FastQCFastQC Report
Sat 31 Dec 2022
SRR5844739.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844739.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62522
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCAGGACTCATCTCGTATGC6080.9724576948913982No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGGACTCATCTCGTATGCCGTCT6070.9708582578932217No Hit
CACAGCGGGAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGT1480.236716675730143No Hit
CTAGTAGGGAGCTGGTGATTGCTCTGGTGAGACTGAATGGACTTGCTGGTGA1430.2287194907392598No Hit
TCAAGCGGGCACTTCTCCCACCTGCAGAGCCAGTGTCCTTGGGTGGGCTAGA1360.2175234317520233No Hit
TTCTAAGGGAGAGAAGGAATGCTTTCAGTGGGAAAGAACACCATCATCATTA1360.2175234317520233No Hit
CATGATGGGATCAAGGAATGTTGACCGCATTCCTTTGAATTCACAACTAAGT1360.2175234317520233No Hit
AGAGAGGGGTGTGTCAAATGTGTATTTGACAATTGTTGAATTGTCAAACATG1350.21592399475384663No Hit
GTCGCGGGGGTGAATTAGAGCTGAAAGTCTTGCAAAGTGTGGACTCTCTCTA1330.21272512075749336No Hit
CTATCAGGGGACGGGACACCTGCATCTGCCAACAAGACTGGAAGCAGGTGAG1320.21112568375931673No Hit
AGCACCGGGGACAGAAATTATGCAATGGTTTGTTGAGATACGGACTTGATGG1320.21112568375931673No Hit
GTCACGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1280.20472793576661016No Hit
TAAATTGGGCACACATCTGACATTCAAAGTCAGAAATTCACGTGGTCTTGCA1280.20472793576661016No Hit
GTAATTGGGGTGTGTATGTATATTTAACTTTTTAATTTGATTTTATTTTTAA1270.20312849876843353No Hit
ATCTCGGGGATATTTGTAATTACGCACAATGTTTACACACTGATATTGATGA1260.20152906177025687No Hit
GTAGGCGGGACATTTTCTCCTGTGCGAATTTCAAAAAGCTATATTCATTTTG1260.20152906177025687No Hit
CCGGGATGGGCAAACTTAGAAAGTCTAATATCTTAATGTTTACTTATGTAGC1250.19992962477208023No Hit
TAGCCTGGGACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT1250.19992962477208023No Hit
AGCCAAGGGGGGCTCTCCCCTTCTCAGGGGCTTGTTGGCTTGCCTCTGATTT1230.19673075077572696No Hit
TTAGCCGGGAAGCTAAGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCT1220.1951313137775503No Hit
TAAACGGGGTCTTACAATTACAAAGCCGATGCTGCTGAGAAAATCAGAAAAG1220.1951313137775503No Hit
CACGTAGGGTACACCAATGGATAAACTAACGGAAACTGCTTTTTGACATTAA1210.19353187677937367No Hit
AAAATAGGACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAA1210.19353187677937367No Hit
TCAATGGGGTGACTCTAGTTAACAATTTATTGTATATTCTTAAATAACTAAA1190.19033300278302037No Hit
TTACTAGGGAACAAGTTGCAGAATTAAAACAGGAACTGGCAACTTTAAAGTC1190.19033300278302037No Hit
GGCTATGGGGAAATCTTTGATGGAATTAGCCATGCAGAACAGATACTAAATG1180.18873356578484374No Hit
TAGACAGGGATGAAAACAATATGTTTTAAAATTTTCATTGCACAATATCGTT1180.18873356578484374No Hit
ACACCAGGGACTTTGACCGCGATGATGTGGCTTTGAAGAACTTTGCCAAATA1160.18553469178849044No Hit
TGCGCAGGGAGCTTTGGCAAGGTCATGCTTTCAGAACGAAAAGGCACAGATG1160.18553469178849044No Hit
CATTTTGGGGATGAAATAGTCAAGGACCAGAATCTGTAATATTTTTATGTAA1160.18553469178849044No Hit
AACATAGGGGTTTTGAGTAGGGAAAGGTTCATTTATTTTCATTACCTGGCAT1160.18553469178849044No Hit
GTTTTTGGGAATGGATTCACAAAATATGATATCTTAAAACATGTTGAAAGAT1140.18233581779213717No Hit
CGTTACGGGGAGGTAATGATAGTTCCCATATCAAAGAGTGGTGAGGACTGAA1140.18233581779213717No Hit
GCGTTAGGGGTGCCCGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1130.1807363807939605No Hit
GTTTTAGGGTCCGATCTGCCATGGCCAGTCACCCGGAGCCTTCAGCTGGCAG1120.17913694379578388No Hit
TTAAAGGGGACTCCGGAAAAAAAGAACCATTTGGATACATAGGTATGGTCTG1120.17913694379578388No Hit
CCAGATGGGGGGAGGAAAAGAAAGAAATTAGAATCTCATAGAAATGAAATAC1100.1759380697994306No Hit
TATTAAGGGGAGGCGGCGGCAGGGCTGAGCCAGCGACGCCCTCCATTCACTC1100.1759380697994306No Hit
CGACGAGGGGAACAACTGATTGCATAAGAATATAGAACCACCTGGAAGGTGT1100.1759380697994306No Hit
CATAAAGGGCTTACTTGGAGAGACATATGTCTGAATTTATGGAGTGTAATTT1090.17433863280125397No Hit
ATGACTGGGCATGTCATCAAGTAGAGAAAATATTTTTATTTTTGCCCAATAT1090.17433863280125397No Hit
TTGATCAGGGACGCTCCTCATACTAGGCCTACTAACCAACACACTAACCATA1080.1727391958030773No Hit
AAAAAAGGGGGTGTCACCCACTGCATCTGGAGTACAGCTTCTCCTAGGACAG1040.16634144781037075No Hit
TGACTCGGGGCATTGGGGATTACATTTCAACATGAGATTTGGGCAGGGATAA1040.16634144781037075No Hit
GCTTGGGGGATGGCCCATCGATGAGCATTTTTAACATACTCCATAGTCTTTT1030.1647420108121941No Hit
TCAGTAGGGAGAGATAAGCAGGTACACTTGAAGTCAAAATGCAATCACAGTT1030.1647420108121941No Hit
TTGACAGGGAAATATATTAATTTTATGTTTGAGGCATATGATAAAATGAAAT1030.1647420108121941No Hit
TGAGTAAGGGGATAATTACGTTTAAAGTGCAGTCATTTGTGGTTAGAATCTT1020.16314257381401745No Hit
TAAAGAGGGAAGGTACTGTGATCACCAGCCTACTTCCAATCTCCACCCACCT1020.16314257381401745No Hit
TGGCCTGGGGTGTGGTTCTCAGTGATATTCACATCGACATCATGGACTATAT1010.16154313681584084No Hit
GTTATGGGGGGGACAGAGAGCATGAGCAGGCTCTGGTGTCTCCTCCTCTTCT1000.15994369981766418No Hit
CTAATGTCGGGAATCCGGTCACCTGCAATGTCTGGAGGAATTTTTGCTATAC990.15834426281948755No Hit
TTAGTGGGGGGATGGCAAGTTGGATCCCTCTACCTTCCGAGGTCAGGAGTGC990.15834426281948755No Hit
AAATTAGGGACTCTTAGCTGCTGTCACTAGGATTGGCCATCAGATTCTGGAT990.15834426281948755No Hit
TCTGCCGGGTATACCACTCATATCACAGTTTTTGAAATTCATCTTTCATTAT980.1567448258213109No Hit
GCGCAAGGGGGGCTGGGAAGAGAGGCGAAGCCAGGTCACCTTTCAAGGACCC970.15514538882313425No Hit
GACTCAGGGTAAATATTGAATGACAAAACTCAGATGGAGGAAAAAGAACAAA960.15354595182495762No Hit
ACACAGAGGTAGAATAGATGCCAAAGTCATTTATTCAGTCCTTAGTTTTCTT950.15194651482678098No Hit
GTCTTGGGGTGGATCTTGCACCAACATCTGGTACTTCAGCTAAGACACCCTC950.15194651482678098No Hit
GTTCCAGGGAACCTATTTTTAAAGAAACCTTTTTTGCTATTGACTGTTTTTT950.15194651482678098No Hit
GTCAATGGGTGAGCTACACATTATTTAGATATTTCCCCTAGAGATTAGAAAC950.15194651482678098No Hit
TGGTTTGGGGAGCCGTACGCTCTTTACAGATACTAATGTCAAGAGTTAAACC940.15034707782860432No Hit
GGTTAGGGGGAATGAGTAATGATCATGTTAGGGTTAGGGTATTTACAGTATC930.14874764083042769No Hit
ACATTTGGGTATCTGCATCTTTAGTTAGGCTGGTCTCAGGTTGCTCATAGGT930.14874764083042769No Hit
CCTCTAGGGGCTCTTCTGAGGATCCGGCAAGATGGCAGAAGTAGAGCAGAAG930.14874764083042769No Hit
GACCCAGGGAGTCTAGGGACTGAGGAGTCGCCGCCGCCCCGAGTCCCGGTAC920.14714820383225105No Hit
GTGCAAGGGTGCCCGCTTCGGCCTCCCATAGTGCTGGGATTACAGGCGTGAG920.14714820383225105No Hit
TGTGCTGGGTAGGGCTAATGAGTCATTGTTCCTTAATATCGTTTTCAATATG910.1455487668340744No Hit
GCTACCGGGGATATTTCAAATTAGTGAGAAGGAATGGTATATTTAATAAGCA900.14394932983589778No Hit
GCCAACGGGTACTTACTCACATTTTTGAAATGTATGAAGAAATAAATCAGTT890.14234989283772112No Hit
CTAAAACGGGATGACATTTATTTATTGTATCTGAATGATTTATTATGATATT890.14234989283772112No Hit
TCGAGCGGGAATATCACATAGTTGGCATTGCTGAATAGTTTCATCTCTTTGG890.14234989283772112No Hit
CTTGCTGGGCTCAGCCCATGGGGAGAAACGGAGGCCTGAATACAGGCGGTCA880.14075045583954449No Hit
GCTATAGGGTAAGCTGATAAGCAACTTCAGCAAAGTCTCAGGATACAAAATC880.14075045583954449No Hit
ATATATGGGCCGATCTTACTGAGGGATCTTTGCAAAAGTGTAGCATTAAAGC880.14075045583954449No Hit
CATGAGGGGGTTATTTTGCCGTTTGACATTTTTTATGGTTCATTTATTTTTA880.14075045583954449No Hit
GACCATGGGCCTGTAAAGCCCTTAATCCAAAAGCATTGTGCTCTTAATCTGT860.1375515818431912No Hit
ACTGCTGGGCAAACCTCTCAGGCATTATTAAAAGGATAATAAGGAAATACTA860.1375515818431912No Hit
AAAACTGGGAGTGAGTTGCAGAATGAAAATTGAGCTGGATTGGATGCATTGC860.1375515818431912No Hit
GATTCTGGGAGGACATATGAAGAGTGCAATGGCAAAACAAGAGCAAAAAGCA860.1375515818431912No Hit
TATGCCGGGGCTTTTGGATTTGGTATCTAGTAAAATTTACCCACAGTAGCCA840.13435270784683792No Hit
TATTAGGGGTCGCCCAGGCTGGAGTGCAGTGGTACGATCACAGCTCACTGCA840.13435270784683792No Hit
AGTTGCGGGCACTTTTTTGTATTTTTGCCATTTGAAAAGGTTGTGGTGTAGT840.13435270784683792No Hit
AATTTGGGGAAGGGAACTGAGAGTTTTAAGAATCAGAGGGTTATCAACAGTG830.13275327084866126No Hit
TGCTGCGGGTGACTCGCAACAACTTTCTTTAGAATAATTCCCGCATGAGTCC830.13275327084866126No Hit
AGGCTCGGGGAATTTCGCAAGCTGAAGCTTGTAATGGAATCCTAATAGAGAT820.13115383385048462No Hit
AAGACTGGGATCCATCACTGTGGGGATTGCTTAGTGTGTTAAAGCATGAAGC810.129554396852308No Hit
TTTGTGGGGAATGAACAATCAAGATGCCATAGAAAAGGCTGTTAGTAGAGGC810.129554396852308No Hit
CCAACTGGGAGGTCTCAATTTCTCACTGGAAAATGAGTCTTTAAAATAGTAA810.129554396852308No Hit
GTTCAGGGGCGATCTTTGCGTGGGTATACTATTATATAATGATTTTATGGTT800.12795495985413133No Hit
GTACTTGGGTAACTGTTCGTGCTTACTTAAATCAGACAGTTACAGAATTCAA800.12795495985413133No Hit
GATAGTGGGATAACTGGCTAGATAAAACACAACTCACTTAACCTTAACACTC800.12795495985413133No Hit
CTCTCGTTTCGGGAAGTGAGATACAAAAGTAATTGCAGGCCCAGGGCTGATT800.12795495985413133No Hit
AGTTCTGGGTAAGACCAGACCACAGAGGCAGAATGAAGCAATAGCTCCAGCA790.12635552285595472No Hit
GGGTAGGGGGAGGCTGCCAACCATTTTTCTGAAGTGATTATACTAATTTATA790.12635552285595472No Hit
TCCATTGTGTAATAAACTTAGTAAAGCACCTGTTGTACAACTCAATGGCATT790.12635552285595472No Hit
ATGCTGCGGGTGTTCGATTGCGGGTTCTCTGTCTCTGCGGAGGTGCCTTCCC790.12635552285595472No Hit
AAGTCAGGGTTCATCATCTTAAGTATTGTAAGCTGCTATGTATGGATTTAAA790.12635552285595472No Hit
AACTTGGGGCAGTGGCGTGATCTTAGCTCACTGCAAGCTCTGCCTTCCAGGT790.12635552285595472No Hit
TTTTATGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC790.12635552285595472No Hit
TAACCCGGGATGGTTCAATGTACTTACAATGGAATCCCCACTAAATTCTGTT780.12475608585777807No Hit
TACGTGGGATATGAGCTCCGGCAGGGACTGAGGCTGGCAGAGCTGGCTCTCT780.12475608585777807No Hit
CAGTTTGGGATCACCAGCTAGAGGTTATGGACTATACACACCTATTATTATA780.12475608585777807No Hit
TTAAATGGGAAAACAAATACACATTAAACAATAAGTGAAATAATTCCCAAGA760.12155721186142478No Hit
TTCTTTGGGGCTTTAGCCTGAGACTTGCCATAAGAAACTGCTGAGTACTTGG760.12155721186142478No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAGGACTC760.12155721186142478No Hit
CAACCTGGAGATCTCAGCCTCAGTTCTCTTGTCTGTAAAATGGAAGAACTGG760.12155721186142478No Hit
ATAGCAGGGGTTTTTATACCTTCCCGCGCGGACGCCGGCGCTGCCAACGGAA750.11995777486324814No Hit
TATTACGGGTACATTTTGGATTAAACCTGTTTCCCGGTTATGTGTAGGGAAC750.11995777486324814No Hit
TAAATAGGGGTGAGACAGACAATATTATCCTTATTGTACAGATGGGGGGGCT740.1183583378650715No Hit
AGCGAAGGGCTTTTAAAAATAATTTAAAACAACTGTCTTTAATATGCCTATG740.1183583378650715No Hit
GCATAGGGGTCCATAACTGCTATTTAGACAACAGAAAGCACTTCTTCATAAC740.1183583378650715No Hit
AGTTTGGGGTCAACACTGGCAGCCACCGTCTCACCCCTGCGGAGGAGCGCTC730.11675890086689485No Hit
ATTGATGGGGTGAGCGTGTGGGGAAAATTACCTCACTTTCCAGAACTTCCAT730.11675890086689485No Hit
GAACTTGGGATGTTCATGATAGGCTATTTTTGGAGCTCTTCTAAGATAGACA730.11675890086689485No Hit
ATTCGCGGGTGAACCCAAGTAGAGAAGATAGATCTTGGAAGGAGAGATCCAT730.11675890086689485No Hit
GTATCCGGGTATGAAATCTGTATAAGTGTGTCCTTATACAAATTTTAGGCCA720.11515946386871821No Hit
TGGTTAGGGCATGCATATCTTCTCCAACCATGAAATCAAAGCACGGTGCAGA720.11515946386871821No Hit
GGTAACGGGGCCCCAGATGTCAAGTAATGTTGGATTTTCTATAATAATTTAG720.11515946386871821No Hit
CGTTTGGGGAGGCGTGGGAAAGATGAATTACAAACAGTTCTTGAGATCAAGG720.11515946386871821No Hit
TTTAATGGGGGAGGAGCAGGCACATCTTACATGGCTGGAGAAGGAGGAAGAG720.11515946386871821No Hit
TTAACTGGGAGCCAGATCTTATCTATGAAATTTCCCTTTGTAGACACACACA710.11356002687054156No Hit
GTGCAGGGATAAGAGGAGATTTATTATGGGAATTGGCTTATGTGATTATGGA710.11356002687054156No Hit
GCGCTGGGGAACAACAGCCCGTGGAACAGTAAACCTCCCGCCACTCAAGAGA710.11356002687054156No Hit
ACCATTGGGGTAGGTAAATAACAGATGGCAAGAATGGGAGATGGGAATGGGG710.11356002687054156No Hit
ATGGATGGGAAGTGAAAAGAAAAAAATCAGTGTAAGTTAAAGGGCTTATATA710.11356002687054156No Hit
AATAAAGGGCACCAGCAGGTGAAGTGCATCATCACAGAGGAGAAGGTGTGCA700.11196058987236492No Hit
CAAGCTGGGGGGTCTGAAGACAGACAGCCAGGGTTCAAATCCGGATCCCACC700.11196058987236492No Hit
GTACGAGGGGCCTCTCGTCTCCTTCCTGGTTTGGGCGGAAGCCATCACCTGG700.11196058987236492No Hit
TTGAATGGGGCCTGTGCCTAACCTGTGCTGCTCTGTAGCAAATGGGCTTGCC700.11196058987236492No Hit
GCGGCTGGTGTCCTACCCCTTTGACACTGTTCGTCGTAGAATGATGATGCAG700.11196058987236492No Hit
GTAAAAGGAAGGAAAAACTAGCACCCTTCTGTCCACTCAGCAATAAGAGGGA700.11196058987236492No Hit
ATGTGGGTAGCTCATGGATTGAGGTAAGATTTAAGTAAAAAGCGCCTAGGTT690.1103611528741883No Hit
GATACAGGGCACGAAAATGGTACTGAAATGGCCACATAATGCAATGTGATTT690.1103611528741883No Hit
CTGAAGAGGGTAGGTACCAAATTGTGAAAGTTTGTTTTCAGTTATATTACTT690.1103611528741883No Hit
CTTAGTGGGGGAGGATTTTTGTCCACTGATCTTTTGCAACAGAAAAGCATAC690.1103611528741883No Hit
GTACTTGGGGGCTTTGCGGCATGGCGATGGCGGAGGGCGAGAGGACTGAGTG690.1103611528741883No Hit
ACTACTGGGTATCATGTGTCATTAGAGCCCACTATGCCATCCAAACAGCTCT680.10876171587601165No Hit
TGTCTAGGGAAGAGGTTTAATGGACTCACAGTTTCATGTGGCTGGGGAGTCT670.10716227887783501No Hit
ATGATAGGGATGATAAAGGGTCAATTCACCAAGAAGACATAACAATCCTGGA670.10716227887783501No Hit
TGGCCTGGGACAGCATGTTTATTTATATTACAAGCCATTCAGGGACAAAGAA670.10716227887783501No Hit
AAGGTAGGGGAGGGCAAGACCCCAGAGCCTTAGCTGAGCAAGCCCAGAGGGG670.10716227887783501No Hit
TAAGGGGGTGAGCACACCATTTGTGCTGCTGCTGTTGTCGTGAAATATAATA670.10716227887783501No Hit
CCGTCCGGGAAGGCACTGAATATGCTTCATACAGAACAATTATCTGACTACA670.10716227887783501No Hit
CACAAGGGGAACCTAAATTTTGCTTGGTATTTACTATTGCTTTTAAAATAGA670.10716227887783501No Hit
CAACCGGGGAATGGTGCATACAAGGCCATCCCCGTTGCCCAGGACCTGAACG670.10716227887783501No Hit
AAAGATGGGTGTTATAGGAAACATAAATAAGATTACAGAGGTATCAATTTGG660.10556284187965836No Hit
CAGATGGGGTTTGTGCGGGGAAAGGGGTGAGTCTATGCATACCAGGTACCAC660.10556284187965836No Hit
CACCAAGGGTCTGCATCAGGGTACTTTCATGTCTCTATATTCATTTTTCACA660.10556284187965836No Hit
CTTTTGGAGGGAAGTGTTTAAAATTATGCTCTGTAACTCTGTACTGCTAGTA660.10556284187965836No Hit
GTTAGGGGGTTCTGACTAAATGGCATTAAAAGAGGTCTGAGGAATTTGCATG660.10556284187965836No Hit
GTCCTGGGAGGGGTTTTTTCTAAGATCTCTTTGGGTTAAAATGATTTGTGAT660.10556284187965836No Hit
TGTTAAGGGTAATGAGATTTTACCGTGCAGCTAGTGTGTCCTTTACTCACCC660.10556284187965836No Hit
TACCACGGGAAGTCTGGAGATATCATCATTGTAGGCCGCTTTCAGCTTTCAT660.10556284187965836No Hit
CTTTAAGGGTCCGGACCGTTATTTTAGAAATTCCTGAACTCCTCAGTCCAGT660.10556284187965836No Hit
ATTGTCGGGAAGAAATCACCTGAAGGTACAAAACTCACTGGTAATAGTACTC660.10556284187965836No Hit
GTATATGGGCAGCTTGGGCATATGAGGAAGTGAGAAATAGGGTTTCTTTCTA660.10556284187965836No Hit
ATTACAGGGTATGACTTTTGCCTCATACCTTGAATCCTAAAAGTTTTCGCTG650.10396340488148172No Hit
ATAGCAGGGAATGATATGTCAGCATTAGCAGGCTGATCAACATTGTATGCTA640.10236396788330508No Hit
CTACCAGGGGGAAGACTTGCCATTTTGTTTGGACAAAGTTGCAATTTAAATG630.10076453088512843No Hit
AAGCATGGGCAGTTTAAAAGTATTTTTAGCTCGTATGACTTGTTTTCATTCA630.10076453088512843No Hit
AGTAAAGGGCAGCCACCAGGCTCCCTGTGTCCCTTCCATGGGAAGGTCTTCC630.10076453088512843No Hit
AGAAATGGGCACTTTAGGATTAATTCTCCATTAAACTTCCCATGTGATTTGC630.10076453088512843No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)