FastQCFastQC Report
Sat 31 Dec 2022
SRR5844737.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844737.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61654
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGGACTCATCTCGTATGCCGTCT6381.03480714957667No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCAGGACTCATCTCGTATGC5720.9277581341032212No Hit
CTAGTAGGGAGCTGGTGATTGCTCTGGTGAGACTGAATGGACTTGCTGGTGA1560.2530249456645149No Hit
GTTTTTGGGAATGGATTCACAAAATATGATATCTTAAAACATGTTGAAAGAT1510.24491517176501118No Hit
CACAGCGGGAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGT1500.24329321698511047No Hit
CCGGGATGGGCAAACTTAGAAAGTCTAATATCTTAATGTTTACTTATGTAGC1370.2222078048464009No Hit
TTCTAAGGGAGAGAAGGAATGCTTTCAGTGGGAAAGAACACCATCATCATTA1370.2222078048464009No Hit
AACATAGGGGTTTTGAGTAGGGAAAGGTTCATTTATTTTCATTACCTGGCAT1330.21571998572679796No Hit
CTATCAGGGGACGGGACACCTGCATCTGCCAACAAGACTGGAAGCAGGTGAG1310.21247607616699649No Hit
AGAGAGGGGTGTGTCAAATGTGTATTTGACAATTGTTGAATTGTCAAACATG1310.21247607616699649No Hit
GTCGCGGGGGTGAATTAGAGCTGAAAGTCTTGCAAAGTGTGGACTCTCTCTA1290.209232166607195No Hit
GTCACGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1270.20598825704739354No Hit
TAAACGGGGTCTTACAATTACAAAGCCGATGCTGCTGAGAAAATCAGAAAAG1260.20436630226749278No Hit
ATCTCGGGGATATTTGTAATTACGCACAATGTTTACACACTGATATTGATGA1230.1995004379277906No Hit
TGCGCAGGGAGCTTTGGCAAGGTCATGCTTTCAGAACGAAAAGGCACAGATG1220.19787848314788983No Hit
AGCCAAGGGGGGCTCTCCCCTTCTCAGGGGCTTGTTGGCTTGCCTCTGATTT1220.19787848314788983No Hit
AAAACTGGGAGTGAGTTGCAGAATGAAAATTGAGCTGGATTGGATGCATTGC1200.19463457358808836No Hit
GTAGGCGGGACATTTTCTCCTGTGCGAATTTCAAAAAGCTATATTCATTTTG1200.19463457358808836No Hit
TATGCCGGGGCTTTTGGATTTGGTATCTAGTAAAATTTACCCACAGTAGCCA1190.19301261880818762No Hit
TCAAGCGGGCACTTCTCCCACCTGCAGAGCCAGTGTCCTTGGGTGGGCTAGA1190.19301261880818762No Hit
TTAAAGGGGACTCCGGAAAAAAAGAACCATTTGGATACATAGGTATGGTCTG1190.19301261880818762No Hit
GTAATTGGGGTGTGTATGTATATTTAACTTTTTAATTTGATTTTATTTTTAA1190.19301261880818762No Hit
GGCTATGGGGAAATCTTTGATGGAATTAGCCATGCAGAACAGATACTAAATG1180.1913906640282869No Hit
TTACTAGGGAACAAGTTGCAGAATTAAAACAGGAACTGGCAACTTTAAAGTC1180.1913906640282869No Hit
TAGACAGGGATGAAAACAATATGTTTTAAAATTTTCATTGCACAATATCGTT1170.18976870924838615No Hit
AGCACCGGGGACAGAAATTATGCAATGGTTTGTTGAGATACGGACTTGATGG1160.18814675446848542No Hit
CATTTTGGGGATGAAATAGTCAAGGACCAGAATCTGTAATATTTTTATGTAA1150.18652479968858468No Hit
AAAAAAGGGGGTGTCACCCACTGCATCTGGAGTACAGCTTCTCCTAGGACAG1150.18652479968858468No Hit
CATGATGGGATCAAGGAATGTTGACCGCATTCCTTTGAATTCACAACTAAGT1140.18490284490868394No Hit
GATAGTGGGATAACTGGCTAGATAAAACACAACTCACTTAACCTTAACACTC1140.18490284490868394No Hit
TCTGCCGGGTATACCACTCATATCACAGTTTTTGAAATTCATCTTTCATTAT1120.18165893534888247No Hit
GTGCAAGGGTGCCCGCTTCGGCCTCCCATAGTGCTGGGATTACAGGCGTGAG1120.18165893534888247No Hit
TAAATTGGGCACACATCTGACATTCAAAGTCAGAAATTCACGTGGTCTTGCA1110.18003698056898174No Hit
TATTAAGGGGAGGCGGCGGCAGGGCTGAGCCAGCGACGCCCTCCATTCACTC1100.178415025789081No Hit
GCGTTAGGGGTGCCCGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1090.17679307100918026No Hit
CACGTAGGGTACACCAATGGATAAACTAACGGAAACTGCTTTTTGACATTAA1080.17517111622927953No Hit
TCCATTGTGTAATAAACTTAGTAAAGCACCTGTTGTACAACTCAATGGCATT1080.17517111622927953No Hit
AAATTAGGGACTCTTAGCTGCTGTCACTAGGATTGGCCATCAGATTCTGGAT1080.17517111622927953No Hit
TAAAGAGGGAAGGTACTGTGATCACCAGCCTACTTCCAATCTCCACCCACCT1070.1735491614493788No Hit
GCTTGGGGGATGGCCCATCGATGAGCATTTTTAACATACTCCATAGTCTTTT1070.1735491614493788No Hit
TCAGTAGGGAGAGATAAGCAGGTACACTTGAAGTCAAAATGCAATCACAGTT1070.1735491614493788No Hit
AAAATAGGACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAA1070.1735491614493788No Hit
TAGCCTGGGACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT1070.1735491614493788No Hit
TTTTATGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1070.1735491614493788No Hit
CCAGATGGGGGGAGGAAAAGAAAGAAATTAGAATCTCATAGAAATGAAATAC1060.17192720666947806No Hit
CGACGAGGGGAACAACTGATTGCATAAGAATATAGAACCACCTGGAAGGTGT1050.17030525188957732No Hit
CATAAAGGGCTTACTTGGAGAGACATATGTCTGAATTTATGGAGTGTAATTT1050.17030525188957732No Hit
TGGCCTGGGGTGTGGTTCTCAGTGATATTCACATCGACATCATGGACTATAT1050.17030525188957732No Hit
GCATAGGGGTCCATAACTGCTATTTAGACAACAGAAAGCACTTCTTCATAAC1050.17030525188957732No Hit
GCCAACGGGTACTTACTCACATTTTTGAAATGTATGAAGAAATAAATCAGTT1040.16868329710967658No Hit
GACTCAGGGTAAATATTGAATGACAAAACTCAGATGGAGGAAAAAGAACAAA1040.16868329710967658No Hit
AGGCTCGGGGAATTTCGCAAGCTGAAGCTTGTAATGGAATCCTAATAGAGAT1020.1654393875498751No Hit
TTGACAGGGAAATATATTAATTTTATGTTTGAGGCATATGATAAAATGAAAT1010.16381743276997437No Hit
TGGTTTGGGGAGCCGTACGCTCTTTACAGATACTAATGTCAAGAGTTAAACC1000.16219547799007364No Hit
TTTAATGGGGGAGGAGCAGGCACATCTTACATGGCTGGAGAAGGAGGAAGAG1000.16219547799007364No Hit
ACACAGAGGTAGAATAGATGCCAAAGTCATTTATTCAGTCCTTAGTTTTCTT990.1605735232101729No Hit
AATTTGGGGAAGGGAACTGAGAGTTTTAAGAATCAGAGGGTTATCAACAGTG980.15895156843027217No Hit
TAAATAGGGGTGAGACAGACAATATTATCCTTATTGTACAGATGGGGGGGCT970.15732961365037143No Hit
GACCCAGGGAGTCTAGGGACTGAGGAGTCGCCGCCGCCCCGAGTCCCGGTAC970.15732961365037143No Hit
GTTTTAGGGTCCGATCTGCCATGGCCAGTCACCCGGAGCCTTCAGCTGGCAG960.1557076588704707No Hit
GTCTTGGGGTGGATCTTGCACCAACATCTGGTACTTCAGCTAAGACACCCTC960.1557076588704707No Hit
TGACTCGGGGCATTGGGGATTACATTTCAACATGAGATTTGGGCAGGGATAA960.1557076588704707No Hit
CTAATGTCGGGAATCCGGTCACCTGCAATGTCTGGAGGAATTTTTGCTATAC950.15408570409056996No Hit
TTTGTGGGGAATGAACAATCAAGATGCCATAGAAAAGGCTGTTAGTAGAGGC950.15408570409056996No Hit
ATGACTGGGCATGTCATCAAGTAGAGAAAATATTTTTATTTTTGCCCAATAT950.15408570409056996No Hit
TTGATCAGGGACGCTCCTCATACTAGGCCTACTAACCAACACACTAACCATA940.15246374931066922No Hit
GGTAACGGGGCCCCAGATGTCAAGTAATGTTGGATTTTCTATAATAATTTAG910.14759788497096699No Hit
TTAGCCGGGAAGCTAAGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCT910.14759788497096699No Hit
CATGAGGGGGTTATTTTGCCGTTTGACATTTTTTATGGTTCATTTATTTTTA910.14759788497096699No Hit
GCTATAGGGTAAGCTGATAAGCAACTTCAGCAAAGTCTCAGGATACAAAATC900.14597593019106625No Hit
TACGTGGGATATGAGCTCCGGCAGGGACTGAGGCTGGCAGAGCTGGCTCTCT900.14597593019106625No Hit
ATTCGCGGGTGAACCCAAGTAGAGAAGATAGATCTTGGAAGGAGAGATCCAT900.14597593019106625No Hit
TGTGCTGGGTAGGGCTAATGAGTCATTGTTCCTTAATATCGTTTTCAATATG900.14597593019106625No Hit
GTTCCAGGGAACCTATTTTTAAAGAAACCTTTTTTGCTATTGACTGTTTTTT870.14111006585136407No Hit
AAGGTAGGGGAGGGCAAGACCCCAGAGCCTTAGCTGAGCAAGCCCAGAGGGG870.14111006585136407No Hit
AGTTCTGGGTAAGACCAGACCACAGAGGCAGAATGAAGCAATAGCTCCAGCA860.13948811107146333No Hit
ATTGATGGGGTGAGCGTGTGGGGAAAATTACCTCACTTTCCAGAACTTCCAT860.13948811107146333No Hit
TCGAGCGGGAATATCACATAGTTGGCATTGCTGAATAGTTTCATCTCTTTGG860.13948811107146333No Hit
AGTAAAGGGCAGCCACCAGGCTCCCTGTGTCCCTTCCATGGGAAGGTCTTCC850.1378661562915626No Hit
ATGCTGCGGGTGTTCGATTGCGGGTTCTCTGTCTCTGCGGAGGTGCCTTCCC850.1378661562915626No Hit
GTATATGGGCAGCTTGGGCATATGAGGAAGTGAGAAATAGGGTTTCTTTCTA840.13624420151166186No Hit
GTATCCGGGTATGAAATCTGTATAAGTGTGTCCTTATACAAATTTTAGGCCA830.13462224673176112No Hit
CTTGCTGGGCTCAGCCCATGGGGAGAAACGGAGGCCTGAATACAGGCGGTCA830.13462224673176112No Hit
ATATATGGGCCGATCTTACTGAGGGATCTTTGCAAAAGTGTAGCATTAAAGC830.13462224673176112No Hit
CTAAAACGGGATGACATTTATTTATTGTATCTGAATGATTTATTATGATATT830.13462224673176112No Hit
GATTCTGGGAGGACATATGAAGAGTGCAATGGCAAAACAAGAGCAAAAAGCA830.13462224673176112No Hit
TTAGTGGGGGGATGGCAAGTTGGATCCCTCTACCTTCCGAGGTCAGGAGTGC820.1330002919518604No Hit
GGCACTGGGACTACTCAGATTGCCAGCTTTAAGACTGATGAATGCTACCATC820.1330002919518604No Hit
AACGAGGGACTTGTTTTTTTCTTAACTTTACTCCCACTTCTACCTAACAGGG810.13137833717195965No Hit
TGAGTAAGGGGATAATTACGTTTAAAGTGCAGTCATTTGTGGTTAGAATCTT810.13137833717195965No Hit
GTCAATGGGTGAGCTACACATTATTTAGATATTTCCCCTAGAGATTAGAAAC810.13137833717195965No Hit
AGTTGCGGGCACTTTTTTGTATTTTTGCCATTTGAAAAGGTTGTGGTGTAGT810.13137833717195965No Hit
AAGACTGGGATCCATCACTGTGGGGATTGCTTAGTGTGTTAAAGCATGAAGC800.12975638239205892No Hit
ATAGCAGGGGTTTTTATACCTTCCCGCGCGGACGCCGGCGCTGCCAACGGAA800.12975638239205892No Hit
GCTACCGGGGATATTTCAAATTAGTGAGAAGGAATGGTATATTTAATAAGCA800.12975638239205892No Hit
TCAATGGGGTGACTCTAGTTAACAATTTATTGTATATTCTTAAATAACTAAA800.12975638239205892No Hit
AACTTGGGGCAGTGGCGTGATCTTAGCTCACTGCAAGCTCTGCCTTCCAGGT800.12975638239205892No Hit
ACATTTGGGTATCTGCATCTTTAGTTAGGCTGGTCTCAGGTTGCTCATAGGT800.12975638239205892No Hit
CAACCGGGGAATGGTGCATACAAGGCCATCCCCGTTGCCCAGGACCTGAACG790.12813442761215818No Hit
TTATCGCGGGGGAGAAGAAAAACTAGAAGTCAGAAATGACCCAGGGCTTTGA780.12651247283225744No Hit
CACCGCGGCCTGAAGATGTCGGCCACCTTCATCGGCAACAGCACGGCCATCC770.12489051805235671No Hit
CTACCAGGGGGAAGACTTGCCATTTTGTTTGGACAAAGTTGCAATTTAAATG770.12489051805235671No Hit
ACACCAGGGACTTTGACCGCGATGATGTGGCTTTGAAGAACTTTGCCAAATA770.12489051805235671No Hit
ACGAGTGGGCGATCTGAAAGTGGGGGAGCAACACGGCCAGAGTGAGTCCTGG770.12489051805235671No Hit
TAAGGGGGTGAGCACACCATTTGTGCTGCTGCTGTTGTCGTGAAATATAATA770.12489051805235671No Hit
CCAACTGGGAGGTCTCAATTTCTCACTGGAAAATGAGTCTTTAAAATAGTAA770.12489051805235671No Hit
CTTTAAGGGTCCGGACCGTTATTTTAGAAATTCCTGAACTCCTCAGTCCAGT770.12489051805235671No Hit
TCGGTGGGGGCATCTAAAATGCCATCAGCAATATACTAAATGCTAAGAATAT760.12326856327245597No Hit
CACAAGGGGAACCTAAATTTTGCTTGGTATTTACTATTGCTTTTAAAATAGA760.12326856327245597No Hit
GAACTTGGGATGTTCATGATAGGCTATTTTTGGAGCTCTTCTAAGATAGACA760.12326856327245597No Hit
ACTGCTGGGCAAACCTCTCAGGCATTATTAAAAGGATAATAAGGAAATACTA750.12164660849255524No Hit
CGTTTGGGGAGGCGTGGGAAAGATGAATTACAAACAGTTCTTGAGATCAAGG750.12164660849255524No Hit
CCTCTAGGGGCTCTTCTGAGGATCCGGCAAGATGGCAGAAGTAGAGCAGAAG750.12164660849255524No Hit
GCGGCTGGTGTCCTACCCCTTTGACACTGTTCGTCGTAGAATGATGATGCAG740.1200246537126545No Hit
AAGTCAGGGTTCATCATCTTAAGTATTGTAAGCTGCTATGTATGGATTTAAA740.1200246537126545No Hit
GTACGAGGGGCCTCTCGTCTCCTTCCTGGTTTGGGCGGAAGCCATCACCTGG730.11840269893275375No Hit
TATTACGGGTACATTTTGGATTAAACCTGTTTCCCGGTTATGTGTAGGGAAC730.11840269893275375No Hit
ATTGTCGGGAAGAAATCACCTGAAGGTACAAAACTCACTGGTAATAGTACTC730.11840269893275375No Hit
GCGCAAGGGGGGCTGGGAAGAGAGGCGAAGCCAGGTCACCTTTCAAGGACCC720.11678074415285301No Hit
TGTTAAGGGTAATGAGATTTTACCGTGCAGCTAGTGTGTCCTTTACTCACCC720.11678074415285301No Hit
CAACGAGGGCTCTGGCCTCTCCAGTCCTGCTTACTCCTCTATTTTTAAAGTG720.11678074415285301No Hit
GTTCAGGGGCGATCTTTGCGTGGGTATACTATTATATAATGATTTTATGGTT710.11515878937295228No Hit
ACCATTGGGGTAGGTAAATAACAGATGGCAAGAATGGGAGATGGGAATGGGG710.11515878937295228No Hit
GTCAGTGGGGCAGAGATTGGTACAAGCGCAATTTTGCCATCACCTTCTTCAT710.11515878937295228No Hit
ATGGATGGGAAGTGAAAAGAAAAAAATCAGTGTAAGTTAAAGGGCTTATATA710.11515878937295228No Hit
CTCTCGTTTCGGGAAGTGAGATACAAAAGTAATTGCAGGCCCAGGGCTGATT710.11515878937295228No Hit
ATTACAGGGTATGACTTTTGCCTCATACCTTGAATCCTAAAAGTTTTCGCTG700.11353683459305154No Hit
CACCAAGGGTCTGCATCAGGGTACTTTCATGTCTCTATATTCATTTTTCACA700.11353683459305154No Hit
GTTACAGGGTAAGATAATGAGCATAACCCTCACCTCCAAAAGTTTCTTCATG700.11353683459305154No Hit
CTAAATGGGTAAATGAACTAGCTTAATATTATACTATACTATACATCAATAC700.11353683459305154No Hit
GTAAAAGGAAGGAAAAACTAGCACCCTTCTGTCCACTCAGCAATAAGAGGGA700.11353683459305154No Hit
CAGTTTGGGATCACCAGCTAGAGGTTATGGACTATACACACCTATTATTATA690.1119148798131508No Hit
TTGGCCGGGGAAAGTAGATAGATCATTGGTTCAACCATTTTAGTGTTTTTGC690.1119148798131508No Hit
TGCTGCGGGTGACTCGCAACAACTTTCTTTAGAATAATTCCCGCATGAGTCC690.1119148798131508No Hit
TTCTTTGGGGCTTTAGCCTGAGACTTGCCATAAGAAACTGCTGAGTACTTGG680.11029292503325007No Hit
GTAGTTGGGGACCCTTGGCCATAATATGATTTATCTCCACACTAGCAGAGAC680.11029292503325007No Hit
CCGTCCGGGAAGGCACTGAATATGCTTCATACAGAACAATTATCTGACTACA680.11029292503325007No Hit
TACCACGGGAAGTCTGGAGATATCATCATTGTAGGCCGCTTTCAGCTTTCAT680.11029292503325007No Hit
AATAAAGGGCACCAGCAGGTGAAGTGCATCATCACAGAGGAGAAGGTGTGCA670.10867097025334933No Hit
GGTTAGGGGGAATGAGTAATGATCATGTTAGGGTTAGGGTATTTACAGTATC670.10867097025334933No Hit
ATGATAGGGATGATAAAGGGTCAATTCACCAAGAAGACATAACAATCCTGGA670.10867097025334933No Hit
CTTTTGGAGGGAAGTGTTTAAAATTATGCTCTGTAACTCTGTACTGCTAGTA670.10867097025334933No Hit
TTAACTGGGAGCCAGATCTTATCTATGAAATTTCCCTTTGTAGACACACACA660.1070490154734486No Hit
CCTTGAGGGCTGTTCACTCATTTGAGGACAACTGTACTACCAGAAAGGGAAC660.1070490154734486No Hit
TAACCCGGGATGGTTCAATGTACTTACAATGGAATCCCCACTAAATTCTGTT660.1070490154734486No Hit
ATAGTGGGGGAAATCCTTAACCAATTATAATAGCTTTTTTTCTTTATTTTCA660.1070490154734486No Hit
GCCCTAGGGCATTTAAATTGGTTATTTGTCTTATTATTGGGTTGTAAGAGTT660.1070490154734486No Hit
AGCTAAGGGATCTCTGTAGATATTCTGTTTTATTTTGGTCATCTTTAGAAGT660.1070490154734486No Hit
CTGTTCGGGGGACTAGCGAGGAGGAGTTGAGAGAACGGAGCGGACGCCATGG660.1070490154734486No Hit
ATGTGGGTAGCTCATGGATTGAGGTAAGATTTAAGTAAAAAGCGCCTAGGTT650.10542706069354786No Hit
AGTTTGGGGTCAACACTGGCAGCCACCGTCTCACCCCTGCGGAGGAGCGCTC650.10542706069354786No Hit
TAAGCAGGGATTGTATGCATGTATCAAAATATCACTTGTACCCCACAAACAT650.10542706069354786No Hit
GACCATGGGCCTGTAAAGCCCTTAATCCAAAAGCATTGTGCTCTTAATCTGT650.10542706069354786No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAGGACTC650.10542706069354786No Hit
CTTATTGGGGCCTTTATGGACCACATACGGATCTTCCAAGAGCAAGTGGAGA650.10542706069354786No Hit
GTACTTGGGGGCTTTGCGGCATGGCGATGGCGGAGGGCGAGAGGACTGAGTG640.10380510591364712No Hit
AATGTGGGGACTGTGAGGCACCAGATCCTGTGCTTCTGCAGGCCATTTTCTG630.10218315113374639No Hit
TGTCTAGGGAAGAGGTTTAATGGACTCACAGTTTCATGTGGCTGGGGAGTCT630.10218315113374639No Hit
GGGTAGGGGGAGGCTGCCAACCATTTTTCTGAAGTGATTATACTAATTTATA620.10056119635384565No Hit
CAACCTGGAGATCTCAGCCTCAGTTCTCTTGTCTGTAAAATGGAAGAACTGG620.10056119635384565No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)