FastQCFastQC Report
Sat 31 Dec 2022
SRR5844726.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844726.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206500
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCAGGACTCATCTCGTATGC10250.4963680387409201No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGGACTCATCTCGTATGCCGTCT8740.42324455205811135No Hit
CCAAGCGGACTGTGAACATTTGTTTTCTTTTTCGTGTGTGTCAAACTAACTG3130.15157384987893463No Hit
ATACAAGGGAATCTTGAATAAAATATTATCCCACTTCTACAGGGGATCCAGG2750.13317191283292978No Hit
CACACTCGGGAAGCTGATGCTGACAACATTTTTGGCAGATGGACTGTGATTT2690.13026634382566585No Hit
GTTAAGGGGAGGACACGGGTCGCTGGCGGGTTAGGTGGCTGCGTGGCCGCCT2640.12784503631961258No Hit
TATCACGGGGAAGCAACTTTTTCTAGTGAGAGCTTTCCTGCTGCAGAAAACC2620.12687651331719127No Hit
TTAACAGCGGGGCAGTAGGCATAATTCAGCAACAAACAATCTTCCTTTGGGA2580.12493946731234867No Hit
CACATGGGGCAGCAAGGTCTCTGGATAAAAGATTATCACCCAAATTCAGTTA2550.1234866828087167No Hit
TGTTTTGGGTGCCAAGGTTTTAAGATAATTTATTGAAATCTCCTGTAAACTT2540.12300242130750605No Hit
CGCACAGGTCTGTAGGAACTTTTAGTTCCATTATATGAACACTGGATAACCT2530.1225181598062954No Hit
AACAGCGGGGCTTTCTGTTACTGTGTTAACATTTGAGAAATGAGTATTTCAG2530.1225181598062954No Hit
CTAAAGGGGGACCAAATGTGCCTAACATCCACAGAACATTCCATCCAACAAA2520.12203389830508474No Hit
TCACAGCGGGAACTGAATAATTGTTCATAATTTCTGAAGAGCATTTAGTCTT2510.1215496368038741No Hit
ATTCATGGGTAGCATAAACAAATACCAGTTTTGAGATTTGTGAATACACATT2480.12009685230024213No Hit
AGTTGCGGGGATTCTGAAGCTATTAGAATTTTTTCCCCTGTCACAATAGCAG2420.1171912832929782No Hit
GACATTGGGGAGCCTTGGATAAAGGGTCAGGCTCCTTTTTAGTTCAGAGATT2370.11476997578692494No Hit
TAGGCGGGATGGTAAAGATTTAAGAAAGATCCCCTCCTCCTTTGGAAAGGGA2360.1142857142857143No Hit
TAAAATGGGGCCCAACCAGCCTTAATTCTTAATAAAGGAAGTTGAAAAATAA2350.11380145278450363No Hit
TCACCAGGGGAAAAACAGACACCTGAAAGTCTTCCTCAAAATAACAATCCAG2330.11283292978208233No Hit
TGTAATTGGGTAAAATTAAAAAATTAAAAGTCAAGTCTCAAAATAGTAAAAA2300.11138014527845036No Hit
ATAACGGGGATCGCTGTGCTCCCGGTTACCCTGGATACCCCGATGAACAGGA2290.11089588377723972No Hit
CACAAAGGGGTTGTAGATAGCCAGTCAACCAGCAGTATTAGTGCTGTTTTCA2290.11089588377723972No Hit
CTACCAAGGGGTCTGATAAAAATCAATTCTAGCACTGTGAAGCTTCAGACAC2270.1099273607748184No Hit
GCACAGGGCTTCGTCCGGGAGGTGAGGAGCGCCTCTGCCCGGCCGCCCTTCG2270.1099273607748184No Hit
AGAAAGGGGCAATAACACCAGTTATTTATTATTTATGAATTACAGGAGTCTG2270.1099273607748184No Hit
GCTTTGGGGTATTTACTAAAATGTAGCATATGCCAGTTATTTTAGAAAATTT2260.10944309927360775No Hit
TCGTTAGGGGATCTTTAAAGGTAGAATTTTAAATAGGCATGAAATATAGTGA2250.1089588377723971No Hit
CTATGAGGGGACCTGTAATTAATCATTTGCTTCTCTTCTAATTTTGTTGTAA2240.10847457627118644No Hit
TGCGCTGGGGTGGGTTCTAGTAAATACTCTAGTAAATATTTTTCTCTACTTT2230.1079903147699758No Hit
AAGCTAGGGGGAATACTTAGGATTTGAAAGACAATTAATCAAATTGTTTTTG2230.1079903147699758No Hit
ACACTCGGGTCGGCCTCCCAATTAGCTGGAACTGCAGGCACACCACCTCATC2220.10750605326876513No Hit
TACAGTGGGAGAACTTGTTCATTTTCCTCTTAAGTAGCTTCTGATGGGTGTC2220.10750605326876513No Hit
TACGCCGGGACCTGTCTTCAACCCAAATGTTTTCAGTGAAGATGAAGATGAT2210.10702179176755447No Hit
TAGCCTTGGGGTCTGGCGGAGAACTAGCCATCAGCCTCCTGAAGCCTGCCAT2210.10702179176755447No Hit
AAGTCGGGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGT2200.10653753026634383No Hit
TTCGGCGGGTGTTCCTTAATTTTAGCCAGAAAGCAAAAAAACAATAGTAATG2200.10653753026634383No Hit
TTATGTGGGCAATTTAAAATGTTCTCAAATCAGAAGAATTGCAAAAGAATGC2190.10605326876513319No Hit
TCAAATGGGGTACACGTTTGAAACCAACTACTGTAGAAGATGAGGAATCTAT2190.10605326876513319No Hit
AAAACAGGGCTTTAGGGAATGGAAGTAAGTTCATTTTTAAGTTGTGTTGTCA2190.10605326876513319No Hit
TTAATTGGGACCCCCTCTCCCTGGGGCAGCTTTGCTTCCTGCCCCTGTGCTC2180.10556900726392252No Hit
ACGTTTGGGATCACACCACGTCTTTTCAAAAACTAAAGAGAATTCAAAAAGG2150.10411622276029055No Hit
TCTGTGGGGCGATCTCAGTAGGGGAAAATGGTAGCTCTCTGATGAGTTTTAT2140.1036319612590799No Hit
AATAACGGGTGTTTAGAACATTCAAGCTATTTGAAGCAATTTCCAAAATGTC2140.1036319612590799No Hit
ATTAATGGGAGCCTGGTGCATCACCACTTCCTGATGGAGAGTCAGAAGCTTC2140.1036319612590799No Hit
TCACATGGGAGTTTGATGTATTTAGGGCAATTCTGAAATTCTGAAACACATA2130.10314769975786925No Hit
ATACAGGGGCGATCTTTACGGCGGGCGCCGCCGCTGCTCGGACAATTTTGCA2130.10314769975786925No Hit
TAGTCAGGGATTGATTTTCACTGCAGTTGAATGAACAAATTATTTCAGTGTG2120.1026634382566586No Hit
ATTGTTGGGATATGAACTGTGTTTCCTACTTGATTAAAAAATATAAAGAACT2100.1016949152542373No Hit
CTTATAGGGCAAGTATAAAATGTAGATCTGAGTTGGCAAACTTTCCGTAAAG2100.1016949152542373No Hit
GCCGTGGGATGGGAGAAGATCACTTTCCTTGCATGATGGATTACTAGTTTTC2100.1016949152542373No Hit
TGGCCAGGGCCGGTCGTCTCGTACCGCGAGACGGTCAGTGAAGAGTCGAACG2100.1016949152542373No Hit
AGAATAGGGTGATCTGACATTTTGCTGTTTCAAGTTTTAAAGGGCTCAAATC2090.10121065375302664No Hit
GCCAAAGAGGGGTGCAGTCATTGTGGAAAAAAGTATGAAGGTTCCTTAAGGG2090.10121065375302664No Hit
GCCAAAGGGAAGTAAACAAATCTAAGAAAAATGATATTGCAGATTATAGTGG2080.10072639225181597No Hit
TGCATAGGGAGGCATGAGCCACAATACCTGGCCAAGTCCTTTTTTTTAATCA2080.10072639225181597No Hit
TATCGTGGGTAGACAGGGATGAGGGTCATTGATCCTGGGTGCAGATCTCCAA2080.10072639225181597No Hit
CTTCATGGGATAATCCTATTTTATTAGCACAAACTTGCTAGCTAATTAGAGT2080.10072639225181597No Hit
GTTTTGGGGGCAATGACTGGAAAGTAAAGAGGACCAAGGTGAAAATAAAGGC2080.10072639225181597No Hit
CGCTGGGATGACAGCATGCCTAAAGCTAGTCTAATCTTCTCTCTGTCTTGAA2070.10024213075060533No Hit
TTCTTGGGGGCCAGTGACGTCGTGGGCTGCAGCGCAGCAGCACCCAACGCAG2070.10024213075060533No Hit
ACTAACGGGTGTCCTTCCAAGTAGAAACCTTGCAAAGTTGTAGCTTTTTAAA2070.10024213075060533No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)