FastQCFastQC Report
Sat 31 Dec 2022
SRR5844729.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844729.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences202337
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCAGGACTCATCTCGTATGC8590.42453925876137333No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGGACTCATCTCGTATGCCGTCT8360.41317208419616774No Hit
CAATAGGGGAGAGACACACACAGCACTTCAGTAGCATTTGCATTCCTGGTTA2320.11466019561424752No Hit
CGGCACGGCAAGAGACAGTTTCTGTGGATGTTCCTCATCTGAAATTTTGAGA2230.11021217078438447No Hit
ACAGATGGGAGAAGCTCGCGGTGGAAGCCCTGTCCAGCCTGGACGGCGACCT2180.107741045878905No Hit
AGCATAGGGGGTCCTGCCGCAGGGCCAGAACCCCTGACGGTATTCAGCTGCG2160.10675259591671321No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)