FastQCFastQC Report
Sat 31 Dec 2022
SRR5844750.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844750.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143549
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCAGGACTCATCTCGTATGC7310.5092337807995876No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGGACTCATCTCGTATGCCGTCT6770.4716159638868958No Hit
CCTTCTAGGTAAGGATGGAGCATTTGATCATACTGGTTTCATTATATTCCTC2260.15743753004200656No Hit
ATTGTAGGGACTTAAGCAAATTTACAAGAAAAAAACAACTCCATCAAAAAGT2060.1435050052595281No Hit
AATCCAGGGGACTGCTGACTTGGAGTTTCCAGCAGGGTGGTGTGCACCACTG2000.13932524782478456No Hit
GTCTCCGGGGGCATTTAGGGGCGGTTGGCTTTGTTGGGTGAGCTTGTTTGTG1970.1372353691074128No Hit
CCTTCAGGGATCACTCTAATAGATCAAAGGAAAAAAACTTAGAATCATTTTC1950.13584211662916496No Hit
CGTGAGGGGCCAGCTGCACAAAGCTGATTTTTTCCAAAGTCTAAAGACTGAG1940.13514549039004103No Hit
GGCTCTGGGAACTGAAAATGTTTTCTTGGTGTGACACATGTATCTTTGTAAT1910.13305561167266927No Hit
ACGAGGGGGATTGTTTTTGTATTGGTATTTAAACATCTGAATATGTAACATC1900.13235898543354532No Hit
GCAAGTGGGATCTCTTTGGACAATCAACCAGCTAAAGACCAGGAAAATTTTT1890.13166235919442143No Hit
CCAAAGGGGAAAGCAGAAATAGACAAATCCACAACTGTAGTTGGAGACTCCA1890.13166235919442143No Hit
TATAAAGGGAACCTCATTACTTGGTTTTTGTTTGTTTGTTTTTGAGACCCTG1870.13026910671617356No Hit
ACATAAGGGAACCTAATTCATTGAAGTAATAACCAAATAATTTTCAATCTTG1870.13026910671617356No Hit
GTAAAAGGGCGGCTGTTTTGTGTCATCTGTCTCCCTTCTTTTCCCCCCTCTG1850.12887585423792572No Hit
CGTTTGGGGCTTGCTCGGGGATTGCAGTGCTTGTGTTCAAATTTTTCTTATT1850.12887585423792572No Hit
ACATCAGGGCTGAAATTCAGTCCTGCCAATGCTCTGAGAGTGGGAAACCCTC1850.12887585423792572No Hit
GTAGCAGGGATGGGTACATATAAAATACTCCCAAATATCAAATTTTAATCTA1840.1281792279988018No Hit
CTTACTGGGGGATATTCACATGGAACTGCCCTATGGGACCCTGACAGATGAT1830.12748260175967788No Hit
ATACAAGGGCCTGCTAGTTGGCGACCTCATGGCTGTGAACAACTGCGGGCTG1820.12678597552055396No Hit
TAAAATGGGGTCAATCACAATTTCACCCTCAGGACTAGGAAAGCACTTATCA1820.12678597552055396No Hit
CTTACAGGGGGAAATACGAAATTGCAAATCTAAGTGAAAAACCTTTGTGTAA1800.1253927230423061No Hit
TTTAAAGGGTAACCTCAACAAAGCACCTAAAAACAGTATTTTCTTATGTAAG1800.1253927230423061No Hit
CATGTAGGGATCTAAATAAAGTTCATCTTGTTTGTTTTCTATTATTTCTTTT1770.12330284432493434No Hit
CAGTGCGGGATGTGGCACGTGGCTGGTCCTGTCTCTTTACTCCTGGCTCTTC1760.12260621808581043No Hit
TGTTGAGGGGTTACAGATCTCATGGACATTAAAAGGATAATCAAGGGATATT1760.12260621808581043No Hit
CTCCACGGGCTGGCAATGACGACTATGTTGAGCTTGCATTCAATGCACGGAA1760.12260621808581043No Hit
CCAAATGGGGTACTATAAAAATAAAAATCGTGTTCATAAGAAAATGTGAAAA1750.1219095918466865No Hit
TTAGGAACGGGTTACTACTCCGGAAAAAAAGAACCATTTGGATACATAGGTA1740.12121296560756259No Hit
TTCACAGGGAGTCCTAAAGTGTACTTCATGAAGCAGACCATTGGGAATTCCT1740.12121296560756259No Hit
ATACGAGGGCTGGGCATTCTGTCAAAGCACTGGTGGATTGTACCTAACAAGT1740.12121296560756259No Hit
TTTTGCGGGGCACATCCACATCTAGAAAGGGCTGCTCACTGCACCAGAAAGA1720.11981971312931473No Hit
GATAACGGGGCGAATCGGATCAACGAAAGTCTGCGTTGGGATGGAATTCTTG1720.11981971312931473No Hit
GCTTATGGGGAATGTTCTAAAGAAAATTTATGAAGATGGAGACGATGATATG1710.1191230868901908No Hit
GCGCCAGGCTCTTGGGAGTTCTCTTTATGACTTAGCATTTTCTTCTACTTGA1710.1191230868901908No Hit
AATGCAGGGGTCTTAAAAGAATGGGACATGTCATGAACTGGTTTCTTTTTTT1700.11842646065106689No Hit
GTGCAGCAGGGATCGCTGGGTCAATAGTACTTGCCGCAGCACTCTTAAAACT1690.11772983441194296No Hit
TGCTTTGGGTAAATTAGTCAGTTATTTTATTTTCCCACTGTGCTCTTTGAAA1690.11772983441194296No Hit
CGTTTTGGGGTTTTGCACAACCAAGGCCAACTTAAATAGGAATATTCATAGT1680.11703320817281905No Hit
CAAGAAGAGGGAACAGCAGATTATGTAAATAATTGGCAGAAAAATTCTACTT1670.11633658193369512No Hit
TGCCCGGGGAGCTAAGAATTGTGAAAGCTTAAAATCTGTGTTTTCTTTTTCT1670.11633658193369512No Hit
ACTTTTGGGATCCTGGATGATTCGCCAAAGGAAATAGCAAAGTTTATCTTCT1660.1156399556945712No Hit
CATACAGGGTCCTCCTAGGCCTCTGGGCCTGTGATTGGAGGGGCTGCCATGA1650.11494332945544726No Hit
CAAAAAAAGAAAGAAAAGAAAAATGACCTGTATCTGTAAAATATCAATATAT1650.11494332945544726No Hit
ATTTAAAGAGCTTCTGCATGGGAAAAGAAACTATCAACAGAGTAAACAGACA1640.11424670321632335No Hit
TGGGAGTGGGAAACCAAGAAGGAGAATGAAGAAGACAAACTCACTGACTGGA1640.11424670321632335No Hit
AAGCACGGGGAATATCATGAAAGGCTTCAGACCTTTTTGATGTGGTTTATTG1630.11355007697719942No Hit
AACTGAGGGCTATTTTGACTTTCTTAGTTACCATGTGTCTTCTCACAAAAAC1630.11355007697719942No Hit
TCCGTTGGGATATAATTCGGTATGCCATAATTGATAATAAAGAAGATGTACA1630.11355007697719942No Hit
GTAAACGGGTACTTCCCACTCCTGACGAGTTCCCTCGCTTTGAAGGAGGGCG1620.11285345073807551No Hit
ACTAGTGGGAATTATTGACTATGGATTTTAGGCTGGGTAAGGAGGAAAATAA1620.11285345073807551No Hit
GTCAACGGCACCACAGGGATGGATGTCCTCTGGGCAGGGACCCCCATGGTGA1620.11285345073807551No Hit
CTGTACGGGACGATCGCAGAGTTGTATTTCCTCACAAACTTCTTTACGAAGA1620.11285345073807551No Hit
AAGCAAGGGAGGACAGGTTGAAATTGGGTCTTCAAAACCAGATATGGAAAAA1610.11215682449895158No Hit
GAAGTTGGGAGCACTCTTCCCTCTCAGTTTTCTTTTCATCCCAGCCATGTTT1610.11215682449895158No Hit
CTTACCCGGGAGGAGTCCCTGGAACACAGGTCATGACTTAAGTAATGGGAGT1610.11215682449895158No Hit
GTACGAGGGAAGGATTTTCTCACTTATCATGATGTAAACTTTGAAAAAGTTT1610.11215682449895158No Hit
GTGCCCGGGATGACTTAGTTGCGTTACACCCTTTCTTGACAAAACCTAACTT1610.11215682449895158No Hit
TATACAGGGATGAACAAAATGGAAATACCAGTGGAGAGAGAGAGAGTAAACC1610.11215682449895158No Hit
ACTAGCGGGGACGCTCTTCCGATCTACACCCGGGAGTGTTCAGGGATGGTGT1600.11146019825982766No Hit
CTCACGACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG1600.11146019825982766No Hit
GGAATAGGGCTGCCAAAAAAGTAGGGGGTGGGAGTGGCATAACCAAGTGTCT1590.11076357202070372No Hit
CGGCTAGGGGTAGCTGAGGTATGTGCTTCCCAGGCCTCCAAAGCTTCCACAT1580.11006694578157981No Hit
AACCTAGGGATTGCAATAACTTTCCAAATTTTCTAAATCCATGAATACCCTT1580.11006694578157981No Hit
GACATAGGGCAGAAAATTCATCCCACATCAGTTATTAGTGGCTATCGACTTG1580.11006694578157981No Hit
TAGGCAGGAATTGGAGGAAAAGACTGAGCAGTTGTTGGATTGTAAACAAGAA1580.11006694578157981No Hit
ACTCGGGGGTTTCTTGCAGTTAATGTAAGAATACTTTAAATCTCTAAGCTTC1560.10867369330333197No Hit
AAAAATGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGG1560.10867369330333197No Hit
AAATTTGGGATAAAAAGTCAAACATTGTGATCTATAAATGCACAGAAAATAC1560.10867369330333197No Hit
TCGCTCGGGTGCCATGTAGAACTATGTTCCATAAATATTGGCTGGAATTTAG1560.10867369330333197No Hit
TACAATGGGGGATAAATGAAGAAATAAAAATGTTTGTCTAGAATGTAGCATC1560.10867369330333197No Hit
TGTGGTGGGAATGGCTTTTTATTTTATTAACAGACAATGGGAAAACTAGCTC1560.10867369330333197No Hit
CAGCTTGGGTCGACCCGGTTCTGATAGAGTAACAGGTTCCCAGTAATTAAGG1550.10797706706420804No Hit
CAACAAGGGTTAGCAAAGAAAAAAAATAGAAGATCCAAATAAGTACAGTCAG1550.10797706706420804No Hit
GGGAAAGGGAGTTCATGGCCCCGTGATCCAGAGTCCCTGCTGGCGCCTTCGA1550.10797706706420804No Hit
ACAAAGGGGACCTAGGGAAAATCCTATCAGATCACTCTGAATTCAAAGCAAG1540.10728044082508412No Hit
TCAATAGGGCGTTGGACATATTTTAAATCGCTGAATATTTTCATTGGTCCGG1540.10728044082508412No Hit
ATCGTGGGGGGGAAGAGCTTAGAAACTGGAAATACTAGATTTGGGAGAAGGG1540.10728044082508412No Hit
CTTGTTGGGCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCGGAGCTTGAGGA1540.10728044082508412No Hit
ACTGCAGGGACTTCTTGAAATCCTTAGGGCATCTTTGGACAAGGAAATCAGA1540.10728044082508412No Hit
GGATTAGGGAACTTTTTGTTTTCAGCAACATAAATTGATTTTTAGCTGGCAG1530.1065838145859602No Hit
CAACATGGGATGACCTTTCAGGATGTCGTCAAGTGATGCTTTCAGTTGTAAC1530.1065838145859602No Hit
TCAAGCCTTCAGATGCCCAGCTGAAATGCGTCTTATCTTGTTAAATGAGAAT1530.1065838145859602No Hit
GAAAAAGGGCCAGGTTCAAGCGATTCTCCTGCCTCAGCTTCAGTAGCTGGGA1520.10588718834683628No Hit
TTCTATGGGATTGGCCTCTTGGGACTGACTGTAACGTTTTGGTGTTTGTATG1510.10519056210771234No Hit
CTAAAAGGGCAGCACACAGACTTTCTCTAGAGTGTACAGCTTCTTCCCACAG1510.10519056210771234No Hit
TCTAGTGGGCGATCTTAAGGTTGGGGTGCTTATAAAATGAAAAAGGAAATTG1510.10519056210771234No Hit
CTGTTGGGGGGACTGGATTCTGAAGGCCACTTCCCACCATCATAGCTGCCAT1500.10449393586858843No Hit
GCTACCGGGATTTTATTGAGGATTTTTGCATCAATGTTCATCAAGGATATTG1500.10449393586858843No Hit
ATACGGGGGTACTTTTTTCTCCCCAGTTCTAAGCGAAGAACACAATTCTAAG1490.1037973096294645No Hit
GAACAAGGGAAGCCACAGATTGGGAGAAAATGTTTGCCAAGAATACACATCT1490.1037973096294645No Hit
AAACAAGGGCGATCTAGATTTGGGGACACCTGTATAAGAAAGTACCATCCCC1490.1037973096294645No Hit
GATTTTGGGATGGTGTCTGAATGATTGTTTTCTCTCTTTGTATATTTGTACA1490.1037973096294645No Hit
AATGTAGGGGACAAAATGTTGAACAGAGTCCAACTTTCTGTTTAAGCCTTTA1490.1037973096294645No Hit
GGCTTATGGGATCCTCTAGAATAATTACATATTTCAAAAATCATATCCCATA1480.10310068339034059No Hit
TGCTAGGGGCATCTTGTGCCAGATGATCCATCTGTAGCCCTCTCAGCTAGAG1480.10310068339034059No Hit
CTTTAAGGGCTTGTGGTTTTGATTTGCATTTCTCTAATGATCAGTGATGTTG1480.10310068339034059No Hit
CTCCACGGGACCACGGTGTATCAAAAGTAGAAATGATAAATATATCTCATTT1470.10240405715121666No Hit
ATCGTAGGGATGTTTTGTCTCGCTATACTGCACTTACGCTATCCAGTTGCAG1470.10240405715121666No Hit
TGAAGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1460.10170743091209274No Hit
CGTTTAGGGTCTGCACTTGAAATGCCCTTCCTTCTGACTTGATCCTCCAAGG1460.10170743091209274No Hit
TGTGAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1450.1010108046729688No Hit
ATAAAGGGAGGGAAAGCAGGTCCACTGTGGCCTGTGAGGACGAGAGCTCTGG1450.1010108046729688No Hit
CGTGGAGGGAATGTGGTGGTATCCATCCCTTCGAGCATTTATCCTTTGTGTT1450.1010108046729688No Hit
TCTGGTGGGACACCTAGAAAGGAACTGCAATAATATCAAAACAAGTACTGGT1450.1010108046729688No Hit
TATAGTGGGGGATGCCGATGGCCTCATAACTCACACTAGTACCTCACCTCAG1450.1010108046729688No Hit
TAGCTTGGGGTTTGTCTAATCTTTAATGCCATCTGTAAACTCTACATCTTTT1450.1010108046729688No Hit
TCACATGGGGTTCTCTAGAAGAGCCTGGAGATCAGGAAGACTGAACTGCTGC1450.1010108046729688No Hit
GAATTAGGGAAGTGCAAAGTAAAACCTCCTTGCTCTAGTCTCTTGGGAGAGT1450.1010108046729688No Hit
GCGCATGGGCTTTGCCTCCACACCCAGATTCCTGGAAATGGCTAACGTTTCC1440.1003141784338449No Hit
TAACGTGGGGTACTTATACTTTGAAACAAATGTATGTTATTGTTGTTGGGCT1440.1003141784338449No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)