FastQCFastQC Report
Sat 31 Dec 2022
SRR5844727.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844727.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207515
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCAGGACTCATCTCGTATGC10330.49779534009589665No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGGACTCATCTCGTATGCCGTCT9560.460689588704431No Hit
ATACAAGGGAATCTTGAATAAAATATTATCCCACTTCTACAGGGGATCCAGG2790.13444811218466135No Hit
CCAAGCGGACTGTGAACATTTGTTTTCTTTTTCGTGTGTGTCAAACTAACTG2750.13252054068380598No Hit
CTACCAAGGGGTCTGATAAAAATCAATTCTAGCACTGTGAAGCTTCAGACAC2640.12721971905645377No Hit
TAGTCAGGGATTGATTTTCACTGCAGTTGAATGAACAAATTATTTCAGTGTG2600.1252921475555984No Hit
TACAGTGGGAGAACTTGTTCATTTTCCTCTTAAGTAGCTTCTGATGGGTGTC2600.1252921475555984No Hit
ATTCATGGGTAGCATAAACAAATACCAGTTTTGAGATTTGTGAATACACATT2580.1243283618051707No Hit
CACATGGGGCAGCAAGGTCTCTGGATAAAAGATTATCACCCAAATTCAGTTA2580.1243283618051707No Hit
TTATGTGGGCAATTTAAAATGTTCTCAAATCAGAAGAATTGCAAAAGAATGC2550.12288268317952919No Hit
CACACTCGGGAAGCTGATGCTGACAACATTTTTGGCAGATGGACTGTGATTT2530.1219188974291015No Hit
AATAACGGGTGTTTAGAACATTCAAGCTATTTGAAGCAATTTCCAAAATGTC2480.1195094330530323No Hit
TTAACAGCGGGGCAGTAGGCATAATTCAGCAACAAACAATCTTCCTTTGGGA2460.11854564730260464No Hit
ATTGTTGGGATATGAACTGTGTTTCCTACTTGATTAAAAAATATAAAGAACT2430.1170999686769631No Hit
TGAGTAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2420.11661807580174927No Hit
TACCTTGGGATATGTGTGAATAGTAGCTTCTGTCAATAAGCCCTCTGTAAAT2390.11517239717610775No Hit
CGCACAGGTCTGTAGGAACTTTTAGTTCCATTATATGAACACTGGATAACCT2350.11324482567525239No Hit
ACACTCGGGTCGGCCTCCCAATTAGCTGGAACTGCAGGCACACCACCTCATC2350.11324482567525239No Hit
CTGGTCAGGGCCACCATGGCTGGCGGTGCTGCTTCCTCGGGTTTGGCTGGTA2320.11179914704961087No Hit
ATTAATGGGAGCCTGGTGCATCACCACTTCCTGATGGAGAGTCAGAAGCTTC2300.11083536129918319No Hit
TGTAATTGGGTAAAATTAAAAAATTAAAAGTCAAGTCTCAAAATAGTAAAAA2280.10987157554875553No Hit
TAGGCGGGATGGTAAAGATTTAAGAAAGATCCCCTCCTCCTTTGGAAAGGGA2270.10938968267354167No Hit
CTAAAGGGGGACCAAATGTGCCTAACATCCACAGAACATTCCATCCAACAAA2270.10938968267354167No Hit
GCACAGGGCTTCGTCCGGGAGGTGAGGAGCGCCTCTGCCCGGCCGCCCTTCG2260.10890778979832784No Hit
ATAACGGGGATCGCTGTGCTCCCGGTTACCCTGGATACCCCGATGAACAGGA2250.10842589692311398No Hit
CTATGAGGGGACCTGTAATTAATCATTTGCTTCTCTTCTAATTTTGTTGTAA2250.10842589692311398No Hit
TATGTTGGGGCTTGTGATTCCTGGGTGGAAACCATGGCAGTGGACAGAAGCA2230.10746211117268632No Hit
TATCACGGGGAAGCAACTTTTTCTAGTGAGAGCTTTCCTGCTGCAGAAAACC2230.10746211117268632No Hit
TATGCGGGATTTTAATTAATAATGACCACAAAGTATAGGAGTAGTGTTGTTG2220.10698021829747247No Hit
CTCTGCATGGGTGGTTAAATAAACTACTGCACATCCTTACCAAGGGATGGTG2220.10698021829747247No Hit
TGGTTTGGGAATTAAGGTGAGTAATTAGAGAAATATTGGTTTTATCGAATGA2200.10601643254704478No Hit
AACAGCGGGGCTTTCTGTTACTGTGTTAACATTTGAGAAATGAGTATTTCAG2200.10601643254704478No Hit
CTTGGAGGGAAAATGGAGAAAATATAAAAAGATGTGAAAAAGGAAAAAGGAA2190.10553453967183095No Hit
TACGCCGGGACCTGTCTTCAACCCAAATGTTTTCAGTGAAGATGAAGATGAT2190.10553453967183095No Hit
AAAAATGGGAAGTGTTCATATTCACATAAGAGAATTTTAAATGAAGAAAATT2180.10505264679661712No Hit
TTAATTGGGACCCCCTCTCCCTGGGGCAGCTTTGCTTCCTGCCCCTGTGCTC2170.10457075392140328No Hit
GCGTTTGGGAATGTCCATATATAGAATTTGAATCATTTGACAAGGTGGGTTT2170.10457075392140328No Hit
CCAATGGGGGTGGGAAAGAGTCACTTTCTAAATCTTACCTAAAAGTTGCTAA2160.10408886104618943No Hit
TGCGCTGGGGTGGGTTCTAGTAAATACTCTAGTAAATATTTTTCTCTACTTT2120.10216128954533407No Hit
CGACCTGGGTTGAAATTATATGTTACTTTCCAATATAAATAGAGGATGAAGA2120.10216128954533407No Hit
AGTAAGGGACTGGCATAGCAGCATTCTCCCTCCTTTCCTTGAGGAATGGAAG2110.10167939667012024No Hit
TCACATGGGAGTTTGATGTATTTAGGGCAATTCTGAAATTCTGAAACACATA2110.10167939667012024No Hit
AAAACAGGGCTTTAGGGAATGGAAGTAAGTTCATTTTTAAGTTGTGTTGTCA2110.10167939667012024No Hit
AGTTGCGGGGATTCTGAAGCTATTAGAATTTTTTCCCCTGTCACAATAGCAG2110.10167939667012024No Hit
TGTTTTGGGTGCCAAGGTTTTAAGATAATTTATTGAAATCTCCTGTAAACTT2100.10119750379490638No Hit
GACATTGGGGAGCCTTGGATAAAGGGTCAGGCTCCTTTTTAGTTCAGAGATT2090.10071561091969256No Hit
AAGCGAGGGCTAGATTCAAATGGGCTGATAACCAAATTTTAACACATCAGCA2090.10071561091969256No Hit
GTTAAGGGGAGGACACGGGTCGCTGGCGGGTTAGGTGGCTGCGTGGCCGCCT2080.10023371804447873No Hit
AATCACGGGGGGTTCTCTGGGCATAAAACACATAGATGACAACAGGCGAGTG2080.10023371804447873No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)