FastQCFastQC Report
Sat 31 Dec 2022
SRR5844732.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844732.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149899
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGGACTCATCTCGTATGCCGTCT6940.46297840545967617No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCAGGACTCATCTCGTATGC5820.3882614293624374No Hit
GAACATGGGGGTAGAAAAATAAAGCAAGGAAGAAGTAAAAAATGTGTATCTG2460.16411050107072095No Hit
GTGGACGGGGGTTTAGTGGAATAGAAATGCACAAGAACAATAGAGATCAGGT2350.1567722266325993No Hit
GCAGCCGGGATGTGAACAGACAGGAATTTCTTGTGCAATGTGGAGCAAATGG2310.15410376320055505No Hit
TGCTCAGGGAAAGAACTTCTGACTTTTGAGGAAAATCTAGCTTTCCAAGTAA2280.15210241562652185No Hit
TGCTTAGGGATGAAAACTTGAAATAATAAAAAGAGAAAAAAACTATTTCCTA2270.1514352997685108No Hit
ACAACACGGGATTTTTCTTGCTGGGGTTGAGGAGAGAAAATATAGAATTCCT2270.1514352997685108No Hit
TAATGTGGGTGCGTGCAAAGGCACGATTGATTTATCATGCTGGCTTTTATGC2230.14876683633646656No Hit
CATTATGGGAAGTTAGAAGAATTTTGAAGAAACAGAAAAGTAACTACATCTC2230.14876683633646656No Hit
CGCGCTGGGGGCGGGAGCCGGGGGGCGCAGCTAGAGAGCCCCGGAGCCGCGG2220.1480997204784555No Hit
CATAGCAGGATAGCTTATTTTTAAATTTAAAAAATCAGTTTTTAAAAGTATA2220.1480997204784555No Hit
ATGGTTGGGGAGCTCAAGTACATGAATGTTAGTTGTTATCACATACAGCAAA2140.142762793614367No Hit
TAACAAGGGGCTTGAAGAGACGCTAATTTTCTGTGGTTGAAGAGATGCTGTG2120.14142856189834488No Hit
GGCTCCGGGGATTCACCAGTCTGCCCTGTCACGTAGTTGAAGGAGTTGCATT2100.14009433018232276No Hit
AGAACCGGGATTGAGATCCTTTGCCCATTTTTGAATTGTGTTTTCTTTTTGT2100.14009433018232276No Hit
GAAGCGGGATATTATCTCATCTGATCTTTGTAATATAACTGTAACTTTCAAT2090.1394272143243117No Hit
GACTGCGGGGAGGAAAGGAAAGTGGTAGTCTTTGCCTGCATTTCAGCATGCA2090.1394272143243117No Hit
TGTCATGGGAGGTCAGCATGTGAAATGTTGGATTTGACATGTCAGGTAGGGT2080.13876009846630064No Hit
ACTGCCGGTCCTGTCCAGCAGGGGTCTCCCAACAAGTGCTCTTTCCACCCCA2070.1380929826082896No Hit
TTCAAGGGGAGTGGTATTAATTTGCAAGTAGGGAAAAGATACAGACAAGATT2060.1374258667502785No Hit
AAGTACGGGAGGGTACTTTTATTGATGGATATGTTTTCCTTTTATTCACATA2040.1360916350342564No Hit
GGGTATGGGATTGCTACTCATGGAAACTAGGGTTCACAGATTACTTCCAAAA2020.13475740331823427No Hit
GAACACGGGGCTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGA2010.13409028746022322No Hit
TGCCAAGGGCAGTTTCTGAAGCTGAAAACTACCAGAATACTCTCCAGCTAGA2010.13409028746022322No Hit
CATTTAGGGAACCTGCCTGGACCTGCTACCACTGGTTTGTTTGTATGATGTC2010.13409028746022322No Hit
ATAGCAGGGGTATGGATGTCTACAAAGGGATGTGCTGTAACTCAAGTATAAG2010.13409028746022322No Hit
ATGTCTGGGCTATGTCTTCTTTGTTCTCCTTTTCTTCTTTTTCTGCTTTCCC2000.13342317160221215No Hit
ATTGCAGGGAGGGCCCCGGGGGCTGCGGAGGTGGCTGAGACGCGGCTGGCTG2000.13342317160221215No Hit
TATTCTGGGGTTCCTGTGGACATCAGGAATGGCCATGGAACTTGATTTGGCC1990.1327560557442011No Hit
TGACAAGGGAACATATAACTTTAAGAAGTGAAGAAAATAATTGAAGTTAGAA1990.1327560557442011No Hit
CATGCTGGGAAGCTTTTTTGATGGTTGTTAATTTTTTGATGGGGAGTATCAA1970.13142182402817898No Hit
TCTGCTAGGGGTGAAACAACATATTTGAAGTTCTGGAAAAAGAATAAATCAA1960.1307547081701679No Hit
ACTTCCGGGGTTGGCATATATGAAATGTAAACATATCTCTTCCCATCCAGGG1960.1307547081701679No Hit
TTCTGTGGGCATTCTTAAAAATATTATATCTTTATGGGGGAAAATAGTCTTA1960.1307547081701679No Hit
AGATTAGGGACTGAATTGAGAGATATGCTCCATGTTTAAAAATATTTGTAAT1950.13008759231215686No Hit
CGGTTCGGGACATTTTATAATTTGGAGGACTTGAGCATCAGTGGATTTTGGT1940.12942047645414578No Hit
CCAAACCGGGCTGTTTAGAAAATGCCATCACAAAGGGGACAGCACATGCAAA1930.12875336059613474No Hit
TAGCAGGGGATCCAGTACTTGTGGACACTACACGTTTCAACCTCTCTACTAG1920.12808624473812366No Hit
CTCAAGAGGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAG1900.12675201302210154No Hit
GCGCTAGGGAAGGACATTTTCTACATTGAAAACCAAAAGGAATATGAAAATA1900.12675201302210154No Hit
AAGGGAGGGCTGCCCAGCCAGCTCAGGCCTCACTGAGGGCCTGGGTGACTAC1890.1260848971640905No Hit
TCAGTAGGGAATAGAAATACTCGGAGACTAACTTGTGAATAACTGCTTACAA1890.1260848971640905No Hit
CCCGGTGGGCAACGTCATTGAGGAGACTTTTATAAGAAAATGAAAAAAAGAA1880.12541778130607942No Hit
CGGTTAGGGATCTCTAGATTTTTTATAATACTTAATACAATGTAAATGCTAT1880.12541778130607942No Hit
ACGATGGGGGCGGGGAGAGGAGTACGGGTTCAGCGTGGACTGGTGGGCGCTG1880.12541778130607942No Hit
TCAGACAGGGATTGGGGAAATATCTGAGAAGAGAGGTCTTGTTTTATCCAAC1870.12475066544806836No Hit
CATAACGGGAACCTAGAGAGATTTTGAGCCTCATATTTCTTTGATACTTGAA1870.12475066544806836No Hit
CGGTTTGGGATAGGCACCCAGACAACTGCTGCTGCCTGCAATACTTCCTAAC1860.1240835495900573No Hit
GAAAAAGGGCATTACTGCTTTCTGTGAGGGCAGGATTCCTGTTTGTCCTGCT1850.12341643373204624No Hit
AAACAGGGGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTG1850.12341643373204624No Hit
TTGCATGGGTTACTAGGCAGAATTCACTAATGGAGTAGATATGTGGCATTCA1850.12341643373204624No Hit
CAGCTAGGAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCTAAAAC1840.12274931787403517No Hit
ATTCTTGGGGAGTAGGTAGAATTTTTGCTATGCTGTAATTTGTTGTATATTC1840.12274931787403517No Hit
TGTGTAGGGATGATTATACAGTGACACAATTTTCAGAACCTACTCTTGTCAT1840.12274931787403517No Hit
TCACTTGGGCTAAAAAGGAATTTTTGTATTATAAGGGAGGGGTAGGCAGAGA1830.12208220201602411No Hit
TCTCAAGGGATATGCTGTTTTGTAGAGTGATGACAGAGAAACAGATACAGCA1830.12208220201602411No Hit
GACGCAGGGTATGTGTCAATCGACTATGTTATCCGTAAGACTTCTGGTCAGC1830.12208220201602411No Hit
GGTTCCGGGCAAAGAAAAATGGTGTGCTTTAGGGAGGGAAAAGACAGATTTC1820.12141508615801305No Hit
TACGTTGGGCAAAAATCTTACGATGATAATAGTGAGCACCTTATTGCTAAGT1810.12074797030000199No Hit
ACAGCAGGGATTCTATTTATTGAGAAAGTGATTTAAAATTATTATTTTTAAT1810.12074797030000199No Hit
TGCACATGGGACTGAGCATGTAGAATTTATTTATTCTAATTATAGGATTCTG1810.12074797030000199No Hit
GCTTTGGGGGGTAACGGGGCAGAGAGGCTGTTCGCAGAGCTGCGGAAGATGA1810.12074797030000199No Hit
TAGAACGGGATTCTCCTCAGGGATGCCATTGAAAAGTTATTTCCTGATGCCA1800.12008085444199093No Hit
ACCCCCAGGTCCCATGAGAACTCATTCATACAGTTCTCTGCTAATTGATTCA1790.11941373858397987No Hit
TAGATAGGGAAGAATATCTTGAATGTTGTGTATATATTTTAAAAAGCACTTT1780.11874662272596881No Hit
CGCCAAGGGATTGCATTGCCTCTAATGATCATCACTTTCAGGGAACTAGGTG1780.11874662272596881No Hit
TTAGACGGGATCTAGCAAAAATATTCTTTGTAACTGAAGGAGAAATAAAGTC1770.11807950686795775No Hit
CCTCTAGGGGGTAGACCAAAAGGAGGAAGCTGGGGAAAAAGAGGTGTGCGGA1760.11741239100994669No Hit
GAAATAGGGAGGTAGACATGAACTTTTCCTGCACAAAGCCTTGCTTTTAGAG1760.11741239100994669No Hit
TACTTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1750.11674527515193563No Hit
TAAAGCGGGAAGCAGGCTGTCCTTTATAATGCATTGTCAGCCATGCTGGCGT1750.11674527515193563No Hit
TACCTGGGGTAACACACAATGGGAAAGTATTGTCTTTTCAACAAATAGTGTT1750.11674527515193563No Hit
AAATATGGGAATGTTTCAGCATTATGATTTTCAAGACTTTAGGGATTTTGAT1750.11674527515193563No Hit
AAGTATGGGAGGCCGACCAGCACTTGGGATCTGATGGGACACGGCCAGTGCC1730.1154110434359135No Hit
TTTAAAGGGCACCGTGAATGAACATGTAGCTTCTGAAAAGGCTTCCTGTTGC1730.1154110434359135No Hit
CGTTGCGGGTCCGTATATCTTCTGTAATAGGCATTTATTCAAACCTTTTGCA1730.1154110434359135No Hit
AGTAGCGGGTCCGATCTCAAAGAGGGCCTCTGACACATTCATGATTGATTGA1730.1154110434359135No Hit
AATTGAGGGCAACTGGATTTCTTTTGACAATACTGTTGGTACCTATTACTTG1730.1154110434359135No Hit
TAAAGGGGGACAGCTTGCCTTTGCTTTTTCTCACACACATTCCTAGCCTGAA1730.1154110434359135No Hit
GCCACGGGACATGGAAGTGGCTTTAAATCAAGCACATTAACCTCAATGAACT1720.11474392757790244No Hit
TTGGTAGGGTATCCAGGCTCCATGACAGTCTTGCCATAGAAAGAAAAATAAA1720.11474392757790244No Hit
AATTGTGGGAGGAGTTCATTAGCCAACTAAGAAGAACCTACATCATTTGCAG1710.11407681171989138No Hit
CGTAAAGGGATTTCAACATAACCAACATTAGAACAAGTCTGCCATGTTAAAA1710.11407681171989138No Hit
ATTGTAGGGATGATAAAGCTGTTGCTACATCACAGCTTCAGAAAAAACTTGG1710.11407681171989138No Hit
AGCCATGGGATAATTTAAAGGAGCTGCTTTTTTATAGCACATACTATTTCGC1700.11340969586188032No Hit
CCCATGGGATGGAGACCCGAAGGGACTCAGACGGAGCCGCCGTGTTGGCAGC1700.11340969586188032No Hit
AATTTGGGCAGCTCAGTATCATCCCATCTCCCTCAGGATATCTGAGAAGACA1700.11340969586188032No Hit
AACTCAGGGCCGTCTCAAAGAAAAAAAATTGTTTCCTTGCTGTGATTTTGGT1700.11340969586188032No Hit
TCAGTTCGGGTATACGATGGCTTCATGTGTACAGGTAACAAATTTCATTAAT1700.11340969586188032No Hit
CACACCGGGGAGAGGAGAATTGTTAGTAACATCAAAGGTCAGAATGCTTCAG1700.11340969586188032No Hit
TATGCAGGGACTTCTGACACTGTGTTCCCTCTTGGAACCTGACCGTCAATGC1700.11340969586188032No Hit
GCACAAGGGCAAACTCATAAACCAGCAAAGAGAAAAGTAATAAAAACTGTAC1690.11274258000386926No Hit
CATCCAGGGGAAACTTTATTGAAAGACATTAAAGAAGATTTAAATACATGGG1690.11274258000386926No Hit
ATTATTGGGAGCGCCCAGTAACTCATCCTTCATTCAGAGGTTTGCTGGCCTT1680.1120754641458582No Hit
ACTGATGGGAGGCATTGAAAATAGGTGGCCTGACTGATTTAAACATTCTTTT1680.1120754641458582No Hit
GCAATGGGTAATGGGGGAGAAGTGACATTATAGTGACCTTTCGATTGTTAAC1670.11140834828784714No Hit
GGTAACGGGTATTTTAATGTTATTTTAAATTTTTTTGCTTTTGTAACATTAT1670.11140834828784714No Hit
TTGCGCGGGGCAGTTCAATATAGCTAATTTTTTATTCTTCTGCTCATGAAAA1660.11074123242983608No Hit
TTACATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1650.11007411657182502No Hit
AAACTGGGGGGCTCTCTCCAGCAGTGACAACTCAGCATCTCTCTAGTGGCCA1650.11007411657182502No Hit
ACTAGGGGTGAGACTCCTTTTAAGACTAAACCATTTGCATTAAACTAGAGCC1650.11007411657182502No Hit
CTTCTTGGGAAGATCAAGGCTCCTGGTTTTGCCCATCTGGCTGGTTTGGACA1640.10940700071381396No Hit
CTGCATGGGAAGTACAAATGCATCCCATAAGACAAGATTTCAAAAGACTCAG1630.1087398848558029No Hit
AGCATGGGGATAATGGGTTGCTTAAAAATTGGTACCTTCCTATAAACTTGAT1630.1087398848558029No Hit
ATTGAAGGGCAAGCCAAGACCAAGTTAGGTTTATGTGTTTTTGGCTTAACCT1630.1087398848558029No Hit
GCATTAGGGAAGTGAAGTTAAAGGACAGCAGACAGTAACTCAAAGCTGTATG1630.1087398848558029No Hit
TTTAAAGGGATTGGTGAAATCACATATTTTATATTCAGCATAAAGGAGAATA1620.10807276899779183No Hit
CGCTGAGGGGTTGGGAGAGAGAAAGGAAGTCAAGTTGCTTAAATCATTTTAC1600.10673853728176971No Hit
TGCGTAGGGACCCATAACAATATTCAGCACATAATAGGGATTTGATAAGTGT1600.10673853728176971No Hit
AGACTAGGGTAGATATGGGTTCAAGTTCAGTCTTTGCCATTTATTGGCTACT1590.10607142142375865No Hit
CACATGGGGATAGTCCATTATTTGGTATTGTTTGTGTATGGGAGCAGACCAC1590.10607142142375865No Hit
GGTCTGGGGACATGCAACCATGCCTAGCTAATTTTTTCTTTTCTTTTCTTTT1590.10607142142375865No Hit
CGGGTCGGGTATAAATAAGGAATTTTTATTTTTTCAAGAGCTGGTTTATAGG1590.10607142142375865No Hit
GTTTAAGGCGATCTGACACAGGGGAATAGTGTGTATCAATAAAAAATAACAA1590.10607142142375865No Hit
GGTTACGGGAACTCATATAAAAATTATATTTAGAATTTTTAAACATGTACAA1580.10540430556574759No Hit
TTAAGCGGGGCTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGA1580.10540430556574759No Hit
GTGTGTGGGAGTATCGTCTGCATTCCAGCAAAAATATCAACTCGTAAGGCAC1580.10540430556574759No Hit
AACATAGGGAAATGCAAGAGGGAAGTTGTTTTACTGTTAACTGATTATAGAT1580.10540430556574759No Hit
AACACAGGGTATTTAAAATTTGAGACTAATGAGATCTCAAAAATCAGCCCCA1580.10540430556574759No Hit
TGCTGGGCAGAAAACTTATTACCCTTCTCCACCAAGGTAACCAATGAATGTT1580.10540430556574759No Hit
TGTTATGGGGATGTTGACAGGGATAAATGAATAGGAAAAGCATGCAGAGAGT1580.10540430556574759No Hit
TGCTATGGGCTTGGAATAAAATGTGTTTCATAACTTAGCTATATTAAATACA1580.10540430556574759No Hit
CGTTGAGGGAGCCGATTTTCCGGAAAAAGGCTAAAACTACAAAGAAGATTGT1570.10473718970773653No Hit
AGCAAAGGGATGACTGGAAAATAAAGTGTGCCAGACGTAAGGAATGATTCTA1570.10473718970773653No Hit
CTCTTGGGGTCTGAGCTTTTACCACTAGACTCAAGGAAGAATAATTTTAACA1560.10407007384972547No Hit
TCTGCCGGGCTCTCTGGACCTTGGTATTTATTCAACGAAAAATTATTCAACA1560.10407007384972547No Hit
TATGCTGGGGTACAGTTGGTACTAGAAAGTGTACAATTGGGAATGGTCTATG1560.10407007384972547No Hit
TCAAACGGGAACCAGGGATCAGGAGGGTCCAAAGCCGACATTCCCAGTCCTG1560.10407007384972547No Hit
ATCTTTAGGGAGGGAAAATCAGACAAAACAAAAGAAACAAACCAAAATATCC1550.10340295799171441No Hit
TTCAATGGGAAGGTCTCTGAGAGTAGAAATCATGTCTAATCTTTGTATCCTA1550.10340295799171441No Hit
GCACGAAGGGGTTTACCACAGGTCGGTTCTCCTGTCCCCGGGTTAATGGGAG1550.10340295799171441No Hit
GTACGCGGGAAAGATGATAAGAAGTAACCTTTTTTTTCCATAGCAGTAACCA1550.10340295799171441No Hit
GCAATTGGGAATGCAAAGTCCCTTTCTTACACTTCCATAAGCACTGGTAAGA1550.10340295799171441No Hit
CTAGTTGGGGAGAGGCTAAGGTGGAAGAATCACTTGAGCTTGGGCAGTTGAG1550.10340295799171441No Hit
CTGCATAGGGCTTATACATTCAATTTAAAAAAAAGATCCAAACTTCTTATCA1540.10273584213370335No Hit
GTGGTTGGGATGGACGAGCGAGATGTGAGGCGATTTCAACTAAAAATTGCTG1540.10273584213370335No Hit
GTCGCGGGGGACGGCGGGCTGAATACACCCAATAGCAAGCACCAGGTGGAGG1530.10206872627569229No Hit
ATCATTGGGAGAGAGGGAGGGAGGGAGAGAGAGCGCGCTAGCGCGAGAGAGC1530.10206872627569229No Hit
TACGCTGGGCTCATTAAATTCTCCAAGAAAAAATAAGTTGAAGAATTTTATT1530.10206872627569229No Hit
AAGCAGGGAGAAAACAAGATGAGATTGGCATGGCTTTATTTGTTTTTTTTGT1530.10206872627569229No Hit
CGTCACGGGAATCATAAGTTAGGGAGAAAAGGGAAAGGAGTGGCAATATTAT1520.10140161041768123No Hit
TGTTAGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1520.10140161041768123No Hit
GACATAGGGAGCTCCTCTGAGTGGGGAGAGGTTGGGCAGTGAGTGAGGGACC1520.10140161041768123No Hit
AGTCAGGGCATATAAGTTCATATTCTCTTGGGTCCTGGTTGGAAAATTAGGC1520.10140161041768123No Hit
GAAACAGGGCCGAGAGTTCATCTGCGCTCTGTCCATCACCTCCAGGGGCAGC1520.10140161041768123No Hit
ATGCTAGGGCATATGAAACCAGTGTGTTATTATTTTTCCCCCTTTCCCTTAT1520.10140161041768123No Hit
TTAGCATGGGGGTAGACACTTGTGTGCCTGGAGATTCTCTGGGGACCCTTCC1510.10073449455967017No Hit
AAATTGGGGTAATTCATATACCATCAATTCACCCTTTTAAAGTGTACAATTT1510.10073449455967017No Hit
CAGAACGGGTCTAACCAAGAGCCACATGCTTACCATGTATCAACCAGTAGCT1510.10073449455967017No Hit
TATTACGGGAATGATGAAATAATGAAAATGAAATGGAAATGAGGTGAAATGA1500.1000673787016591No Hit
GTAAGCGGCGAACTCCTATGCTCAAGTGCCTCCTGCCTTCACTTCTCAAGGT1500.1000673787016591No Hit
TCCGTCTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG1500.1000673787016591No Hit
TTTTATGGGTGTTACCAGAGTTATCAGGTAGTCATTAGGTTTCATACAGATG1500.1000673787016591No Hit
TACCATGGGAGTACAGACCCTAAACTTGTCATTTGTTTTTCTCACAGAATAG1500.1000673787016591No Hit
AAACTGGGGCTTCTGTAACTTGTTTGGGTCATATCAGTGGTGGTTCAAGTAG1500.1000673787016591No Hit
ATCCCAGGGGAGAAGAAAACACATGCCTGTCACTCTACGGCAGCTGCCACCA1500.1000673787016591No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)