FastQCFastQC Report
Thu 19 Jan 2023
SRR5844728.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844728.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences198785
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCAGGACTCATCTCGTATGC8860.44570767412028073No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGGACTCATCTCGTATGCCGTCT7850.3948990114948311No Hit
ACAGATGGGAGAAGCTCGCGGTGGAAGCCCTGTCCAGCCTGGACGGCGACCT2170.10916316623487687No Hit
CATTCTGGGATCGAGAGAAAGATCGTGAAAACAGCCATGGCAGGTGCTGGAC2100.1056417737756873No Hit
ATGGCCGGGGGGAGCCTCCGCCGCGCCCGCCTCCCGCCCTCTCCCCACCGGG2010.1011142691853007No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCG253.3852488E-546.0496251
TCGTTAG253.3852488E-546.0496251
CGAGTCG253.3852488E-546.0496251
ATTCGCG253.3852488E-546.0496251
TGAAACG253.3852488E-546.0496251
ACTTACG253.3852488E-546.0496251
GCTTACG253.3852488E-546.0496251
TTACGTT253.3852488E-546.0496251
CACACCG253.3852488E-546.0496251
CTTAGCG253.3852488E-546.0496251
TCGAAAG253.3852488E-546.0496251
ATTCTCG253.3852488E-546.0496251
CTTGTCG405.518814E-946.049621
TATCACG301.8398641E-646.049621
TGATACG700.046.049621
ACGTTTG206.2654045E-446.049621
CTATATG301.8398641E-646.049621
TAACGAG206.2654045E-446.049621
TATTGCG351.0053009E-746.049621
TGTGACG206.2654045E-446.049621
Produced by FastQC (version 0.11.5)