FastQCFastQC Report
Sat 31 Dec 2022
SRR5844733.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844733.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151001
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGGACTCATCTCGTATGCCGTCT6210.41125555459897617No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCAGGACTCATCTCGTATGC6020.3986728564711492No Hit
ATAGCAGGGGTATGGATGTCTACAAAGGGATGTGCTGTAACTCAAGTATAAG2380.15761485023277994No Hit
TAATGTGGGTGCGTGCAAAGGCACGATTGATTTATCATGCTGGCTTTTATGC2320.15364136661346614No Hit
TGCTTAGGGATGAAAACTTGAAATAATAAAAAGAGAAAAAAACTATTTCCTA2310.15297911934358052No Hit
GAACATGGGGGTAGAAAAATAAAGCAAGGAAGAAGTAAAAAATGTGTATCTG2260.14966788299415235No Hit
ACTTCCGGGGTTGGCATATATGAAATGTAAACATATCTCTTCCCATCCAGGG2240.1483433884543811No Hit
GAAGCGGGATATTATCTCATCTGATCTTTGTAATATAACTGTAACTTTCAAT2230.14768114118449546No Hit
CATTATGGGAAGTTAGAAGAATTTTGAAGAAACAGAAAAGTAACTACATCTC2220.14701889391460984No Hit
TGCTCAGGGAAAGAACTTCTGACTTTTGAGGAAAATCTAGCTTTCCAAGTAA2210.1463566466447242No Hit
ACAACACGGGATTTTTCTTGCTGGGGTTGAGGAGAGAAAATATAGAATTCCT2210.1463566466447242No Hit
ACAGCAGGGATTCTATTTATTGAGAAAGTGATTTAAAATTATTATTTTTAAT2180.14436990483506731No Hit
GCTTTGGGGGGTAACGGGGCAGAGAGGCTGTTCGCAGAGCTGCGGAAGATGA2150.14238316302541043No Hit
TTGCATGGGTTACTAGGCAGAATTCACTAATGGAGTAGATATGTGGCATTCA2150.14238316302541043No Hit
CGCGCTGGGGGCGGGAGCCGGGGGGCGCAGCTAGAGAGCCCCGGAGCCGCGG2130.14105866848563917No Hit
TAACAAGGGGCTTGAAGAGACGCTAATTTTCTGTGGTTGAAGAGATGCTGTG2110.1397341739458679No Hit
GGCTCCGGGGATTCACCAGTCTGCCCTGTCACGTAGTTGAAGGAGTTGCATT2100.13907192667598225No Hit
CATAACGGGAACCTAGAGAGATTTTGAGCCTCATATTTCTTTGATACTTGAA2080.13774743213621102No Hit
TAGATAGGGAAGAATATCTTGAATGTTGTGTATATATTTTAAAAAGCACTTT2080.13774743213621102No Hit
AGAACCGGGATTGAGATCCTTTGCCCATTTTTGAATTGTGTTTTCTTTTTGT2080.13774743213621102No Hit
CATAGCAGGATAGCTTATTTTTAAATTTAAAAAATCAGTTTTTAAAAGTATA2060.13642293759643975No Hit
AGTAGCGGGTCCGATCTCAAAGAGGGCCTCTGACACATTCATGATTGATTGA2060.13642293759643975No Hit
ACTGCCGGTCCTGTCCAGCAGGGGTCTCCCAACAAGTGCTCTTTCCACCCCA2030.13443619578678287No Hit
AAGTACGGGAGGGTACTTTTATTGATGGATATGTTTTCCTTTTATTCACATA2030.13443619578678287No Hit
GAAATAGGGAGGTAGACATGAACTTTTCCTGCACAAAGCCTTGCTTTTAGAG1970.1304627121674691No Hit
GCAGCCGGGATGTGAACAGACAGGAATTTCTTGTGCAATGTGGAGCAAATGG1970.1304627121674691No Hit
ACGATGGGGGCGGGGAGAGGAGTACGGGTTCAGCGTGGACTGGTGGGCGCTG1960.12980046489758346No Hit
TAAAGGGGGACAGCTTGCCTTTGCTTTTTCTCACACACATTCCTAGCCTGAA1960.12980046489758346No Hit
TCTCAAGGGATATGCTGTTTTGTAGAGTGATGACAGAGAAACAGATACAGCA1950.12913821762769784No Hit
TACTTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1940.12847597035781222No Hit
TGCACATGGGACTGAGCATGTAGAATTTATTTATTCTAATTATAGGATTCTG1920.12715147581804093No Hit
TATTCTGGGGTTCCTGTGGACATCAGGAATGGCCATGGAACTTGATTTGGCC1920.12715147581804093No Hit
TTCAAGGGGAGTGGTATTAATTTGCAAGTAGGGAAAAGATACAGACAAGATT1920.12715147581804093No Hit
CCAAACCGGGCTGTTTAGAAAATGCCATCACAAAGGGGACAGCACATGCAAA1910.1264892285481553No Hit
TACCATGGGAGTACAGACCCTAAACTTGTCATTTGTTTTTCTCACAGAATAG1910.1264892285481553No Hit
ATTATTGGGAGCGCCCAGTAACTCATCCTTCATTCAGAGGTTTGCTGGCCTT1910.1264892285481553No Hit
ACTTTAGGGAGGAATCAAAACCTTCTTCACAAGTCCCTGCACCGAAGAGCCT1890.12516473400838404No Hit
ATTGCAGGGAGGGCCCCGGGGGCTGCGGAGGTGGCTGAGACGCGGCTGGCTG1880.12450248673849841No Hit
TTGGTAGGGTATCCAGGCTCCATGACAGTCTTGCCATAGAAAGAAAAATAAA1880.12450248673849841No Hit
GTGGACGGGGGTTTAGTGGAATAGAAATGCACAAGAACAATAGAGATCAGGT1870.12384023946861279No Hit
CATGCTGGGAAGCTTTTTTGATGGTTGTTAATTTTTTGATGGGGAGTATCAA1870.12384023946861279No Hit
CAGCTAGGAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCTAAAAC1860.12317799219872716No Hit
TTAGACGGGATCTAGCAAAAATATTCTTTGTAACTGAAGGAGAAATAAAGTC1860.12317799219872716No Hit
TGACAAGGGAACATATAACTTTAAGAAGTGAAGAAAATAATTGAAGTTAGAA1860.12317799219872716No Hit
GCATTAGGGAAGTGAAGTTAAAGGACAGCAGACAGTAACTCAAAGCTGTATG1860.12317799219872716No Hit
GAACACGGGGCTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGA1850.12251574492884153No Hit
TAGCAGGGGATCCAGTACTTGTGGACACTACACGTTTCAACCTCTCTACTAG1850.12251574492884153No Hit
TGTGTAGGGATGATTATACAGTGACACAATTTTCAGAACCTACTCTTGTCAT1850.12251574492884153No Hit
AGCAAAGGGATGACTGGAAAATAAAGTGTGCCAGACGTAAGGAATGATTCTA1840.12185349765895591No Hit
GGTCTGGGGACATGCAACCATGCCTAGCTAATTTTTTCTTTTCTTTTCTTTT1840.12185349765895591No Hit
GCGCTAGGGAAGGACATTTTCTACATTGAAAACCAAAAGGAATATGAAAATA1840.12185349765895591No Hit
CTCTTGGGGTCTGAGCTTTTACCACTAGACTCAAGGAAGAATAATTTTAACA1830.12119125038907028No Hit
TTCAATGGGAAGGTCTCTGAGAGTAGAAATCATGTCTAATCTTTGTATCCTA1830.12119125038907028No Hit
AATTGTGGGAGGAGTTCATTAGCCAACTAAGAAGAACCTACATCATTTGCAG1830.12119125038907028No Hit
ATCCCAGGGGAGAAGAAAACACATGCCTGTCACTCTACGGCAGCTGCCACCA1830.12119125038907028No Hit
TCTGCTAGGGGTGAAACAACATATTTGAAGTTCTGGAAAAAGAATAAATCAA1820.12052900311918463No Hit
ATGGTTGGGGAGCTCAAGTACATGAATGTTAGTTGTTATCACATACAGCAAA1820.12052900311918463No Hit
TGTCATGGGAGGTCAGCATGTGAAATGTTGGATTTGACATGTCAGGTAGGGT1820.12052900311918463No Hit
AAATATGGGAATGTTTCAGCATTATGATTTTCAAGACTTTAGGGATTTTGAT1810.11986675584929901No Hit
GGTTACGGGAACTCATATAAAAATTATATTTAGAATTTTTAAACATGTACAA1800.11920450857941338No Hit
CTCAAGAGGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAG1800.11920450857941338No Hit
GGTTCCGGGCAAAGAAAAATGGTGTGCTTTAGGGAGGGAAAAGACAGATTTC1790.11854226130952775No Hit
CCCATGGGATGGAGACCCGAAGGGACTCAGACGGAGCCGCCGTGTTGGCAGC1790.11854226130952775No Hit
GACGCAGGGTATGTGTCAATCGACTATGTTATCCGTAAGACTTCTGGTCAGC1790.11854226130952775No Hit
ATCTTTAGGGAGGGAAAATCAGACAAAACAAAAGAAACAAACCAAAATATCC1780.11788001403964213No Hit
TCAGACAGGGATTGGGGAAATATCTGAGAAGAGAGGTCTTGTTTTATCCAAC1780.11788001403964213No Hit
ATGTCTGGGCTATGTCTTCTTTGTTCTCCTTTTCTTCTTTTTCTGCTTTCCC1780.11788001403964213No Hit
GGTAGGGATTTAATAGATTGTTATCTTCTTCCAGATTACATCAATCTTCCAG1780.11788001403964213No Hit
GCCATGGGGGGCTTTTACGTGGGGGCTGGGTCATGACTTCCAAAGCAGGTGT1780.11788001403964213No Hit
AGATTAGGGACTGAATTGAGAGATATGCTCCATGTTTAAAAATATTTGTAAT1760.11655551949987086No Hit
CAGAACGGGTCTAACCAAGAGCCACATGCTTACCATGTATCAACCAGTAGCT1760.11655551949987086No Hit
AACTCAGGGCCGTCTCAAAGAAAAAAAATTGTTTCCTTGCTGTGATTTTGGT1750.11589327222998524No Hit
TGCTATGGGCTTGGAATAAAATGTGTTTCATAACTTAGCTATATTAAATACA1750.11589327222998524No Hit
ACTGATGGGAGGCATTGAAAATAGGTGGCCTGACTGATTTAAACATTCTTTT1750.11589327222998524No Hit
TACGTTGGGCAAAAATCTTACGATGATAATAGTGAGCACCTTATTGCTAAGT1740.1152310249600996No Hit
AACATAGGGAAATGCAAGAGGGAAGTTGTTTTACTGTTAACTGATTATAGAT1740.1152310249600996No Hit
TGCTGGGCAGAAAACTTATTACCCTTCTCCACCAAGGTAACCAATGAATGTT1740.1152310249600996No Hit
AAGTATGGGAGGCCGACCAGCACTTGGGATCTGATGGGACACGGCCAGTGCC1730.11456877769021397No Hit
TAGATGGGGAACTGTTGTGATCACAGTGCATTGTAGCCTGGAATTTCTGGGC1730.11456877769021397No Hit
GCACAAGGGCAAACTCATAAACCAGCAAAGAGAAAAGTAATAAAAACTGTAC1720.11390653042032835No Hit
ACTAGGGGTGAGACTCCTTTTAAGACTAAACCATTTGCATTAAACTAGAGCC1720.11390653042032835No Hit
CACACCGGGGAGAGGAGAATTGTTAGTAACATCAAAGGTCAGAATGCTTCAG1720.11390653042032835No Hit
GCCACGGGACATGGAAGTGGCTTTAAATCAAGCACATTAACCTCAATGAACT1710.11324428315044271No Hit
AAGGGAGGGCTGCCCAGCCAGCTCAGGCCTCACTGAGGGCCTGGGTGACTAC1710.11324428315044271No Hit
ATTGTAGGGATGATAAAGCTGTTGCTACATCACAGCTTCAGAAAAAACTTGG1710.11324428315044271No Hit
TCAGTAGGGAATAGAAATACTCGGAGACTAACTTGTGAATAACTGCTTACAA1700.11258203588055708No Hit
CGGTTAGGGATCTCTAGATTTTTTATAATACTTAATACAATGTAAATGCTAT1700.11258203588055708No Hit
ATTGAAGGGCAAGCCAAGACCAAGTTAGGTTTATGTGTTTTTGGCTTAACCT1700.11258203588055708No Hit
AAACAGGGGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTG1700.11258203588055708No Hit
TGTTAGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1690.11191978861067144No Hit
AATTTGGGCAGCTCAGTATCATCCCATCTCCCTCAGGATATCTGAGAAGACA1690.11191978861067144No Hit
GTAATAGGGAAGAAACCTTTTAATAACTGAGTTTAGCAAAAAGAATAAAACT1690.11191978861067144No Hit
AAGCAGGGAGAAAACAAGATGAGATTGGCATGGCTTTATTTGTTTTTTTTGT1690.11191978861067144No Hit
CGCTGAGGGGTTGGGAGAGAGAAAGGAAGTCAAGTTGCTTAAATCATTTTAC1680.11125754134078583No Hit
CGTAAAGGGATTTCAACATAACCAACATTAGAACAAGTCTGCCATGTTAAAA1680.11125754134078583No Hit
GGCAATGGGCAGAAAACTTGAGTGTTTTTATTGCCATGTTAGCATATGAATC1680.11125754134078583No Hit
AATTGAGGGCAACTGGATTTCTTTTGACAATACTGTTGGTACCTATTACTTG1680.11125754134078583No Hit
AGCCATGGGATAATTTAAAGGAGCTGCTTTTTTATAGCACATACTATTTCGC1670.11059529407090019No Hit
TACGCTGGGCTCATTAAATTCTCCAAGAAAAAATAAGTTGAAGAATTTTATT1670.11059529407090019No Hit
CTAGTTGGGGAGAGGCTAAGGTGGAAGAATCACTTGAGCTTGGGCAGTTGAG1670.11059529407090019No Hit
CACATGGGGATAGTCCATTATTTGGTATTGTTTGTGTATGGGAGCAGACCAC1660.10993304680101455No Hit
TTTAAAGGGCACCGTGAATGAACATGTAGCTTCTGAAAAGGCTTCCTGTTGC1660.10993304680101455No Hit
GCACGAAGGGGTTTACCACAGGTCGGTTCTCCTGTCCCCGGGTTAATGGGAG1660.10993304680101455No Hit
CGGTTTGGGATAGGCACCCAGACAACTGCTGCTGCCTGCAATACTTCCTAAC1660.10993304680101455No Hit
ACCTAGGGGGCTCATCTTGCTGGTACCCTTCCGCTCCCTGAGCCCAAAGAGG1660.10993304680101455No Hit
CGTTGAGGGAGCCGATTTTCCGGAAAAAGGCTAAAACTACAAAGAAGATTGT1650.10927079953112893No Hit
GCAATGGGTAATGGGGGAGAAGTGACATTATAGTGACCTTTCGATTGTTAAC1650.10927079953112893No Hit
CATCCAGGGGAAACTTTATTGAAAGACATTAAAGAAGATTTAAATACATGGG1650.10927079953112893No Hit
CCCGGTGGGCAACGTCATTGAGGAGACTTTTATAAGAAAATGAAAAAAAGAA1640.1086085522612433No Hit
TAGAACGGGATTCTCCTCAGGGATGCCATTGAAAAGTTATTTCCTGATGCCA1640.1086085522612433No Hit
ATTCTTGGGGAGTAGGTAGAATTTTTGCTATGCTGTAATTTGTTGTATATTC1640.1086085522612433No Hit
CGCCAAGGGATTGCATTGCCTCTAATGATCATCACTTTCAGGGAACTAGGTG1640.1086085522612433No Hit
TTAGCATGGGGGTAGACACTTGTGTGCCTGGAGATTCTCTGGGGACCCTTCC1630.10794630499135767No Hit
GAAAAAGGGCATTACTGCTTTCTGTGAGGGCAGGATTCCTGTTTGTCCTGCT1620.10728405772147205No Hit
GAACATGGGATTGCTGGAGGGAATCTAAAATAGTACTGCTGCTTTCAAAAAT1620.10728405772147205No Hit
TCTGCCGGGCTCTCTGGACCTTGGTATTTATTCAACGAAAAATTATTCAACA1620.10728405772147205No Hit
AGCATGGGGATAATGGGTTGCTTAAAAATTGGTACCTTCCTATAAACTTGAT1620.10728405772147205No Hit
TTAAGCGGGGCTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGA1600.10595956318170077No Hit
TAAAGCGGGAAGCAGGCTGTCCTTTATAATGCATTGTCAGCCATGCTGGCGT1600.10595956318170077No Hit
ACAACTGGGAGTGTGGCCAAGAAAATAATCTTTGCTTTGCACCAGGTTGGAG1600.10595956318170077No Hit
GTAGAGGGGAAATCCTTTTCAGAAAAAATATAATTGCAAATAGGTAGGAGTG1600.10595956318170077No Hit
GTGGGTGGGCAAGTAGGGTATGATTTGAACTTTTCAAAAAATGTATGAGTCA1590.10529731591181517No Hit
CAATACGGGATATGAGGGGTTAATACCAATAGGATAGGAACAGTTTTTAGAC1590.10529731591181517No Hit
GACTGCGGGGAGGAAAGGAAAGTGGTAGTCTTTGCCTGCATTTCAGCATGCA1580.10463506864192952No Hit
CATTTAGGGAACCTGCCTGGACCTGCTACCACTGGTTTGTTTGTATGATGTC1580.10463506864192952No Hit
GGTAACGGGTATTTTAATGTTATTTTAAATTTTTTTGCTTTTGTAACATTAT1580.10463506864192952No Hit
GCAATTGGGAATGCAAAGTCCCTTTCTTACACTTCCATAAGCACTGGTAAGA1580.10463506864192952No Hit
ATTTGCGGGAAGTCTTAGGATAAACTCAAATCACAAAATATACAACAGGTAC1580.10463506864192952No Hit
TGCCAAGGGCAGTTTCTGAAGCTGAAAACTACCAGAATACTCTCCAGCTAGA1570.10397282137204389No Hit
TATTACGGGAATGATGAAATAATGAAAATGAAATGGAAATGAGGTGAAATGA1570.10397282137204389No Hit
AACGCCGGGGTGCCTTGAATAAAAATACTCAGTTAAATAAGTGTATTTCAGT1570.10397282137204389No Hit
CGTTGCGGGTCCGTATATCTTCTGTAATAGGCATTTATTCAAACCTTTTGCA1570.10397282137204389No Hit
AGATGAGGGATTGCAAGGTATAATATGGAGTAAGAAACACGAGAGAGGCTTC1570.10397282137204389No Hit
AACACAGGGTATTTAAAATTTGAGACTAATGAGATCTCAAAAATCAGCCCCA1570.10397282137204389No Hit
CTGTAAGGGATACAAAACAAGCCAGCATCTGATCAAAAGTATTACATAAAAT1570.10397282137204389No Hit
TCAGTTCGGGTATACGATGGCTTCATGTGTACAGGTAACAAATTTCATTAAT1570.10397282137204389No Hit
CGTCACGGGAATCATAAGTTAGGGAGAAAAGGGAAAGGAGTGGCAATATTAT1560.10331057410215827No Hit
CTCCTGGGGCCCTTGACTTTGCCGTCTCAAAGTTTCTTAGCTGACTTTGGCT1560.10331057410215827No Hit
TTCTGTGGGCATTCTTAAAAATATTATATCTTTATGGGGGAAAATAGTCTTA1560.10331057410215827No Hit
GCTCGCGGGCAATAACCATCAAGGATGATGTAATTACAAAACTCAAGATGAT1550.10264832683227264No Hit
CCTCTAGGGGGTAGACCAAAAGGAGGAAGCTGGGGAAAAAGAGGTGTGCGGA1550.10264832683227264No Hit
TTCCGGGGCATTGTGAAGACCAATGTCAAGAAGCTTTCCCTGCTCCATGTTT1550.10264832683227264No Hit
TTAGTAGAGGGCAGGGAGGTTCAAATTAGCCGGGTATTAGAAAAGTTTCCAT1540.101986079562387No Hit
GTACGCGGGAAAGATGATAAGAAGTAACCTTTTTTTTCCATAGCAGTAACCA1540.101986079562387No Hit
TCACTTGGGCTAAAAAGGAATTTTTGTATTATAAGGGAGGGGTAGGCAGAGA1540.101986079562387No Hit
TACCACGGGATGGGAGTTTGAGTCAAACCTGAGCAACATAGAGAGATACCAT1540.101986079562387No Hit
TGCGTAGGGACCCATAACAATATTCAGCACATAATAGGGATTTGATAAGTGT1540.101986079562387No Hit
AGTGGCGGGACTCGCAGCCCTTTGTCTGACTCATAGTGTAGGCTGCAGGCTC1540.101986079562387No Hit
GAAACAGGGCCGAGAGTTCATCTGCGCTCTGTCCATCACCTCCAGGGGCAGC1520.10066158502261574No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)