FastQCFastQC Report
Sat 31 Dec 2022
SRR5844735.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844735.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143448
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCAGGACTCATCTCGTATGC10730.748006246165858No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGGACTCATCTCGTATGCCGTCT10500.7319725614856952No Hit
TCTACAGGTGGGAAATAGTTCTCATTGGCTCTAGTACTATTTGTTAGCTCCA2120.14778874574758796No Hit
AGAATCGGGATGCTTCATGAAGTCCATCACACATAAAGGCAAAAAAAGAACT2010.14012046177011878No Hit
ATTATAGGGAGATGGAAGAAGAAAAGCAGCAAACAGAAAGGGTTACAAAAGA1990.13872622831966985No Hit
GCTGTTGGGGATTTGAGCAATTTGTATAACATTTTATTCTGCTTTTTCTCCT1960.1366348781439964No Hit
GATCTGGGGCATTTCAACAGCTGAGGCAAGCCAAGTGATCATTAATAAAGCT1940.1352406446935475No Hit
CCGCGGGGGAAATAATTTATTATTACTAAGTCCTCAAAAGCAATTGCAACAA1820.12687524399085384No Hit
GCATCCGGGAACTGAAAGGTTTTTTTAATAAAATGCCATTTTGTGGAAACTG1810.12617812726562935No Hit
GTCTTTGGGGACTCGTGTCTCGCTACCAGCTCCCCGCTGCCCTGCGCTCGGC1810.12617812726562935No Hit
CATACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1790.12478389381518042No Hit
CTGTTGGGGCCTTTCCGTCTGGCGGCAGCCATCAGGTAAGCCAAGATGGGTG1770.12338966036473148No Hit
AATCGCGGGACATGAAGCTTTTCCGTGCCCAGAGGAATCTCTACATTGCTGG1770.12338966036473148No Hit
GTGCCTGGCCATTATGTTTTAAATATGTCTCCGTTTCTATTTCTAAATATTT1770.12338966036473148No Hit
CAGTTTGGGTGGCGGGGGGATGGGTCAAAGACTTAACAGTAAGAACTTAAAA1760.122692543639507No Hit
ATTCAAGGGCGTCATCACAGCAGCTTTGATGAAGAAAATGGATGCACCTCTA1760.122692543639507No Hit
TCAATTAAGCCTCCGCAGTTAGGATTTCTATTATTTGCATCCAAAAGCAATT1760.122692543639507No Hit
AGCAGCGGGATTTCTCAATTACTTTACATAGCCAATTCAGCGGATTAATACA1760.122692543639507No Hit
CTGTGCGGGACTTTAAAACATATCTACCATCTGATTGGCTGGTACCGAGAGC1760.122692543639507No Hit
GGCGCAGGGGTAGTAGTATTGAACCAAAAATAGTCAAGTAAATAATGTCTCA1730.12060119346383358No Hit
CAACTAGGGGATGGAAAGGAAAAACTGGTACCAGCCACTGCAAAAATGTACT1730.12060119346383358No Hit
CACACGGGATTTTACAGGTGAGTAGACTGAAGCACAGAGAGGTTAAGGACCT1730.12060119346383358No Hit
CTTTGCGGGCTGTTCCTTAATTCTTCCACTATAACTTGTGTGTATTTCTTTA1710.11920696001338466No Hit
ATAACGGGGGACCTGTATGAGGCCTCACAGGAGAGTGCGTGGCTCGAGTGGG1710.11920696001338466No Hit
TCGCCTGGGCAGTCCTCACTTCATATCTCTCTTGGATTTAAGACTGTCCAAA1700.11850984328816018No Hit
TCCGCGGGGCCCAGCACTTTGAGAGGCTGAGGCGGGCGGATCACTTGATGTC1700.11850984328816018No Hit
TTCTTAGGGATTGAAAGCACATTTAGATTATTAATCAAGTTAATAAAAGATT1670.11641849311248675No Hit
GTAGATGGGATGGGGGTGTGGGTAACAAACTTTGATCGTGTATGAACAATAA1660.11572137638726228No Hit
CAACAAATGAGCTGTGTTAATACATAAGAATAAGAAGGTATGTATATTAGTG1650.11502425966203782No Hit
TTTATTGGGGCTGGGCGCTATGTTCGTGAGAACTGCAAACCGCTGCGGCGGT1650.11502425966203782No Hit
GAACAGGGGAAGTACATTTGCATTAAAAAGTGCAACGGACAGAAAGATTAGC1650.11502425966203782No Hit
GCACTGGGGTGATTCTTATGATGTTTATGAACAACAAATTATCCTTTAATTA1640.11432714293681334No Hit
AGCGTAGGGCAGAGGCTGGGTGCAGTCAAGACAAAAGTAAAATGGTTTACCT1630.11363002621158887No Hit
ACAAAGGGGCATGTTCATGAACTTCTGCTGTACATTGGAATAGGAGTTAACA1630.11363002621158887No Hit
TATAGGGTAAATCTACAACAACAATGGAAGACATTCTTATTTTTGCAACTGG1630.11363002621158887No Hit
CAATTTGGGAACGAGAGGCAAGTATTGACTCCTCACATAGACAACAAATGAA1630.11363002621158887No Hit
CTCATCATACACAAAAATCAAAATGGATTAAAGACTTAAATCTAAGACCTCA1620.11293290948636439No Hit
ACGTAAGGGGAATGCAAATGTCCTTTGTGGTGCTTGTTCCTTGAAAATGTTT1620.11293290948636439No Hit
ACGCTTGGGGGAGAAAGTTCATTAAAACATTCAGTGTATCTTGAGAAATTTA1610.11223579276113992No Hit
GGCAACTGGGAACATAATATGTTGTCTTTTATATTTTTATTTAAATCTGGAT1610.11223579276113992No Hit
AATCAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1610.11223579276113992No Hit
GACTAGGGGCTGAGTGTACAGGAAATGTAAACCATTTAAATTAGCCTTTTAA1610.11223579276113992No Hit
TCAGCGGGATCCTGACATTAATGAGTCTTCTTATTCATTAACATGAAATATC1600.11153867603591544No Hit
GCACGAGGGATAACTTTGAAAAAAACATAATCTGTTAGATCGGTGAAGGATT1590.11084155931069099No Hit
AGCATTTCTGCATGGTCAAGTGAGCTTTATGCTCATGAGCTTTAAGTATATA1590.11084155931069099No Hit
GTACATGGAGAGGCCAGGATGTCAGTTCCAGGATGTGCGAGAAACTTATAGA1590.11084155931069099No Hit
GTTTATGGGCGGCAAGAATGGCTTGGAGGGGCAGGAGTAGAGGAGAGAGACT1590.11084155931069099No Hit
TTATTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1590.11084155931069099No Hit
TAAGGCAGGGCCAGCTGGAGAAGCTGAGGGCTATTTTTGCAATTATAATCTG1590.11084155931069099No Hit
AGCCAAGGGTCACATTGATTGAGAAGAGCCAATGGGTAGAATGGCCAACCAT1580.11014444258546652No Hit
GTATTGGGGGGGGTCCCTTGAGATTCCAATGAATTTTAGGATAGATTTCTGT1570.10944732586024204No Hit
TGAAGTCGGGAAGATGTAACTGTTGGAAACTCTACCCTGATTTTCCTTTCTA1560.10875020913501757No Hit
GGTTCTGGGCAATTTGGAAAAAGAATTACAAGTGCTGAGATCTCTTGCAGAT1550.10805309240979309No Hit
CATTGTGGGATTTCACCATATGTGTTCCTATCTCCAATCTATTCTGTCATTG1550.10805309240979309No Hit
ATCAATGGGGAGAAGGCGACGATGACCGGACTGTGTGCCGGGAGATCCGCCA1540.10735597568456862No Hit
TTACATGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGG1530.10665885895934415No Hit
TTTTAAGGGAGAGCACTGAAAGGTGTTAAATTGGGGTATGTGGTTTGATTGA1530.10665885895934415No Hit
CGTATGGGGAACTTAATCTTAACAGGTGGTTGTCTTCATCTTTTTATGCCTT1530.10665885895934415No Hit
TCGAATGGGAGGGTGATGATCTTCTGAAGCTCTTGGATGTCATTTGATCGGA1530.10665885895934415No Hit
GTCTTCGGGAATGCAGAATTTGTGAATAGAAAAGATTAACTGTAAATCACTT1520.10596174223411968No Hit
GTGTCCGGGGAGGGCTTTTGCTGGCTGCTGCTGCTGCCCGTCATGCTACTCA1520.10596174223411968No Hit
GCTTCTGGGGTCTTTCAAATTCTGAGAAAAAACATGAGGTTGGAGATGTCTT1520.10596174223411968No Hit
GCGGCTGGGCGATCTAGCATGGGGATCATTTTTTCTTTAGGCTATATAAAAT1520.10596174223411968No Hit
GTAGACGGGCGTCCTGCCCAAGGATGGAGTGCAAACATTCAAGGCCAGGCGT1510.1052646255088952No Hit
ATCAGTGGGATCCAGAAGGACCTGGAAGAACTGGAAGTCATTTTACGAGACT1510.1052646255088952No Hit
AGCTCTGGGGAAATCCAAGTAAGATTTTCACAGTGGAAGCTTTGCAACAGTT1510.1052646255088952No Hit
CTTTGAGGGCTGGCAGAATGTGTGAATGCCATATCTGTAGATGACGATGAAT1510.1052646255088952No Hit
CATTGTGGGGAGTCAATAGCTTGTCTTCGTCCATCTGTTGACAAATGACAGA1500.10456750878367073No Hit
TACGCTGGGTCACATATGCCTAAAGTTTTCCCATTAATTATGAAAGCCTTGT1500.10456750878367073No Hit
GTGGTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1500.10456750878367073No Hit
CTATATGGGTTCTTATACCTCCTAGCCATAAATACTGAAGTCTTATTTTGAA1500.10456750878367073No Hit
GTCTCGGGATGTACGCAAATATTGGCTGAGATTTAGCAGAGGTATTGAACCA1500.10456750878367073No Hit
TAATTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1490.10387039205844625No Hit
TGACGTCGGGATGTGAGCTCTATATAATTTTCTTCTTTCAGGATGACTTATA1490.10387039205844625No Hit
ACGCCTGGGATATCCCTGGTGATAAGTGATGTTGAGCATCTTCCAGAATAGT1490.10387039205844625No Hit
TTACATGGGGAAATTTTTTATAAATCCTACAATTAAATATTATTTGCTTAAT1490.10387039205844625No Hit
CATCGTGGGGCAATAAGTCCATGGAGATCGAGGTGTTGGTGGACGCCGACCC1480.1031732753332218No Hit
ACTAAGGGGAATGTAGGAGGTGACTATTTTTGTTATAAAAGGATTTCCAATG1480.1031732753332218No Hit
TAGCCATGGGCACAGACTTTAAACTGTATTAACAGATTCTACTTTCTTCTAG1480.1031732753332218No Hit
AAATGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1480.1031732753332218No Hit
TAAGTTGGGTATCTAAGAGCAGGTTTTCTGTATAAATCATTCTGCTAACTTT1470.10247615860799733No Hit
GACATAGGGAGACATAAACTAAAAGAGTTGTTAAAGGATAGTTTTCAGGCTA1470.10247615860799733No Hit
CAAACAGGGGACTGGGTCACTGCACTCTAACCTGGGTGACAGACGGAGACCC1470.10247615860799733No Hit
CGATAAGGGGGGACGCTTCGGCCGTAACGATGATCGGAGACATCCTGCTGTT1470.10247615860799733No Hit
AAAGACGGGATACTCAGAATTAAAGAGAAATTGGGAAAATGGTTCTTTGTTC1470.10247615860799733No Hit
ATAAGTGGGATTCTACTGTTATATTAATAAACTGGTGGGAGTCTTGTTTCAC1460.10177904188277286No Hit
AGTCACGGGTCCCACTCATGACATGTCCACCCTCATGACCTCATCACCTCCC1460.10177904188277286No Hit
ACAGCTGGGCCGATCTTCCGTGGGATTGTAATGGCTAGAAGAGACCTGCCAG1460.10177904188277286No Hit
ACGCCAGGGGAAAAGTGACTACTCAGATATGTTGGAAGTCACATCGAAGACT1460.10177904188277286No Hit
TTGGTAGGGCTCTCTCCTGCTTGCGCTTAGTGTCCTACATGGGGTTGTGAAG1460.10177904188277286No Hit
GCTGAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1450.10108192515754838No Hit
ACTGGTGGGTTCCTTTTTCTAGATAAGAAACTGTGGTGGGCAGACCAAAACT1450.10108192515754838No Hit
CGTACAGGGATGAGAAGGAGTCCCGACACCTGATGCCTTACTGGGGGATTTA1450.10108192515754838No Hit
TAAGCCGGGAATATGCAGAATATAAAGGGGACTCAAACAACAGTAAAAAATA1450.10108192515754838No Hit
ACAATTGGGAAGAGAGAAATCTGAAAACCCCATTTGCTTTCTTTTCTCCCAT1450.10108192515754838No Hit
GACCTGGGGATATACTGGGTAGTTGGTCTCTGAATAAAGAAAATTTGCAGTT1450.10108192515754838No Hit
TTGAAGGGCTGGGGCATACAGGACAGTAAGACCTTTAGATTTTACCTTTTTG1450.10108192515754838No Hit
ACTTTGGGCAGGCATGAGCCACCTCACCCAGCCCAATTCCTCAAAATTTTAA1450.10108192515754838No Hit
TATTAGGGAAGTCAATTATTAAGCAGAACTTTCCCTTCATTAGACAACCTAG1440.1003848084323239No Hit
CGAAGTGGGGCCACTTCCGGTCGGCGGCGGCTCAGCAGGTGTTACCTGTTAC1440.1003848084323239No Hit
ATCTGTGGGAGAAGACTTAAATAATACTTATGAGTTTAAATGTAATTTTTAT1440.1003848084323239No Hit
CGACAAGGGTAAGGATGATAAGAGTGGTAGAAGAGACTATAGAAACAAAATT1440.1003848084323239No Hit
GTCACTGGGGAAGGCGATCTTTTAAAAAGAGATTAAACCGAAGGTGATTAAA1440.1003848084323239No Hit
ACGGTGGGTCTCTCACAGGAATGTGCATTATACATATATATTAAATATATCC1440.1003848084323239No Hit
CGCACGGGGCAGACGACATGATTGTATATCTAGAAAACCCCATTATCTCAGC1440.1003848084323239No Hit
TGCGACGGGTCATACTGAAGCCAAGAATATGAAGCAAAATTTAGGAGAAATG1440.1003848084323239No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)