FastQCFastQC Report
Sat 31 Dec 2022
SRR5844749.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844749.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142501
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCAGGACTCATCTCGTATGC7050.4947333702921383No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGGACTCATCTCGTATGCCGTCT6850.4806983810639926No Hit
GCAAGTGGGATCTCTTTGGACAATCAACCAGCTAAAGACCAGGAAAATTTTT2030.14245514066567952No Hit
CGTTTGGGGCTTGCTCGGGGATTGCAGTGCTTGTGTTCAAATTTTTCTTATT2030.14245514066567952No Hit
TGCTTTGGGTAAATTAGTCAGTTATTTTATTTTCCCACTGTGCTCTTTGAAA1970.1382446438972358No Hit
ACTGCAGGGACTTCTTGAAATCCTTAGGGCATCTTTGGACAAGGAAATCAGA1900.13333239766738478No Hit
CCAAAGGGGAAAGCAGAAATAGACAAATCCACAACTGTAGTTGGAGACTCCA1900.13333239766738478No Hit
CTTACAGGGGGAAATACGAAATTGCAAATCTAAGTGAAAAACCTTTGTGTAA1890.1326306482059775No Hit
CTCCACGGGACCACGGTGTATCAAAAGTAGAAATGATAAATATATCTCATTT1860.13052539982175562No Hit
GTAGCAGGGATGGGTACATATAAAATACTCCCAAATATCAAATTTTAATCTA1850.12982365036034835No Hit
ATACGAGGGCTGGGCATTCTGTCAAAGCACTGGTGGATTGTACCTAACAAGT1810.1270166525147192No Hit
TGTGGTGGGAATGGCTTTTTATTTTATTAACAGACAATGGGAAAACTAGCTC1780.12491140413049732No Hit
GTTTCAGGGAACTTTATATTATTCCTCAGAAGCATTAGTTAAAAGTCTACTA1760.12350790520768275No Hit
ATTGTAGGGACTTAAGCAAATTTACAAGAAAAAAACAACTCCATCAAAAAGT1760.12350790520768275No Hit
CCTTCTAGGTAAGGATGGAGCATTTGATCATACTGGTTTCATTATATTCCTC1750.12280615574627547No Hit
GCGCCAGGCTCTTGGGAGTTCTCTTTATGACTTAGCATTTTCTTCTACTTGA1740.12210440628486817No Hit
TCTGGTGGGACACCTAGAAAGGAACTGCAATAATATCAAAACAAGTACTGGT1740.12210440628486817No Hit
TTTTGCGGGGCACATCCACATCTAGAAAGGGCTGCTCACTGCACCAGAAAGA1720.1207009073620536No Hit
TACAATGGGGGATAAATGAAGAAATAAAAATGTTTGTCTAGAATGTAGCATC1720.1207009073620536No Hit
GTCAACGGCACCACAGGGATGGATGTCCTCTGGGCAGGGACCCCCATGGTGA1720.1207009073620536No Hit
CCAAATGGGGTACTATAAAAATAAAAATCGTGTTCATAAGAAAATGTGAAAA1710.1199991579006463No Hit
GTGCCCGGGATGACTTAGTTGCGTTACACCCTTTCTTGACAAAACCTAACTT1710.1199991579006463No Hit
GGCTCTGGGAACTGAAAATGTTTTCTTGGTGTGACACATGTATCTTTGTAAT1670.11719216005501715No Hit
TGCTAAGGGATGTGTACAATTAGAGGGTCCTGACTTGATGATAACAAATTTA1670.11719216005501715No Hit
CGTTTAGGGTCTGCACTTGAAATGCCCTTCCTTCTGACTTGATCCTCCAAGG1670.11719216005501715No Hit
ATACAAGGGCCTGCTAGTTGGCGACCTCATGGCTGTGAACAACTGCGGGCTG1660.11649041059360987No Hit
GCTACCGGGATTTTATTGAGGATTTTTGCATCAATGTTCATCAAGGATATTG1650.11578866113220258No Hit
GATAACGGGGCGAATCGGATCAACGAAAGTCTGCGTTGGGATGGAATTCTTG1650.11578866113220258No Hit
CAGTGCGGGATGTGGCACGTGGCTGGTCCTGTCTCTTTACTCCTGGCTCTTC1640.1150869116707953No Hit
TGCCCGGGGAGCTAAGAATTGTGAAAGCTTAAAATCTGTGTTTTCTTTTTCT1630.114385162209388No Hit
ACTTTTGGGATCCTGGATGATTCGCCAAAGGAAATAGCAAAGTTTATCTTCT1630.114385162209388No Hit
ACGAGGGGGATTGTTTTTGTATTGGTATTTAAACATCTGAATATGTAACATC1620.1136834127479807No Hit
ATCGTAGGGATGTTTTGTCTCGCTATACTGCACTTACGCTATCCAGTTGCAG1620.1136834127479807No Hit
ACAGGAGGGTTTTTCTAATATCTTAACATATCCTCCCCTTTAGGAGGAAGAA1620.1136834127479807No Hit
ATCACAGGGATATTTTGCCTATTTTTTAAACTGGGTTGTTTATCTTATTACT1610.11298166328657343No Hit
TAACGTGGGGTACTTATACTTTGAAACAAATGTATGTTATTGTTGTTGGGCT1600.11227991382516615No Hit
TTCACAGGGAGTCCTAAAGTGTACTTCATGAAGCAGACCATTGGGAATTCCT1600.11227991382516615No Hit
GTCTCCGGGGGCATTTAGGGGCGGTTGGCTTTGTTGGGTGAGCTTGTTTGTG1600.11227991382516615No Hit
CTCGCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1590.11157816436375885No Hit
GCATGTGGGCACCTGACACATCAGGACTCACATAAACTTAAGGTAAAGGGGC1590.11157816436375885No Hit
TATAAAGGGAACCTCATTACTTGGTTTTTGTTTGTTTGTTTTTGAGACCCTG1580.11087641490235156No Hit
TGCTGGGTCTCTAGCAGGGGCTAGGAAAAATAAGTATAATAAGTTAAGACAT1580.11087641490235156No Hit
TTTAAAGGGATTGTTGAATCTTGTATGATCAAGTCACTCCTCTGCTCAGAAA1570.11017466544094427No Hit
ATTTAAAGAGCTTCTGCATGGGAAAAGAAACTATCAACAGAGTAAACAGACA1570.11017466544094427No Hit
AATCCAGGGGACTGCTGACTTGGAGTTTCCAGCAGGGTGGTGTGCACCACTG1570.11017466544094427No Hit
CGTTTTGGGGTTTTGCACAACCAAGGCCAACTTAAATAGGAATATTCATAGT1570.11017466544094427No Hit
TTCTATGGGATTGGCCTCTTGGGACTGACTGTAACGTTTTGGTGTTTGTATG1560.10947291597953698No Hit
AGAAATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1560.10947291597953698No Hit
TCCGTTGGGATATAATTCGGTATGCCATAATTGATAATAAAGAAGATGTACA1560.10947291597953698No Hit
AAGCAAGGGAGGACAGGTTGAAATTGGGTCTTCAAAACCAGATATGGAAAAA1550.10877116651812968No Hit
GAACAAGGGAAGCCACAGATTGGGAGAAAATGTTTGCCAAGAATACACATCT1550.10877116651812968No Hit
ACATAAGGGAACCTAATTCATTGAAGTAATAACCAAATAATTTTCAATCTTG1550.10877116651812968No Hit
TGTAGTGGGGGAGTGGATGAAAATGGGTAAAAATAAAATGAAAAAATGAGTC1540.1080694170567224No Hit
GCTTATGGGGAATGTTCTAAAGAAAATTTATGAAGATGGAGACGATGATATG1540.1080694170567224No Hit
ATACAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1540.1080694170567224No Hit
TTAACGCGGGGAAACATACAAAAACTTCTTAAAACCCAATAAGAAAACAATC1540.1080694170567224No Hit
ACATCAGGGCTGAAATTCAGTCCTGCCAATGCTCTGAGAGTGGGAAACCCTC1540.1080694170567224No Hit
TAGGCAGGAATTGGAGGAAAAGACTGAGCAGTTGTTGGATTGTAAACAAGAA1540.1080694170567224No Hit
TGTTGAGGGGTTACAGATCTCATGGACATTAAAAGGATAATCAAGGGATATT1530.10736766759531513No Hit
AATGTAGGGGACAAAATGTTGAACAGAGTCCAACTTTCTGTTTAAGCCTTTA1530.10736766759531513No Hit
TATCTAGGGGATGTAAGACATGAAACTATAACACGACTGCAAGAAACATAGG1520.10666591813390783No Hit
TCCGTTGGGGGCATAAGAAAGAGGATTAATTGTTACATTTCCACTTTTCTGG1520.10666591813390783No Hit
GCCTGCCTCAGCCTCCCAAGGTGCTGGGATTACAGGTGTGAGCCACCTCGCC1520.10666591813390783No Hit
TAACGTGGGTCTCTGTTCTATGATTATATATCTTTGAGATATCAACATAGCT1520.10666591813390783No Hit
CGTGAGGGGCCAGCTGCACAAAGCTGATTTTTTCCAAAGTCTAAAGACTGAG1520.10666591813390783No Hit
TCTTGCGGGAGATGTGGATCAGCAAGCAGGAGTATGACGAGTCCGGCCCCTC1520.10666591813390783No Hit
ACTCGGGGGTTTCTTGCAGTTAATGTAAGAATACTTTAAATCTCTAAGCTTC1510.10596416867250055No Hit
GACAGGGGGCTCAAATGTCAGCTAGAAGCAACTTTTTCAGTTTTCTATCTAA1510.10596416867250055No Hit
TTGAGAGGGGTTTAAATAATTCCTACAACCTTATTTTAAAATGCAAGCAATT1500.10526241921109325No Hit
TCAAGAGGGCTGGCTAGGCATTAGAGGCTAGGAACCAAGGACTAAGACAAGG1500.10526241921109325No Hit
TGTCAGGGGCTCCTTATTCTGGTTTGGGTTGTTATTATTATTTTTCTGTATA1490.10456066974968596No Hit
ACGTATGGGCATCCCCAGCAGCCCTGCTTCTTCACAGTTCTCTGTCTGTAGT1490.10456066974968596No Hit
GTAAAAGGGCGGCTGTTTTGTGTCATCTGTCTCCCTTCTTTTCCCCCCTCTG1490.10456066974968596No Hit
ACATAGGGGGGAAATACAGAGAACGCCACAAAGATACTCCTCAAGAAGAGCA1490.10456066974968596No Hit
TAAAATGGGGTCAATCACAATTTCACCCTCAGGACTAGGAAAGCACTTATCA1490.10456066974968596No Hit
CTCCACGGGCTGGCAATGACGACTATGTTGAGCTTGCATTCAATGCACGGAA1490.10456066974968596No Hit
CGGCTAGGGGTAGCTGAGGTATGTGCTTCCCAGGCCTCCAAAGCTTCCACAT1470.10315717082687138No Hit
TTTAAAGGGTAACCTCAACAAAGCACCTAAAAACAGTATTTTCTTATGTAAG1470.10315717082687138No Hit
CGTGGAGGGAATGTGGTGGTATCCATCCCTTCGAGCATTTATCCTTTGTGTT1460.10245542136546411No Hit
GAAGTTGGGAGCACTCTTCCCTCTCAGTTTTCTTTTCATCCCAGCCATGTTT1460.10245542136546411No Hit
GGAATAGGGCTGCCAAAAAAGTAGGGGGTGGGAGTGGCATAACCAAGTGTCT1460.10245542136546411No Hit
GAAAAAGGGCCAGGTTCAAGCGATTCTCCTGCCTCAGCTTCAGTAGCTGGGA1450.1017536719040568No Hit
ATACGGGGGTACTTTTTTCTCCCCAGTTCTAAGCGAAGAACACAATTCTAAG1450.1017536719040568No Hit
AACCTAGGGATTGCAATAACTTTCCAAATTTTCTAAATCCATGAATACCCTT1450.1017536719040568No Hit
GTAAACGGGTACTTCCCACTCCTGACGAGTTCCCTCGCTTTGAAGGAGGGCG1450.1017536719040568No Hit
TCTAGTGGGCGATCTTAAGGTTGGGGTGCTTATAAAATGAAAAAGGAAATTG1450.1017536719040568No Hit
AACTGAGGGCTATTTTGACTTTCTTAGTTACCATGTGTCTTCTCACAAAAAC1440.10105192244264953No Hit
TTGTAGGGGACGGGCAATCTACTTTACTGAAAGCCCAGCAATTTAAATGTTA1440.10105192244264953No Hit
TACGTGGGGAAGCCGAAGTACTCTTGGAGTAGTAGTACTTGCAGGAGAACTT1440.10105192244264953No Hit
TTTATTAGGGGAGAGAGCAAGCCACATCAGTGCTCCTGGCAGATCCCTGCTT1430.10035017298124224No Hit
CATACAGGGTCCTCCTAGGCCTCTGGGCCTGTGATTGGAGGGGCTGCCATGA1430.10035017298124224No Hit
CAAGAAGAGGGAACAGCAGATTATGTAAATAATTGGCAGAAAAATTCTACTT1430.10035017298124224No Hit
TCGAAAGGGAAACAGAGTGAGGGCTAAAATTCACATCTCCTGACAGTCAGCT1430.10035017298124224No Hit
ATCACGGGGAAGGCAGTGATTTGAAAGTTTACATTTTTCATGATTTTCAAAT1430.10035017298124224No Hit
CTCTGCGGGCCTGAAGATCAGTGTACTGTTACCCGGACATACCTATTCCTTC1430.10035017298124224No Hit
TCAAGCCTTCAGATGCCCAGCTGAAATGCGTCTTATCTTGTTAAATGAGAAT1430.10035017298124224No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)