FastQCFastQC Report
Sat 31 Dec 2022
SRR5844734.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844734.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143120
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCAGGACTCATCTCGTATGC11300.7895472330911124No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGGACTCATCTCGTATGCCGTCT10060.7029066517607602No Hit
ACGTAAGGGGAATGCAAATGTCCTTTGTGGTGCTTGTTCCTTGAAAATGTTT2100.14673001676914477No Hit
GTGCCTGGCCATTATGTTTTAAATATGTCTCCGTTTCTATTTCTAAATATTT2030.14183901621017328No Hit
TATTAGGGAAGTCAATTATTAAGCAGAACTTTCCCTTCATTAGACAACCTAG1990.13904415874790385No Hit
CATACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1970.13764673001676914No Hit
ATTATAGGGAGATGGAAGAAGAAAAGCAGCAAACAGAAAGGGTTACAAAAGA1800.1257685858021241No Hit
CAACTAGGGGATGGAAAGGAAAAACTGGTACCAGCCACTGCAAAAATGTACT1790.12506987143655673No Hit
GCGGCTGGGCGATCTAGCATGGGGATCATTTTTTCTTTAGGCTATATAAAAT1790.12506987143655673No Hit
AGCTCTGGGGAAATCCAAGTAAGATTTTCACAGTGGAAGCTTTGCAACAGTT1780.12437115707098938No Hit
TTACATGGGGAAATTTTTTATAAATCCTACAATTAAATATTATTTGCTTAAT1780.12437115707098938No Hit
CTCATCATACACAAAAATCAAAATGGATTAAAGACTTAAATCTAAGACCTCA1770.12367244270542202No Hit
TAAGTTGGGTATCTAAGAGCAGGTTTTCTGTATAAATCATTCTGCTAACTTT1770.12367244270542202No Hit
TACGCTGGGTCACATATGCCTAAAGTTTTCCCATTAATTATGAAAGCCTTGT1770.12367244270542202No Hit
CAACAAATGAGCTGTGTTAATACATAAGAATAAGAAGGTATGTATATTAGTG1760.12297372833985466No Hit
GGCGCAGGGGTAGTAGTATTGAACCAAAAATAGTCAAGTAAATAATGTCTCA1750.12227501397428732No Hit
CTCATCGGGTCAATACCTTTTCAGCCACGAATTCCTTTTCGGGTGTTTATCC1740.12157629960871995No Hit
ATTCAAGGGCGTCATCACAGCAGCTTTGATGAAGAAAATGGATGCACCTCTA1730.1208775852431526No Hit
AGAATCGGGATGCTTCATGAAGTCCATCACACATAAAGGCAAAAAAAGAACT1710.11948015651201789No Hit
GATCTGGGGCATTTCAACAGCTGAGGCAAGCCAAGTGATCATTAATAAAGCT1710.11948015651201789No Hit
TTATTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1690.11808272778088316No Hit
TTACATGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGG1680.11738401341531582No Hit
GCTGTTGGGGATTTGAGCAATTTGTATAACATTTTATTCTGCTTTTTCTCCT1680.11738401341531582No Hit
TTTATTGGGGCTGGGCGCTATGTTCGTGAGAACTGCAAACCGCTGCGGCGGT1670.11668529904974848No Hit
TATTCAGGGTATCATTTGGTTATCTTACTGGGACATTTTCATTGATATTCTA1670.11668529904974848No Hit
GCACGAGGGATAACTTTGAAAAAAACATAATCTGTTAGATCGGTGAAGGATT1660.1159865846841811No Hit
TCGCCTGGGCAGTCCTCACTTCATATCTCTCTTGGATTTAAGACTGTCCAAA1640.11458915595304639No Hit
CCGCGGGGGAAATAATTTATTATTACTAAGTCCTCAAAAGCAATTGCAACAA1640.11458915595304639No Hit
GTAGACGGGCGTCCTGCCCAAGGATGGAGTGCAAACATTCAAGGCCAGGCGT1620.11319172722191169No Hit
AGTAGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1620.11319172722191169No Hit
ACTGGTGGGTTCCTTTTTCTAGATAAGAAACTGTGGTGGGCAGACCAAAACT1610.11249301285634432No Hit
GTATCCGGGATAGTTTTCATTGTGCAAGTCTTATCCTTCTTTAGTTAAATTT1610.11249301285634432No Hit
GTAGATGGGATGGGGGTGTGGGTAACAAACTTTGATCGTGTATGAACAATAA1610.11249301285634432No Hit
ACAAAGGGGCATGTTCATGAACTTCTGCTGTACATTGGAATAGGAGTTAACA1600.11179429849077697No Hit
CTTAAAGGGCAGCAGTAAAGCCAGCATGTTAATTGCAGTGGTCTGACCTGCA1600.11179429849077697No Hit
TGCGACGGGTCATACTGAAGCCAAGAATATGAAGCAAAATTTAGGAGAAATG1600.11179429849077697No Hit
AAAGTGGGGAACTCCAATTTGAATTGAACTTAACTATCGGCTTTCTTACTGG1600.11179429849077697No Hit
TATCAAGGGTCATTCAATTAGAAACTGCAGTAGGGGCTGCCATCAAAAGTTT1590.1110955841252096No Hit
ACTGCGGGGACATATACTACATATACATATGAAACTGCAAGATTACTCAGTA1590.1110955841252096No Hit
CTATTCGGGCATTTTTCATTTTGCATTCTCATTTCCACCATCATAATTAATC1580.11039686975964226No Hit
CTTTGAGGGCTGGCAGAATGTGTGAATGCCATATCTGTAGATGACGATGAAT1580.11039686975964226No Hit
GGCAACGGGCAGCTTTGGTTACCATAGCCTTGAAGTATAATTTGAAGTCATG1580.11039686975964226No Hit
TTCTTAGGGATTGAAAGCACATTTAGATTATTAATCAAGTTAATAAAAGATT1580.11039686975964226No Hit
GTCTTTGGGGACTCGTGTCTCGCTACCAGCTCCCCGCTGCCCTGCGCTCGGC1570.1096981553940749No Hit
CAGTTTGGGTGGCGGGGGGATGGGTCAAAGACTTAACAGTAAGAACTTAAAA1560.10899944102850753No Hit
GCTGAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1560.10899944102850753No Hit
AATCAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1560.10899944102850753No Hit
TCCGCGGGGCCCAGCACTTTGAGAGGCTGAGGCGGGCGGATCACTTGATGTC1560.10899944102850753No Hit
AAGGCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG1550.10830072666294019No Hit
TCTACAGGTGGGAAATAGTTCTCATTGGCTCTAGTACTATTTGTTAGCTCCA1550.10830072666294019No Hit
GCTATAGGGACCTTAATCTAAGTAATCAGTAAGCCATTTAGTAAGCTCAACA1550.10830072666294019No Hit
GGCAACTGGGAACATAATATGTTGTCTTTTATATTTTTATTTAAATCTGGAT1540.10760201229737285No Hit
TTTGTAGGGGAGTGTGGCCCGGGCGTCCGCTCCTCAGCGGATGTGGCAGCCC1530.10690329793180547No Hit
AAGTACGGGCAGGTGCCCAGGAGCATGACAATATGCCCGAGGGACCGTAACA1530.10690329793180547No Hit
GCACTGGGGTGATTCTTATGATGTTTATGAACAACAAATTATCCTTTAATTA1520.10620458356623813No Hit
GACGTAGGGGATCTGCAGGGGGGTTTCTCTCTGTCCTTGACTCTTGGGTCTG1520.10620458356623813No Hit
GTCTTCGGGAATGCAGAATTTGTGAATAGAAAAGATTAACTGTAAATCACTT1520.10620458356623813No Hit
ATCAATGGGGAGAAGGCGACGATGACCGGACTGTGTGCCGGGAGATCCGCCA1520.10620458356623813No Hit
CGTACAGGGATGAGAAGGAGTCCCGACACCTGATGCCTTACTGGGGGATTTA1520.10620458356623813No Hit
CAGGCAGGGTGGCATTTAATGCCTGCTAAATTTTTGTCCGATTATATATAGA1510.10550586920067076No Hit
AGCCAAGGGTCACATTGATTGAGAAGAGCCAATGGGTAGAATGGCCAACCAT1510.10550586920067076No Hit
TCAGCGGGATCCTGACATTAATGAGTCTTCTTATTCATTAACATGAAATATC1510.10550586920067076No Hit
TGGTTTGGGCGACTAATTCTGAGGCAATGATGGACAGAGATGCTACTTCTTA1500.10480715483510342No Hit
GACACGGGCGAAGTTTTCTTAAGTTTCTGAAGATCAAGGCCTATATAATTCA1490.10410844046953606No Hit
TCGAATGGGAGGGTGATGATCTTCTGAAGCTCTTGGATGTCATTTGATCGGA1490.10410844046953606No Hit
ACGCTTGGGGGAGAAAGTTCATTAAAACATTCAGTGTATCTTGAGAAATTTA1480.10340972610396869No Hit
GTCTTGGGATTGAACTGTAACCTCATTCAAAACTTACCCATGTAATATTCTT1480.10340972610396869No Hit
TTAGAGGGATGCTTGCAAAATACTTTGTTAACAAAAGCTGCAAAGAGAGAAC1470.10271101173840134No Hit
CACACGGGATTTTACAGGTGAGTAGACTGAAGCACAGAGAGGTTAAGGACCT1470.10271101173840134No Hit
CTCCCAGGGGCAGCCGTCTGTGCCACCCAGAGCCGGCGGGCCGCTAGGTCCC1470.10271101173840134No Hit
ATACGCGGGAACCTGACTCCAAAATACACTACAAAGGCATAGTAACCAAAAC1470.10271101173840134No Hit
GGGTACGGGGGAAACAAAGAGTGAGTTTTCACACAGAGGTATTATTGTTTTT1470.10271101173840134No Hit
GCATCCGGGAACTGAAAGGTTTTTTTAATAAAATGCCATTTTGTGGAAACTG1460.10201229737283397No Hit
TCGTCTTGGGCGTTCTCCTCGGACCTTCGTAGCCATATCACTTGTATAAAAT1460.10201229737283397No Hit
TAAGCCGGGAATATGCAGAATATAAAGGGGACTCAAACAACAGTAAAAAATA1460.10201229737283397No Hit
CTGTTGGGGCCTTTCCGTCTGGCGGCAGCCATCAGGTAAGCCAAGATGGGTG1450.10131358300726663No Hit
GGTCGCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1450.10131358300726663No Hit
TAAGGCAGGGCCAGCTGGAGAAGCTGAGGGCTATTTTTGCAATTATAATCTG1450.10131358300726663No Hit
TTGGTAGGGCTCTCTCCTGCTTGCGCTTAGTGTCCTACATGGGGTTGTGAAG1440.10061486864169927No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)