1540.10733801717408276No Hit
TGTTGAGGGGTTACAGATCTCATGGACATTAAAAGGATAATCAAGGGATATT1530.10664101706256274No Hit
GGCTCTGGGAACTGAAAATGTTTTCTTGGTGTGACACATGTATCTTTGTAAT1520.10594401695104272No Hit
GTCAACGGCACCACAGGGATGGATGTCCTCTGGGCAGGGACCCCCATGGTGA1520.10594401695104272No Hit
ACGAGGGGGATTGTTTTTGTATTGGTATTTAAACATCTGAATATGTAACATC1510.1052470168395227No Hit
AACTGAGGGCTATTTTGACTTTCTTAGTTACCATGTGTCTTCTCACAAAAAC1500.10455001672800268No Hit
AACCTAGGGATTGCAATAACTTTCCAAATTTTCTAAATCCATGAATACCCTT1500.10455001672800268No Hit
CTCATGGGGGGAGCACTAAGGGGTGGGTGGGGAAGTGAAATGTTAGGCGATG1500.10455001672800268No Hit
AGTCTTGGGTTAGGAAATAACTCAGGACAAAGGGCCAAGATGAATGCAACCT1500.10455001672800268No Hit
ATACAAGGGCCTGCTAGTTGGCGACCTCATGGCTGTGAACAACTGCGGGCTG1490.10385301661648266No Hit
TGCCCGGGGAGCTAAGAATTGTGAAAGCTTAAAATCTGTGTTTTCTTTTTCT1490.10385301661648266No Hit
ATAAAGGGAGGGAAAGCAGGTCCACTGTGGCCTGTGAGGACGAGAGCTCTGG1490.10385301661648266No Hit
TGAAGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1490.10385301661648266No Hit
CATACAGGGGAGAAAGTGTCATCTTGGTTTATTTTTGCCTTGGGACTTCCAA1490.10385301661648266No Hit
CACGGGGGTACGGTATGTGCTATAAATCTTTCAAGTCTTCAGAAATATGATC1490.10385301661648266No Hit
CGCCTAGGGCTCAAGGGTAGGAAGAATCAATATTGTGAAAATGGCCATACTG1480.10315601650496264No Hit
TCGAAAGGGAAACAGAGTGAGGGCTAAAATTCACATCTCCTGACAGTCAGCT1480.10315601650496264No Hit
CAAAAAAAGAAAGAAAAGAAAAATGACCTGTATCTGTAAAATATCAATATAT1480.10315601650496264No Hit
GGATTAGGGAACTTTTTGTTTTCAGCAACATAAATTGATTTTTAGCTGGCAG1470.10245901639344263No Hit
ACTAGCGGGGACGCTCTTCCGATCTACACCCGGGAGTGTTCAGGGATGGTGT1460.10176201628192261No Hit
GCTTATGGGGAATGTTCTAAAGAAAATTTATGAAGATGGAGACGATGATATG1460.10176201628192261No Hit
GTGAATGGGCTAGTTCTCAGAGCATTTGGAAAGGTTATCTTAAATGGCTACC1460.10176201628192261No Hit
AATGCAGGGGTCTTAAAAGAATGGGACATGTCATGAACTGGTTTCTTTTTTT1460.10176201628192261No Hit
TTCTATGGGATTGGCCTCTTGGGACTGACTGTAACGTTTTGGTGTTTGTATG1450.10106501617040259No Hit
TTCACAGGGAGTCCTAAAGTGTACTTCATGAAGCAGACCATTGGGAATTCCT1450.10106501617040259No Hit
CTTTAAGGGCTTGTGGTTTTGATTTGCATTTCTCTAATGATCAGTGATGTTG1450.10106501617040259No Hit
TCCGTTGGGATATAATTCGGTATGCCATAATTGATAATAAAGAAGATGTACA1450.10106501617040259No Hit
ATTATAGGGAATTCCAGTTTTTTTCCGATGAGAAAAGTTTGACCCCAGAACC1450.10106501617040259No Hit
CCTTCAGGGATCACTCTAATAGATCAAAGGAAAAAAACTTAGAATCATTTTC1440.10036801605888257No Hit
CGTACTGGGGCAGGAAACACCTCTTCACATTTAAGGTTTTCTACTTGGGTGT1440.10036801605888257No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)