FastQCFastQC Report
Sat 31 Dec 2022
SRR5844731.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844731.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149945
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGGACTCATCTCGTATGCCGTCT6080.4054820100703591No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCAGGACTCATCTCGTATGC5770.38480776284637697No Hit
TGCTTAGGGATGAAAACTTGAAATAATAAAAAGAGAAAAAAACTATTTCCTA2690.17939911300810296No Hit
AGTAGCGGGTCCGATCTCAAAGAGGGCCTCTGACACATTCATGATTGATTGA2390.15939177698489446No Hit
TAATGTGGGTGCGTGCAAAGGCACGATTGATTTATCATGCTGGCTTTTATGC2380.15872486578412084No Hit
ACAACACGGGATTTTTCTTGCTGGGGTTGAGGAGAGAAAATATAGAATTCCT2350.1567241321818No Hit
GAACATGGGGGTAGAAAAATAAAGCAAGGAAGAAGTAAAAAATGTGTATCTG2260.15072193137483744No Hit
TAACAAGGGGCTTGAAGAGACGCTAATTTTCTGTGGTTGAAGAGATGCTGTG2220.14805428657174297No Hit
GAACACGGGGCTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGA2190.14605355296942213No Hit
TTGGTAGGGTATCCAGGCTCCATGACAGTCTTGCCATAGAAAGAAAAATAAA2180.14538664176864852No Hit
TTGCATGGGTTACTAGGCAGAATTCACTAATGGAGTAGATATGTGGCATTCA2170.1447197305678749No Hit
CATAGCAGGATAGCTTATTTTTAAATTTAAAAAATCAGTTTTTAAAAGTATA2140.14271899696555404No Hit
GAAGCGGGATATTATCTCATCTGATCTTTGTAATATAACTGTAACTTTCAAT2090.13938444096168595No Hit
AGATTAGGGACTGAATTGAGAGATATGCTCCATGTTTAAAAATATTTGTAAT2090.13938444096168595No Hit
GTGGACGGGGGTTTAGTGGAATAGAAATGCACAAGAACAATAGAGATCAGGT2090.13938444096168595No Hit
TGCTCAGGGAAAGAACTTCTGACTTTTGAGGAAAATCTAGCTTTCCAAGTAA2090.13938444096168595No Hit
TAGATAGGGAAGAATATCTTGAATGTTGTGTATATATTTTAAAAAGCACTTT2080.13871752976091234No Hit
ACTTCCGGGGTTGGCATATATGAAATGTAAACATATCTCTTCCCATCCAGGG2070.13805061856013873No Hit
GGCTCCGGGGATTCACCAGTCTGCCCTGTCACGTAGTTGAAGGAGTTGCATT2070.13805061856013873No Hit
ACAGCAGGGATTCTATTTATTGAGAAAGTGATTTAAAATTATTATTTTTAAT2020.13471606255627064No Hit
TGTCATGGGAGGTCAGCATGTGAAATGTTGGATTTGACATGTCAGGTAGGGT2000.13338224015472339No Hit
GAAAAAGGGCATTACTGCTTTCTGTGAGGGCAGGATTCCTGTTTGTCCTGCT1990.13271532895394977No Hit
TGACAAGGGAACATATAACTTTAAGAAGTGAAGAAAATAATTGAAGTTAGAA1990.13271532895394977No Hit
AGAACCGGGATTGAGATCCTTTGCCCATTTTTGAATTGTGTTTTCTTTTTGT1960.13071459535162894No Hit
CATTTAGGGAACCTGCCTGGACCTGCTACCACTGGTTTGTTTGTATGATGTC1950.13004768415085532No Hit
GACGCAGGGTATGTGTCAATCGACTATGTTATCCGTAAGACTTCTGGTCAGC1950.13004768415085532No Hit
GCGCTAGGGAAGGACATTTTCTACATTGAAAACCAAAAGGAATATGAAAATA1940.12938077295008168No Hit
TCAGACAGGGATTGGGGAAATATCTGAGAAGAGAGGTCTTGTTTTATCCAAC1930.12871386174930807No Hit
AATTGAGGGCAACTGGATTTCTTTTGACAATACTGTTGGTACCTATTACTTG1910.12738003934776085No Hit
TTCAAGGGGAGTGGTATTAATTTGCAAGTAGGGAAAAGATACAGACAAGATT1910.12738003934776085No Hit
ATGTCTGGGCTATGTCTTCTTTGTTCTCCTTTTCTTCTTTTTCTGCTTTCCC1900.12671312814698724No Hit
CCAGCCTGGACGACAGAGCGAGACTCTCTCAAAACACATACAAACAAAATTA1890.12604621694621362No Hit
TCTGCTAGGGGTGAAACAACATATTTGAAGTTCTGGAAAAAGAATAAATCAA1890.12604621694621362No Hit
TCTGCCGGGCTCTCTGGACCTTGGTATTTATTCAACGAAAAATTATTCAACA1870.12471239454466639No Hit
TAAAGGGGGACAGCTTGCCTTTGCTTTTTCTCACACACATTCCTAGCCTGAA1870.12471239454466639No Hit
AAATATGGGAATGTTTCAGCATTATGATTTTCAAGACTTTAGGGATTTTGAT1870.12471239454466639No Hit
GCAGCCGGGATGTGAACAGACAGGAATTTCTTGTGCAATGTGGAGCAAATGG1860.12404548334389277No Hit
CACATGGGGATAGTCCATTATTTGGTATTGTTTGTGTATGGGAGCAGACCAC1850.12337857214311915No Hit
CGGTTCGGGACATTTTATAATTTGGAGGACTTGAGCATCAGTGGATTTTGGT1850.12337857214311915No Hit
AAGTACGGGAGGGTACTTTTATTGATGGATATGTTTTCCTTTTATTCACATA1850.12337857214311915No Hit
CATGCTGGGAAGCTTTTTTGATGGTTGTTAATTTTTTGATGGGGAGTATCAA1850.12337857214311915No Hit
GAAATAGGGAGGTAGACATGAACTTTTCCTGCACAAAGCCTTGCTTTTAGAG1850.12337857214311915No Hit
ATTCTTGGGGAGTAGGTAGAATTTTTGCTATGCTGTAATTTGTTGTATATTC1850.12337857214311915No Hit
GCAATTGGGAATGCAAAGTCCCTTTCTTACACTTCCATAAGCACTGGTAAGA1840.12271166094234552No Hit
GACTGCGGGGAGGAAAGGAAAGTGGTAGTCTTTGCCTGCATTTCAGCATGCA1830.1220447497415719No Hit
CACACCGGGGAGAGGAGAATTGTTAGTAACATCAAAGGTCAGAATGCTTCAG1830.1220447497415719No Hit
TATGCAGGGACTTCTGACACTGTGTTCCCTCTTGGAACCTGACCGTCAATGC1830.1220447497415719No Hit
CGTAAAGGGATTTCAACATAACCAACATTAGAACAAGTCTGCCATGTTAAAA1820.12137783854079828No Hit
CTGTAAGGGATACAAAACAAGCCAGCATCTGATCAAAAGTATTACATAAAAT1820.12137783854079828No Hit
TTCTGTGGGCATTCTTAAAAATATTATATCTTTATGGGGGAAAATAGTCTTA1820.12137783854079828No Hit
ATAGCAGGGGTATGGATGTCTACAAAGGGATGTGCTGTAACTCAAGTATAAG1820.12137783854079828No Hit
CGCTGAGGGGTTGGGAGAGAGAAAGGAAGTCAAGTTGCTTAAATCATTTTAC1810.12071092734002467No Hit
TATTCTGGGGTTCCTGTGGACATCAGGAATGGCCATGGAACTTGATTTGGCC1810.12071092734002467No Hit
CATAACGGGAACCTAGAGAGATTTTGAGCCTCATATTTCTTTGATACTTGAA1810.12071092734002467No Hit
CGCCAAGGGATTGCATTGCCTCTAATGATCATCACTTTCAGGGAACTAGGTG1810.12071092734002467No Hit
TGCACATGGGACTGAGCATGTAGAATTTATTTATTCTAATTATAGGATTCTG1810.12071092734002467No Hit
AACTCAGGGCCGTCTCAAAGAAAAAAAATTGTTTCCTTGCTGTGATTTTGGT1800.12004401613925106No Hit
CTTCTTGGGAAGATCAAGGCTCCTGGTTTTGCCCATCTGGCTGGTTTGGACA1790.11937710493847745No Hit
CGTCACGGGAATCATAAGTTAGGGAGAAAAGGGAAAGGAGTGGCAATATTAT1770.11804328253693021No Hit
CTCAAGAGGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAG1770.11804328253693021No Hit
GTTTGAGGGGAGGCGTGAGCCACCATGCCCGGCCCAAAGACAACTATTAATC1770.11804328253693021No Hit
TACCATGGGAGTACAGACCCTAAACTTGTCATTTGTTTTTCTCACAGAATAG1770.11804328253693021No Hit
AATTGTGGGAGGAGTTCATTAGCCAACTAAGAAGAACCTACATCATTTGCAG1770.11804328253693021No Hit
GCACGAAGGGGTTTACCACAGGTCGGTTCTCCTGTCCCCGGGTTAATGGGAG1770.11804328253693021No Hit
AATTTGGGCAGCTCAGTATCATCCCATCTCCCTCAGGATATCTGAGAAGACA1770.11804328253693021No Hit
ACTGCCGGTCCTGTCCAGCAGGGGTCTCCCAACAAGTGCTCTTTCCACCCCA1770.11804328253693021No Hit
GGTCTGGGGACATGCAACCATGCCTAGCTAATTTTTTCTTTTCTTTTCTTTT1760.1173763713361566No Hit
AACACAGGGTATTTAAAATTTGAGACTAATGAGATCTCAAAAATCAGCCCCA1760.1173763713361566No Hit
GCATTAGGGAAGTGAAGTTAAAGGACAGCAGACAGTAACTCAAAGCTGTATG1760.1173763713361566No Hit
ATCTTTAGGGAGGGAAAATCAGACAAAACAAAAGAAACAAACCAAAATATCC1750.11670946013538298No Hit
GGTTCCGGGCAAAGAAAAATGGTGTGCTTTAGGGAGGGAAAAGACAGATTTC1750.11670946013538298No Hit
AGCCATGGGATAATTTAAAGGAGCTGCTTTTTTATAGCACATACTATTTCGC1750.11670946013538298No Hit
GGTTACGGGAACTCATATAAAAATTATATTTAGAATTTTTAAACATGTACAA1750.11670946013538298No Hit
TGCCAAGGGCAGTTTCTGAAGCTGAAAACTACCAGAATACTCTCCAGCTAGA1740.11604254893460934No Hit
CATTATGGGAAGTTAGAAGAATTTTGAAGAAACAGAAAAGTAACTACATCTC1730.11537563773383573No Hit
CTCCTGGGGCCCTTGACTTTGCCGTCTCAAAGTTTCTTAGCTGACTTTGGCT1720.11470872653306212No Hit
CGCTGAGGGCCCCCGGCGCGCGGCCCCGCCCAGGACGAGGGGCGGTGGGCAG1720.11470872653306212No Hit
GACATGGGGTGACACTCTTACTAACTCTGTTTTCACAAACCCTTAATGGAGC1720.11470872653306212No Hit
AAATCAGGGGTATTCTTACCTCTTAGAATCTAGCTTGGCATTTTTCACGCTT1710.11404181533228851No Hit
TAGATGGGGAACTGTTGTGATCACAGTGCATTGTAGCCTGGAATTTCTGGGC1710.11404181533228851No Hit
TGTGTAGGGATGATTATACAGTGACACAATTTTCAGAACCTACTCTTGTCAT1710.11404181533228851No Hit
CCAAACCGGGCTGTTTAGAAAATGCCATCACAAAGGGGACAGCACATGCAAA1700.11337490413151488No Hit
AGCAAAGGGATGACTGGAAAATAAAGTGTGCCAGACGTAAGGAATGATTCTA1700.11337490413151488No Hit
AACATAGGGAAATGCAAGAGGGAAGTTGTTTTACTGTTAACTGATTATAGAT1690.11270799293074127No Hit
ACATCCGGGAGCTGTGTTTTTTTTAAAATTTTAAATGCAACTTGAGGAGTAT1690.11270799293074127No Hit
GTTTAAGGCGATCTGACACAGGGGAATAGTGTGTATCAATAAAAAATAACAA1680.11204108172996766No Hit
TCAAACGGGAACCAGGGATCAGGAGGGTCCAAAGCCGACATTCCCAGTCCTG1680.11204108172996766No Hit
TTTAAAGGGCACCGTGAATGAACATGTAGCTTCTGAAAAGGCTTCCTGTTGC1680.11204108172996766No Hit
ACTTTAGGGAGGAATCAAAACCTTCTTCACAAGTCCCTGCACCGAAGAGCCT1680.11204108172996766No Hit
GGTAGGGATTTAATAGATTGTTATCTTCTTCCAGATTACATCAATCTTCCAG1670.11137417052919404No Hit
GCCACGGGACATGGAAGTGGCTTTAAATCAAGCACATTAACCTCAATGAACT1670.11137417052919404No Hit
GCTTTGGGGGGTAACGGGGCAGAGAGGCTGTTCGCAGAGCTGCGGAAGATGA1670.11137417052919404No Hit
GGCCGGGGGTATTTCACAGCGATGGCTAAGGATTTCCCTCCATGACCTCCCC1670.11137417052919404No Hit
TCAGTAGGGAATAGAAATACTCGGAGACTAACTTGTGAATAACTGCTTACAA1670.11137417052919404No Hit
GGTAACGGGTATTTTAATGTTATTTTAAATTTTTTTGCTTTTGTAACATTAT1670.11137417052919404No Hit
TAAAGCGGGAAGCAGGCTGTCCTTTATAATGCATTGTCAGCCATGCTGGCGT1670.11137417052919404No Hit
CGTGCTGGGATTACAGGCGTGAGCCACCATGCCCGGCCCATAAGTACCGTTT1670.11137417052919404No Hit
ATTGTAGGGATGATAAAGCTGTTGCTACATCACAGCTTCAGAAAAAACTTGG1660.11070725932842043No Hit
CGCGCTGGGGGCGGGAGCCGGGGGGCGCAGCTAGAGAGCCCCGGAGCCGCGG1660.11070725932842043No Hit
GGGTATGGGATTGCTACTCATGGAAACTAGGGTTCACAGATTACTTCCAAAA1660.11070725932842043No Hit
GTAATAGGGAAGAAACCTTTTAATAACTGAGTTTAGCAAAAAGAATAAAACT1660.11070725932842043No Hit
ACTAGGGGTGAGACTCCTTTTAAGACTAAACCATTTGCATTAAACTAGAGCC1660.11070725932842043No Hit
CGCCGGGGGTCTGCTTCCCCCTTTAGAGAAAACAGTATTTTTATATTTTGTT1650.11004034812764682No Hit
GAACATGGGATTGCTGGAGGGAATCTAAAATAGTACTGCTGCTTTCAAAAAT1650.11004034812764682No Hit
AAACAGGGGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTG1650.11004034812764682No Hit
TTACAAGGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGG1640.10937343692687318No Hit
TGCTGGGCAGAAAACTTATTACCCTTCTCCACCAAGGTAACCAATGAATGTT1640.10937343692687318No Hit
ATTATTGGGAGCGCCCAGTAACTCATCCTTCATTCAGAGGTTTGCTGGCCTT1640.10937343692687318No Hit
TGCTATGGGCTTGGAATAAAATGTGTTTCATAACTTAGCTATATTAAATACA1640.10937343692687318No Hit
GAATTAGGGAGAGTCCACCATAAGATTTAACAGTTGACTTCTGGTAAAAACC1630.10870652572609957No Hit
CTCTTGGGGTCTGAGCTTTTACCACTAGACTCAAGGAAGAATAATTTTAACA1630.10870652572609957No Hit
TGCGTAGGGACCCATAACAATATTCAGCACATAATAGGGATTTGATAAGTGT1630.10870652572609957No Hit
TATTACGGGAATGATGAAATAATGAAAATGAAATGGAAATGAGGTGAAATGA1630.10870652572609957No Hit
AAGGGAGGGCTGCCCAGCCAGCTCAGGCCTCACTGAGGGCCTGGGTGACTAC1630.10870652572609957No Hit
TAGCAGGGGATCCAGTACTTGTGGACACTACACGTTTCAACCTCTCTACTAG1630.10870652572609957No Hit
TCACTTGGGCTAAAAAGGAATTTTTGTATTATAAGGGAGGGGTAGGCAGAGA1630.10870652572609957No Hit
TTGCGCGGGGCAGTTCAATATAGCTAATTTTTTATTCTTCTGCTCATGAAAA1620.10803961452532594No Hit
AGATGAGGGATTGCAAGGTATAATATGGAGTAAGAAACACGAGAGAGGCTTC1620.10803961452532594No Hit
TTACGGGAGACCCAGAAGAGAGACAACAATCACTGCAGTTTGGCTCACAGAA1620.10803961452532594No Hit
GCTCGCGGGCAATAACCATCAAGGATGATGTAATTACAAAACTCAAGATGAT1610.10737270332455233No Hit
CCCGGTGGGCAACGTCATTGAGGAGACTTTTATAAGAAAATGAAAAAAAGAA1610.10737270332455233No Hit
CCTTATGGACACATGTGAGATTTGCTGCTGTTGCAGTATAAACACTAGGTAT1600.10670579212377872No Hit
CAGAACGGGTCTAACCAAGAGCCACATGCTTACCATGTATCAACCAGTAGCT1600.10670579212377872No Hit
CGGTTAGGGATCTCTAGATTTTTTATAATACTTAATACAATGTAAATGCTAT1590.1060388809230051No Hit
TAGTCGGGGATGAAGATTTAAAGATAAAAATATTCAAAATTTTAATAACCAC1590.1060388809230051No Hit
ATTGCAGGGAGGGCCCCGGGGGCTGCGGAGGTGGCTGAGACGCGGCTGGCTG1590.1060388809230051No Hit
GAAACAGGGCCGAGAGTTCATCTGCGCTCTGTCCATCACCTCCAGGGGCAGC1580.10537196972223149No Hit
ACAACTGGGAGTGTGGCCAAGAAAATAATCTTTGCTTTGCACCAGGTTGGAG1580.10537196972223149No Hit
ATTTGAGGGATTTTAAAAAGGCATTTCACTTTTCTAGGGAAAGTTTAATGCT1580.10537196972223149No Hit
CCTTGTGGGGTGAAATTGCCCTGTGAAGTGTTTGAACGTTTTAGACCTGCAG1580.10537196972223149No Hit
AGTGGCGGGACTCGCAGCCCTTTGTCTGACTCATAGTGTAGGCTGCAGGCTC1580.10537196972223149No Hit
ATTGAAGGGCAAGCCAAGACCAAGTTAGGTTTATGTGTTTTTGGCTTAACCT1570.10470505852145787No Hit
ATGGTTGGGGAGCTCAAGTACATGAATGTTAGTTGTTATCACATACAGCAAA1570.10470505852145787No Hit
TCAGTTCGGGTATACGATGGCTTCATGTGTACAGGTAACAAATTTCATTAAT1570.10470505852145787No Hit
ACGATGGGGGCGGGGAGAGGAGTACGGGTTCAGCGTGGACTGGTGGGCGCTG1560.10403814732068425No Hit
ATCATTGGGAGAGAGGGAGGGAGGGAGAGAGAGCGCGCTAGCGCGAGAGAGC1550.10337123611991064No Hit
TTGATGGGGAAAGGGAAAATAAAGAAAAACCAATAAAAAAGAGAACGAATAC1550.10337123611991064No Hit
TATAGCTGGGAAGTCAATGGAATGGAATAGAGAGTCCAGAAATAAAGCCCCA1550.10337123611991064No Hit
AGCACTGGGGACGCTCTTCCGATCTAGAGTAGGGATTTAAATGTACTCCAGT1540.102704324919137No Hit
CCCATGGGATGGAGACCCGAAGGGACTCAGACGGAGCCGCCGTGTTGGCAGC1540.102704324919137No Hit
CTAGTTGGGGAGAGGCTAAGGTGGAAGAATCACTTGAGCTTGGGCAGTTGAG1540.102704324919137No Hit
CGGTTTGGGATAGGCACCCAGACAACTGCTGCTGCCTGCAATACTTCCTAAC1530.10203741371836339No Hit
AACTGAGGGTGGGAGTAGTAAGTGTTAACAAGTGAACTATTCATTGGGTACT1530.10203741371836339No Hit
CATCCAGGGGAAACTTTATTGAAAGACATTAAAGAAGATTTAAATACATGGG1530.10203741371836339No Hit
TCTCAAGGGATATGCTGTTTTGTAGAGTGATGACAGAGAAACAGATACAGCA1520.10137050251758978No Hit
ATTTGCGGGAAGTCTTAGGATAAACTCAAATCACAAAATATACAACAGGTAC1520.10137050251758978No Hit
TACCTGGGGTAACACACAATGGGAAAGTATTGTCTTTTCAACAAATAGTGTT1520.10137050251758978No Hit
AAGCAGGGAGAAAACAAGATGAGATTGGCATGGCTTTATTTGTTTTTTTTGT1520.10137050251758978No Hit
AGGCTAGGGAGATCAGGGGCCTAGATTTAAAAAAATGAAAAACATTATGGGT1520.10137050251758978No Hit
CGTTGAGGGAGCCGATTTTCCGGAAAAAGGCTAAAACTACAAAGAAGATTGT1510.10070359131681617No Hit
AAGTATGGGAGGCCGACCAGCACTTGGGATCTGATGGGACACGGCCAGTGCC1510.10070359131681617No Hit
AAACTGGGGCTTCTGTAACTTGTTTGGGTCATATCAGTGGTGGTTCAAGTAG1510.10070359131681617No Hit
CAGCTAGGAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCTAAAAC1510.10070359131681617No Hit
TTAGACGGGATCTAGCAAAAATATTCTTTGTAACTGAAGGAGAAATAAAGTC1500.10003668011604255No Hit
AGCTTGGGGGCTCTTTCCCTAAGCAGCCTGAGGTGATCTGTGAAAATGGTTC1500.10003668011604255No Hit
GCACAAGGGCAAACTCATAAACCAGCAAAGAGAAAAGTAATAAAAACTGTAC1500.10003668011604255No Hit
ATTGCAAGGGATCTCTATAGTACAGGGAATATAAAATTGGTTTCCTGTGGTC1500.10003668011604255No Hit
TCTGTCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1500.10003668011604255No Hit
CCTAAAGGGCAAGCCATTCTCTTCCTTTCATCTCGTAGTCAGGGAGTCCCCA1500.10003668011604255No Hit
ATCCCAGGGGAGAAGAAAACACATGCCTGTCACTCTACGGCAGCTGCCACCA1500.10003668011604255No Hit
TGTTCCGGGCAACAGAATGAAGAGACAACACACAGAATAGGAAAAAATATTT1500.10003668011604255No Hit
ATGCTTGGGTGAGTGAGCATGGGAGTGGATTCTCCAAACTCCAGTTAAGACT1500.10003668011604255No Hit
CTAATGGGGGTATTTTATTTTATCTGCTAACTAGTCTACTTCAGTGATTTAA1500.10003668011604255No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)